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1.
Stripe rust, caused by Puccinia striiformis f.sp. tritici ( Pst), is one of the most widespread and destructive diseases of wheat worldwide. Resistance breeding is constantly pursued for decades to tackle the variations of prevalent Pst races. Zhongliang 12 has strong resistance to abiotic stresses, wide adaptability, higher resistance to stripe rust and excellent biological characteristics. To identify the resistance gene(s) against stripe rust, Zhongliang 12 was crossed with stripe rust susceptible genotype Mingxian 169, and F 1, F 2, F 2 : 3 and BC 1 progenies were tested with Chinese Pst race CYR30 and CYR31 in seedling stage in greenhouse. Zhongliang 12 possessed different dominant genes for resistance to each race. Linkage maps were constructed with four simple sequence repeats (SSRs) markers, Xwmc695, Xcfd20, Xbarc121 and Xbarc49, for the gene on wheat chromosome 7AL conferring resistance to CYR30 (temporarily designated as Yrzhong12‐1) with genetic distance ranging from 3.1 to 10.8 cM and four SSR markers, Xpsp3003, Xcfd2129, Xwmc673 and Xwmc51, for the gene on wheat chromosome 1AL conferring resistance to CYR31 (temporarily designated as Yrzhong12‐2) with genetic distance ranging from 3.9 cM to 9.3 cM. The molecular markers closely linked to each gene should be useful in marker‐assisted selection in breeding programmes for against stripe rust. 相似文献
2.
The biotrophic parasitic fungus Puccinia striiformis f. sp. tritici ( Pst) causes stripe rust, a devastating disease of wheat, endangering global food security. Because the Pst population is highly dynamic, it is difficult to develop wheat cultivars with durable and highly effective resistance. Simple sequence repeats (SSRs) are widely used as molecular markers in genetic studies to determine population structure in many organisms. However, only a small number of SSR markers have been developed for Pst. In this study, a total of 4,792 SSR loci were identified using the whole genome sequences of six isolates from different regions of the world, with a marker density of one SSR per 22.95 kb. The majority of the SSRs were di- and tri-nucleotide repeats. A database containing 1,113 SSR markers were established. Through in silico comparison, the previously reported SSR markers were found mainly in exons, whereas the SSR markers in the database were mostly in intergenic regions. Furthermore, 105 polymorphic SSR markers were confirmed in silico by their identical positions and nucleotide variations with INDELs identified among the six isolates. When 104 in silico polymorphic SSR markers were used to genotype 21 Pst isolates, 84 produced the target bands, and 82 of them were polymorphic and revealed the genetic relationships among the isolates. The results show that whole genome re-sequencing of multiple isolates provides an ideal resource for developing SSR markers, and the newly developed SSR markers are useful for genetic and population studies of the wheat stripe rust fungus. 相似文献
3.
Population genetic diversity in Tianshui city was analyzed with SSR markers in 605 single-pustule isolates of the stripe rust pathogen, Puccinia striiformis f. sp. tritici ( Pst), obtained from 19 varieties of wheat. Significant differences in genetic diversity among populations were defected. Genetic diversity was highest in population on Tian 863-13, a highly resistant variety, whereas genetic diversity was lowest in population on Huixianhong, a highly susceptible variety. Seven populations from seven varieties that carried the common Yr18 resistance gene were clustered as one sub-group at 0.88 similarity coefficient, which showed that resistance gene selection had close relation with pathogen??s component. The results of present study can provide a theoretical basis for integrated management of wheat stripe rust and effective deployment of resistance genes in Pst over-summering zones in China. 相似文献
4.
Aims: Wheat stripe (yellow) rust, caused by Puccinia striiformis f. sp. tritici ( Pst) , is the most important foliar disease on wheat in China. Early molecular diagnosis and detection of stripe rust will provide a useful aid to the accurate forecast and seasonal control of this destructive disease. Our objective was to develop PCR assays for the rapid identification and detection of P. striiformis. Methods and Results: The genomic DNA of P. striiformis and P. triticina were amplified by a pair of primers derived from conserved β‐tubulin gene sequence. A 235‐bp specific DNA fragment of P. striiformis was isolated and purified. Based on its sequence, another two primer sets were designed successfully to obtain new sequence‐characterized amplified region (SCAR) markers of P. striiformis, which could be amplified in all test isolates of P. striiformis, whereas no DNA fragment was obtained in other nontarget wheat pathogens. The detection limit of the primer set YR (f)/YR (r1) was 2·20 pg μl ?1. The new SCAR markers of P. striiformis can also be detected in Pst‐infected wheat leaves postinoculated for 2 days. Conclusions: Our assays are significantly faster than the conventional methods used in the identification of P. striiformis. Significance and Impact of the Study: Development of a simple, high‐throughput assay kit for the rapid diagnosis and detection of wheat stripe rust would be anticipated in a further study. 相似文献
5.
