The complete amino acid sequence of the low molecular weight cytosolic acid phosphatase

This paper presents the complete amino acid sequence of the low molecular weight acid phosphatase from bovine liver. This isoenzyme of the acid phosphatase family is located in the cytosol, is not inhibited by L-(+)-tartrate and fluoride ions, but is inhibited by sulfhydryl reagents. The enzyme consists of 157 amino acid residues, has an acetylated NH2 terminus, and has arginine as the COOH-terminal residue. All 8 half-cystine residues are in the free thiol form. The molecular weight calculated from the sequence is 17,953. The sequence was determined by characterizing the peptides purified by reverse-phase high performance liquid chromatography from tryptic, thermolytic, peptic, Staphylococcus aureus protease, and chymotryptic digests of the carboxymethylated protein. No sequence homologies were found with the two known acylphosphatase isoenzymes or the metalloproteins porcine uteroferrin and purple acid phosphatase from bovine spleen (both of which have acid phosphatase activity). Two half-cystines at or near the active site were identified through the reaction of the enzyme with [14C] iodoacetate in the presence or in the absence of a competitive inhibitor (i.e. inorganic phosphate). Ac-A E Q V T K S V L F V C L G N I C R S P I A E A V F R K L V T D Q N I S D N W V I D S G A V S D W N V G R S P N P R A V S C L R N H G I N T A H K A R Q V T K E D F V T F D Y I L C M D E S N L R D L N R K S N Q V K N C R A K I E L L G S Y D P Q K Q L I I E D P Y Y G N D A D F E T V Y Q Q C V R C C R A F L E K V R-OH.     

Book Notes

The Systems of Land Tenure in the Kikuyu Land Unit . H. E. L ambert
Hawaiian Antiquities (Moolelo Hawaii ). D avid M alo
Navaho Means People . L eonard M c C ombe , E von Z. V ogt , and C lyde K luckhohn
Paradox and Nirvana . R obert L awson S later
The North American Buffalo. A Critical Study of the Species in Its Wild State . F rank G ilbert R oe
The Wulfing Plates: Products of Prehistoric Americans . V irginia D rew W atson
The Medora Site. West Baton Rouge Parish, Louisiana . G eorge I. Q uimby
The Crable Site, Fulton County, Illinois. A Late Prehistoric Site in the Central Illinois Valley . H ale G illiam S mith
Archaeology of the Columbia-Fraser Region . M arian W. S mith
Cattle Point: A Stratified Site in the Southern Northwest Coast Region . A rden R. K ing
Hyperbrachycephaly as Influenced by Cultural Conditioning . J. F ranklin E wing
Surgery of the Shoulder . A. F. D e P alma
The Mark of Oppression, A Psychological Study of the American Negro . A bram K ardiner and L ionel O vesey
Race Relations: The Interaction of Ethnic Groups . B rewton B erry
The Sociology of Georg Simmel . Translated, edited, and with an introduction by K urt H. W olff
Social Behavior and Personality: Contributions of W. I. Thomas to Theory and Social Research . Edited by E dmund H. V olkart
Handbook of Latin American Studies : 1948, No. 14. F rancisco A guilera , ed
Area Studies in American Universities . W endell C. B ennett     

The complete amino acid sequence of coagulogen isolated from Southeast Asian horseshoe crab, Carcinoscorpius rotundicauda

The complete amino acid sequence of coagulogen purified from the hemocytes of the horseshoe crab Carcinoscorpius rotundicauda was determined by characterization of the NH2-terminal sequence and the peptides generated after digestion of the protein with lysyl endopeptidase, Staphylococcal aureus protease V8 and trypsin. Upon sequencing the peptides by the automated Edman method, the following sequence was obtained: A D T N A P L C L C D E P G I L G R N Q L V T P E V K E K I E K A V E A V A E E S G V S G R G F S L F S H H P V F R E C G K Y E C R T V R P E H T R C Y N F P P F V H F T S E C P V S T R D C E P V F G Y T V A G E F R V I V Q A P R A G F R Q C V W Q H K C R Y G S N N C G F S G R C T Q Q R S V V R L V T Y N L E K D G F L C E S F R T C C G C P C R N Y Carcinoscorpius coagulogen consists of a single polypeptide chain with a total of 175 amino acid residues and a calculated molecular weight of 19,675. The secondary structure calculated by the method of Chou and Fasman reveals the presence of an alpha-helix region in the peptide C segment (residue Nos. 19 to 46), which is released during the proteolytic conversion of coagulogen to coagulin gel. The beta-sheet structure and the 16 half-cystines found in the molecule appear to yield a compact protein stable to acid and heat. The amino acid sequences of coagulogen of four species of limulus have been compared and the interspecies evolutionary differences are discussed.     

