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1.
In two Japanese cicadas, Cryptotympana facialis and Graptopsaltria nigrofuscata , with different habitat distributions, fully developed embryos hatch in response to high humidity due to rainfall. Despite the advantage of hatching on rainy days, this trait burdens embryos with an extra period of desiccation until the unpredictable advent of rain. We compared the ability of the fully developed embryos of these cicadas to endure periods of low humidity. Eggs were exposed to a combination of different humidities (43% and 75% relative humidity, RH) and durations (0–15 days), and then transferred to an environment with 100% RH to stimulate hatching. In both species, total hatching rates decreased as duration increased, although there was no significant effect of humidity. In C. facialis , a considerable proportion of the eggs hatched during the desiccation period, and the hatching rate was higher at 75% RH than at 43% RH. After transfer to 100% RH, most hatching occurred within a day regardless of the desiccation level. In G. nigrofuscata , no nymphs hatched during the desiccation periods. However, more eggs required more than a day after transfer to 100% RH to hatch after desiccation at 43% RH than at 75% RH. Consequently, the overall proportion of timely hatching of eggs (eggs hatching within a day of moisture supply) was higher after desiccation at 43% RH in C. facialis , but it was higher after desiccation at 75% RH in G. nigrofuscata . These different physiological responses of the two species may reflect adaptation to habitat dryness. 相似文献
2.
Does anti‐parasitoid defense explain host‐plant selection by a polyphagous caterpillar? 总被引:6,自引:0,他引:6
While studies of tri-trophic interactions have uncovered a variety of mechanisms influencing the dietary specialization of insect herbivores, such studies have neglected host-plant selection by generalists. Here, we report an initial investigation on how host-plant quality and a tachinid parasitoid interact to affect the survival and host-plant selection by a polyphagous herbivore. This herbivore, Grammia geneura (Strecker) (Lepidoptera: Arctiidae), is a food-mixing caterpillar that feeds preferentially on forbs. A previous study suggested that G. geneura might eat certain host species for reasons other than benefits of physiological utilization. We hypothesized that host-plant mediated defenses could act against parasitoids, the major mortality agents of late instar G. geneura . Field observations indicated that caterpillars sometimes survived an attack by the parasitoid Exorista mella Walker (Diptera: Tachinidae) in nature. Laboratory experiments showed that the survival of parasitized caterpillars increased on acceptable but nutritionally inferior host-plant species, indicating that anti-parasitoid defense may explain host-plant selection in this dietary generalist. We found no indication that host-plant selection changed according to the parasitism status of individual caterpillars. 相似文献
3.
The availability of water is recognized as the most important determinant of the distribution and activity of terrestrial organisms within the maritime Antarctic. Within this environment, arthropods may be challenged by drought stress during both the austral summer, due to increased temperature, wind, insolation, and extended periods of reduced precipitation, and the winter, as a result of vapor pressure gradients between the surrounding icy environment and the body fluids. The purpose of the present study was to assess the desiccation tolerance of the Antarctic springtail, Cryptopygus antarcticus, under ecologically-relevant conditions characteristic of both summer and winter along the Antarctic Peninsula. In addition, this study examined the physiological changes and effects of mild drought acclimation on the subsequent desiccation tolerance of C. antarcticus. The collembolans possessed little resistance to water loss under dry air, as the rate of water loss was >20% h(-1) at 0% relative humidity (RH) and 4 degrees C. Even under ecologically-relevant desiccating conditions, the springtails lost water at all relative humidities below saturation (100% RH). However, slow dehydration at high RH dramatically increased the desiccation tolerance of C. antarcticus, as the springtails tolerated a greater loss of body water. Relative to animals maintained at 100% RH, a mild drought acclimation at 98.2% RH significantly increased subsequent desiccation tolerance. Drought acclimation was accompanied by the synthesis and accumulation of several sugars and polyols that could function to stabilize membranes and proteins during dehydration. Drought acclimation may permit C. antarcticus to maintain activity and thereby allow sufficient time to utilize behavioral strategies to reduce water loss during periods of reduced moisture availability. The springtails were also susceptible to desiccation at subzero temperatures in equilibrium with the vapor pressure of ice; they lost approximately 40% of their total body water over 28 d when cooled to -3.0 degrees C. The concentration of solutes in the remaining body fluids as a result of dehydration, together with the synthesis of several osmolytes, dramatically increased the body fluid osmotic pressure. This increase corresponded to a depression of the melting point to approximately -2.2 degrees C, and may therefore allow C. antarcticus to survive much of the Antarctic winter in a cryoprotectively dehydrated state. 相似文献
4.