Stripe rust, caused by Puccinia striiformis f. sp . tritici ( Pst), is the most devastating wheat disease in China. Early and accurate detection of the pathogens would facilitate effective control of the diseases. DNA‐based methods now provide essential tools for accurate plant disease diagnosis. In this study, inter‐simple sequence repeats (ISSR) technique has been successfully applied to develop a sequence‐characterized amplified region (SCAR) marker for diagnosis of stripe rust of wheat and detection of Pst. In this study, one fragment unique to Pst was identified by ISSR and then sequenced. Based on the specific fragment, a pair of SCAR primers (616AF/616AR) was designed to amplify a 299‐bp DNA fragment within the sequenced region. The primers can amplify a unique DNA fragment for all tested isolates of Pst but not for the other pathogens of wheat leaves and the uninfected leaves. The polymerase chain reaction (PCR) assay could detect as low as 0.1 ng of genomic DNA in a 25.0 μl PCR reaction mixture and detect the pathogen from asymptomatic wheat leaves inoculated with Pst under glasshouse conditions. 相似文献
6.
Investigating the origin and dispersal pathways is instrumental to mitigate threats and economic and environmental consequences of invasive crop pathogens. In the case of Puccinia striiformis causing yellow rust on wheat, a number of economically important invasions have been reported, e.g., the spreading of two aggressive and high temperature adapted strains to three continents since 2000. The combination of sequence‐characterized amplified region (SCAR) markers, which were developed from two specific AFLP fragments, differentiated the two invasive strains, PstS1 and PstS2 from all other P. striiformis strains investigated at a worldwide level. The application of the SCAR markers on 566 isolates showed that PstS1 was present in East Africa in the early 1980s and then detected in the Americas in 2000 and in Australia in 2002. PstS2 which evolved from PstS1 became widespread in the Middle East and Central Asia. In 2000, PstS2 was detected in Europe, where it never became prevalent. Additional SSR genotyping and virulence phenotyping revealed 10 and six variants, respectively, within PstS1 and PstS2, demonstrating the evolutionary potential of the pathogen. Overall, the results suggested East Africa as the most plausible origin of the two invasive strains. The SCAR markers developed in the present study provide a rapid, inexpensive, and efficient tool to track the distribution of P. striiformis invasive strains, PstS1 and PstS2. 相似文献
7.
Stripe rust, caused by Puccinia striiformis f.sp. tritici ( Pst), is one of the most damaging diseases of wheat worldwide, especially in China. Growing resistant cultivars is the most effective approach to control the disease, but few effective resistance genes are available. Guinong 22, one of the wheat cultivars used for differentiated Chinese race of the pathogen, has unknown resistance gene(s) to stripe rust. Genetic analysis, molecular mapping and allelic analysis were used in this study to determine the inheritance and chromosomal location of the gene(s) in Guinong 22 with the most prevalent Pst race CYR33. Genetic analysis indicated that a single recessive gene yrGn22 confers the resistance to CYR33. A total of 450 simple sequence repeat (SSR) primer pairs and 31 pairs of sequence‐tagged site (STS) or conserved primers were selected to screen the resistant bulk and susceptible bulk as well as the parents. Seven polymorphic SSR markers and two STS markers were then used to genotype 113 F 2 individual plants. Linkage analysis indicated that all nine markers were linked to yrGn22, with genetic distances ranging from 2.2 to 11.1 cM. Based on the chromosomal locations of the linked markers, yrGn22 was located on wheat chromosome 1B near the centromere. The pedigree, common markers, chromosome location, resistance and allelism tests indicated that yrGn22 is either linked to Yr26 or possibly the same gene. 相似文献
9.
