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1.
Q C Vega 《Journal of industrial microbiology & biotechnology》2000,24(5):359-363
The class consisted of senior molecular biology majors who had previously taken cell biology, microbiology, biochemistry and
molecular biology lecture courses but who had little or no previous lab experience. These students were asked to design and
create an expression vector and purify the expressed protein. This project provides the students with the opportunity to appreciate
interconnections between experiments while learning the necessary techniques. Journal of Industrial Microbiology & Biotechnology (2000) 24, 359–363.
Received 02 April 1999/ Accepted in revised form 10 November 1999 相似文献
2.
Biotechnology is becoming an information-based field. In this article we describe some resources available to instructors,
show how these resources are used in the biotechnology training program, and provide examples of activities used by non-science
majors to increase their understanding of biology. We discuss some of the challenges we have encountered using these tools
in the classroom. Journal of Industrial Microbiology & Biotechnology (2000) 24, 314–318.
Received 08 April 1999/ Accepted in revised form 10 November 1999 相似文献
3.
S D Verhey 《Journal of industrial microbiology & biotechnology》2000,24(5):339-344
This paper describes an integrated laboratory project for intermediate to advanced undergraduate students. The project spans
an entire academic quarter (10 weeks) and involves a series of operations that give students experience with fundamental techniques
in cell biology, molecular biology, biochemistry, genomics, and bioinformatics. In the process, the student learning community
is strengthened, students gain increasing confidence in their abilities in the laboratory, and data are collected toward the
eventual sequencing of a cytoplasmic genome. The culmination of the project is the preparation by students of a paper written
in the format of a particularly accessible online journal. Journal of Industrial Microbiology & Biotechnology (2000) 24, 339–344.
Received 02 April 1999/ Accepted in revised form 22 November 1999 相似文献
4.
J A Slock P M Farrugia T A Horn J A Phillips J T Smedley M J Romanko 《Journal of industrial microbiology & biotechnology》2000,24(5):345-352
Three experiments are described; directional cloning of the luxR gene from the bioluminescent marine bacterium, Vibrio fischeri, directional cloning of the gfpgene from the marine jelly fish, Aequoria victoria, and the construction of a LuxR-GFP fusion protein. Experiments are presented using lux and gfp in an undergraduate biology curriculum. Journal of Industrial Microbiology & Biotechnology (2000) 24, 345–352.
Received 02 April 1999/ Accepted in revised form 19 November 1999 相似文献
5.
J Lin D Panigraphy L B Trinh J Folkman J Shiloach 《Journal of industrial microbiology & biotechnology》2000,24(1):31-35
A pilot-scale production method of recombinant human angiostatin, a 38-kD fragment of plasminogen which has been reported
to have antiangiogenic activity, has been successfully established by expressing the protein in the methylotrophic yeast Pichia pastoris. The secreted protein inhibited cultured endothelial cell proliferation in vitro and Lewis lung carcinoma growth in mice. The fermentation process was carried out using an on-line methanol controller, administering
methanol to the growing culture and keeping its concentration under 2 g L−1. The fermentation lasted 90 h, of which 70 h were growth on methanol. During growth on methanol the culture volume increased
64%, from 7 L to 11.5 L, producing 200 mg angiostatin and 5 kg of biomass. Journal of Industrial Microbiology & Biotechnology (2000) 24, 31–35.
Received 12 May 1999/ Accepted in revised form 06 September 1999 相似文献
6.
P Nanda Devi C Sasikala C V Ramana 《Journal of industrial microbiology & biotechnology》2000,24(3):219-221
Rhodobacter sphaeroides OU5 transformed anthranilate (2 mM) to an indole (0.7 mM) in a light-dependent process. Photobiotransformation was enhanced
by tricarboxylic acid cycle intermediates and the indole formed was identified as 2,3 dihydroxy indole. Journal of Industrial Microbiology & Biotechnology (2000) 24, 219–221.
Received 16 September 1999/ Accepted in revised form 20 December 1999 相似文献
7.
