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1.
Jie Sun Mehrigul Abdulahat Ablikim Obolda Xuelian Ma Parida Hazretomar Zhaoze Ding Jinquan Wang 《Luminescence》2024,39(1):e4680
As an oxidant, deodorant and bleaching agent, the hypochlorous acid (HClO) and hypochlorite (ClO−) are widely used in corrosion inhibitors, textile dyes, pharmaceutical intermediates and in our daily lives. However, excess usage or aberrant accumulation of ClO− leads to tissue damage or some diseases and even cancer. Therefore, it is necessary to develop a fluorescent probe that specifically identifies ClO−. In this article, we synthesized a deep-red xanthene-based fluorescent probe (XA-CN). The strong electron deficient group dicyano endows the probe XA-CN deep-red fluorescent emission with high solubility, selectivity and sensitivity for ClO− detection. Studies showed that the probe demonstrated turn-off fluorescence (643 nm) at the presence of ClO− in dimethylsulfoxide/phosphate-buffered saline 1:1 (pH 9) solution with a limit of detection of 1.64 μM. Detection mechanism investigation revealed that the electron deficient group -CN and the hydroxyl group was oxidized into aldehyde or carbonyl groups at the presence of ClO−, resulting ultraviolet-visible absorption of the probe blue shifted and turned-off fluorescence. Furthermore, XA-CN was successfully used for the detection of ClO− in tap water samples. 相似文献
2.
Pu Chen Qingli Nie Yuting Yan Juncheng Yang Hai-Tao Feng Ben Zhong Tang 《Luminescence》2024,39(1):e4600
Hypochlorite (ClO−), as a kind of essential reactive oxygen species, plays a crucial role in vitro and in vivo. Here, a ratiometric fluorescent probe ( TPAM ) was designed and constructed for sensing ClO− based on substituted triphenylamine and malononitrile, which exhibited obvious colour transfer from orange to colourless under daylight accompanied by noticeable fluorescence change from red to green in response to ClO−. TPAM could effectively monitor ClO− with the merits of fast response, excellent selectivity, high sensitivity and a low detection limit of 0.1014 μM. 1H NMR, mass spectra and theoretical calculations proved that ClO− caused the oxidation of the carbon–carbon double bond in TPAM , resulting in compound 1 and marked changes in colour and fluorescence. In addition, TPAM was utilized for imaging ClO− in living cells successfully with good photostability and biocompatibility. 相似文献
3.
Russell K. Hynes 《FEMS microbiology letters》1987,41(2):181-184
A high-pressure liquid chromatography (HPLC) technique, previously developed for nitrite (NO−2) and nitrate (NO−3) measurements [3], was used to measure chlorite (ClO−2) production by Nitrobacter winogradskyi. The determination of ClO−2 by HPLC involves monitoring the column effluent with a UV detector at 214 or 254 nm. Although the absorbance of ClO−2 at 214 nm was about 5 times greater than at 254 nm, interference from other compounds in the culture filtrates of N. winogradskyi contributed to an unstable detector signal. The detection limit at 254 nm for ClO−2 in deionized water was about 1 μM.The measurement of ClO−2 in N. winogradskyi culture filtrates was done with detection at 254 nm. The maximum concentration of ClO−2 produced by anaerobically incubated cell suspensions of N. winogradskyi was about 80 μM. 相似文献
4.
Hypochlorous acid and its hypochlorite are important reactive oxygen species in the body, and are involved in various physiological processes related to immunity; their rapid detection is of great significance. Here, we synthesized a fluorescent probe (TPAS) by condensation of 4-(diphenylamino)benzaldehyde, carbohydrazide, and salicylaldehyde, which can be used for the detection of ClO− in water and sensing of acidic gas in its solid state. The probe showed strong selective recognition of ClO− in acetonitrile and good tolerance to interference ions. There were good linear responses between the intensity of absorbance and fluorescence and the amount of ClO−. The TPAS solid and its paper strips can emit red fluorescence when exposed to volatile acidic vapours. After being treated with NH3, the red fluorescence can be restored to yellow. The response process of TPAS to ClO− and acid gases was characterized using nuclear magnetic resonance, electrospray ionisation mass spectrometry, transmission electron microscopy, and density functional theory calculations. Furthermore, it can be utilized in analyzing ClO− in commercially available bleaching products; the detection results were basically compatible with the labelled values. In addition, the probe is biocompatible and can be applied for imaging ClO− in zebrafish. 相似文献
5.