Lyngbya major (a wall alga), survived throughout year, maximally to >80 % at atmospheric temperature (AT) of 17-36 degrees C and relative humidity (RH) 60-100 % in rainy and spring seasons, but the survival was 43-64 % in winter when AT decreased to 5 degrees C and RH was 65-98 %, and 15-23 % in summer when AT reached 48 degrees C and RH was 23-60 %. All soil algae (Lyngbya birgei, Aphanothece pallida, Gloeocapsa atrata, Oscillatoria subbrevis, O. animalis) survived >90 % in rainy season when soil moisture content (SMC) was 89-100 %. Lowering of SMC to a minimum of 55 % in spring and 39 % in winter led L. birgei, O. subbrevis and O. animalis to survive from 75, 66, and 65 %, respectively, in spring and 12, 14, and 20 % in winter, and A. pallida and G. atrata not at all in both seasons. All soil algae did not survive in summer when SMC was 12-30 %. Myxosarcina burmensis survived only in rainy and spring seasons when pond water temperature (PWT) was 19-25 degrees C and 18-26 degrees C, respectively, and not in winter and summer when PWT was 2-14 degrees C and 25-36 degrees C, respectively. L. major and A. pallida survived almost equally well under both submerged and air-exposed conditions for 15 d but less if submerged for more time than air-exposed on moist soil surface, while L. birgei, G. atrata, O. subbrevis, and O. animalis survived submergence in liquid medium better and longer than air-exposure on moist soil surface. Pond alga M. burmensis survived submergence better than air-exposure, true to its aquatic habitat. All algae survived less and died without forming any resistant cells when exposed to physical and physiological water stress (imposed by growing them on highly agarized media or in salinized liquid media), light stress (at 0, 2 and 10 mumol m(-2) s(-1) light intensity) or following UV shock (0.96-3.84 kJ/m(2)). A. pallida and G. atrata cells did not divide on 8 % agarized solid media, in >/=0.3 mol/L salinized liquid media, and in darkness. The presence of sheath over L. major and L. birgei filament cells and mucilage cover over A. pallida and G. atrata cells protect them against physical desiccation to some extent but not against UV shock. 相似文献
5.
Renae J. Brodie 《Invertebrate Biology》2005,124(3):265-272
Abstract. The terrestrial hermit crab Coenobita compressus H. M ilne E dwards undergoes larval development in the sea and then moves to land as a megalopa, where it metamorphoses and remains for the rest of its life. As a small organism (generally <3 mg), in a body adapted for pelagic life, the megalopa must avoid desiccating in air to make a successful sea-to-land transition. In this study, I measured rates of water loss in 1 to 26-day-old megalopae without mollusk shells to determine if there is an improvement in desiccation resistance with age. I also exposed 26-day-old megalopae with or without shells to different relative-humidity conditions for 1 h to determine if shells worn by megalopae allow them to function in air that is not fully saturated. Megalopae without shells did not survive exposure to a relative humidity (RH) of less than 99%, while those with shells survived 52% RH. Older megalopae lost water more slowly than younger ones. However, the amount of body water explained more of the variation in water-loss rate than age; individuals with smaller body-water masses showed lower rates of absolute water loss. Though megalopae of C. compressus become less water permeable as they approach metamorphosis on land, most of their ability to avoid desiccation comes from the shell. Shell-wearing can be considered a pre-adaptation to a terrestrial life-style because shell-wearing behavior predates land invasion in hermit crabs, and desiccation-proofing in air is a novel function for shells. 相似文献
6.
Spatial and temporal variation in the parasitoid assemblage of an exophytic polyphagous caterpillar 总被引:1,自引:0,他引:1
Abstract 1. Over 3400 larvae of the polyphagous ground dwelling arctiid Grammia geneura were sampled and reared over seven generations in order to characterise its parasitoid assemblage and examine how and why this assemblage varies over time and space at a variety of scales.
2. The total parasitoid assemblage of 14 species was dominated both in diversity and frequency by relatively polyphagous tachinid flies.