Reviews

Books reviewed in this article:
Herbicides and Plant Metabolism (Seminar series 38 Society for Experimental Biology) Ed. by A. D. D odge
Physical Methods in Plant Sciences 3. Vol. 11, Modern Methods of Plant-Analysis – New Series. Ed. by H.-F. L inskens and J. F. J ackson
Analyses of Growth and Development of Xanthium. By R. M aksymowich
Anatomy of European Woods – an atlas for the identification of European trees, shrubs and dwarf shrubs. By F. H. S chweingruber
Chromosomes Today , Volume 10. Ed. by K. F redga , B. A. K ihlman and M. D. B ennett
Pests of Stored Products and their Control. By D. S. H ill
Prospects in Systematics. (Systematics Association Special Volume No. 36.) Ed. by D. L. H awks -W orth
Aquatic Plant Book. By C hristopher D. K. C ook
Biological Invasions of Europe and the Mediterranean Basin. Ed, by F. D e C astri , A. J. H ansen and M. D ebussche
Geography Through Botany. By G. K unkel
Phytoarchaeology. By R. R. B rooks and D. J ohannes     

Nutritional ecology of the formosan subterranean termite (Isoptera: Rhinotermitidae): feeding response to commercial wood species

The feeding preferences of the Formosan subterranean termite, Coptotermes formosanus Shiraki, were tested in three separate experiments on 28 different wood species. Experiment 1 was a multiple-choice test designed to test relative preferences among 24 wood species commercially available in New Orleans, LA. Experiment 2 was a similar study designed to test relative preferences among 21 wood species shown or reported to be unpalatable to the Formosan subterranean termite. Experiment 3 was a no-choice test to examine the feeding deterrence of the 10 least preferred wood species. Preference was determined by consumption rates. Birch (Betula alleghaniensis Britton), red gum (Liquidambar styraciflua L.), Parana pine [Araucaria angustifolia (Bert.) 1, sugar maple (Acer saccharum Marsh.), pecan (Carya illinoensis Wangenh.), and northern red oak (Quercus rubra L.) were the most preferred species by C. formosanus in order of consumption rate. All of these species were significantly more preferred than southern yellow pine (Pinus taeda L.), widely used for monitoring. Sinker cypress [ = old growth bald cypress, Taxodium distichum (L.)], western red cedar (Thuja plicata Donn), Alaskan yellow cedar (Chamaecyparis nootkatensis D. Don), eastern red cedar (Juniperus virginiana L.), sassafras [Sassafras albidum (Nutt.)], Spanish cedar (Cedrella odorata L.), Honduras mahogany (Swietenia macrophyla King), Indian rosewood (Dalbergia latifolia Roxb.), Honduras rosewood (D. stevensonii Standl.), and morado (Machaerium sp.) induced significant feeding deterrence and mortality to C. formosanus. The last eight species produced 100% mortality after 3 mo.     

Book Reviews

Books reviewed in this article:
GENETIC RESOURCES OF PHASEOLUS BEANS. Edited by P. G epts
MITOCHONDRIAL BIOGENESIS. Proceedings of a Royal Society Discussion Meeting held on 28 and 29 May 1987. Edited by C.J. L eaver and D. L onsdale
PROGRESS IN PLANT PROTOPLAST RESEARCH. Edited by K.J. P utte , J.J.M. D ons , H.J. H uizing , A.J. K ool , M. K oornneef and F.A. K rens
FILAMENTOUS FUNGI (LIVING RESOURCES FOR BIOTECHNOLOGY). Edited by D.L. H awksworth and B.E. K irsop     

REVIEWS

The New Genetics in the Soviet Union. By P. S. H udson and R. H. R ichens
Trace Elements in Plants and Animals. By W alter S tiles
Actions of Radiations on Living Cells. By D. E. L ea
Biology of Tissue Cells. By A lbert F ischer
Gall Midges of Economic Importance. By H. F. B arnes , Agricultural and Horticultural Handbooks
DDT The Synthetic Insecticide. By T. F. W est , M.Sc., Ph.D. (Lond.), F.R.I.C. and G. A. C ampbell , M.Sc. (Leeds), F.R.I.C.
Danish Review of Game Biology.     