《Biochimica et Biophysica Acta (BBA) - Enzymology》1978,522(1):151-160
In the presence of 10 μM Ca2+ and 5 mM Mg2+ (or 0.25 mM Mg2+), the addition of 100 μM Zn2+, Ni2+, Co2+, Fe2+, Cu2+ or 1 mM Mn2+ resulted in varying degrees of stimulation or inhibition of 10−6 M cyclic GMP and cyclic AMP hydrolysis by the activator-dependent cyclic nucleotide phosphodiesterase from bovine heart in the absence or presence of phosphodiesterase activator. The substrate specificity of the enzyme was altered under several conditions. The addition of Zn2+ in the presence of 5 mM Mg2+ and the absence of activator resulted in the stimulation of cyclic GMP hydrolysis over a narrow substrate range while reducing the V 65% due to a shift in the kinetics from non-linear with Mg2+ alone to linear in the presence of Zn2+ and Mg2+. Zn2+ inhibited the hydrolysis of cyclic GMP and cyclic AMP in the presence of activator with Ki values of 70 and 100 μM, respectively. Zn2+ inhibition was non-competitive with substrate, activator and Ca2+ but was competitive with Mg2+. In the presence of 10 μM Ca2+ and activator, a Ki of 15 μM for Zn2+ vs. Mg2+ was noted in the hydrolysis of 10−6 M cyclic GMP. Several effects of Zn2+ are discussed which have been noted in other studies and might be due in part to changes in cyclic nucleotide levels following phosphodiesterase inhibition. 相似文献
6.
The mononuclear dipeptide‐based CuII complexes [CuII(trp‐phe)(phen)(H2O)] ⋅ ClO4 ( 1 ) and [CuII(trp‐phe)(bpy)(H2O)] ⋅ ClO4 ( 2 ) (trp‐phe=tryptophanphenylalanine, phen=1,10‐phenanthroline, bpy=2,2′‐bipyridine) were isolated, and their interaction with DNA was studied. They exhibit intercalative mode of interaction with DNA. The intercalative interaction was quantified by Stern Volmer quenching constant (Ksq=0.14 for 1 and 0.08 for 2 ). The CuII complexes convert supercoiled plasmid DNA into its nicked circular form hydrolytically at physiological conditions at a concentration as low as 5 μM (for 1 ) and 10 μM (for 2 ). The DNA hydrolysis rates at a complex concentration of 50 μM were determined as 1.74 h−1 (R=0.985) for 1 and 0.65 h−1 (R=0.965) for 2 . The rate enhancement in the range of 2.40–4.10×107‐fold compared to non‐catalyzed double‐stranded DNA is significant. This was attributed to the presence of a H2O molecule in the axial position of the Cu complexes. 相似文献
7.
UiO-66-NH2 nanocomposite was post-modified with 4-mercaptophenylboronic acid (MPBA) by the method of in situ hybridization reaction. The hybrid boronate affinity material UiO-NH2@P (TEPIC-co-MPBA) was characterized by scanning electron microscopy, X-ray diffraction and Fourier-transform infrared spectroscopy. The material was applied as fluorescent probe for the detection of cis-diol containing compounds based on the boronate affinity mechanism, and exhibited high specific selectively. The proposed method exhibited good linearity for the detection of catechol in the range of 0.50 to 8.00 μg ml−1. The detection limit was 0.13 μg ml−1. The tactic was successfully applied to analyze the total polyphenols in tea beverages for catechol, and relative recovery was in 98.86–106.00%. Therefore, this work provided a promising strategy for the recognition of cis-diol containing compounds. 相似文献
8.