3. Both the composition of the parasitoid assemblage and frequency of parasitism varied strikingly among and within sampling sites, seasons, and years.
4. Overall rates of parasitism increased consistently over the duration of caterpillar development.
5. Within sampling sites, parasitism rates were non-random with respect to habitat structure and caterpillar behaviour for the most abundant parasitoid species.
6. The large variability in parasitoid assemblage structure over space and time in this system may be a function of local host population abundance, habitat-specific parasitism, and indirect interactions between G. geneura and other Macrolepidoptera through shared oligophagous and polyphagous parasitoids. 相似文献
2. The total parasitoid assemblage of 14 species was dominated both in diversity and frequency by relatively polyphagous tachinid flies.
3. Both the composition of the parasitoid assemblage and frequency of parasitism varied strikingly among and within sampling sites, seasons, and years.
4. Overall rates of parasitism increased consistently over the duration of caterpillar development.
5. Within sampling sites, parasitism rates were non-random with respect to habitat structure and caterpillar behaviour for the most abundant parasitoid species.
6. The large variability in parasitoid assemblage structure over space and time in this system may be a function of local host population abundance, habitat-specific parasitism, and indirect interactions between G. geneura and other Macrolepidoptera through shared oligophagous and polyphagous parasitoids. 相似文献
7.
Large populations of Pratylenchus thornei, a winter pest of cereals, legumes, and potatoes in the northern Negev region of Israel, survive 7-8 months of summer drought and return to full activity at the beginning of the rainy season. To demonstrate that it survives the summer in an anhydrobiotic state, all developmental stages of P. thornei were exposed to gradually reduced relative humidity (RH) using glycerin water solutions. At 97.7% RH the nematodes were coiled and able to survive exposure to 0% RH. About 40% of artificially desiccated nematodes could be reactivated by gradually increasing the humidity to the final water environment. Desiccated nematodes could withstand temperatures up to 40 C. Reactivated individuals showed intestines apparently devoid of reserve materials. Only 3% survived three cycles of desiccation and reactivation. P. thornei reactivated after anhydrobiosis multiplied twice as much within Vicia sativa roots as did fresh nematodes. 相似文献
8.
The survival of desiccation by J4 Orrina phyllobia was examined at controlled relative humidities. When nematodes were transferred from water to air at 10% relative humidity (rh), 80% died within 30 minutes. When nematodes were transferred from water to air with rh at 70% or greater for ca. 15 minutes prior to being transferred to 10% rh, more than 90% of them survived desiccation. This phenomenon is referred to as preconditioning and occurred at much faster rates (2-30 minutes) than has been observed for other nematode species (24 hours). Differences in preconditioning rates may be due to technique-dependent variations in boundary layer resistance around nematodes during desiccation. 相似文献
9.
Desiccation tolerance of recalcitrant Theobroma cacao embryonic axes: the optimal drying rate and its physiological basis 总被引:7,自引:0,他引:7
Recalcitrant seed axes were reported to survive to lower water contents under fast-drying conditions. The present study was to examine the hypothesis that drying rate and dehydration duration could interact to determine desiccation tolerance through different physico-chemical mechanisms. The effect of drying rate on desiccation tolerance of Theobroma cacao seed axes at 16 degrees C was examined. Rapid-drying at low relative humidity (RH) and slow-drying at high RH were more harmful to cocoa axes, because electrolyte leakage began to increase and axis viability began to decrease at high water contents. Maximum desiccation tolerance was observed with intermediate drying rates at RH between 88% and 91%, indicating the existence of an optimal drying rate or optimal desiccation duration. This maximum level of desiccation tolerance for cocoa axes (corresponding to a critical water potential of -9 MPa) was also detected using the equilibration method, in which axes were dehydrated over a series of salt solutions or glycerol solutions until the equilibrium. These data confirmed that the physiological basis of the optimal drying rate is related to both mechanical stress during desiccation and the length of desiccation duration during which deleterious reactions may occur. The optimal drying rate represents a situation where combined damages from mechanical and metabolic stresses become minimal. 相似文献
10.
