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1.
Paul R. Burton 《Cell and tissue research》1968,87(2):226-248
Summary Sperm of the frog lung-fluke, Haematoloechus medioplexus, were treated in various ways and their microtubules and axial units were subsequently studied in sectioned and negatively-stained material. Microtubules and axial units were generally unaffected by exposure to colchicine, cold, and KCl, although with KCl certain lateral projections from doublet tubule A appeared more prominent in negatively-stained preparations. Both mercaptoethanol and urea have a dissociative effect on doublet tubules and microtubules, with doublet tubules being the more sensitive. Pepsin-HCl initially digests the dense region associated with the A tubule of a doublet pair and the core of the axial unit. Microtubules and B tubules of doublet units are later digested; in microtubules, there appears to be a proteinaceous material in the lucent central region which is digested before disappearance of the wall of the microtubule. Further evidence is presented indicating that the characteristically helical wall of the microtubules is made up of spherical subunits about 50 Å in diameter, with about 8 subunits in one turn of the helix. Under certain conditions, the helical structure may be altered to form a wall comprised of longitudinal filaments. It is emphasized that not all microtubules are structurally and chemically equivalent, and it follows that all microtubules do not share a common function.This research was supported by U.S. Public Health Service Grant AI-06448 and an institutional grant from the American Cancer Society. 相似文献
2.
Thomas A. Keil 《Tissue & cell》1982,14(3):451-462
Antennal olfactory hairs of Antheraea polyphemus were investigated by means of transmission electron microscopy. Adequate preservation of dendrites and extracellular pore tubules is obtained by mechanical opening of the hair lumen and subsequent chemical fixation. The dendritic membrane has a cell coat. The dendrites contain microfilamentous structures in addition to their cytoplasmatic microtubules. The extracellular pore tubules traverse the hair cuticle and reach into the hair lumen for maximally 350 nm. Their diameter varies between 20 and 40 nm, depending on the preparation method. They consist of an electron-dense wall and an electron-lucent core. The wall has a helical substructure and is covered with a fuzzy coat. Contacts of pore tubules and dendritic membranes occur wherever dendrites are near the inner surface of the hair cuticle. Some of the pore tubules terminate approximately at right angles on the dendritic membrane, others lie against the membrane. The contact seems to be made via the surface coats of the tubules and the membrane. The structure of pore tubules which had been negatively stained with uranyl acetate is similar to the conventionally thin-sectioned material. The observations provide support for earlier assumptions that pore tubules are the pathways by which odor molecules reach the dendritic membrane. 相似文献
3.
The neurofibrillary tangles that occur in the brain in cases of senile dementia of the Alzheimer type contain a distinctive type of filament, the paired helical filament (PHF). We have developed a method for isolating the tangles postmortem in sufficient yield for structural study of PHFs by electron microscopy of negatively stained and shadowed preparations. This material shows the characteristic helical structure seen in sectioned embedded material. In addition, two striking fragmentation patterns are observed. (a) Some filaments show sharp transverse breaks at apparently random positions along the filament. (b) In a few PHFs one strand is missing for a variable length, whereas the other appears to maintain its structural integrity. The shadowed specimens show the PHF to be wound in a left-handed manner. These observations indicate that the PHF consists of subunits of very limited axial extent arranged along two left-handed helical strands. The visualization of the substructure within the PHFs is rather variable and a model building approach has therefore been adopted, which has allowed the main features seen in the images to be interpreted. The subunit appears to have at least two domains in a radial direction and an axial extent of less than 5 nm. The whole structure can best be described as a twisted ribbon and indeed alkali treatment does untwist PHFs to give flat ribbons. The nature of the proposed model makes it most unlikely that the PHF is formed by a simple collapse of normal cytoskeletal elements, such as neurofilaments. 相似文献
4.
PAUL R. BURTON 《The Journal of eukaryotic microbiology》1970,17(2):295-299
SYNOPSIS. Comparisons are made between sectioned and negatively-stained mitochondria of the ciliate Spirostomum ambiguum. Particulate elements 70–80 A in diameter are associated with the surface of tubular cristae of negatively-stained and disrupted mitochondria; such particles are not seen in sectioned mitochondria fixed in various ways. As measured in sectioned material, the inner mitochondrial membrane forming the tubular cristae is about 100 A thick, while the outer mitochondrial membrane is about 50 A thick and is the more labile of the 2. 相似文献
5.