‘Express’, a hard red spring wheat cultivar that has been widely grown in the western United States, is used to differentiate
races of Puccinia striiformis f. sp. tritici, the causal fungal pathogen of wheat stripe rust. To identify genes conferring race-specific, overall resistance to stripe
rust, Express was crossed with ‘Avocet S’. The parents and F 1, F 2, F 3 and F 5 populations were tested with races PST-1, PST-21, PST-43, and PST-45 of P. striiformis f. sp. tritici in the seedling stage under controlled greenhouse conditions. Two dominant genes for resistance to stripe rust were identified,
one conferring resistance to PST-1 and PST-21, and the other conferring resistance to all four races. Linkage groups were
constructed for the resistance genes using 146 F 5 lines to establish resistance gene analog and chromosome-specific simple sequence repeat marker polymorphisms. The gene for
resistance to races PST-1 and PST-21 was mapped on the long arm of chromosome 1B, and that conferring resistance to all four
races was mapped on the long arm of chromosome 5B. We temporarily designate the gene on 1BL as YrExp1 and the gene on 5BL as YrExp2. Polymorphism of at least one of the two markers flanking YrExp2 was detected in 91% of the 44 tested wheat genotypes, suggesting that they would be useful in marker-assisted selection for
combining the gene with other resistance genes into many other wheat cultivars. Knowledge of these genes will be useful to
understand recent virulence changes in the pathogen populations. 相似文献
10.
Triticale introgressive lines were developed by incorporating diploid wheat ( Triticum monococcum [TM16]) genes into the hexaploid triticale genotype LT522/6. The synthetic allotetraploid T. monococcum cereale (A mA mRR) was used as a bridging form to introduce the genes. A group of 43 introgressive lines, parental stocks and a check cultivar
were inoculated at the seedling stage (in the greenhouse) and at the adult plant stage (in the field) with four pathotypes
of Puccinia striiformis f. sp. tritici to determine if the stripe rust resistance was derived from TM16 and to analyze the expression of the diploid wheat gene(s)
at the hexaploid level. At the seedling stage, 14 triticale introgressive lines expressed resistance to some of the used pathotypes,
showing introduction of a genetic material from the T. monococcum genome. Among them, 7 lines were resistant to all four stripe rust pathotypes applied at this stage. In the field, adult
plant resistance and percentage of infected leaf area were scored and transformed into the coefficient of infection. Plant
response to stripe rust was compatible at these two developmental stages with a high statistical probability showing the genetic
dependence on the same genetic background. Also observed was a full concordance of the adult plant resistance to stripe rust
with previously assessed resistance to leaf rust, as well as the highly significant linkage of the resistance to the both
diseases at the seedling stage in the set of the tested introgression lines. This result strongly suggests that T. monococcum genes responsible for these characters are located in proximity. 相似文献
11.
Wheat production in Pakistan is seriously constrained due to rust diseases and stripe rust (yellow) caused by Puccinia striiformis f. sp. tritici, which could limit yields. Thus development and cultivation of genetically diverse and resistant varieties is the most sustainable
solution to overcome these diseases. The first objective of the present study was to evaluate 100 Pakistan wheat cultivars
that have been grown over the past 60 years. These cultivars were inoculated at the seedling stage with two virulent stripe
rust isolates from the United States and two from Pakistan. None of the wheat cultivars were resistant to all tested stripe
rust isolates, and 16% of cultivars were susceptible to the four isolates at the seedling stage. The data indicated that none
of the Pakistan wheat cultivars contained either Yr5 or Yr15 genes that were considered to be effective against most P. striiformis f. sp. tritici isolates from around the world. Several Pakistan wheat cultivars may have gene Yr10, which is effective against isolate PST-127 but ineffective against PST-116. It is also possible that these cultivars may
have other previously unidentified genes or gene combinations. The second objective was to evaluate the 100 Pakistan wheat
cultivars for stripe rust resistance during natural epidemics in Pakistan and Washington State, USA. It was found that a higher
frequency of resistance was present under field conditions compared with greenhouse conditions. Thirty genotypes (30% of germplasms)
were found to have a potentially high temperature adult plant (HTAP) resistance. The third objective was to determine the
genetic diversity in Pakistan wheat germplasms using molecular markers. This study was based on DNA fingerprinting using resistance
gene analog polymorphism (RGAP) marker analysis. The highest polymorphism detected with RGAP primer pairs was 40%, 50% and
57% with a mean polymorphism of 36%. A total of 22 RGAP markers were obtained in this study. RGAP, simple sequence repeat
(SSR) and sequence tagged site (STS) markers were used to determine the presence and absence of some important stripe rust
resistance genes, such as Yr5, Yr8, Yr9, Yr15 and Yr18. Of the 60 cultivars analyzed, 17% of cultivars showed a RGAP marker band for Yr9 and 12% of cultivars exhibited the Yr18 marker band. No marker band was detected for Yr5, Yr8 and Yr15, indicating a likely absence of these genes in the tested Pakistan wheat cultivars. Cluster analysis based on molecular and
stripe rust reaction data is useful in identifying considerable genetic diversity among Pakistan wheat cultivars. The resistant
germplasms identified with 22 RGAP markers and from the resistance evaluations should be useful in developing new wheat cultivars
with stripe rust resistance. 相似文献
12.