A desiccation protocol was developed to evaluate the effect of different levels of desiccation on germination and plantlet
regeneration of black spruce somatic embryos. Large desiccation chambers (80 l) with four liters of saturated salt solutions
provided constant relative humidities (RH) of 63, 79, 88, and 97% (± 2%). Under these conditions, an embryo mass of 10 mg
always dried fast even at 97% RH. In contrast, an embryo mass of 80 mg generated different kinetics of water loss, from fast
drying at 63% RH to slow drying at 97% RH. Drying rates similar to those obtained with 80 mg embryos were also generated by
combining 40 mg embryos with 40 mg water. The effects of drying rate and embryo MC on germination rate, root elongation, and
plantlet regeneration were examined. A fast drying rate to 4–5% embryo MC, obtained under 63% RH, was detrimental to germination
and plantlet development. However slower drying rates, obtained under 79–97% RH and generating 7–19% MC in the embryos, gave
developmental responses similar to the control. Synchronization of root emergence was improved only for embryos desiccated
to approx. 16% MC under 97% RH. The optimal desiccation protocol using large desiccation chamber at 97% RH and a constant
embryo mass of 40 mg embryos plus 40 mg water was applied to five genotypes of black spruce. For all genotypes, desiccated
embryos gave plantlet regeneration rates similar to the control undesiccated embryos.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
11.
Colletotrichum truncatum conidia produced from a one week-old culture in a liquid semi-defined medium with a C:N ratio of 5:1 were more tolerant of desiccation than those harvested from two or three week-old cultures. Conidia washed with 20% (w/v) sucrose germinated better than unwashed conidia or those washed in 10% (w/v) sucrose, 10 and 20% (w/v) glucose or fructose, 0.1% (w/v) soluble starch, 0.9% (w/v) NaCl or deionized water. Washing with sucrose (20% w/v) also resulted in significantly longer germ tubes than those produced by unwashed conidia or conidia washed with deionized water or NaCl (0.9% w/v). Conidia washed twice in sucrose showed greater desiccation tolerance during storage at 15% relative humidity (RH) and 15°C than at 30% RH and 15 or 25°C or at 15% RH and 25, 5 or -10°C. 相似文献
12.
Influence of temperature and humidity on the survival of Monilinia fructicola conidia on stone fruits and inert surfaces
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M. Bernat J. Segarra J.A. Navas‐Cortés C. Casals R. Torres N. Teixidó J. Usall 《The Annals of applied biology》2018,173(1):63-70
The survival of the fungus Monilinia fructicola on fruit and inert surfaces at different temperatures (range: 0–30°C) and relative humidity (RH) (range: 60–100%) was investigated. M. fructicola conidia survived better on fruit than on inert surfaces. The viability reduction rate at 20°C and 60% RH was 1.2 and 5.8 days?1 on fruit and inert surfaces, respectively. Overall, on fruit surfaces, conidia viability was reduced at high temperatures and was longer at higher RH than at lower RH; in contrast, on inert surfaces, conidia viability was longer at only low temperatures. On fruit surfaces, at 0°C and 100% RH, conidia survived up to 35 days, and at 30°C and 60% RH, conidia survived up to 7 days. However, on inert surfaces at 20°C and 30°C, conidia lost their viability after 48 and 24 h, respectively. These results suggest that M. fructicola can remain viable in cold rooms for over 30 days on fruit surfaces or over 25 days on inert surfaces. Furthermore, under the orchard conditions during the growing season, conidia may remain viable for only 2–3 days on immature fruit surfaces before conidia will be unable to penetrate the host. 相似文献
13.
Aiqing Li Joshua B. Benoit Giancarlo Lopez‐Martinez Michael A. Elnitsky Richard E. Lee Jr David L. Denlinger 《Proteomics》2009,9(10):2788-2798
Desiccation presents a major challenge for the Antarctic midge, Belgica antarctica. In this study, we use proteomic profiling to evaluate protein changes in the larvae elicited by dehydration and rehydration. Larvae were desiccated at 75% relative humidity (RH) for 12 h to achieve a body water loss of 35%, approximately half of the water that can be lost before the larvae succumb to dehydration. To evaluate the rehydration response, larvae were first desiccated, then rehydrated for 6 h at 100% RH and then in water for 6 h. Controls were held continuously at 100% RH. Protein analysis was performed using 2‐DE and nanoscale capillary LC/MS/MS. Twenty‐four identified proteins changed in abundance in response to desiccation: 16 were more abundant and 8 were less abundant; 84% of these proteins were contractile or cytoskeletal proteins. Thirteen rehydration‐regulated proteins were identified: 8 were more abundant and 5 were less abundant, and 69% of these proteins were also contractile or cytoskeletal proteins. Additional proteins responsive to desiccation and rehydration were involved in functions including stress responses, energy metabolism, protein synthesis, glucogenesis and membrane transport. We conclude that the major protein responses elicited by both desiccation and rehydration are linked to body contraction and cytoskeleton rearrangements. 相似文献
14.