The Biotechnology Certificate Program (BCP) at California State University, Hayward was initiated in 1986 in response to industry
demands for qualified employees in the molecular life sciences. This 9-month post-baccalaureate program includes laboratory
courses in recombinant DNA techniques, protein chemistry, PCR, DNA sequencing, animal cell culture as well as two lecture
courses in molecular biology. Rigorous selection at both entry and exit stages of the program ensures knowledgeable graduates
with a greater than 90% employment placement. Corporate participation has been a cornerstone of the BCP and we anticipate
continued cooperation in the future as the program evolves to meet the expanding needs of the biotechnology industry. Journal of Industrial Microbiology & Biotechnology (2000) 24, 364–366.
Received 19 April 1999/ Accepted in revised form 09 November 1999 相似文献
8.
This collaborative laboratory exercise integrates two upper division laboratory courses (Developmental Biology and Neurobiology)
offered to biology majors at Wake Forest University. The laboratory exercise involves the use of the Xenopus oocyte expression system to study the function of specific membrane receptors and ligand-activated channels. cDNA or mRNA
for receptor proteins is injected into Xenopus oocytes. The oocytes are assayed for expression of receptor proteins and two-electrode voltage clamping is done to determine
whether the expressed proteins are functional in the oocyte system. This series of laboratory exercises is innovative in its
interdisciplinary and collaborative approach to undergraduate teaching, and in its use of sophisticated molecular biological
and physiological techniques in the undergraduate teaching laboratory. Students learn first-hand how these techniques have
been used to achieve a new level of understanding of both development and neurobiology. Journal of Industrial Microbiology & Biotechnology (2000) 24, 353–358.
Received 02 April 1999/ Accepted in revised form 10 November 1999 相似文献
9.
Kogure T Suzuki T Takahashi T Miyamoto D Hidari KI Guo CT Ito T Kawaoka Y Suzuki Y 《Glycoconjugate journal》2006,23(1-2):101-106
We reported previously that the dominant receptors of influenza A and B viruses, and human and murine respiroviruses, were
sialylglycoproteins and gangliosides containing monosialo-lactosamine type I-and II-residues, such as sialic acid-α2-3(6)-Galβ1-3(4)-GlcNAcβ1-. In addition, the Siaα2-3Gal linkage was predominantly recognized by avian and horse influenza viruses, and human parainfluenza
virus type 1 (hPIV-1), whereas the Siaα2-6Gal linkage was mainly recognized by human influenza viruses (Paulson JC in “The
Receptors' [Conn M Ed] 2, 131–219 (1985); Suzuki Y, Prog Lipid Res 33, 429–57 (1994); Ito T, J Virol 73, 6743–51 (2000); Suzuki Y, J Virol 74, 11825–31 (2000); Suzuki T, J. Virol 75, 4604–4613 (2001); Suzuki Y, Biol. Pharm. Bull. 28, 399–408 (2005)). To clarify the distribution of influenza virus receptors on the human bronchial epithelium cell surface,
we investigated a primary culture of normal human bronchial epithelial (NHBE) cells using two types of lectin (MAA and SNA),
which recognize sialyl linkages (α2-3 and α2-6), using fluorescence-activated cell-sorting analysis. The results showed that
both α2-3- and α2-6-linked Sias were expressed on the surface of primary human bronchial epithelial cells. The cells infected
by hPIV-1 bound to MAA, confirming that cells targeted by hPIV-1 have α2-3-linked oligosaccharides. We also compared the ability
of hPIV-1 and human influenza A virus to infect primary human bronchial epithelial cells pre-treated with Siaα2-3Gal-specific
sialidase from Salmonella typhimurium. No difference was observed in the number of sialidase pre-treated and non-treated cells infected with human influenza A
virus, which binds to Siaα2-6Gal-linked oligosaccharides. By contrast, the number of cells infected with hPIV-1 decreased
significantly upon sialidase treatment. Thus, cultured NHBE cells showed both α2-3-linked Sias recognized by hPIV-1 and avian
influenza virus receptors, and α2-6-linked Sias recognized by human influenza virus receptors. 相似文献
10.