Stripe rust (or yellow rust), caused by the fungus Puccinia striiformis f. sp. tritici ( Pst), is one of the most important foliar diseases of wheat. Characterization and utilization of novel resistant genes is the most effective, economic and environmentally friendly approach to controlling the disease. Wheat line LM168-1, which was derived from a cross between common wheat Chuannong 16 and Milan, has good adult-plant resistance to stripe rust, based on field tests over several years. To elucidate the genetic basis of resistance, LM168-1 was crossed with susceptible variety SY95-71. Parents and F1, F2, BC1 and F2:3 progenies were tested in 2009–2011 in a field inoculated with the predominant races of Pst in China. The genetic analysis showed that resistance to stripe rust in LM168-1 was controlled by a single recessive gene, temporarily designated yrLM168. Simple sequence repeat (SSR), resistance gene analog polymorphism (RGAP) and target region amplification polymorphism (TRAP) techniques were used to identify molecular markers linked to the resistance locus. Finally, a linkage group consisting of two SSR, four RGAP and five TRAP markers was constructed for yrLM168 with 102 F2 plants. The closest markers R1 and R2 flanked the resistance gene locus at 2.4 and 2.4 cM, respectively. Furthermore, two SSR markers Xwmc59 and Xwmc145 assigned the gene to chromosome 6A. Because yrLM168 confers high-level resistance to the predominant races of Pst in China, it should be useful in stripe rust resistance breeding programs. The closely linked markers can be used for rapidly transferring yrLM168 to wheat breeding populations. 相似文献
13.
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most destructive diseases of wheat worldwide. Growing resistant cultivars is the most effective approach to
control the disease, but only a few genes confer effective all-stage resistance against the current populations of the pathogen
worldwide. It is urgent to identify new genes for diversifying sources of resistance genes and for pyramiding genes for different
types of resistance in order to achieve high levels of durable resistance for sustainable control of stripe rust. The common
spring wheat genotype ‘PI 181434’, originally from Afghanistan, was resistant in all greenhouse and field tests in our previous
studies. To identify the resistance gene(s) PI 181434 was crossed with susceptible genotype ‘Avocet Susceptible’. Adult plants
of 103 F 2 progeny were tested in the field under the natural infection of P. striiformis f. sp. tritici. Seedlings of the parents, F 2 and F 3 were tested with races PST-100 and PST-127 of the pathogen under controlled greenhouse conditions. The genetic study showed
that PI 181434 has a single dominant gene conferring all-stage resistance. Resistance gene analog polymorphism (RGAP) and
simple sequence repeat (SSR) techniques were used to identify molecular markers linked to the gene. A linkage map of 8 RGAP
and 2 SSR markers was constructed for the gene using data from the 103 F 2 plants and their derived F 3 lines tested in the greenhouse. Amplification of the complete set of nulli-tetrasomic lines and selected ditelosomic lines
of Chinese Spring with an RGAP marker and the two SSR markers mapped the gene on the long arm of chromosome 3D. Because it
is the first gene for stripe rust resistance mapped on chromosome 3DL and different from all previously named Yr genes, the gene in PI 181434 was designated Yr45. Polymorphism rates of the two closest flanking markers, Xwgp115 and Xwgp118, in 45 wheat genotypes were 73.3 and 82.2%, respectively. Single nucleotide polymorphisms (SNPs) were identified in the eight
wheat genotypes sharing both flanking markers. The RGAP markers and potential SNP markers should be useful in incorporating
the gene into wheat cultivars and in pyramiding it with other genes for durable resistance. 相似文献
14.