Dry artificial seeds and desiccation tolerance induction in microspore-derived embryos of broccoli 总被引:2,自引:0,他引:2
Y. Takahata D. C. W. Brown W. A. Keller N. Kaizuma 《Plant Cell, Tissue and Organ Culture》1993,35(2):121-129
Desiccation tolerance of broccoli microspore-derived embryos was induced by exogenous application of abscisic acid (ABA). Embryos, which were desiccated to about 10% water content, were estimated for viability after rehydration. Survival was dependent on the ABA concentration and the development stage of embryo, but not on the length of exposure period to ABA or genotype. Cotyledonary stage embryos acquired the highest desiccation tolerance when treated with 1×10-4M ABA. Under this condition, on average 27–48% of the desiccated embryos could convert into plants. Embryos treated with 1×10-6M ABA or no ABA or earlier development-staged embryos, such as globular and heart stages, lost viability after desiccation. A one day exposure to ABA had the similar effect on the induction of desiccation tolerance as a 7-day treatment. The dried embryos maintained their ability of plant conversion after three months of storage under room conditions. The plants derived from the desiccated embryos were not different in the morphology or ploidy level from those from non-desiccated ones.Abbreviations ABA
abscisic acid
- RH
relative humidity 相似文献
15.
Proctor MC 《Annals of botany》2003,91(6):717-727
Chlorophyll-fluorescence and infrared gas analyser measurements show saturation of photosynthetic electron flow and CO(2) uptake at generally lower irradiances in Hymenophyllum tunbrigense than in H. wilsonii, but with wide variation in both species (63-189 micromol m(-2) s(-1) PPFD in H. tunbrigense, 129-552 micro mol m(-2) s(-1) PPFD in H. wilsonii), probably related to both site and season. Non-photochemical quenching (at 400 micromol m(-2) s(-1) PPFD) ranged from 2.1 to 8.1, with no significant difference between the species. Pressure-volume curves from thermocouple-psychrometer measurements give full-turgor osmotic potentials of approx. -1.4 MPa in both species, and indicate low apoplast fractions and high cell-wall elastic moduli. Leaves of H. tunbrigense recovered within 24 h from up to 7 d desiccation at water potentials ranging from -40 MPa (74 % relative humidity, RH) to -220 MPa (20 % RH); after 15 or 30 d, desiccation recovery was slower and less complete, and leaves were severely damaged at the highest and lowest humidities. Hymenophyllum wilsonii recovered well from up to 30 d desiccation at -114 and -220 MPa, but at -40 MPa it showed signs of damage after 15 d, and was severely damaged or killed after 30 d. Results are discussed in relation to the ecological and geographical distributions of the two species, and to the adaptive strategies of filmy ferns in general. 相似文献
16.
Rates of Drying and Survival of Rhizobium meliloti Strains During Storage at Different Relative Humidities 总被引:5,自引:2,他引:3
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An investigation was made of the survival of six strains of Rhizobium meliloti filtered on membrane filters and held in atmospheres of controlled relative humidities (RH) of from 0 to 100% at 30°C in the presence of air. The rate of water loss in the desiccator was determined by the humidity-controlling solution used. Drying was accelerated by a mild evacuation of the desiccator during the drying step. Survival rates of R. meliloti strains were much higher after slow drying to 0% RH than immediately after rapid drying. Fast drying (drying period less than 3.4 h) was shown to adversely affect the tolerance to storage at all RH values tested (no survival after 2 to 5 days of storage). When survival during storage was measurable (after slow drying), the optimum RH values for storage were 43% for strains A145 and Wu498, 22 to 43% for strains RCR2011, Wu499, and Ar16, and 83% for strain RCR2004. The most favorable drying periods were 8, 9.2, 14.2, and 50.1 h for the subsequent storage of strain RCR2011 at RH values of 0, 22, 43, and 83%, respectively. The damaging effects of rapid drying on the tolerance of strain RCR2011 to storage at different RH values could be prevented either by rehydration and subsequent slow redrying or incomplete rapid drying followed by slow drying. It is suggested that R. meliloti strains are susceptible to desiccation stresses. However, the quantitative differences among strains appear to be large enough to permit selection with regard to tolerance to desiccation and storage in dried states. 相似文献
17.
