共查询到20条相似文献,搜索用时 15 毫秒
1.
2.
3.
Xueyan Ren Qingjun Kong Peng Wang Feng Jiang Huili Wang Ting Yu Xiaodong Zheng 《Molecular biology reports》2011,38(2):801-807
LePR-5, a putative PR5 like protein gene was amplified from a cherry tomato (Lycopersicon esculentum), which encodes a precursor protein of 250 amino acid residues, and shares high degrees of homology with a number of other
PR5 genes. Expression of LePR-5 in different tomato organs was analyzed with Semi-quantitative RT–PCR, showing that LePR-5
expressed at different levels in leaves, stems, roots, flowers and fruits. In addition, expression of LePR-5 under different
abiotic stresses was carried out at different time points. Three of the four tested abiotic stimuli, ethophen, salicylic acid
and methyl jasmonate, triggered a significant induction of LePR-5 after treatment. However, LePR-5 was weaker induced by abscisic
acid than by others. The positive responses of LePR-5 to the three abiotic stimuli suggested that LePR-5 may play an important
role in response to abiotic stresses, and it may also be involved in plant defense system against pathogens. In addition,
different expression patterns between tomato fruit and seedling suggested that LePR-5 may play a distinctive role in the defensive
system protecting tomato fruit and seedling. 相似文献
4.
Sesuvium portulacastrum, a mangrove plant from seashore, is a halophyte species well adapted to salinity and drought. Some efforts have been made
to describe its physiological and structural characteristics on salt and drought-tolerance, but the underlying molecular mechanism
and key components have not yet been identified. Here, a fructose-1,6-bisphosphate aldolase gene, designated SpFBA, was isolated and characterized from S. portulacastrum roots in response to seawater. The SpFBA cDNA has a total length of 1452 bp with an open reading frame of 1071 bp, and is predicted to encode a precursor protein
of 357 amino acid residues sharing high degree of homology with class I FBAs from other plants. Semi-quantitative RT-PCR analysis
indicated that the SpFBA was more strongly expressed in roots than in leaves and stems, and the abiotic stimuli such as Seawater, NaCl, ABA, and PEG,
could trigger a significant induction of SpFBA in S. portulacastrum roots within 2–12 h. Overproduction of Recombinant SpFBA resulted in an increased tolerance to salinity in transgenic Escherichia coli. All these results suggest that the SpFBA plays very important roles in responding to salt stress and related abiotic stimuli, and in improving the survival ability
of S. portulacastrum under high salinity and drought.
The GenBank Accession number of S. portulacastrum fructose-1,6-bisphosphate aldolase (SpFBA) is ACG68894. 相似文献
5.
6.
Ruiling Liu Meiqin Liu Jie Liu Yuzhen Chen Yiyin Chen Cunfu Lu 《Plant Growth Regulation》2010,60(2):163-168
A late embryogenesis abundant protein gene, AmLEA from Ammopiptanthus mongolicus, was introduced into Escherichia coli using the IMPACT™-TWIN system to analyze the possible function of AmLEA under heat and cold stresses. A fusion protein about 38 kD was expressed in E.coli cells harboring pTWIN-LEA after the induction of IPTG by SDS–PAGE analysis and the accumulation of the fusion protein peaked
3 h after IPTG addition when cultured at 37°C. Compared with control cells, the E. coli cells expressing AmLEA fusion protein showed improved chilling and heat resistence, illuminating the protein may play a protective
role in cells under stress conditions. These results suggested the natively unstructured protein, similar to other members
of LEA proteins, has high capacity for binding water and potential protective function against dehydration or action similar
to the cold shock chaperones. 相似文献
7.
8.
9.
Caiqiu Gao Yucheng Wang Guifeng Liu Chao Wang Jing Jiang Chuanping Yang 《Plant Molecular Biology Reporter》2010,28(1):77-89
Plant peroxidases (PODs) have been ascribed a variety of biological functions, including hydrogen peroxide detoxification,
lignin biosynthesis, hormonal signaling, and stress response. In the present study, ten POD genes, including three ascorbate peroxidases (class I PODs) and seven secretory peroxidases (class III PODs), were cloned from Tamarix hispida. The roles of the ten POD genes were addressed under different abiotic stress conditions, and gene expression profiles in roots, stems, and leaves
were evaluated using real-time quantitative reverse-transcribed polymerase chain reaction. Our results showed that the relative
abundance of the PODs was markedly different in roots, stems, and leaves, indicating that POD activity differs in these three organs. ThPOD1 and ThPOD8 were the most and least abundant, respectively, in all organs. The expression profiles in response to abiotic stresses were
organ specific. All of the genes were highly induced by drought, salt, salt–alkaline, CdCl2, and abscisic acid (ABA) treatments in at least one organ. Five ThPOD genes were induced in roots, stems, and leaves under all of the studied stress conditions, indicating that they are closely
associated with abiotic stress. Our results demonstrate that the ten plant peroxidases are all expressed in leaves, stems,
and roots, that they are involved in different abiotic stress responses, and that they are controlled by an ABA-dependent
stress signaling pathway. 相似文献
10.
