共查询到20条相似文献,搜索用时 45 毫秒
1.
2.
3.
4.
5.
6.
Poly(ADP-ribose) polymerase 1 is inhibited by a histone H2A variant, MacroH2A, and contributes to silencing of the inactive X chromosome 总被引:1,自引:0,他引:1
Nusinow DA Hernández-Muñoz I Fazzio TG Shah GM Kraus WL Panning B 《The Journal of biological chemistry》2007,282(17):12851-12859
Poly(ADP-ribose) polymerase 1 (PARP-1) is a nuclear enzyme that is involved in modulating chromatin structure, regulation of gene expression, and sensing DNA damage. Here, we report that PARP-1 enzymatic activity is inhibited by macroH2A, a vertebrate histone H2A variant that is enriched on facultative heterochromatin. MacroH2A family members have a large C-terminal non-histone domain (NHD) and H2A-like histone domain. MacroH2A1.2 and PARP-1 interact in vivo and in vitro via the NHD. The NHD of each macroH2A family member was sufficient to inhibit PARP-1 enzymatic activity in vitro. The NHD of macroH2A1.2 was a mixed inhibitor of PARP-1 catalytic activity, with affects on both catalytic activity and the substrate binding affinity of PARP-1. Depletion of PARP-1 by RNA interference caused reactivation of a reporter gene on the inactive X chromosome, demonstrating that PARP-1 participates in the maintenance of silencing. These results suggest that one function of macroH2A in gene silencing is to inhibit PARP-1 enzymatic activity, and this may affect PARP-1 association with chromatin. 相似文献
7.
8.
9.
10.
11.
12.
13.
14.
15.
16.
17.
18.
TGF-beta regulation of epithelial cell proliferation. 总被引:4,自引:0,他引:4
H L Moses 《Molecular reproduction and development》1992,32(2):179-184
19.