Besprechungen

Reviewed in this article:
Annual Review of Phytopathology. Herausgegeben von J. G. H orsfall und K. F. B aker .
Paedi, K. and M. V. Tracey, continued by H. F. L inskens and M. V. T racey .
Paech, K. and M. V. Tracey, continued by H. E. L inskens and M. V. T racey . in cooperation with B. D. S anwal , Modern Methods of Plant Analysis.
Sdimalfufi, K., Pflanzenernahrung und Bodenkunde.
Virus diseases of Apples and Pears. Herausgegeben von A. F. P osnette .     

REVIEWS

Plant Anatomy. By K atherine E sau .
Cytochemistry: A Critical Approach. By J. F. D anielli .
Plant Diseases. By F. T. B rooks .
The Fungi. By E rnst A lbert G äumann , translated from the German and arranged as an American text by F rederick L yle W ynd .
Annual Review of Plant Physiology. Vol. iv. Edited by D aniel I. A rnon and L eonard M achlis .
South African Pollen Grains and Spores. Part I. By E. M. van Z inderen B arker .
Plant Growth Substances. By L. J. A udus .
Rice. By D. H. G rist .
The Metabolism of Algae. By E. G. F ogg .     

REVIEWS

Nature's Second Kingdom. By F. D elaporte . Translated by Arthur Goldhammer.
Encyclopedia of Plant Physiology , New Series, Volume 10. Hormonal regulation of development II. The functions of hormones from the level of the cell to the whole plant. Edited by T. K. S cott .
Cell Culture and Somatic Cell Genetics of Plants , Volume 1. Laboratory procedures and their applications. Edited by I. K. V asil .
Databases in Systematics. Edited by R. A llkin and F. A. B isby .
Plant Chemosystematics. By J. B. H arborne and B. L. T urner .
Current Concepts in Plant Taxonomy. Edited by V. H. H eywood and D. M. M oore .
Introduction to Principles of Plant Taxonomy. By V. V. S ivarajan .
Our Green and Living World: The Wisdom to Save It. By E. S. A yensu , V. H. H eywood , G.L. L ucas and R. A. D efillips .
Lipids in Plants and Microbes. By J. L. H arwood and N. J. R ussell .
The Role of Terrestrial Vegetation in the Global Carbon Cycle: Measurement by Remote Sensing. Edited by G. M. W oodwell .
Chromosomes Today , Volume 8. Edited by M. D. B ennett , A. G ropp and U. W olf .     

Book Reviews

Book Reviewed in this article:
Cook, R. J., and K. R Baker , The nature and practice of biological control of plant pathogens.
Grogan, R. G., G. A. Zentmyer and E. B. Cowling (Eds.), Annual Review of Phytopathology.
Zimmermann, M. H. , Xylem Structure and the Ascent of Sap. Springer Series in Wood Science
Dixon, G. R. , Plant Pathogens and their Control in Horticulture.
Harley, J. L., and S. E. Smith , Mycorrhizal Sytnbiosis.
Loewus, F. A., and C. A. Ryan (Eds.) , The Phytochemistry of Cell Recognition and Cell Surface Interactions—Recent Advances in Phytochemistry
Holdsworth, S. D. , The preservation of fruit and vegetable products. Sci. Horticult.     

