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Dynamic Relation between Expansion and Cellular Turgor in Growing Grape (Vitis vinifera L.) Leaves 总被引:4,自引:4,他引:4 下载免费PDF全文
Measurements of the growth and water relations of expanding grape (Vitis vinifera L.) leaves have been used to determine the relationship between leaf expansion rate and leaf cell turgor. Direct measurement of turgor on the small (approximately 15 micrometer diameter) epidermal cells over the midvein of expanding grape leaves was made possible by improvements in the pressure probe technique. Leaf expansion rate and leaf water status were perturbed by environmentally induced changes in plant transpiration. After establishing a steady state growth rate, a step decrease in plant transpiration resulted in a rapid and large increase in leaf cell turgor (0.25 megapascal in 5 minutes), and leaf expansion rate. Subsequently, leaf expansion rate returned to the original steady state rate with no change in cell turgor. These results indicate that the expansion rate of leaves may not be strongly related to the turgor of the leaf cells, and that substantial control of leaf expansion rate, despite changes in turgor, may be part of normal plant function. It is suggested that a strictly physical interpretation of the parameters most commonly used to describe the relationship between turgor and growth in plant cells (cell wall extensibility and yield threshold) may be inappropriate when considering the process of plant cell expansion. 相似文献
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Isaura Castro Olinda Pinto-Carnide Jesús María Ortiz Juan Pedro Martín 《Molecular biotechnology》2013,54(2):528-540
Grapevine chloroplast (cp) DNA diversity was analysed for the first time through amplification and digestion of fragments of the large single copy (LSC) region by polymerase chain reaction–restriction fragment length polymorphism methodology and also by amplification of three microsatellite loci, previously described as polymorphic in grapevine. Thirty-eight grapevine cultivars collected mainly in the North of Portugal, including some neglected cultivars, four international cultivars (Chasselas, Muscat of Alexandria, Muscat of Hamburg and Pinot) and Vitis riparia and Vitis rupestris, were used in this study with the main goal of finding out their cpDNA diversity and compare the obtained results with previously published data on cultivars from other regions to ascertain their possible origin. Two different alleles were found in each of the three cpSSR loci. Allele variants of the three loci combined in a total of three different haplotypes (A, B and D). The most frequent haplotype, A, was previously reported as the most frequent in Iberian Peninsula and Occidental Europe. Haplotype B was unique to Rabigato, Muscat of Alexandria, V. riparia and V. rupestris. This haplotype was previously proposed to be an ancestral haplotype. Twenty-seven fragments of the LSC region of Vitis vinifera cpDNA were amplified and then digested with HinfI and TaqI restriction enzymes. Polymorphisms were found in the trnT-psbC (TC) and orf184-petA (OA) fragments. In the TC fragment, the polymorphism corresponds to a point mutation in a restriction site of TaqI and is only present in all cultivars with cpSSR haplotype D. In the OA fragment, a short deletion exclusive to the Rabigato cultivar was found. In this case, one sequence tagged site-based marker was developed and will be very useful in future phylogenetic and fingerprinting studies in a broader number of cultivars and in wild grapevine populations. Inference about the progenitors of the Touriga Franca cultivar is done. The present work supports and completes its origin as a descendent of the female and male parents, Marufo and Touriga Nacional. 相似文献
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采用正交设计L9(34)对影响葡萄ISSR-PCR反应体系的4个因素(dNTP、TaqDNA聚合酶、引物、模板DNA)在3个浓度水平上进行试验,并通过直观分析初步确定其反应体系;在此基础上,通过单因素试验探讨了dNTP、TaqDNA聚合酶、引物、模板DNA、退火温度及循环次数等因素或条件对葡萄ISSR-PCR扩增结果的影响,确定最佳反应水平。最终建立了葡萄ISSR-PCR扩增的最佳反应体系:在25μL的反应体系中,dNTP浓度0.2 mmol/L,TaqDNA聚合酶的用量0.5 U,引物浓度0.4mmol/L,DNA模板用量40 ng。反应程序:94℃预变性5 min;94℃变性1 min,52℃退火1 min,72℃延伸1 min 30 s,40次循环;最后72℃延伸10 min,10℃保存。 相似文献
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Thermotolerance and related antioxidant enzyme activities induced by both heat acclimation and exogenous salicylic acid (SA)
application were studied in grapevine (Vitis vinifera L. cv. Jingxiu). Heat acclimation and exogenous SA application induced comparable changes in thermotolerance, ascorbic acid
(AsA), glutathione (GSH), and hydrogen peroxide (H2O2) concentrations, and in activities of the antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), glutathione reductase
(GR), ascorbic peroxidase (APX) and catalase (CAT) in grape leaves. Within 1 h at 38 °C, free SA concentration in leaves rose
from 3.1 μg g−1 FW to 19.1 μg g−1 FW, then sharply declined. SA application and heat acclimation induced thermotolerance were related to changes of antioxidant
enzyme activities and antioxidant concentration, indicating a role for endogenous SA in heat acclimation in grape leaves. 相似文献
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B. R. Bondada 《Journal of Plant Growth Regulation》2011,30(2):185-198
The anatomical and micro-morphological alterations as induced by the auxinic herbicide, 2,4-D (2,4-dichlorophenoxy acetic
acid) have not yet been elucidated for a commercially important fruit crop such as grapevine despite its super sensitivity
to 2,4-D. Light and scanning electron microscopy techniques were employed to examine 2,4-D induced internal and external structural
abnormalities in Merlot grapevines (Vitis vinifera L.). Healthy leaves were dorsiventrally flattened with well developed patterns of cellular structure and composition involving
adaxial palisade parenchyma and abaxial spongy mesophyll. Dorsiventral variations in epidermal features involved large epidermal
cells on the adaxial surface, and trichomes and stomata with turgid elliptical guard cells on the abaxial surface. The 2,4-D
injured leaves were small and enated; the veins were fasciated with rugose bands of lamina existing between fasciated veins.