H. MEYER 《The Journal of eukaryotic microbiology》1968,15(3):614-621
SYNOPSIS. Trypanosoma cruzi in tissue cultures was studied with the electron microscope after double fixation in glutaraldehyde and osmium tetroxide, and embedding in Epon. Previous findings on its fine structure were confirmed, and some new structures were found in the flagellum and kinetoplast-chondriome. In the flagellum, an intraflagellar body was found, similar to that observed in other trypanosomes, beginning at the base of the flagellum and running along the axial fibre bundle thruout its length. The axial fibre bundle is formed by interconnecting tubules, the outer ones apparently smooth, the inner ones with a helical substructure. Lateral extensions from the outer tubules in the flagellar bundle seem to enter the intraflagellar body. The kinetopiast in the leishmania bodies has the same electrondense structure described before. In the trypanosome form it has assumed a large spherical shape, in which the formerly short, compressed fibres have grown in length, are more dispersed and have an irregular shape. They are oriented in the direction of the body's length in parallel array. The whole formation is continuous with a long mitochondrion which begins in the region of the nucleus and extends up almost to the tip of the trypanosome. The matrix of the kinetoplast in these forms is electron-transparent; the matrix of the mitochondria is rather dense. In a few extracellular trypanosomes, a special structure was found in which the kinetoplast is composed of electron-transparent formations, arranged in orderly horizontal lines quite similar to the mitochondrial cristae of the parasite. The significance of this structure is uncertain. 相似文献
6.
Plain and Complex Flagella of Pseudomonas rhodos: Analysis of Fine Structure and Composition 总被引:9,自引:7,他引:2
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Cells of Pseudomonas rhodos 9-6 produce two morphologically distinct flagella termed plain and complex, respectively. Fine structure analyses by electron microscopy and optical diffraction showed that plain flagellar filaments are cylinders of 13-nm diameter composed of globular subunits like normal bacterial flagella. The structure comprises nine large-scale helical rows of subunits intersecting four small-scale helices of pitch angle 25 degrees . Complex filaments have a conspicuous helical sheath, 18-nm wide, of three close-fitting helical bands, each about 4.7-nm wide, separated by axial intervals, 4.7 nm wide, running at an angle of 27 degrees . The internal core has similar but not identical substructure to plain filaments. Unlike plain flagella, the complex species is fragile and does not aggregate in bundles. Mutants bearing only one of two types of flagellum were isolated. Cells with plain flagella showed normal translational motion, and cells with complex flagella showed rapid spinning. Isolated plain flagella consist of a 37,000-dalton subunit separable into two isoproteins. Complex filaments consist of a 55,000-dalton protein; a second 43,000-dalton protein was assigned to complex flagellar hooks. The results indicate that plain and complex flagella are entirely different in structure and composition and that the complex type represents a novel flagellar species. Its possible mode of action is discussed. 相似文献
7.
T. W. Fraser 《Protoplasma》1976,90(1-2):15-31
Summary Potato mop-top virus, one of the most commonly occurring viruses in virus tested stocks of seed potatoes in the United Kingdom induces four different haulm symptoms which are both climate and variety dependent. Ultrastructural examination showed that the aucuba symptom recognizable as bright yellow patches on the leaves, and the mop-top symptom characterised by the dwarfed and bunched habit of the plants, both contained in their leaf cells, tufts and clusters of microtubule-like elements, although the other ultrastructural features associated with each symptom were quite different. These mop-top tubules occurred in the cytoplasm, between the cell wall and the plasma membrane, and in the vacuole, and have been demonstrated in every cell type.The mop-top tubules were 18–22 nm wide with a 2.5–3.0 nm thick wall and often branched. No regular substructure could be discerned. Complete virus particles were rarely seen. These mop-top tubules are compared with plant microtubules and P-protein tubules, and the topic of viral inclusions and their relevance to virus classification is discussed. 相似文献
8.
J. R. McIntosh 《The Journal of cell biology》1974,61(1):166-187
Bridges between microtubules have been studied with the electron microscope in the axostyle of Saccinobaculus and in various tubule systems of chicken testis, including the helix of tubules surrounding the elongating spermatid nucleus and the flagellum of the sperm tail. In addition to the previously described periodic bridges, evidence is presented that nonperiodic bridges exist between certain tubules. An analysis of axial spacing between adjacent nonperiodic bridges suggests that these structures are attached to periodic binding sites on the microtubule wall, but that not all the binding sites are filled. The bridges appear nonperiodic as a result of random occupancy of some fraction of the periodic sites. The distribution of these binding sites is related to the substructure of the microtubule wall as seen with negative staining and optical diffraction. 相似文献
9.