REVIEWS

Grazing in Terrestrial and Marine Environments (British Ecological Society Symposium No. 4). Edited by D. J. C risp
Fundamentals of Botany Series . Edited by W. A. J ensen and L. G. K avaljian
Multivariate Statistical Analysis for Biologists . By H ilary S eal
Differenciation des Cellules Sexuelles et Eécondation chez les Cryptogames (Protoplasmatologia , Vol. VII/3b). By B. V azart
Allgemeine Botanik . By W. N ultsch
Piante Medicinali, Chimica, Farmacologia e Terapia . Vol. II. By R. B enigni , C. C apra and P. E. C attorini
Thermophilic Fungi . By D onald G. C ooney and R alph E merson
The Genera of Flowering Flants (Angiospermae). Based Principally on the Genera Plantarum of G. Bentham and J. D. Hooker. Dicotyledones . vol. 1. By J. H utchinson
Algal Cultures and Phytoplankton Ecology . By G. E. F ogg
Flora Europaea, Vol . 1. Eycopodiaceae to Plantanaceae . Edited by T. G. T utin , V. H. H eywood , N. A. B urges , D. H. V alentine , S. M. W alters and D. A. W ebb
The Biology of the Algae . By F. E. R ound
Flora of the British Isles Illustrations . Part 4. Monocotyledons . Drawings by S ybil J. R oles
Plants and the Ecosystem . By W. D. B illings . (Fundamentals of Botany Series, edited by W. A. J ensen and L. G. K avaljian
Essays on Crop Evolution . Edited by S ir J oseph H utchinson     

REVIEWS

Book Reviewed in this article:
Pigments in Plants . Ed. by F. C zygan .
Nitrogen and Carbon Metabolism . Ed. by J. D erek B ewley .
Advances in Biochemical Engineering . Vol. 18, Plant Cell Cultures II . Managing Editor: A. F iechter .
The Botany of Auckland . By L ucy M. C ranwell .
Photosynthesis . By D. O. H all and K. K. R ao .
Plants and Mineral Salts . By J. F. S utcliffe and D. A. B aker .
The Archives of the Peat Bogs . By S ir H arry G odwin .
The Biology of Mosses . By D. H. S. R ichardson .     

REVIEWS

Review in this article:
Active Transport and Secretion. Symposium of the Society of Experimental Biology. VIII. Edited by R. B rown add J. F. D anielli .
Protoplasmatologia. Handbuch der Protoplasmaforschung. Hrsg. v. L. V. Heilbrunn u. F. Weber. Band 1/1: Die makromolekulare Chemie und ihre Bedeutung für die Protoplasmaforschung. By H. S taudinger and M. S taudinger .
Flora of Egypt. Vol. III. Angiospermae, part Monocotyledons: Liliaceae—Musaceae. By V ivi T ackholm and M ohammed D rar .
Australian and New Zealand Botany. By J. M c L uckie , M.A., B.Sc. (Glas.), D.Sc. (Syd.) and H. S. M c K ee , B.A., D.Phil. (Oxon).
South African Pollen grains and Spores, Part I. By E. M. van Z inderen B akker .     

Reviews

Comparative Animal Physiology. By C. L add P rosser et al.
Organisme et Sexualité. By M. C aullery .
Embryos and Ancestors. By G. R. de B eer .
The Association of Ants with Aphids and Coccids. By G. E. J. N ixon .
Soil and Fresh-Water Nematodes. By T. G oodey
Die Überträger pflanzlicher Viruskrankheiten (The vectors of plant viruses). By K urt H einze .
Leaf Curl Disease of Cotton. By S. A. J. T arr .
Introduction to Mycology. By J. A. M ac D onald .
Pesticide Handbook 1951. Edited by D. E. H. F rear .
Pflanzliche Infektionslehre. By E rnst G äumann .
A Dictionary of Biology. By M. A bercrombie , C. J. H ickman and M. L. J ohnson .
Soviet Genetics. By A. G. M orton .
Gall Midges of Economic Importance. Vol. v. Gall Midges of Trees. By H. F. B arnes .
Four Thousand Million Mouths. Scientific Humanism and the Shadow of World Hunger. Edited by F. L e G ros C lark and N. W. P irie .     