The epidermal cells aggregated instead of being positioned coplanar to the epidermal plane. The adaxial elongated palisade
parenchyma cells were transformed into an ovoid shape with intercellular spaces. An extensive development of replacement tissues
took place on the abaxial surface wherein the stomata became roundish and were either raised or sunken with collapsed and
cracked guard cells that developed abnormal outer stomatal ledges. These abnormalities are expected to severely perturb the
vital functions of photosynthesis and transpiration ultimately leading to vine death attributable, at least in part, to the
injured leaves. 相似文献
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In woody cuttings of the grape vine inflorescences of fertilebuds usually atrophy soon after bud burst. Inflorescences areretained if leaves are removed from the elongating shoot, butremoval of apices and roots does not affect inflorescence growthif leaves are present. With defoliated cuttings, inflorescencegrowth is stimulated by removal of apices but depressed by removalof roots. Applications of indole acetic acid to the petiolestumps of defoliated cuttings did not replace the effect ofleaves and induce atrophy of inflorescences. Inflorescence growthin defoliated cuttings was inhibited by 2,4-dichlorophenoxyaceticacid (2,4-D), but concentrations which depressed inflorescencegrowth produced toxicity symptoms. Applications of 2,3,5-tri-iodobenzoicacid to petioles and leaf laminae were without effect on inflorescencegrowth. Treatment of inflorescences with the cytokinin 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine(SD 8339) promoted inflorescence growth in both the presenceand the absence of leaves. Inhibitory effects of 2,4-D on inflorescencegrowth were partially reversible by application of SD 8339.Response of inflorescences to gibberellic acid required eitherremoval of leaves or addition of SD 8339. 相似文献
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Sweet William J.; Morrison Janice C.; Labavitch John M.; Matthews Mark A. 《Plant & cell physiology》1990,31(4):407-414
The rate and composition of cell wall polysaccharide synthesisduring development and growth-inhibiting water deficits wereinvestigated in leaves of grape (Vitis vinifera L.). The rateof leaf expansion was monitored as plant water status was manipulatedby modulating the supply of irrigation water to potted plantsover several days. The corresponding wall synthesis was determinedby incubating leaf tissue with [14C]glucose and quantifyingincorporation into wall components. Samples were obtained fromrapidly expanding and mature leaves before, during, and following(recovery from) moderate water deficits. Uptake was approximately2-fold greater for mature leaf tissue than for rapidly expandingtissue at both high and low water status. In contrast, incorporationinto cell wall polysaccharides was 18 to 41% (under low andhigh water status) of uptake in expanding leaves but less than4% in mature tissue. Incorporation of precursor into wall polysaccharideswas insensitive to plant water status in mature leaves, butwas inhibited to less than 50% of well-watered controls in expandingleaves at low water potential. Incorporation of label into cellulose,uronic acid, and neutral sugar fractions was differentiallyaffected by water deficits, with cellulose synthesis apparentlyexhibiting the greatest sensitivity to low water status. Afterrewatering, growth, as well as uptake and incorporation of labelrecovered, although the latter did not attain prestress rates.The results indicate a high sensitivity of wall polysaccharide(particularly cellulose) synthesis to growth-inhibiting waterdeficits.