The sensitive and reliable detection of Hg2+ and CN− as harsh environmental contaminants are of great importance. In view of this, a novel ‘on–off–on’ fluorescent probe based on nitrogen-rich silicon quantum dots (NR-SiQDs) has been designed for sensitive detection of Hg2+ and CN− ions in aqueous medium. NR-SiQDs were synthesized using a facile, one-step, and environment friendly procedure in the presence of 3-aminopropyl trimethoxysilane (APTMS) and ascorbic acid (AA) as precursors, with l -asparagine as a nitrogen source for surface modification. The NR-SiQDs exhibited strong fluorescence emission at 450 nm with 42.34% quantum yield, satisfactory salt tolerance, and superior photostability and pH stability. The fluorescence emission was effectively quenched using Hg2+ (turn-off) due to the formation of a nonfluorescent stable NR-SiQDs/Hg2+ complex, whereas after the addition of cyanide ions (CN−), Hg2+ ions could be leached from the surface of the NR-SiQDs and the fluorescence emission intensity of the quenched NR-SiQDs fully recovered (turn-on) due to the formation of highly stable [Hg(CN)4]2− species. After optimizing the response conditions, the obtained limits of detection were found to be 53 nM and 0.46 μM for Hg2+ and CN−, respectively. Finally, the NR-SiQD-based fluorescence probe was utilized to detect Hg2+ and CN− ions in water samples and satisfactory results were obtained, suggesting its potential application for environmental monitoring. 相似文献
9.
Folic acid (FA) also known as (N-[p-{[(2-amino-4-hydroxy-6-pteridinyl) methyl] amino} benzoyl]-l-glutamic acid), is a water soluble vitamin found in plants and animals. The deficiency of FA leads to an increased risk of various diseases like neural tube defects in newborn, cardiovascular disease, cancer, Alzheimer’s disease and megaloblastic anemia. The normal levels of FA in human blood serum should fall in the range between 2 and 15 ng/mL. Present review article discusses the classification, principles, advantages and disadvantages of FA biosensing methods. FA biosensors operate within 3–300 s, in pH range, 1.8–7.8, concentration range 8.71 × 10−9 μM for FA. The FA biosensors displayed detection limits (LOD) between 1.6 × 10−11 to 0.091 μM and with working potential −0.88 to 4.5 V. These biosensors measured FA level in various biological and pharmaceuticals samples. 相似文献
10.
A luminous intensity variation fluorescent probe (Probe 1) for the detection of ferric ion was developed. The quantitative range of Fe3+ content detected was 0–600 μM with the limit of detection at 0.76 μM. Furthermore, after 20 min of Fe3+ addition, the intensity of the luminescence of Probe 1 solution gradually decreased with increase in Fe3+ concentration. In addition, the B and G values of these images showed a linear relationship with Fe3+ concentration (0–500 μM). Probe 1 was successfully used for the rapid determination of Fe3+ concentration in real samples. This study demonstrates that Probe 1 is an excellent tool for the rapid determination of Fe3+ content in real samples using a smart phone without professional equipment. 相似文献
11.
Weiwei Yuan Shuhuai Li Xionghui Ma Chaohai Pang Yuwei Wu Mingyue Wang Bei Li 《Luminescence》2024,39(2):e4651
The luminescence performance of fluorescent reagents plays a crucial role in fluorescence analysis. Therefore, in this study, a novel bi-ligand Zn-based metal–organic framework, Au nanoparticle (NP) fluorescent material was synthesized using a hydrothermal method with Zn as the metal source. Simultaneously, a DNA aptamer was introduced as a molecular recognition element to develop a Zn-based MOF@Au NPs/DNA aptamer fluorescent probe for the ultra-trace detection of thiamethoxam residues in agricultural products. The probe captured different concentrations of the target molecule, thiamethoxam, through the DNA aptamer, causing a conformational change in the DNA aptamer and bursting the fluorescence of the probe, therefore establishing a fluorometric method for thiamethoxam detection. This method is highly sensitive due to the excellent luminescence properties of the Zn-based MOF@Au NPs, and the DNA aptamer can specifically recognize thiamethoxam, offering high selectivity. The linear range of the method was 2.5–6000 × 10−11 mol L−1, with a detection limit of 8.33 × 10−12 mol L−1. This method was applied to the determination of actual samples, such as bananas, and the spiked recovery rate was found to be in the range 84.05–109.07%. Overall, the proposed probe has high sensitivity, high selectivity, and easy operation for the detection of thiamethoxam residues in actual samples. 相似文献
12.