Wild emmer wheat, Triticum dicoccoides, the progenitor of modern tetraploid and hexaploid wheats, is an important resource for new variability for disease resistance genes. T. dicoccoides accession pau4656 showed resistance against prevailing leaf rust and stripe rust races in India and was used for developing stable introgression lines (IL) in T. durum cv Bijaga yellow and named as IL pau16068. F5 Recombinant inbred lines (F5 RILs) were developed by crossing IL pau16068 with T. durum cultivar PBW114 and RIL population was screened against highly virulent Pt and Pst pathotypes at the seedling and adult plant stages. Inheritance analyses revealed that population segregated for two genes for all stage resistance (ASR) against leaf rust, one ASR gene against stripe rust and three adult plant resistance (APR) genes for stripe rust resistance.
For mapping these genes a set of 483 SSR marker was used for bulked segregant analysis. The markers showing diagnostic polymorphism in the resistant and susceptible bulks were amplified on all RILs. Single marker analysis placed all stage leaf rust resistance genes on chromosome 6A and 2A linked to the SSR markers Xwmc256 and Wpaus268, respectively. Likewise one all stage stripe rust resistance gene were mapped on long arm of chromosome 6A linked to markers 6AL-5833645 and 6AL-5824654 and two APR genes mapped on chromosomes 2A and 2B close to the SSR marker Wpaus268 and Xbarc70, respectively. The current study identified valuable leaf rust and stripe rust resistance genes effective against multiple rust races for deployment in the wheat breeding programme. 相似文献
15.
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars is the preferred means
of control of the disease. The winter wheat cultivar Xiaoyan 54 has high-temperature resistance to stripe rust. To identify
genes for stripe rust resistance, Xiaoyan 54 was crossed with Mingxian 169, a winter wheat genotype susceptible to all Chinese
races of the pathogen. Seedlings and adult plants of the parents and F 1, F 2, F 3 and F 4 progeny were tested with Chinese race CYR32 under controlled greenhouse conditions and in the field. Xiaoyan 54 has two recessive
resistance genes, designated as Yrxy1 and Yrxy2, conferring high-temperature resistance. Simple sequence repeat (SSR) primers were used to identify molecular markers flanking
Yrxy2 using 181 plants from one segregating F 3 line. A total of nine markers, two of which flanked the locus at genetic distances of 4.0 and 6.4 cM on the long arm of chromosome
2A were identified. Resistance gene analog polymorphism (RGAP) and SSR techniques were used to identify molecular markers
linked to Yrxy1. A linkage group of nine RGAP and two SSR markers was constructed for Yrxy1 using 177 plants of another segregating F 3 line. Two RGAP markers were closely linked to the locus with genetic distances of 2.3 and 3.5 cM. Amplification of a set
of nulli-tetrasomic Chinese Spring lines with RGAP markers M8 and M9 and the two SSR markers located Yrxy1 on the short arm of chromosome 7A. The SSR markers Xbarc49 and Xwmc422 were 15.8 and 26.1 cM, respectively, from the gene. The closely linked molecular markers should be useful for incorporating
the resistance genes into commercial cultivars and combining them with other genes for stripe rust resistance. 相似文献
16.
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most widespread and destructive wheat diseases worldwide. Growing resistant cultivars is the preferred control
of the disease. The spring wheat cultivar ‘Alpowa’ has both race-specific, all-stage resistance and non-race-specific, high-temperature
adult-plant (HTAP) resistances to stripe rust. To identify genes for the stripe rust resistances, Alpowa was crossed with
‘Avocet Susceptible’ (AVS). Seedlings of the parents, and F 1, F 2 and F 3 progeny were tested with races PST-1 and PST-21 of P. striiformis f. sp. tritici under controlled greenhouse conditions. Alpowa has a single partially dominant gene, designated as YrAlp, conferring all-stage resistance. Resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques
were used to identify molecular markers linked to YrAlp. A linkage group of five RGAP markers and two SSR markers was constructed for YrAlp using 136 F 3 lines. Amplification of a set of nulli-tetrasomic Chinese Spring lines with RGAP markers Xwgp47 and Xwgp48 and the two SSR markers indicated that YrAlp is located on the short arm of chromosome 1B. To map quantitative trait loci (QTLs) for the non-race-specific HTAP resistance,
the parents and 136 F 3 lines were tested at two sites near Pullman and one site near Mount Vernon, Washington, under naturally infected conditions.
