电子化温针灸温度场的有限元分析

本文设计了以微型加热装置为核心并辅以电热软膜的电子化温针灸.为了比较电子化温针灸与传统温针灸的温热性质,通过ANSYS软件对传统温针灸及电子化温针灸的温度场进行建模仿真.分析比较得出:在一定的条件下电子化温针灸的温度场与传统温针灸的温度场基本一致.这为电子化温针灸的进一步改进提供了理论依据.     

英美针灸立法

英国针灸立法情况 大多数西方人是从认识针灸开始了解中医药学的。如今,许多西方国家仍把“针灸”作为“中医”的代名词,因而,世界各国对针灸的立法开展得较早,也较普及。但是,针灸和中药在英国曾被认为是彼此分割的两种疗法,专业组织有英国针灸协会和英国中草药注册协会,英国政府一开始曾把针灸单独列为补充医疗的一种,     

英美针灸立法

英国针灸立法情况 大多数西方人是从认识针灸开始了解中医药学的。如今,许多西方国家仍把“针灸”作为“中医”的代名词,因而,世界各国对针灸的立法开展得较早,也较普及。但是,针灸和中药在英国曾被认为是彼此分割的两种疗法,专业组织有英国针灸协会和英国中草药注册协会,英国政府一开始曾把针灸单独列为补充医疗的一种,     

情景教学法与案例教学法在针灸治疗学教学中应用

针灸治疗学是高职院校针灸推拿专业学生必须掌握的一门重要的专业临床学科,针灸治疗学在传统的教学模式下教学效果并不理想。该文基于对针灸治疗学教学的实践,,对情景教学法与案例教学法在针灸治疗学教学中的应用及成效进行了论述,并进行了调查评价总结。     

国外动态

磁电针灸仪日本伊通公司最近研制成功不用针灸针的磁电针灸仪。这种磁电针灸仪有两个通道,一道用于穴位探测,一道用于磁电针灸治疗。磁电针灸电极由一块合成橡胶和一块300高斯的磁铁组成。探测探头对穴位的敏感点能迅速地进行探测,并以声音指示,然后快速地对敏感点进行刺激达到治疗目的。为保证不发生电灼伤,波形设计采用振荡式及正方形和尖峰脉冲混合的脉冲波。探测用脉冲频率为5或20Hz,治疗用脉冲频率在1.5～20Hz,可用开关来选择。治疗方式既可间断,也可连续。以电池为电源,体积240×227×70毫米,重量1.6公斤。这种磁电针灸仪对解除急性和慢性疼痛比用针灸针治     

激光针灸理论与光剂量选择

激光针灸和传统针灸由于物理过程的差异其治疗机理不同。应用生物能量模型定量讨论激光热作用效应,利用几何光学模型解释光作用力的产生及其作用。根据光子中医理论和物理学基本原理,从理论角度探讨激光针灸作机理,并分析影响激光针灸作用的基本因素和最佳治疗光剂量。     

激光针灸治疗仪器的研究历史与现状

我国针灸的历史源远流长,从古代的"砭石"为针,到金属针.到近几十年来的梅花针、电针、水针等,针灸器具也在不断的发展.随着激光技术的成熟与发展,产生了激光针,且激光穴位照射已经广泛应用于生物医学领域.本文综述了针灸器具的发展历史,介绍了激光针灸的作用机理及优点,激光针灸治疗仪器的发展过程及其研究现状、存在的问题,并对其发展进行了展望.     

羊病针灸疗法简介

羊病能否用针灸治疗,由于过去对这方面不够注意,报导较少。实践证明羊病用针灸治疗,具有针感灵敏,见效迅速,疗效持久等优点。 一、羊病常用针灸40穴     

针灸治疗心律失常的研究进展

从针灸治疗心律失常的类型、方法、机制等方面介绍针灸治疗的研究进展,从而凸显针灸在治疗心律失常方面的重要作用,进而提出针药结合在治疗心律失常疾病方面的必要性,从而为祖国医学的发展增添动力。     

生物学研究的一个新领域——国际兽医针灸学术讨论会综述

由中国畜牧兽医学会,中国国际科技会议中心主办的“国际兽医针灸学术讨论会”于5月15日至18日在北京举行,与会专家、学者200多人,其中有来自25个国家和地区的国外代表65人。国际知名专家:中国畜牧兽医学会副主席蒋次升教授、于船教授,国际兽医针灸协会主席,美国的David H.Jagger教授,国际兽医针灸协会科学委员会主席,美国Tuskegee大学教授黄燕卿,日本兽医针灸研究会会长、歧阜大学教授北泽馨等人出席了这次会议。会议交流论文共118篇,集中反映了近年国内国际兽医针灸研究的状况及进展。从中可见近年兽医针灸的研究具有如下特点:     

优化的细菌鞭毛染色技术

优化了实验教材上传统的银染液鞭毛染色方法,用单宁酸和FeCl3做媒染剂,增大单宁酸和FeCl3的质量浓度(并将其配制的溶液分别保存),然后用碱性染料沙黄水溶液[1]、齐氏石炭酸碱性复红染液[1]和稀释10倍吕氏碱性美蓝染液[1],分别对培养好的枯草芽胞杆菌进行染色,得到较粗、清晰的染色结果。     

Spermatogenesis‐associated proteins at different developmental stages of buffalo testicular seminiferous tubules identified by comparative proteomic analysis