A highly sensitive and selective fluorescent probe for fluoride anions based on intramolecular charge transfer
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Jingkai Liu Zhenghe Xu Caiyun Liu Lirong Xu Zhongpeng Wang Baocun Zhu 《Luminescence》2016,31(5):1166-1170
Currently, there is a great need to develop methods for the selective detection of fluoride anions (F–) owing to their toxicity in the environment and biological function in living systems. In this study, we developed a new fluorescent probe (probe 1) employing a Si–O bond as a highly selective recognition receptor for detecting F– via intramolecular charge transfer. Probe 1 could detect F– quantitatively using the turn‐on fluorescence spectroscopy method with excellent sensitivity in the range of 4–38 μM and a detection limit of 0.26 μM; the detection time was < 17 min. We anticipate that probe 1 would be used widely to monitor F– in the environment. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
13.
Fatimah A. M. Al-Zahrani Huda A. Al-Ghamdi Mohamed A. Abdel-Lateef Reda M. El-Shishtawy 《Luminescence》2023,38(4):477-486
A sensitive and selective phenothiazine-based sensor (PTZ) has been successfully synthesized. The sensor PTZ displayed specific identification of CN− ‘turn-off’ fluorescence responses with a quick reaction and strong reversibility in an acetonitrile:water (90:10, V/V) solution. The sensor PTZ for detecting CN− exhibits the marked advantages of quenching the fluorescence intensity, fast response time (60 s), and low value of the detection limit. The concentration that is authorized for drinking water by the WHO (1.9 μM) is far higher than the detection limit, which was found to be 9.11 × 10−9. The sensor displays distinct colorimetric and spectrofluorometric detection for CN− anion due to the addition of CN− anion to the electron-deficient vinyl group of PTZ, which reduces intramolecular charge transfer efficiencies. The 1:2 binding mechanism of PTZ with CN− was validated by fluorescence titration, Job's plot, HRMS, 1H NMR, FTIR analysis, and density functional theory (DFT) investigations, among other methods. Additionally, the PTZ sensor was successfully used to precisely and accurately detect cyanide anions in actual water samples. 相似文献
14.
J. P. Macmanus A. L. Boynton J. F. Whitfield D. J. Gillan R. J. Isaacs 《Journal of cellular physiology》1975,85(2):321-329
Low (5 × 10−9 M to 10−7 M) acetylcholine concentrations cause a calcium-independent stimulation of the initiation of DNA synthesis and proliferation of lymphoblasts which are part of rat thymocyte populations suspended in vitro. A much higher (5 × 10−5 M) acetylcholine concentration also stimulates lymphoblast DNA synthesis and proliferation, but this action is calcium-dependent. This proliferogenic response to acetylcholine is however not clearly mediated by either cyclic GMP or cyclic AMP. 相似文献
15.