Summary Changes in the spatial relationship between actin filaments and microtubules during the differentiation of tracheary elements (TEs) was investigated by a double staining technique in isolatedZinnia mesophyll cells. Before thickening of the secondary wall began to occur, the actin filaments and microtubules were oriented parallel to the long axis of the cell. Reticulate bundles of microtubules and aggregates of actin filaments emerged beneath the plasma membrane almost simultaneously, immediately before the start of the deposition of the secondary wall. The aggregates of actin filaments were observed exclusively between the microtubule bundles. Subsequently, the aggregates of actin filaments extended preferentially in the direction transverse to the long axis of the cell, and the arrays of bundles of microtubules which were still present between the aggregates of actin filaments became transversely aligned. The deposition of the secondary walls then took place along the transversely aligned bundles of microtubules.Disruption of actin filaments by cytochalasin B produced TEs with longitudinal bands of secondary wall, along which bundles of microtubules were seen, while TEs produced in the absence of cytochalasin B had transverse bands of secondary wall. These results indicate that actin filaments play an important role in the change in the orientation of arrays of microtubules from longitudinal to transverse. Disruption of microtubules by colchicine resulted in dispersal of the regularly arranged aggregates of actin filaments, but did not inhibit the formation of the aggregates itself, suggesting that microtubules are involved in maintaining the arrangement of actin filaments but are not involved in inducing the formation of the regularly arranged aggregates of actin filaments.These findings demonstrate that actin filaments cooperate with microtubules in controlling the site of deposition of the secondary wall in developing TEs.Abbreviations DMSO
dimethylsulfoxide
- EGTA
ethyleneglycolbis(-aminoethyl ether)-N,N,N,N-tetraacetic acid
- FITC
fluorescein isothiocyanate
- MSB
microtubule-stabilizing buffer
- PBS
phosphate buffered saline
- PIPES
piperazine-N,N-bis(2-ethanesulfonic acid)
- TE
tracheary element 相似文献
10.
ELECTRON MICROSCOPE STUDIES OF SPERMATOZOA OF RHYNCHOSCIARA SP : I. Disruption of Microtubules by Various Treatments 总被引:1,自引:1,他引:0
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Jerry W. Shay 《The Journal of cell biology》1972,54(3):598-608
The flagellar complex of the unusual motile spermatozoon of the fungus gnat, Rhynchosciara sp, does not conform to the usual "9 + 2" filament pattern but rather consists of over 350 pairs of filaments (doublet microtubules) distributed in a spiral array. Experiments were designed to disrupt and extract flagellar microtubular components from spermatozoa of the fungus gnat. Pepsin, chymotrypsin, potassium iodide, urea, and heat were used to extract specific portions of microtubule walls Such experiments provide information on the composition of the wall and the existence of wall sites selectively sensitive to various treatments Results obtained include: (a) doublet microtubules are comprised at least in part of protein, and all subunits are probably not identical; (b) a portion of the B subfiber is apparently more sensitive to disruption than other portions of the doublet microtubule; and (c) the ac cessory singlet microtubules may be chemically different from the doublet microtubules 相似文献
11.
Microtubules at the tip of a resting (non-feeding) tentacle are arranged helically in two concentric tube-shaped arrays. The pitches of the helical paths followed by tubules in the two arrays differ. At the start of feeding these microtubules bend along their longitudinal axes and splay outwards and downwards away from the tentacle tip as it ‘everts’. Tubules in the two arrays slideacross each other as this occurs. Comparison of the fine structure of the tips of feeding and resting tentacles with a dynamic model of the microtubular framework indicates that movement of the tubules is not brought about by active sliding of the tubules against each other or by the action of contractile elements attached along the lengths of tubules. The tips of microtubules forming the inner tube may be pulled downwards by contractile elements in the tentacular pellicle; these tubules apparently push those in. the outer tube to their new positions. The pattern of configurational changes in a tentacle tip at the start of feeding appears to be largely defined by the elastic resistance of the microtubules to bending, and the ways in which tubules are packed and linked together and attached to the pellicle. 相似文献
12.