The testicular seminiferous tubules contain Sertoli cells and different types of spermatogenic cells. They provide the microenvironment for spermatogenesis, but the precise molecular mechanism of spermatogenesis is still not well known. Here, we have employed tandem mass tag coupled to LC‐MS/MS with the high‐throughput quantitative proteomics technology to explore the protein expression from buffalo testicular seminiferous tubules at three different developmental stages (prepuberty, puberty, and postpuberty). The results show 304 differentially expressed proteins with a ≥2‐fold change, and bioinformatics analysis indicates that 27 of these may be associated with spermatogenesis. Expression patterns of seven selected proteins were verified via Western blot and quantitative RT‐PCR analysis, and further cellular localizations of these proteins by immunohistochemical or immunofluorescence analysis. Taken together, the results provide potential molecular markers of spermatogenesis and provide a rich resource for further studies on male reproduction regulation.     

内皮细胞-血小板血栓体外模型的建立及分析

在内皮细胞培养技术、荧光显微技术和计算机图象处理技术的基础上,建立了内皮细胞－血小板血栓体外动力学模型及其计算机定量分析系统。通过锥板血流模拟装置（Ｃｏｎｅ－ＰｌａｔｅＳｙｓｔｅｍ）结合光－色素法在体外产生血小板血栓,并利用计算机图像处理技术,研究分析了不同剪切应力作用下,内皮细胞的形态变化及血小板吸附情况,定量分析血小板和内皮细胞之间相互作用。该实验模型及其定量分析系统的建立,为深入研究血栓形成和动脉粥样硬化提供了一套在细胞及分子层次作用机理的研究方法,并可进行微量、快速、动态的抗血栓药物的筛选     

Quantitative analysis of activation and inactivation of asymmetry currents in biological membranes,based on a conformational transition model

Summary A basic voltage-dependent conformational transition mechanism is proposed. It comprises one relatively fast conversion between two individual states which are comparatively slowly coupled with a third state. Having introduced voltage as an additional parameter of state, standard methods of thermodynamics and rate theory are employed to describe the equilibrium and kinetic behavior of the system. In particular, a quantitative discussion is given regarding the asymmetrical displacement currents generated by switching on and off a voltage pulse. Effects of temperature pulse duration, and application of a conditioning prepulse are examined. The results provide a comprehensive basis for a quantitative analysis of pertinent experimental work. The so far presented measuring data can indeed be very well described along these lines.     

Metabolic control analysis as a mechanism that conserves genetic variance during advanced cycle breeding

The recycling of elite inbreds (i.e., advanced cycle breeding) has led to significant genetic gains but also to a narrow gene pool in plant breeding programs. Sustained yield improvements in many crops have suggested that genetic variance is not depleted at a rate predicted by an additive genetic model. Unlike the additive model in classical quantitative genetic theory, metabolic control analysis relates the variation in a biochemical process with the genetic variation in a quantitative trait. Our objective was to determine whether metabolic control analysis is a mechanism that slows the decrease in genetic variance during advanced cycle breeding. Three cycles of advanced cycle breeding were simulated with 10, 50, or 100 quantitative trait loci (QTL) controlling a trait. In metabolic control analysis, these QTL coded for enzymes involved in a linear metabolic pathway that converted a substrate into a product. In the absence of selection, both the additive model and the metabolic control analysis model led to about a 50% reduction in genetic variance from cycle to cycle. With selection, the additive model led to a 50–58% reduction in genetic variance, but the metabolic control analysis model generally led to only a 12–54% reduction. We suggest selection in a metabolic control analysis model as a mechanism that slows the decrease in genetic variance during advanced cycle breeding. This conservation of genetic variance would allow breeders to achieve genetic gains for a longer period than expected under the additive model.Communicated by H.C. Becker     

Urea biosynthesis using liver slices

A practical scheme for students is given, to enable them to investigate some aspects of the mechanism by which urea is synthesized in the liver. The tissue-slice technique is discussed, and methods for the quantitative analysis of metabolites are presented.     

Cytoplasmic Dynamics Reveals Two Modes of Nucleoid-Dependent Mobility

It has been proposed that forces resulting from the physical exclusion of macromolecules from the bacterial nucleoid play a central role in organizing the bacterial cell, yet this proposal has not been quantitatively tested. To investigate this hypothesis, we mapped the generic motion of large protein complexes in the bacterial cytoplasm through quantitative analysis of thousands of complete cell-cycle trajectories of fluorescently tagged ectopic MS2-mRNA complexes. We find the motion of these complexes in the cytoplasm is strongly dependent on their spatial position along the long axis of the cell, and that their dynamics are consistent with a quantitative model that requires only nucleoid exclusion and membrane confinement. This analysis also reveals that the nucleoid increases the mobility of MS2-mRNA complexes, resulting in a fourfold increase in diffusion coefficients between regions of the lowest and highest nucleoid density. These data provide strong quantitative support for two modes of nucleoid action: the widely accepted mechanism of nucleoid exclusion in organizing the cell and a newly proposed mode, in which the nucleoid facilitates rapid motion throughout the cytoplasm.     

Refinement of the fluid-mosaic model of membrane structure

Certain molecular packing criteria previously employed in a quantitative analysis of lipid micelles and bilayers are here extended to biological membranes. The inclusion of both thermodynamic and packing considerations point to a highly complex self-assembly mechanism in which the organization of lipids and proteins is highly coupled, with far reaching consequences as regards the structure and function of biological membranes.