REVIEWS

Book reviewed in this article:
A Dictionary of the Flowering Plants and Ferns . By the late J. C. W illis . Seventh edition, revised by H. K. A iry S haw .
The Principles of Pollination Ecology . By K. F aegri and L. van D er P ijl .
Studies in Biology, No. 5, Plant Taxonomy . By V. H. H eywood .
Deadly Harvest . By J ohn M. K ingsbury .
Plant Physiology . By H enrik G. L undegardh . Translated by F. M. I rvine , Translation edited by W. O. J ames .
The Biology of Lichens . By M ason E. H ale J r . In: Contemporary Biology Series, General editors E. J. W. B arrington and A. J. W illis .
Plant Biology Today . Second edition. Ed. by W. A. J ensen and L. G. K avaljian .
Organisation in Plants . Second edition. By B. M. B aron .
Fortschritte der Botanik , Band 28. Ed. by E. B ünning , H. E llenberg , K. E sser , H. M erxmuller and P. S itte .
Taxonomy of Flowering Plants . Second edition. By C. L. P orter .
The Study of Biology . By J. J. W. B aker and G. E. A llen .
Experiments in the Study of Biology . By J. J. W. B aker , G. E. A llen , E. G age and S. K. W ebster .
Grundriss der Phytopathologie iind des Pflanzenschutzes . By H ans -A lfred K irchner .
Bibliography of Seeds . Compiled and edited by L. V. B arton .
Pollen Grains of Western Himalaxan Plants . By P. K. K. N air .
Families of Flowering Plants in Ethiopia . By W. C. B urger .     

Reviews

Books reviewed in this article:
Root Development and Function. Ed. by P. J. G regory , J. V. L ake and D. A. R ose
Modern Methods in Plant Analysis. Volume 3. Gas Chromatography/Mass Spectrometry. Edited by H. F. L inskens and J. F. J ackson
Genetic Differentiation and Dispersal in Plants. Edited by P. J acquard , G. H eim and J. A ntonovics
Tropical Forest and its Environment . By K. A. L ongman and J. J enik
Ecology of Biological Invasions. Edited by R. H. G roves and J. J. B urdon
Ecology of Microbial Communities: 41st Symposium of The Society for General Microbiology. Ed. by M. F letcher , T. R. G. G ray and J. G. J ones     

REVIEWS

Book reviewed in this article:
Respiratory Metabolism in Plants . By H arry B eevers .
Plant Pathology: an Advanced Treatise . Volume 2: The Pathogen. Edited by J. G. H orsfall and A. E. D imond .
Flammenphotometrie . By R. H errmann and C. T. J. A lkemade .
Soil Conditions and Plant Growth . By S ir E. J ohn R ussell .
Bacterial Plant Pathogens . By C. S tapp . Translated by A. S choenfeld .
Die Pflanzliche Zellwand . By A. F rey -W yssling
Maps of Distribution of Norwegian Vascular Plants . Edited by K nut F aegri , O lav G jaerevoll , J ohannes L id , R olf N ordhagen . Volume I. Coast Plants by K nut F aegri .
Les forêts acidophiles du Jura. Materiaux pour le levé géobotanique de la Suisse , Fascicule 38. By J. L. R ichard .
A Symposium on Light and Life . Edited by W illiam D. M celroy and B entley G lass .
Woody Plants of Ghana . By F. R. I rvine .     

REVIEWS

Books reviewed in this article:
Thonner's Analytical Key to the Families of Flowering Plants. By R. G eesink , A. J. M. L eeuwenberg , C. E. R idsdale and J. F. V eldkamp
The Plant Cuticle. Ed. by D. F. C utler , K. L. A lvin and C. E. P rice
Introduction to Ecological Biochemistry . By J. B. H arborne
Vegetation Mitteleuropas mit den Alpen. By H einz E llenberg
Chloroplasts and Mitochondria. By M. T ribe and P. W hittaker
The Physiology and Biochemistry of Drought Resistance in Plants. Ed. by L. G. P aleg and D. A spinall     

BUCHBESPRECHUNGEN

S tephan , H.; B aron , G.; F rahm , H. D.: Comparative Brain Research in Mammals.
S uzuki , D. T.; G riffiths , A. J. F.; M iller , J. H.; L ewontin , R. C.: G enetik. Übersetzt von S. A chten und P. B öhm .
K ämpfe , L othar (ed.): Evolution und Stammesgeschichte der Organismen. Bearbeitet von 5 Fachwissenschaftlem. 3., neubearbeitete und erweiterte Auflage.