Dehydrins (DHNs) compose a family of intrinsically unstructured proteins that have high water solubility and accumulate during late seed development at low temperature or in water-deficit conditions. They are believed to play a protective role in freezing and drought-tolerance in plants. A full-length cDNA encoding DHN (designated as ClDhn) was isolated from an oriental medicinal plant Codonopsis lanceolata, which has been used widely in Asia for its anticancer and anti-inflammatory properties. The full-length cDNA of ClDhn was 813 bp and contained a 477 bp open reading frame (ORF) encoding a polypeptide of 159 amino acids. Deduced ClDhn protein had high similarities with other plant DHNs. RT-PCR analysis showed that different abiotic stresses such as salt, wounding, chilling and light, triggered a significant induction of ClDhn at different time points within 4-48 hrs post-treatment. This study revealed that ClDhn assisted C. lanceolata in becoming resistant to dehydration. 相似文献
11.
Yong Gao Yun Zhao Tingting Li Caixia Ren Yang Liu Maolin Wang 《Plant Molecular Biology Reporter》2010,28(3):450-459
A full-length cDNA encoding a putative G protein β subunit (Gβ), designated as BnGB1, was isolated from Brassica napus. BnGB1 was predicted to encode a precursor protein of 378-amino acid residues. The expression of BnGB1 in different B. napus tissues and developmental stages was analyzed using real-time polymerase chain reaction. The results showed that BnGB1 expressed was high at the seventh day, the bolting stage, and fruiting stage. Moreover, BnGB1 was analyzed under four different plant hormones. All of the four tested hormones, abscisic acid (ABA), GA3, brassinosteroid (BR), and indole-3-acetic acid (IAA), triggered an induction of BnGB1 at different hormone concentrations. The expression of BnGB1 was induced by low ABA concentrations, but high ABA concentrations inhibited the expression, which demonstrated that there
might be an ABA-mediated feedback repression of the BnGB1 expression. BnGB1 was more prominently induced by high concentrations of GA3 than by other plant hormones. The positive responses stimuli suggested that BnGB1 might be involved in signaling pathways for response to high concentrations of GA. However, the expression of BnGB1 did not significantly regulated by exogenous BR and IAA compared with the level regulated by ABA and GA3. In addition, BnGB1 was responsive to different abiotic stresses and displayed differential expression patterns in B. napus. The BnGB1 was upregulated in salt and drought stress and downregulated in heat and cold stress. These results suggest that
BnGB1 plays an important role in ABA and GA signal pathways and may also be involved in plant defense system against environmental
stresses in B. napus. 相似文献
12.
13.
Monodehydroascorbate reductase (MDHAR; EC 1.6.5.4) catalyses the reduction of the monodehydroascorbate (MDHA) radical to ascorbate,
using NADH or NADPH as an electron donor, and is believed to be involved in maintaining the reactive oxygen scavenging capability
of plant cells. This key enzyme in the ascorbate-glutathione cycle has been studied here in the moss Physcomitrella patens, which is tolerant to a range of abiotic stresses and is increasingly used as a model plant. In the present study, three
cDNAs encoding different MDHAR isoforms of 47 kDa were identified in P. patens, and found to exhibit enzymic characteristics similar to MDHARs in vascular plants despite low-sequence identity and a distant
evolutionary relationship between the species. The three cDNAs for the P. patens MDHAR enzymes were expressed in Escherichia coli and the active enzymes were purified and characterized. Each recombinant protein displayed an absorbance spectrum typical
of flavoenzymes and contained a single non-covalently bound FAD coenzyme molecule. The K
m and k
cat values for the heterologously expressed PpMDHAR enzymes ranged from 8 to 18 μM and 120–130 s−1, respectively, using NADH as the electron donor. The K
m values were at least an order of magnitude higher for NADPH. The K
m values for the MDHA radical were ∼0.5–1.0 μM for each of the purified enzymes, and further kinetic analyses indicated that
PpMDHARs follow a ‘ping–pong’ kinetic mechanism. In contrast to previously published data, site-directed mutagenesis indicated
that the conserved cysteine residue is not directly involved in the reduction of MDHA. 相似文献
14.