Hartmann T Theuring C Beuerle T Bernays EA Singer MS 《Insect biochemistry and molecular biology》2005,35(10):1083-1099
The polyphagous arctiid Grammia geneura appears well adapted to utilize for its protection plant pyrrolizidine alkaloids of almost all known structural types. Plant-acquired alkaloids that are maintained through all life-stages include various classes of macrocyclic diesters (typically occurring in the Asteraceae tribe Senecioneae and Fabaceae), macrocyclic triesters (Apocynaceae) and open-chain esters of the lycopsamine type (Asteraceae tribe Eupatorieae, Boraginaceae and Apocynaceae). As in other arctiids, all sequestered and processed pyrrolizidine alkaloids are maintained as non-toxic N-oxides. The only type of pyrrolizidine alkaloids that is neither sequestered nor metabolized are the pro-toxic otonecine-derivatives, e.g. the senecionine analog senkirkine that cannot be detoxified by N-oxidation. In its sequestration behavior, G. geneura resembles the previously studied highly polyphagous Estigmene acrea. Both arctiids are adapted to exploit pyrrolizidine alkaloid-containing plants as "drug sources". However, unlike E. acrea, G. geneura is not known to synthesize the pyrrolizidine-derived male courtship pheromone, hydroxydanaidal, and differs distinctly in its metabolic processing of the plant-acquired alkaloids. Necine bases obtained from plant acquired pyrrolizidine alkaloids are re-esterified yielding two distinct classes of insect-specific ester alkaloids, the creatonotines, also present in E. acrea, and the callimorphines, missing in E. acrea. The creatonotines are preferentially found in pupae; in adults they are largely replaced by the callimorphines. Before eclosion the creatonotines are apparently converted into the callimorphines by trans-esterification. Open-chain ester alkaloids such as the platynecine ester sarracine and the orchid alkaloid phalaenopsine, that do not possess the unique necic acid moiety of the lycopsamine type, are sequestered by larvae but they need to be converted into the respective creatonotines and callimorphines by trans-esterification in order to be transferred to the adult stage. In the case of the orchid alkaloids, evidence is presented that during this processing the necine base (trachelanthamidine) is converted into its 7-(R)-hydroxy derivative (turneforcidine), indicating the ability of G. geneura to introduce a hydroxyl group at C-7 of a necine base. The creatonotines and callimorphines display a striking similarity to plant necine monoesters of the lycopsamine type to which G. geneura is well adapted. The possible function of insect-specific trans-esterification in the acquisition of necine bases derived from plant acquired alkaloids, especially from those that cannot be maintained through all life-stages, is discussed. 相似文献
18.
Single amino acid changes in domain II of Bacillus thuringiensis CryIAb delta-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles. 总被引:3,自引:0,他引:3
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F Rajamohan E Alcantara M K Lee X J Chen A Curtiss D H Dean 《Journal of bacteriology》1995,177(9):2276-2282
Deletion of amino acid residues 370 to 375 (D2) and single alanine substitutions between residues 371 and 375 (FNIGI) of lepidopteran-active Bacillus thuringiensis CryIAb delta-endotoxin were constructed by site-directed mutagenesis techniques. All mutants, except that with the I-to-A change at position 373 (I373A), produced delta-endotoxin as CryIAb and were stable upon activation either by Manduca sexta gut enzymes or by trypsin. Mutants D2, F371A, and G374A lost most of the toxicity (400 times less) for M. sexta larvae, whereas N372A and I375A were only 2 times less toxic than CryIAb. The results of homologous and heterologous competition binding assays to M. sexta midgut brush border membrane vesicles (BBMV) revealed that the binding curves for all mutant toxins were similar to those for the wild-type toxin. However, a significant difference in irreversible binding was observed between the toxic (CryIAb, N372A, and I375A) and less-toxic (D2, F371A, and G374A) proteins. Only 20 to 25% of bound, radiolabeled CryIAb, N372A, and I375A toxins was dissociated from BBMV, whereas about 50 to 55% of the less-toxic mutants, D2, F371A, and G374A, was dissociated from their binding sites by the addition of excess nonlabeled ligand. Voltage clamping experiments provided further evidence that the insecticidal property (inhibition of short-circuit current across the M. sexta midgut) was directly correlated to irreversible interaction of the toxin with the BBMV. We have also shown that CryIAb and mutant toxins recognize 210- and 120-kDa peptides in ligand blotting. Our results imply that mutations in residues 370 to 375 of domain II of CrylAb do not affect overall binding but do affect the irreversible association of the toxin to the midgut columnar epithelial cells of M. sexta. 相似文献
19.