B H Chung Y J Choi S H Yoon S Y Lee Y I Lee 《Journal of industrial microbiology & biotechnology》2000,24(2):94-99
Fed-batch cultures were carried out to overproduce human insulin-like growth factor I (IGF-I) in Escherichia coli. The effects of carbon sources (glucose or glycerol) and induction time on cell growth and IGF-I production were investigated
in more detail. Glycerol was a better carbon source than glucose for IGF-I production in fed-batch culture. Induction at the
mid-exponential phase with glycerol as a carbon source in the pH-stat fed-batch culture was optimal for IGF-I production.
Under this condition, 2.8 g L−1 of fusion IGF-I was produced as inclusion bodies. We have also developed downstream processing for preparative scale purification
of IGF-I from the fusion protein produced by the fed-batch culture using glycerol as a carbon source. After the fusion protein
expressed was solubilized in 8 M urea and cleaved with hydroxylamine, the released IGF-I was purified by cation exchange chromatography,
refolding and preparative scale reverse phase HPLC (rp-HPLC) to give recombinant IGF-I of >98% purity. The biological activities
of the purified IGF-I were measured and found to be identical to those of commercial IGF-I. Journal of Industrial Microbiology & Biotechnology (2000) 24, 94–99.
Received 13 January 1999/ Accepted in revised form 02 October 1999 相似文献
11.
Exopolysaccharide (EPS) production was compared among three strains of lactobacilli. Lactobacillus rhamnosus strain 9595M can be classified among the highest EPS-producing strains of lactic acid bacteria reported to date with a maximum
EPS production of 1275 mg L−1. Under controlled pH, no significant differences in the quantity of EPS produced could be detected between carbon source
(glucose or lactose) or fermentation temperature (32 or 37°C). In milk, strains ATCC 9595M and R produced more than 280 mg L−1 EPS whereas strain Type V produced less than 80 mg L−1 EPS. Journal of Industrial Microbiology & Biotechnology (2000) 24, 251–255.
Received 10 September 1999/ Accepted in revised form 22 December 1999 相似文献
12.
This work describes carotenoid pigment production by the red bacterium Brevibacterium linens covering strain diversity, kinetic and analytical aspects. Pigment production of 23 B. linens strains ranged from 0.05 to 0.60 mg pigments L−1 culture, with specific productivity from 0.2 to 0.6 mg pigments per g dry biomass. The pigment production time curve showed
a sigmoid shape, that matched cell growth. HPLC analysis revealed three groups of peaks, possibly non-hydroxylated, mono-
and di-hydroxylated carotenoids. Polar molecules were mainly represented. Journal of Industrial Microbiology & Biotechnology (2000) 24, 64–70.
Received 19 April 1999/ Accepted in revised form 25 September 1999 相似文献
13.
A. W. E. Dilg Giovanna Mincione Klaus Achterhold Olga Iakovleva Matthias Mentler Claudio Luchinat Ivano Bertini F. G. Parak 《Journal of biological inorganic chemistry》1999,4(6):727-741
4 S4]3 + and the reduced [Fe4S4]2 + clusters in the high-potential iron protein I from Ectothiorhodospira halophila were measured in a temperature range from 5 K to 240 K. EPR measurements and 57Fe electron-nuclear double resonance (ENDOR) experiments were carried out with the oxidized protein. In the oxidized state
the cluster has a net spin S = 1/2 and is paramagnetic. As common in [Fe4S4]3 + clusters, the M?ssbauer spectrum was simulated with two species contributing equally to the absorption area: two Fe3 + atoms couple to the “ferric-ferric” pair, and one Fe2 + and one Fe3 + atom give the “ferric-ferrous pair”. For the simulation of the M?ssbauer spectrum, g-values were taken from EPR measurements. A-tensor components were determined by 57Fe ENDOR experiments that turned out to be a necessary source of estimating parameters independently. In order to obtain a
detailed agreement of M?ssbauer and ENDOR data, electronic relaxation has to be taken into account. Relaxing the symmetry
condition in a way that the electric field gradient tensor does not coincide with g- and A-tensors yielded an even better agreement of experimental and theoretical M?ssbauer spectra. Spin-spin and spin-lattice relaxation
times were estimated by pulsed EPR; the former turned out to be the dominating mechanism at T = 5 K. Relaxation times measured by pulsed EPR and obtained from the M?ssbauer fit were compared and yield nearly identical
values. The reduced cluster has one additional electron and has a diamagnetic (S = 0) ground state. All the four irons are indistinguishable in the M?ssbauer spectrum, indicating a mixed-valence state of
Fe2.5 + for each.