1 Supported by United States Department of Agriculture, CompetitiveResearch grant GAM 8502539. (Received November 15, 1989; Accepted January 17, 1990) 相似文献
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The degree of photoinhibition of sun and shade grown leaves of grapevine was determined by means of the ratio of variable to maximum chlorophyll (Chl) fluorescence (Fv/Fm) and electron transport measurements. The potential efficiency of photosystem 2 (PS2), Fv/Fm, markedly declined under high irradiance (HI) in shade leaves with less than 10 % of F0 level. In contrast, Fv/Fm ratio declined with about 20 % increase of F0 level in sun leaves. In isolated thylakoids, the rate of whole chain and PS2 activity in HI shade and sun leaves was decreased by about 60 and 40 %, respectively. A smaller inhibition of photosystem 1 (PS1) activity was also observed in both leaf types. In the subsequent dark incubation, fast recovery was observed in both leaf types that reached maximum PS2 efficiencies similar to non-photoinhibited control leaves. The artificial exogenous electron donors DPC, NH2OH, and Mn2+ failed to restore the HI-induced loss of PS2 activity in sun leaves, while DPC and NH2OH were significantly restored in shade leaves. Hence HI in shade leaves inactivates on the donor side of PS2 whereas it does at the acceptor side in sun leaves, respectively. Quantification of the PS2 reaction centre protein D1 and the 33 kDa protein of water splitting complex following HI-treatment of leaves showed pronounced differences between shade and sun leaves. The marked loss of PS2 activity in HI leaves was due to the marked loss of D1 protein of the PS2 reaction centre protein and the 33 kDa protein of the water splitting complex in sun and shade leaves, respectively. 相似文献
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Orasmo GR Oliveira-Collet SA Lapenta AS de Fátima P S Machado M 《Biochemical genetics》2007,45(9-10):663-670
Native polyacrylamide gel electrophoresis (PAGE) was employed to show the highest number of esterase loci and to detect alpha- and beta-esterase polymorphisms in leaf buds of Vitis vinifera cultivars. A total of 16 esterase isozymes were detected in leaf buds from 235 plants including Italia, Rubi, Benitaka, and Brasil cultivars. Biochemical characterization of the grape esterases using ester substrates revealed alpha-, beta-, and alpha/beta-esterases with inhibitor tests distinguishing both carboxylesterases (EST-2, EST-3, EST-5, EST-6, EST-7, EST-8, EST-9, EST-10, and EST-16 isozymes) and acetylesterases (EST-4, EST-11, EST-12, EST-13, EST-14, EST-15 isozymes). No allele variation for alpha-, beta-, and alpha/beta-esterases was detected; however, EST-3 alpha-carboxylesterase was absent in 61.7% of vines, and EST-4 alpha/beta-acetylesterase was absent in one vine of Rubi cv. Null EST-3 carboxylesterase phenotype (61.7%) cannot be explained in this article, but the high genetic polymorphism in four V. vinifera clones is a positive aspect for genetic selection and development of new clones with different characteristics. 相似文献
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Lebon G Wojnarowiez G Holzapfel B Fontaine F Vaillant-Gaveau N Clément C 《Journal of experimental botany》2008,59(10):2565-2578
Sugars play an important role in grapevine flowering. This complex process from inflorescence initiation to fruit maturity takes two growing seasons. Currently, most of the available data concern the involvement of sugars as energy sources during the formation of reproductive structures from initiation of inflorescences during the summer of the first year, until flower opening during the following spring. Sugars devoted to the development of reproductive structures are supplied either by wood reserves or by photosynthesis in leaves or inflorescences, depending on the stage of development. Female meiosis appears to be a key point in the success of flower formation because (i) flowers are vulnerable at this stage and (ii) it corresponds in the whole plant to the transition between reserve mobilization from perennial organs (roots, trunk, and canes) towards efficient leaf photosynthesis. The perturbation of reserve replenishment during the previous year provokes perturbation in the development of inflorescences, whereas altering the photosynthetic sources affects the formation of flowers during the same year. In particular, a lack of sugar availability in flowers at female meiosis caused by various environmental or physiological fluctuations may lead to drastic flower abortion. Apart from energy, sugars also play roles as regulators of gene expression and as signal molecules that may be involved in stress responses. In the future, these two topics should be further investigated in the grapevine considering the sensitivity of flowers to environmental stresses at meiosis. 相似文献
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Enrico Braidot Marco Zancani Elisa Petrussa Carlo Peresson Alberto Bertolini Sonia Patui Francesco Macrì Angelo Vianello 《Plant signaling & behavior》2008,3(9):626-632
Flavonoids are a group of secondary metabolites widely distributed in plants that represent a huge portion of the soluble phenolics present in grapevine (Vitis vinifera L.). These compounds play different physiological roles and are often involved in protection against biotic and abiotic stress. Even if the flavonoid biosynthetic pathways have been largely characterized, the mechanisms of their transport and accumulation in cell wall and vacuole are still not completely understood. This review analyses the known mechanisms of flavonoid uptake and accumulation in grapevine, with reference to the transport models and membrane carrier proteins described in other plant species. The effect of different environmental factors on flavonoid biosynthesis and transporters is also discussed.Key words: ABC proteins, active transport, bilitranslocase, biotic and abiotic stress, flavonoid, secondary metabolites 相似文献