Jasminder Sahi Michael P. Wiggins Gil B. Gibori Thomas J. Layden Mrinalini C. Rao 《Journal of cellular physiology》1996,168(2):276-283
To determine if calcium-dependent secretagogues directly act on epithelial cells to elicit CI− secretion, their effects on CI− transport and intracellular Ca2+ concentrations ([Ca2+]i) were determined in primary cultures of rabbit distal colonic crypt cells. The Cl− sensitive fluorescent probe, 6-methoxyquinolyl acetoethyl ester, MQAE and the Ca2+-sensitive fluorescent probe, fura-2AM were used to assess Cl− transport and [Ca2+]i, respectively. Basal Cl− transport (0.274 ± 0.09 mM/sec) was inhibited significantly by the Cl− channel blocker diphenylamine-2-carboxylate (DPC, 50 μM, 0.068 ± 0.02 mM/sec; P < 0.001) and the Na+/K+/2Cl− cotransport inhibitor furosemide (1 μM, 0.137 ± 0.04 mM/sec; P < 0.01). Ion substitution studies using different halides revealed the basal influx to be I− > F− ≥ Cl− > Br−. DPC inhibited I− influx by ∼50%, F− influx by 80%, Cl− influx by 85%, and Br− influx by 90%. Furosemide significantly inhibited influx of Br− (84%) and Cl− (81%) but not of F− and I−. The effects of agents known to alter biological response by increasing [Ca2+]i in other epithelial systems were used to stimulate Cl− transport. Cl− influx in mM/second was stimulated by 1 μM histamine (0.58 ± 0.05), 10 μM neurotensin (2.07 ± 0.32), 1 μM serotonin (1.63 ± 0.28), and 0.1 μM of the Ca2+ ionophore A23187 (2.05 ± 0.40). The Cl− permeability stimulated by neurotensin, serotonin, and A23187 was partially blocked by DPC or furosemide added alone or in combination. Histamine-induced Cl− influx was significantly inhibited by only furosemide. Indomethacin blocked histamine-stimulated Cl− permeability but had no effect on the actions of the other agents. These studies, focusing on isolated colonocytes without the contribution of submucosal elements, reveal that (1) histamine stimulates Cl− transport by activating the Na+/K+/2Cl− cotransporter via a cyclooxygenase-dependent pathway; (2) neurotensin, serotonin, and A23187 activate both Cl− channels and the cotransporter, and their actions are cyclooxygenase-independent. © 1996 Wiley-Liss, Inc. 相似文献
16.
《Inorganica chimica acta》1986,123(1):27-33
Complexes of xanthine (xnH) with 3d metal perchlorates were prepared by refluxing mixtures of ligand and metal salt in ethyl acetate-triethyl orthoformate. In all cases, partial substitution of anionic xn− for ClO4− groups occurs, and the solid complexes isolated also contain invariably two neutral xnH ligands per metal ion, viz. Cr(xn)2(xnH)2ClO4, Fe(xn)2(xnH)2ClO4·H2O, M(xn)(xnH)2ClO4·H2O (M = Fe, Co, Ni) and M(xn)(xnH)2ClO4· 2H2O (M = Mn, Zn). The new complexes are generally hexacoordinated and appear to be linear chainlike polymeric species characterized by a (-Mxn-)n single-bridged backbone. Four terminal ligands per metal ion, including two xnH groups in all cases, complete its inner coordination sphere; the remaining two terminal ligands differ from complex to complex as follows: M = Cr3+ xn−, -OClO3; Fe3+ xn−, H2O; Fe2+, Co2+, Ni2+OClO3, H2O; Mn2+, Zn2+ two aqua ligands. Probable binding sites of bidentate bridging xn− and unidentate terminal xnH and xn− are discussed. 相似文献
17.
Jing Xu Yanfeng Shi Gongwei Xu Qinrui Zhao Li Hui Shuo Zhang Baocun Zhu Zhenghe Xu Zhen Bian 《Luminescence》2022,37(5):729-733
Developing an effective method for the detection of nitrite (NO2−) ions in the natural environment especially in environmental waters and soils is very necessary, since they will cause serious damage to human health once excess NO2− ions enters the human body. Therefore, a new colorimetric fluorescent probe NB-NO2− for determining NO2− ions was designed, which possesses good water-solubility and satisfactory selectivity over other common ions for NO2− ions. The addition of NO2− ions changed the color of solution from blue to colorless seen by the naked-eye. Furthermore, through test and calculation, the detection limit of the probe NB-NO2− is 129 nM. Based on the earlier excellent characteristics, the probe NB-NO2− was successfully used for monitoring NO2− ions in environmental waters and soils. 相似文献
18.