Received: 15 February 1999 / Accepted: 31 August 1999 相似文献
14.
For the production of α-D-glucose-1-phosphate (G-1-P), α-1,4-D-glucan phosphorylase from Thermus caldophilus GK24 was partially purified to a specific activity of 13 U mg−1 and an enzyme recovery of 15%. The amount of G-1-P reached maximum (18%) when soluble starch was used as substrate, and the
smallest substrate for G-1-P formation was maltotriose. The structure of purified G-1-P was confirmed by comparison to 13C-NMR data for an authentic sample. In addition to G-1-P, glucose-6-phosphate (12%) was simultaneously produced when 10 mM
maltoheptaose was used as substrate. Journal of Industrial Microbiology & Biotechnology (2000) 24, 89–93.
Received 12 May 1999/ Accepted in revised form 29 August 1999 相似文献
15.
In recent years, it has become clear that the neuronal nicotinic acetylcholine receptor (nAChR) is a valid target in the treatment
of a variety of diseases, including Alzheimer’s disease, anxiety, and nicotine addiction. As with most membrane proteins,
information on the three-dimensional (3D) structure of nAChR is limited to data from electron microscopy, at a resolution
that makes the application of structure-based design approaches to develop specific ligands difficult. Based on a high-resolution
crystal structure of AChBP, homology models of the extracellular domain of the neuronal rat and human nAChR subtypes α4β2
and α7 (the subtypes most abundant in brain) were built, and their stability assessed with molecular dynamics (MD). All models
built showed conformational stability over time, confirming the quality of the starting 3D model. Lipophilicity and electrostatic
potential studies performed on the rat and human α4β2 and α7 nicotinic models were compared to AChBP, revealing the importance
of the hydrophobic aromatic pocket and the critical role of the α-subunit Trp—the homolog of AChBP-Trp 143—for ligand binding.
The models presented provide a valuable framework for the structure-based design of specific α4β2 nAChR subtype ligands aimed
at improving therapeutic and diagnostic applications.
Figure Electrostatic surface potential of the binding site cavity of the neuronal nicotinic acetylcholine receptor (nAChR). Nicotinic
models performed with the MOLCAD program: a rat α7, b rat α4β2, c human α7, d human α4β2. All residues labeled are part of the α7 (a,c) or α4 (b,d) subunit with the exception of Phe 117, which belongs to subunit β2 (d). Violet Very negative, blue negative, yellow neutral, red very positive 相似文献
16.
Increased astaxanthin production by a Phaffia rhodozyma mutant grown on date juice from Yucca fillifera 总被引:1,自引:0,他引:1
J Ramírez M L Nuñez R Valdivia 《Journal of industrial microbiology & biotechnology》2000,24(3):187-190
The wild strain and the astaxanthin-overproducing mutant strain 25–2 of Phaffia rhodozyma were analyzed in order to assess their ability to grow and synthesize astaxanthin in a minimal medium composed of g L−1: KH2PO4 2.0; MgSO4 0.5; CaCl2 0.1; urea 1.0 and supplemented with date juice of Yucca fillifera as a carbon source (yuca medium). The highest astaxanthin production (6170 μg L−1) was obtained at 22.5 g L−1 of reducing sugars. The addition of yeast extract to the yuca medium at concentrations of 0.5–3.0 g L−1 inhibited astaxanthin synthesis. The yuca medium supported a higher production of astaxanthin, 2.5-fold more than that observed
in the YM medium. Journal of Industrial Microbiology & Biotechnology (2000) 24, 187–190.
Received 14 July 1999/ Accepted in revised form 02 December 1999 相似文献
17.