Yang L Tang R Zhu J Liu H Mueller-Roeber B Xia H Zhang H 《Plant molecular biology》2008,66(4):329-343
Inositol phosphates (IPs) and their turnover products have been implicated to play important roles in stress signaling in
eukaryotic cells. In higher plants genes encoding inositol polyphosphate kinases have been identified previously, but their
physiological functions have not been fully resolved. Here we expressed Arabidopsis inositol polyphosphate 6-/3-kinase (AtIpk2β) in two heterologous systems, i.e. the yeast Saccharomyces
cerevisiae and in tobacco (Nicotiana tabacum), and tested the effect on abiotic stress tolerance. Expression of AtIpk2β rescued the salt-, osmotic- and temperature-sensitive growth defects of a yeast mutant strain (arg82Δ) that lacks inositol polyphosphate multikinase activity encoded by the ARG82/IPK2 gene. Transgenic tobacco plants constitutively expressing AtIpk2β under the control of the Cauliflower Mosaic Virus 35S promoter were generated and found to exhibit improved tolerance to
diverse abiotic stresses when compared to wild type plants. Expression patterns of various stress responsive genes were enhanced,
and the activities of anti-oxidative enzymes were elevated in transgenic plants, suggesting a possible involvement of AtIpk2β
in plant stress responses.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
15.
Animal cells react to mitogenic or stress stimuli by rapid up-regulation of immediate-early (IE) genes and a parallel increase
in characteristic modifications of core histones: chromatin changes, collectively termed the nucleosomal response. With regard
to plants little is known about the accompanying changes at the chromatin level. We have used tobacco BY-2 and Arabidopsis T87 cell lines to study the nucleosomal response of plant cells to high salinity, cold and exogenous abscisic acid (ABA).
When in quiescent stage, both tobacco and Arabidopsis cells show the typical nucleosomal response to high salinity and cold stress, manifested by rapid transient up-regulation
of histone H3 Ser-10 phosphorylation, immediately followed by transient up-regulation of H3 phosphoacetylation and histone
H4 acetylation. For each of the studied stresses the observed nucleosomal response was strictly correlated with the induction
of stress-type specific genes. The dynamics of histone modifications in BY-2 cells in response to exogenous ABA exhibited
a more complex pattern than that evoked by the two abiotic stresses, probably due to superposition of the primary and secondary
effects of ABA. A rapid increase in H3 Ser-10 phosphorylation was also observed in whole leaves subjected to high salinity;
however, the rate of change in this modification was much slower than in cultured cells. Together, these results indicate
that the quiescent BY-2 and T87 cell lines show a typical nucleosomal response to abiotic stresses and ABA treatment and may
represent suitable models for the study of chromatin-mediated mechanisms of stress tolerance in plants. 相似文献
16.
植物特异性转录因子NAM家族从属于NAC转录因子超家族,在植株生长发育、生理代谢以及应对各种胁迫反应中均发挥重要作用。该研究采用生物信息学方法鉴定水稻基因组中的NAM基因,分析其时空表达模式、亚细胞定位以及蛋白相互作用,并采用实时定量qRT PCR方法分析不同外源激素(如SA、ABA和MeJA)以及非生物胁迫(包括干旱、盐和冷)处理下各NAM基因的表达特征,为进一步探索NAM基因在非生物胁迫中的功能和应激机制以及激素调控途径奠定基础。结果显示:(1)从水稻基因组中共鉴定出48个NAM基因,进化分析将其分为5个亚家族;NAM基因在水稻基因组中存在9对片段复制事件。(2)组织表达分析显示,NAM基因在水稻不同组织及发育时期表现特异性表达,特别是叶鞘、茎和节的生长过程中高表达,且大多数是核定位,并存在多种蛋白互作。(3)实时定量qRT PCR表达分析显示,10个NAM基因在不同组织中均特异表达;大部分NAM基因在盐和干旱胁迫下表达上调,而在冷胁迫下表达降低;SA、ABA和MeJA处理均可显著改变各NAM基因的表达水平。研究表明,NAM基因在水稻生长发育、激素应答和非生物胁迫响应中具有重要作用。 相似文献
17.