Microtubules and the endoplasmic reticulum are highly interdependent structures 总被引:55,自引:31,他引:24
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The interrelationships of the endoplasmic reticulum (ER), microtubules, and intermediate filaments were studied in the peripheral regions of thin, spread fibroblasts, epithelial, and vascular endothelial cells in culture. We combined a fluorescent dye staining technique to localize the ER with immunofluorescence to localize microtubules or intermediate filaments in the same cell. Microtubules and the ER are sparse in the lamellipodia, but intermediate filaments are usually completely absent. These relationships indicate that microtubules and the ER advance into the lamellipodia before intermediate filaments. We observed that microtubules and tubules of the ER have nearly identical distributions in lamellipodia, where new extensions of both are taking place. We perturbed microtubules by nocodazole, cold temperature, or hypotonic shock, and observed the effects on the ER distribution. On the basis of our observations in untreated cells and our experiments with microtubule perturbation, we conclude that microtubules and the ER are highly interdependent in two ways: (a) polymerization of individual microtubules and extension of individual ER tubules occur together at the level of resolution of the fluorescence microscope, and (b) depolymerization of microtubules does not disrupt the ER network in the short term (15 min), but prolonged absence of microtubules (2 h) leads to a slow retraction of the ER network towards the cell center, indicating that over longer periods of time, the extended state of the entire ER network requires the microtubule system. 相似文献
13.
Paul Kugrens 《Journal of phycology》1983,19(4):507-510
Hexagonal or angular crystalline inclusions in Pleonosporium (Naeg.) Hauck vegetative cells were examined using electron microscopy. Ultrastructural analysis reveals that the inclusions initially contain tubular elements resembling microtubules but, with continued differentiation, are transformed into rod containing crystals. The tubular structures initially measure 25 nm in diameter. Scattered tubules become arranged in a parallel and alternate pattern and undergo subsequent enlargement to approximately 29 nm. Following enlargement, each tubule apparently disaggregates into rods that form a crystal having hexagonally arranged rod-like subunits. It is suggested that these tubules may represent microtubules and the resultant crystals are composed of tubulin. 相似文献
14.
Some structural and cytochemical observations on the axial filament complex of lung-fluke spermatozoa 总被引:3,自引:0,他引:3
P R Burton 《Journal of morphology》1973,140(2):185-195
As seen in transverse section, doublet elements of the axial unit of spermatozoa of Haematolocchus medioplexus, a frog lung-fluke, possess walls made up of protofibrillar subunits 50–60 Å in diameter. The partition between A and B members of a doublet element often show extra protofibrils which may partially occlude the “lumen” of the A tubule. Each A tubule possesses outer and inner lateral arms which repeat at longitudinal intervals of about 215 Å and which appear to be structurally dissimilar; the outer arm is expanded at its free end and the inner arm often connects to the B tubule of the adjacent doublet element. Regularly-spaced radial links connect the central sheath of an inner core complex to the A tubules of the peripheral doublet elements. Tests for magnesium-activated ATPase activity provide evidence that the enzyme is associated with the surfaces of doublet elements and the surface of the central sheath. Finally, study of an axial unit which developed in an abnormal manner suggests that normal differentiation of an axial unit may depend on the elaboration of a core complex and radial links. 相似文献
15.
J. D. Pickett-Heaps 《Planta》1968,81(2):193-200
Summary During oogenesis in Chara fibrosa, and in the enlarging, young daughter coenobia of Volvox spec., microtubule-like structures were found in growing plastids. These were appreciably bigger than the usual 240 Å cytoplasmic microtubules, measuring about 320 Å in diameter; a helical or banded organisation in the wall of these tubules was also evident. The tubules were generally present in greatest numbers when the plastids were elongating or enlarging. 相似文献
16.
Structure of short thick filaments from Limulus muscle 总被引:3,自引:0,他引:3
Shortened Limulus thick filaments, isolated from stimulated muscle, are structurally similar to long filaments, isolated from unstimulated muscle, except for length. Both have 3-fold screw symmetry with a helical repeat at approximately 43 nm, axial spacing of 14.5 nm between successive crowns of crossbridges and 4-fold rotational symmetry as estimated from the Bessel argument, by analysis of optical transforms of electron micrograph negatives of negatively stained samples. Both short and long filaments also have similar radii for the location of their crossbridges, thus similar diameters. Equal numbers of subunits/helical strand are also apparent on images of metal-shadowed long and short filaments. Since these data argue against molecular reorganization during filament shortening, it is suggested that the change in length of Limulus thick filaments may occur by reversible disaggregation of constituent protein molecules. 相似文献
17.