C L Schultz K S Kunert R White 《Journal of industrial microbiology & biotechnology》2000,24(2):113-115
Staphylococcus aureus ATCC 6538 and a clinical isolate of S. aureus from a bacterial keratitis patient were examined for their ability to adhere to etafilcon A, polymacon, silafocon, and pauflufocon
A, B and C contact lenses. Both isolates adhered more to the rigid gas permeable (RGP) materials than to the hydrogel lenses
tested (P < 0.05). S. aureus ATCC 6538 adhered to the etafilcon A material to a greater extent than did the clinical isolate (P < 0.05). there were no statistically significant differences in the recovery of staphylococci from unworn lens materials
when surface area, composition and ionicity were evaluated for either the hydrogel or rgp lenses tested against lenses of
a similar type. however, differences were observed when hydrogel lenses were evaluated against rgp lenses (P < 0.05). these differences may be related to water content. Journal of Industrial Microbiology & Biotechnology (2000) 24, 113–115.
Received 12 August 1999/ Accepted in revised form 02 November 1999 相似文献
18.
Two Saccharomyces cerevisiae strains with different degrees of ethanol tolerance adapted differently to produced ethanol. Adaptation in the less ethanol-tolerant
strain was high and resulted in a reduced formation of ethanol-induced respiratory deficient mutants and an increased ergosterol
content of the cells. Adaptation in the more ethanol-tolerant strain was less pronounced. Journal of Industrial Microbiology & Biotechnology (2000) 24, 75–78.
Received 22 June 1999/ Accepted in revised form 06 October 1999 相似文献
19.
S H de Kock J C du Preez S G Kilian 《Journal of industrial microbiology & biotechnology》2000,24(4):231-236
Aerobic glucose-limited chemostat cultivations were conducted with Saccharomyces cerevisiae strains NRRL Y132, ATCC 4126 and CBS 8066, using a complex medium. At low dilution rates all three strains utilised glucose
oxidatively with high biomass yield coefficients, no ethanol production and very low steady-state residual glucose concentrations
in the culture. Above a threshold dilution rate, respiro-fermentative (oxido-reductive) metabolism commenced, with simultaneous
respiration and fermentation occurring, which is typical of Crabtree-positive yeasts. However, at high dilution rates the
three strains responded differently. At high dilution rates S. cerevisiae CBS 8066 produced 7–8 g ethanol L−1 from 20 g glucose L−1 with concomitant low levels of residual glucose, which increased markedly only close to the wash-out dilution rate. By contrast,
in the respiro-fermentative region both S. cerevisiae ATCC 4126 and NRRL Y132 produced much lower levels of ethanol (3–4 g L−1) than S. cerevisiae CBS 8066, concomitant with very high residual sugar concentrations, which was a significant deviation from Monod kinetics
and appeared to be associated either with high growth rates or with a fermentative (or respiro-fermentative) metabolism. Supplementation
of the cultures with inorganic or organic nutrients failed to improve ethanol production or glucose assimilation. Journal of Industrial Microbiology & Biotechnology (2000) 24, 231–236.
Received 09 August 1999/ Accepted in revised form 18 December 1999 相似文献
20.
N. Rashevsky 《Bulletin of mathematical biology》1969,31(3):605-617
A previous study (Bull. Math. Biophysics,31, 417–427, 1969) on the definitions of stability of equilibria in organismic sets determined byQ relations is continued. An attempt is made to bring this definition into a form as similar as possible to that used in physical
systems determined byF-relations. With examples taken from physics, biology and sociology, it is shown that a definition of equilibria forQ-relational systems similar to the definitions used in physics can be obtained, provided the concept of stable or unstable
structures of a system determined byQ-relations is considered in a probabilistic manner. This offers an illustration of “fuzzy categories,” a notion introduced
by I. Bąianu and M. Marinescu (Bull. Math. Biophysics,30, 625–635, 1968), in their paper on organismic supercategories, which is designed to provide a mathematical formalism for
Rashevsky's theory of Organismic Sets (Bull. Math. Biophysics,29, 389–393, 1967;30, 163–174, 1968;31, 159–198, 1969). A suggestion is made for a method of mapping the abstract discrete space ofQ-relations on a continuum of variables ofF-relations. Problems of polymorphism and metamorphosis, both in biological and social organisms, are discussed in the light
of the theory. 相似文献