L. Ge J. Z. Liu W. S. Wong W. L. W. Hsiao K. Chong Z. K. Xu S. F. Yang S. D. Kung N. Li 《Plant, cell & environment》2000,23(11):1169-1182
Many abiotic environmental factors elicit the production of stress‐ethylene in higher plants. To elucidate the molecular mechanisms underlying the regulation of stress‐ethylene production and the physiological roles played by stress‐ethylene in stress responses of plants, we studied the gene expression of ACC synthase in tobacco plants that had been subjected to environmental stresses. Four new tobacco ACC synthase cDNA fragments, NT‐ACS2, NT‐ACS3, NT‐ACS4 and NT‐ACS5, were identified and sequenced. It was found that NT‐ACS2 could be induced by wounding, cold temperature and, especially, sunlight. NT‐ACS4 was induced at a faster kinetics by wounding. The multiple environmental stress‐responsive (MESR) NT‐ACS2 gene was found to contain three introns and four exons and encode a polypeptide of 484 amino acids, 54·6 kDa and pI 6·87. Computer analysis of the 3·4 kb 5 ′ flanking region upstream of the ACS coding region revealed the existence of a group of putative cis‐acting regulatory elements potentially conferring wounding, chilling, and UV light inducibility. Phylogenetic analysis of ACC synthase genes of different plant origins indicated that the chill‐inducible NT‐ACS2 gene is closely related to a chilling‐inducible citrus ACS gene. 相似文献
18.
In this study, the expression patterns of four ferritin genes (PpFer1, PpFer2, PpFer3, and PpFer4) in pear were investigated using quantitative real-time PCR. Analysis of tissue-specific expression revealed higher expression
level of these genes in leaves than in other tested tissues. These ferritin genes were differentially expressed in response
to various abiotic stresses and hormones treatments. The expression of ferritin wasn’t affected by Fe(III)-citrate treatment.
Abscisic acid significantly enhanced the expression of all four ferritin genes, especially PpFer2, followed by N-benzylyminopurine, gibberellic acid, and indole-3-acetic acid. The expression peaks of PpFer1 and PpFer3 in leaves appeared at 6, 6, and 12 h, respectively, after pear plant was exposed to oxidative stress (5 mM H2O2), salt stress (200 mM NaCl), and heat stress (40°C). A significant increase in PpFer4 expression was detected at 6 h after salt stress or heat stress. The expression of ferritin genes was not altered by cold
stress. These results suggested that ferritin genes might be functionally important in acclimation of pear to salt and oxidative
stresses. Hormone treatments had no significant effect on expression of ferritin genes compared to abiotic stresses. This
showed accumulation of ferritin genes could be operated by different transduction pathways under abiotic stresses and hormones
treatments. 相似文献
19.
Ortiz-Masia D Perez-Amador MA Carbonell P Aniento F Carbonell J Marcote MJ 《Planta》2008,227(6):1333-1342
Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as in biotic and
abiotic stress responses. They are classified according to their sequence homology into four major groups (A–D). A large amount
of information about MAPKs in groups A and B is available but few data of the C group have been reported. In this study, a
C1 subgroup MAP kinase cDNA, PsMPK2, was isolated from Pisum sativum. PsMPK2 is expressed in vegetative (root and leaf) and reproductive (stamen, pistil and fruit) organs. Expression of PsMPK2 in Arabidopsis thaliana shows that mechanical injury and other stress signals as abscisic acid, jasmonic acid and hydrogen peroxide increase its
kinase activity, extending previous results indicating that C1 subgroup MAPKs may be involved in the response to stress. 相似文献
20.
Flavonols are produced by the desaturation of dihydroflavanols, which is catalyzed by flavonol synthase (FLS). FLS belongs
to the 2-oxoglutarate iron-dependent oxygenase family. The full-length cDNA and genomic DNA sequences of the FLS gene (designated as GbFLS) were isolated from Ginkgo biloba. The full-length cDNA of GbFLS contained a 1023-bp open reading frame encoding a 340-amino-acid protein. The GbFLS genomic DNA had three exons and two introns. The deduced GbFLS protein showed high identities with other plant FLSs. The
conserved amino acids (H–X–D) ligating ferrous iron and residues (R–X–S) participating in 2-oxoglutarate binding were found
in GbFLS at similar positions like other FLSs. GbFLS was found to be expressed in all tested tissues including roots, stems,
leaves, and fruits. Expression profiling analyses revealed that GbFLS expression was induced by all of the six tested abiotic stresses, namely, UV-B, abscisic acid, cold, sucrose, salicylic acid,
and ethephon, consistent with the in silico analysis results of the promoter region. The recombinant protein was successfully
expressed in the E. coli strain BL21 (DE3) with a pET-28a vector. The in vitro enzyme activity assay by high performance liquid chromatography indicated
that recombinant GbFLS protein could catalyze the formation of dihydrokaempferol to kaempferol and the conversion of kaempferol
from naringenin, suggesting that GbFLS is a bifunctional enzyme within the flavonol biosynthetic pathway. 相似文献