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1.
喹诺酮类药物抗乙型肝炎病毒体外实验研究   总被引:4,自引:0,他引:4  
本文以2.2.15细胞株为模型,以HBsAg、HBeAg、HBVDNA、细胞存活率为观察指标,综合评价了喹诺酮类药物吡哌酸(PipemidicAcid)、氟哌酸(Norfloxacin)、环丙氟哌酸(Ciproflosxacin)、氟嗪酸(Ofloxacin)体外抗HBV效果。结果表明:吡哌酸、氟哌酸、环丙氟哌酸、氟嗪酸对HBsAg、HBeAg50%抑制浓度(ID_(50))分别为11μg/ml、64μg/ml、93μg/ml、105μg/ml和199μg/ml、111μg/ml、24μg/ml、217μg/ml,细胞存活率为50%时的药物浓度(CD_(50))分别为219μg/ml、90μg/ml、181μg/ml、169μg/ml,在所选定的用药浓度范围内不同程度抑制培养上清液及细胞内HBVDNA及其复制中间体的产生。尤其对超螺旋结构DNA(scDNA)有不完全抑制作用。  相似文献   

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用套式多聚酶链反应(Nested-PCR)技术对169对HBsAg及HBsAg/HBeAg阳性孕妇及其新生儿外周血清进行了HBV-DNA检测,103对HBsAg阳性孕妇及其新生儿外周务中HBV-DNA阳性率分别为72.8%和33.0%;66对HBsAg和HBeAg双阳性的孕妇及其新生儿外周血清中HBV-DNA阳性率分别为86.4%和43.9%,对55例HBsAg及HBsAg/HBeAg阳性产妇产后  相似文献   

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用套式多聚酶链反应(Nested-PCR)技术对169对HBsAg及HBsAg/HBeAg阳性孕妇及其新生儿外周血清进行了HBV-DNA检测。103对HBsAg阳性孕妇及其新生儿外周血清中HBV-DNA阳性率分别为72.8%和33.0%;66对HBsAg和HBeAg双阳性的孕妇及其新生儿外周血清中HBV-DNA阳性率分别为86.4%和43.9%。对55例HBsAg及HBsAg/HBeAg阳性产妇产后的初乳进行了HBV-DNA检测,结果HBV-DNA阳性率为36.4%。结果表明HBsAg和HBeAg双阳性的孕妇及其新生儿外周血清HBV-DNA检出率较HBsAg单阳性的孕妇及其新生儿要高,其初乳中HBV-DNA的检出率也高。还对105例注射了乙肝疫苗及高价乙肝特异性免疫球蛋白的6月龄婴儿的外周血清进行了HBV-DNA检测,结果有23例阳性。  相似文献   

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应用ELISA和PCR法检测502例乙肝病人血清,401例HBsAg阳性血清中,有114例(28.4%)抗-HCV和HCVRNA双项阳性,25例(6.2%)HCVRNA单项阳性;21例(5.2%)抗-HCV单项阳性。将HBsAg乙肝病人分成HBVDNA,HBeAg阳性组和HBVDNA,HBeAg阴性组。前者抗-HCV阳性率为11.6%~20.5%,HCVRNA阳性率为16.2%~20.5%。后者抗-HCV阳性率为20.2%~55.6%,HCVRNA阳性率为23%~60.3%。结果说明长期携带HBV者和慢性乙肝病人均可重叠HCV感染。HBVDNA阳性组抗-HCV和HCVRNA阳性率明显高于HBVDNA阳性组  相似文献   

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HRP-HBVDNA探针在临检应用中的研究   总被引:2,自引:0,他引:2  
本文介绍了一种简便的检测血清HBVDNA的方法。参照Renz等人的标记方法,构建了直接酶标HRP HBVDNA探针。此探针经与固定在硝酸纤维素滤膜上的血清靶DNA杂交后,可通过化学发光自显影检测技术观察结果。敏感度可检测0-1pg靶DNA,相当于同位素探针的灵敏度。对63份HBsAgHBeAg和Anti HBcELISA阳性血清以及24份HBsAgAnti HBc阳性,HbeAg阴性血清用HRP HBVDNA探针进行检测,结果探针HBVDNA阳性率分别为100%(63)和58%(14);对50份HBsAg,ELISA阴性和ALT正常的血清,探针HBVDNA全部阴性。实验结果表明本方法具有很大的推广应用价值。  相似文献   

6.
设计合成了两个分别互补于乙肝病毒2.1kb mRNA起始区(片段A)和增强子区(片段B)的硫代磷酸的DNA片段,在经克隆HBV DNA转染HepG2细胞建立的HBV短暂表达系统及稳定产生HBV的2215细胞中研究二者对HBsAg及HBeAg表达的抑制作用。结果表明反义寡聚物能不同程序抑制乙肝抗原表达,并与剂量呈一定正相关。在HepG2细胞HBV短暂表达系统中,6μmol/L浓度时,片段A、B对HB  相似文献   

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对362名无症状高滴度HBsAg携带者用RIA法检测HBeAg、Anti-HBe、Anti-HCV、Anti-HDV。结果表明,HBeAg阳性率随HBsAg滴度的增高而增加,且有显著性差异(P<0.05)。重叠感染以HBV+HCV最高,占27.6%;其次是HBV+HDV,占9.1%;HBV+HCV+HDV最少,占6.4%。但是,以上重叠感染率均与HBsAg滴度及/或HBeAg阳性率高低无关(P>0.05)。调查显示,无症状HBsAg携带者中HBV+HCV,或HBV+HDV,或HBV+HCV+HDV的重叠感染均可发生。  相似文献   

8.
大球母贝精子介导外源基因转移研究   总被引:9,自引:0,他引:9  
将大球母贝(Pinctada maxima Jameson)精子与“全鱼”GH基因重组体pCAgcGH和pCAgcGHc的线性DNA混合,温育30min,经6次、2^7、10kV脉冲电处理后,与卵子受精,得到若干贝苗。从贝苗中提取DNA,经PCR扩增和Southern blot分子杂交表明,部分受体带有外源基因,当与精子温育的外源基因浓度分别为2μg/mL,6μg/mL及18μg/mL时,相应贝苗  相似文献   

9.
本文采用间接免疫荧光法(IF),RPHA法,ELISA法及斑点杂交技术检测10例无症状HB-sAg携带者及89例乙肝病人尿细胞中的HBsAg、HBeAg及HBVDNA,发现尿细胞中有HBsAg、HBeAg、HBVDNA存在。结果提示:乙肝无症状携带者及乙肝病人尿细胞中具有HBsAg、HBeAg、HBVDNA,因此更进一步证实尿液具有传染性。  相似文献   

10.
为从全基因组水平研究乙型肝炎病毒(HBV)的核苷酸结构及复制和抗原表达特性,分别从2例HBsAg和HBeAg阳性、HBV DNA滴度为10^14和10^13拷贝/ml的慢性乙型肝炎患者(编号为56和2-18)血清中扩增、克隆了HBV全基因组,并测定了核苷酸序列。两株病毒基因组转染HepG2细胞的培养上清中HBsAg水平基本相同,但#56毒株表达的HBeAg约为#2-18株的3倍,Southerm印  相似文献   

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It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.  相似文献   

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Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.  相似文献   

17.
Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.  相似文献   

18.
肝癌中HBV和HCV基因和抗原的分布及意义   总被引:1,自引:0,他引:1  
采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细  相似文献   

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For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.  相似文献   

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