We developed a facile strategy for the fabrication of red fluorescent carbon nanodots (R-CDs) and demonstrated their applications for Al3+ sensing. Red-emission carbon dots (CDs) were synthesized using a simple hydrothermal treatment with citric acid and urea as precursors, manifesting intriguing red-emission behaviour at 610 nm. With increasing Al3+ concentration, the fluorescence band at 610 nm decreased gradually. Monitoring the intrinsic fluorescence variation (I610nm), as-prepared CDs were developed as an effective platform for fluorescent Al3+ sensing, with a linear range of 0.5–60.0 μM and a detection limit of 3.0 nM. More importantly, R-CDs have been applied successfully to the analysis of Al3+ in actual samples with satisfactory recoveries in the range 97.12–102.05%, which indicated that obtained CDs could be implemented as an effective tool for the identification and detection of Al3+ in actual samples. 相似文献
19.
Biswanath Hansda Biplab Mondal Soumyajit Hazra Arindam Banerjee 《Journal of peptide science》2023,29(10):e3492
A dipeptide-appended perylenediimide (PDI-CFF) fluorescent molecule was designed, synthesized, and characterized. Though the molecule does not dissolve in any individual solvent, it dissolves well in an organic/water mixed solvent system such as tetrahydrofuran/water. This new fluorescent molecule was self-assembled in a tetrahydrofuran/water mixture to form both nanofibrous network structures and a nano ring structure. It has shown nanofibril morphology by the interactions with ferric ions (PDI-CFF/Fe3+ system) with diminishing fluorescent property. Interestingly, L-ascorbic acid (LAA) interacts with the PDI-CFF/Fe3+ system, showing turn-on fluorescence. Another interesting feature is that the minimum detection limits for Fe3+ ions and LAA are at the submicromolar levels of 6.2 × 10−8 and 3 × 10−8 M, respectively. Moreover, the fluorescent (10 μM) signals can be monitored by the naked eye under handheld UV lamp irradiation at 365 nm, and this is very convenient for the real application. In this study, the molecule offers the opportunity for processing these sequential fluorescence responses in order to fabricate a implication logic gate that includes NOT, AND, and OR simple logic gates using chemical stimuli (ferric ions and LAA) as inputs and fluorescence emission at 536 nm as output. The detailed mechanism of interactions of Fe3+ with PDI-CFF and LAA with the PDI-CFF/Fe3+ system is vividly studied by using Fourier transform infrared (FT-IR) analysis and fluorescence. Moreover, this new molecule was reusable for several times without significant loss of its activity. The construction of logic gates using biologically important molecules/ions holds future promise for the design and development of new bio-logic gates. 相似文献
20.
Qi Wang Bin Zhu Yejiao Han Xin Yang Yanan Xu Ying Cheng Taotao Liu Jiana Wu Shengling Li Lifeng Ding Jingjing Bai Yulan Niu 《Luminescence》2022,37(8):1267-1274
In this work, carbon dots (CDs) was easily synthesized from aspartic acid through a pyrolysis method. Based on their favourable fluorescence properties, CDs were utilized to design a metal ion-mediated fluorescent probe for N-acetyl-l -cysteine (NAC) detection. The fluorescence intensity of CDs was firstly quenched by manganese ions (Mn2+) through static quenching effect and subsequently restored by NAC via the combination with Mn2+ due to the coordination effect. Therefore, the fluorescent turn-on sensing of NAC was actuated based on the fluorescence quenching stimulated by Mn2+ and recovery induced by coordination. The fluorescence recovery efficiencies showed a proportional range to the concentration of NAC in the range 0.04–5 mmol L−1 and the detection limit was 0.03 mmol L−1. Furthermore, this metal ion-mediated fluorescent nanoprobe was applied to human urine sample detection and the standard recovery rates were located in the range 97.62–102.34%. This was the first time that Mn2+ was used to construct a fluorescent nanoprobe for NAC. Compared with other heavy metal ions, Mn2+ with good biosecurity prevented the risk of application, which made the nanoprobe green and biopractical. The facile synthesis of CDs and novel metal ion-mediated sensing mode made it a promising method for pharmaceutical analysis. 相似文献