I. D. Marsden 《Hydrobiologia》1991,223(1):149-158
Total gill area and gill distribution were measured for the sandhopper Talorchestia quoyana (Milne-Edwards) and the beach flea Transorchestia chiliensis (Milne-Edwards). For both species the gill structure and proportional area contributed by individual gills was similar. Gill 6 (G6) was the largest, providing 36% of the gill area in Tal. quoyana and 30% in Tr. chiliensis. The gill area/total dry weight relationships were similar, Y = 1.3 X0.79 for Tal. quoyana and 1.4 X0.78 for Tr. chiliensis. Small, medium and large amphipods survived >24 h in aerial conditions close to 100% RH at 15 °C. Rates of water loss in desiccating conditions increased with decreasing RH. Lethal water loss exceeded 30% weight loss for both species. Rate of water loss, (R) mg water loss. mg wet wt tissue. h–1 exposed to 75% RH for Tr. chiliensis was 0.21, resulting in total mortality within 2 h. Medium Tal. quoyana were the most resistant group surviving 4 h exposure to 75% RH with R = 0.08. Differences in desiccation tolerances of the two amphipods are not explained by body water content, gill area relationships or the larger maximal size of T. quoyana. Results were combined with those from other talitrids to examine the relationship between gill area, water content, desiccation habitat and oxygen consumption in aerial and aquatic conditions. There were no consistent relationship between gill area, O2 uptake and desiccation resistance. Amphipods show compensatory respiratory adaptation with individuals from all habitats, showing similar rates of oxygen uptake, either in air or in water, whichever was their most usual respiratory medium. Q10 values close to 2.0 were found in all ecomorphological groups. Sandhoppers, including Tal. quoyana, are best able to survive terrestrial conditions associated with a low humidity environment. It is concluded that the water loss characteristics of Tr. chiliensis limit its distribution on sand beaches to areas of high relative humidities. 相似文献
20.
Habtamu Giday Katrine H. Kjaer Dimitrios Fanourakis Carl-Otto Ottosen 《Journal of plant physiology》2013
Stomata formed at high relative air humidity (RH) close less as leaf dries; an effect that varies depending on the genotype. We here quantified the contribution of each stomatal response characteristic to the higher water loss of high RH-grown plants, and assessed the relationship between response characteristics and intraspecific variation in stomatal size. Stomatal size (length multiplied by width), density and responsiveness to desiccation, as well as pore dimensions were analyzed in ten rose cultivars grown at moderate (60%) or high (85%) RH. Leaf morphological components and transpiration at growth conditions were also assessed. High growth RH resulted in thinner (11%) leaves with larger area. A strong positive genetic correlation of daytime and nighttime transpiration at either RH was observed. Stomatal size determined pore area (r = 0.7) and varied by a factor of two, as a result of proportional changes in length and width. Size and density of stomata were not related. Following desiccation, high RH resulted in a significantly lower (6–19%) decline of transpiration in three cultivars, whereas the relative water content (RWC) of high RH-expanded leaflets was lower (29–297%) in seven cultivars. The lower RWC of these leaflets was caused by (a) higher (33–72%) stable transpiration and/or (b) lower (12–143%) RWC at which this stable transpiration occurred, depending on the cultivar. Stomatal size was significantly correlated with both characteristics (r = 0.5 and -0.7, respectively). These results indicate that stomatal size explains much of the intraspecific variation in the regulation of transpiration upon water deprivation on rose. 相似文献