Catherine Henley 《Journal of morphology》1973,140(2):197-213
This is the first demonstration of an apparent acrosome reaction by the use of negative staining (phosphotungstic acid) and electron microscopy. It is also the first report of such a reaction in spermatozoa of an oligochaete annelid (Lumbricus terrestris). The morphology of the negatively stained unreacted acrosomal complexes is entirely comparable to that seen in sectioned material. After reaction, a short straight filament is extruded and the compartmentalization seen in unreacted spermatozoa is no longer visible. In the flagellar axoneme there are short cross-bridges linking the two central singlet microtubules at 150–170 Å intervals. Two fibrous elements are also present, forming, with the singlets, a tetragon. All four of these structures apparently change their elastic properties after incubation in distilled water for two hours, so that instead of following the doublet microtubules in gentle coils, the central complex components fall as a unit in jagged configurations. 相似文献
18.
Subunit structure in the walls of sectioned microtubules was first noted by Ledbetter and Porter (6), who clearly showed that certain microtubules of plant meristematic cells have 13 wall protofilaments when seen in cross section. Earlier, protofilaments of microtubular elements had been described in negatively stained material, although exact counts of their number were difficult to obtain. In microtubular elements of axonemes, some success has been achieved in visualizing protofilaments in conventionally fixed and sectioned material (8, 10); much less success has been achieved in identifying and counting protofilaments of singlet cytoplasmic microtubules. By using glutaraldehyde-tannic acid fixation, as described by Misuhira and Futaesaku (7), Tilney et al. (12) studied microtubules from a number of sources and found that all have 13 protofilaments comprising their walls. These authors note that "...the number of subunits and their arrangement as protofilaments appear universal...". Preliminary studies of ventral nerve cord of crayfish fixed in glutaraldehyde-tannic acid indicated that axonal microtubules in this material possess only 12 protofilaments (4). On the basis of this observation, tannic acid preparations of several other neuronal and non-neuronal systems were examined. Protofilaments in microtubules from these several cell types are clearly demonstrated, and counts have been made which show that some kinds of microtubules have more or fewer protofilaments than the usual 13 and that at least one kind of microtubule has an even rather than an odd number. 相似文献
19.
SYNOPSIS. In an electron microscopic study, counts of peripheral microtubules were made in spheromastigotes and trypomastigotes in tissue cultures of embryonic heart muscle cells. In interphase spheromastigotes there were, at the level of the nucleus, ~ 114 microtubules; in dividing forms, there were ~ 222. In trypomastigotes, the number of microtubules varied according to the level of the section—there were fewer than 40 tubules in the pointed ends of an organism, while in the central segment the number of these elements ranged from 60 to 115. The highest number of microtubules was found in the region containing the Golgi complex. The distance between the microtubules was constant, equalling 44 nm, even at the pointed ends of a trypanosome. This suggests that the microtubules course parallel to one another. Cross sections and randomly arranged, variable length, longitudinal sections of the tubules were noted around the kinetosomes in dividing organisms. 相似文献
20.
Abstract— A method has been developed for the isolation of a previously undescribed fibrous protein from rat brain. The newly isolated material consists of bundles of tightly packed 70-80 Å diameter filaments. Based on studies employing degenerated rat optic nerve, it is proposed that these filaments correspond to the well-described astrocyte filaments observed in sectioned preparations of mammalian brain. The purified filaments are stable over a wide temperature range, are not disrupted by colchicine, and exhibit limited solubility in the absence of denaturants or detergents. In neutral SDS-polyacrylamide gel electrophoresis, the filament protein runs as a single band with an apparent molecular weight of 57,000 daltons. This preparation also migrates as a single band in alkaline urea gels, and as a well-resolved doublet on discontinuous SDS-urea gels. In all three electrophoretic systems, the filament subunits co-migrate with rat brain tubulin. Comparative peptide maps of the filament subunits and tubulin indicate a large degree of homology. Our results suggest that microtubules and astrocyte filaments are composed of the same or very similar protein subunits. 相似文献