A major HTAP QTL was consistently detected across environments and was located on chromosome 7BL. Because of its chromosomal
location and the non-race-specific nature of the HTAP resistance, this gene is different from previously described genes for
adult-plant resistance, and is therefore designated Yr39. The gene contributed to 64.2% of the total variation of relative area under disease progress curve (AUDPC) data and 59.1%
of the total variation of infection type data recorded at the heading-flowering stages. Two RGAP markers, Xwgp36 and Xwgp45 with the highest R
2 values were closely linked to Yr39, should be useful for incorporation of the non-race-specific resistance gene into new cultivars and for combining Yr39 with other genes for durable and high-level resistance. 相似文献
17.
Stripe rust, caused by Puccinia striiformis f. sp. tritici, is one of the most important diseases of wheat worldwide. The best strategy to control stripe rust is to grow resistant cultivars. One such cultivar resistant to most races in North America is ‘IDO377s’. To study the genetics of its resistance this spring wheat cultivar was crossed with ‘Avocet Susceptible’ (AvS). Seedlings of the parents, F 2 plants, and F 3 lines were tested under controlled greenhouse conditions with races PST-43 and PST-45 of P. striiformis f. sp. tritici. IDO377s carries a single dominant gene for resistance. Resistance gene analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to identify molecular markers linked to the resistance gene. A total of ten markers were identified, two of which flanked the locus at 4.4 and 5.5 cM. These flanking RGAP markers were located on chromosome 2B with nulli-tetrasomic lines of ‘Chinese Spring’. Their presence in the ditelosomic 2BL line localized them to the long arm. The chromosomal location of the resistance gene was further confirmed with two 2BL-specific SSR markers and a sequence tagged site (STS) marker previously mapped to 2BL. Based on the chromosomal location, reactions to various races of the pathogen and tests of allelism, the IDO377s gene is different from all previously designated genes for stripe rust resistance, and is therefore designated Yr43. A total of 108 wheat breeding lines and cultivars with IDO377s or related cultivars in their parentage were assayed to assess the status of the closest flanking markers and to select lines carrying Yr43. The results showed that the flanking markers were reliable for assisting selection of breeding lines carrying the resistance gene. A linked stripe rust resistance gene, previously identified as YrZak, in cultivar Zak was designated Yr44. 相似文献
19.
Puccinia striiformis f. sp. tritici ( Pst), the causal fungus of wheat stripe rust, was previously reported to be infected by Lecanicillium lecanii, Microdochium nivale and Typhula idahoensis. Here, we report a novel hyperparasite on Pst. This hyperparasitic fungus was identified as Cladosporium cladosporioides (Fresen.) GA de Vries based on morphological characteristics observed by light and scanning electron microscopy together with molecular data. The hyperparasite reduced the production and viability of urediniospores and, therefore, could potentially be used for biological control of wheat stripe rust. 相似文献
20.
Stripe rust, caused by Puccinia striiformis f. sp. tritici ( Pst), is one of the most devastating diseases worldwide and is also an important disease in China. The wheat translocation line H9014-121-5-5-9 was originally developed from interspecific hybridization between wheat ( Triticum aestivum L.) line 7182 and Psathyrostachys huashanica Keng. This translocation line showed resistance to predominant stripe rust races in China when it was tested with nine races of Pst. To determine the inheritance and map the resistance gene, segregating populations were developed from the cross between H9014-121-5-5-9 and the susceptible cultivar Mingxian 169. The seedlings of the F1, F2, and F2:3 generations were tested with race CYR31. The results showed that the resistance in H9014-121-5-5-9 was conferred by a single dominant gene. Bulked segregant analysis and simple sequence repeat (SSR) markers were used to identify polymorphic markers associated with the resistance gene locus. Seven polymorphic SSR markers were linked to the resistance gene. A linkage map was constructed according to the genotypes of the seven SSR markers and the resistance gene. Based on the SSR marker positions on the wheat chromosome, the resistance gene was assigned on chromosome 1AL, temporarily designated YrHA. Based on chromosomal location, reaction patterns and pedigree analysis, YrHA should be a novel resistance gene to stripe rust. The molecular markers of the new resistance gene in H9014-121-5-5-9 could be useful for marker-assisted selection in breeding programs against stripe rust. 相似文献
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