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1.
Gassler N Bohn J Schnölzer M Scheuerer J Obermüller N Otto HF Autschbach F 《Histology and histopathology》2003,18(1):121-127
Calnexin (Cnx) has been characterized as a membrane-bound protein that transiently interacts in a unique chaperone system with newly synthesized glycoproteins in order to allow the establishment of their proper tertiary and, in most cases, quarternary structures. The aim of the study was to identify and to locate the expression of Cnx in the three major salivary glands of humans by different methods. Strong expression of Cnx protein and mRNA were generally found in serous salivary secretory units. With regard to mucous secretory units, expression of Cnx was only detectable at a low level in mucous acinar cells of sublingual glands, but not of submandibular glands. Expression of Cnx was always preserved in the surface epithelium of intralobar and interlobular duct segments. In addition, expression of Cnx was detected in sebaceous glands of parotid tissues, with a distribution pattern resembling that seen in sebaceous glands of the normal skin. In conclusion, production of saliva is associated with the expression of Cnx. Synthesis of molecules in mucous secretory units is not necessarily associated with a strong Cnx expression, whereas synthesis in serous secretory units apparently is. The tissue-specific Cnx expression is also paralleled by the observation that the secretions produced by the major salivary glands differ in their composition and amount. 相似文献
2.
Myoepithelial cells were present between the basal lamina and the acinar secretory cells of human labial salivary glands. In form and disposition, they resembled myoepithelial cells in the major salivary glands. Many of these cells possessed single cilia on their upper surfaces. Such cilia occasionally extended into invaginations of the overlying secretory cell. The intercalated ducts were variable in occurrence. Their epithelium ranged from columnar to squamous, and showed few signs of secretory activity. Few intralobular ducts possessed basal striations. While mitochondria were abundant in non-striated cells, they were randomly disposed in both basal and apical cytoplasm, and the basal plasmalemma showed only occasional infoldings. The paucity of true striated ducts in labial salivary glands may be responsible for the high concentration of sodium and chloride in unstimulated labial gland salivary secretions. 相似文献
3.
Oviducal glands throughout the gonad development stages: a case study of Octopus mimus (Cephalopoda)
Alberto Olivares Victor Leyton Oscar Zuñiga Carlos Rosas Irma Northland-Leppe 《Molluscan research.》2017,37(4):229-241
The oviducal glands (ODG) play a crucial role in octopus reproduction. Herein, structural changes of each section of the ODG of Octopus mimus are described histologically throughout the gonad development stages (GDS). To do this, the epithelial height, stereociliated or non-stereociliated epithelium, nucleus type (pycnotic or non-pycnotic), epithelial secretions and the value range of the macroscopic maturity index (MaMI), which directly involves ODG status, were measured. The ODG are internally constituted of two glandular units (central and peripheral glands) and one set of receptacles (the spermathecae). High epithelia (40 to 80?μm) were observed in both gland units during periods with low MaMI values (< 0.1) corresponding to III-mature and IV-pre-spawning. The stereociliated epithelium was only apparent in II-maturing and III-mature in both gland units. The nuclei were noticeably pycnotic in the central gland during III-mature, IV-pre-spawning and V-spawning, but pycnotic in the peripheral gland only during VI-depletion. The epithelium was disorganised during VI-depletion, while sulphated acid mucin was only present during III-mature in the central gland. The epithelium transformations during the GDS are related to the functions of the gland units and to their multiple secretions. The ODG histology complements the GDS and provides better reproductive status assessment. 相似文献
4.
Odhiambo TR 《Tissue & cell》1969,1(1):155-182
The accessory reproductive glands of the male desert locust were studied by histological, histochemical, and phase-contrast techniques. It was found that the characteristics of the glandular epithelium and their corresponding secretions permit the division of the accessory glands into nine distinct types. Three types produce coarsely granular mucopolysaccharide secretions (glands 1, 11, and 12); three types produce finely fibrous mucopolysaccharide secretions (glands 2, 4, and 7-10, 13-15); one type produces a globular mucopolysaccharide or mucoproteinaceous secretion (gland 6); one type produces an acidic lipoprotein complex (glands 3 and 5); and one is the functional seminal vesicle (gland 16). Consequently, the various secretions are separated as a result of a vertical segregatign of the various cell types that are responsible for glandular activity. 相似文献
5.
The morphology, blood and nerve supply of the parotid salivary glands of the one-humped camel were studied in detail. The intraglandular portion of the duct system was also examined. The histological and histochemical studies showed that the parotid salivary glands of the camel are of the tubuloacinar type and are serumocoid in nature. The secretory acini and tubules show themselves in 3 different forms according to the different phases of their secretory cycle. The duct system of the gland contains goblet cells between its lining epithelium. The intercalated ducts show ampullation followed by narrowing that help in mixing the secretion. Intraepithelial glands are found in the terminal part of the parotid duct. 相似文献
6.
The ability of coxsackievirus B-1 to pass the barriers of the circulatory system into whole saliva has been shown previously. In this investigation, the major salivary glands and the oral mucosa were studied, and their role as participants in the excretion of coxsackievirus B-1 during viremia was evaluated. The effect of the salivary-gland stimulant pilocarpine nitrate on both the salivary flow rate and the recovery of virus during viremia was determined. A comparison was made between the amount of virus recovered from whole saliva during viremia in animals deficient in one or both of the major salivary-gland pairs and animals with a complete complement of salivary glands. The salivary glands in other animals were cannulated, and pure glandular secretions were collected during viremia and assayed for the presence of virus The amount of virus passing from the capillaries of the oral mucosa to the surface was also determined to evaluate this route as a possible site for the excretion of virus into saliva during viremia. The major salivary glands did not excrete appreciable quantities of virus during viremia. The submaxillary-gland secretions did not contain virus, and the parotid-gland secretions showed virus only at extremely high blood virus levels. Either removal of the major salivary glands or decreased salivary flow rates increased the concentration of virus in whole saliva. This observation suggested that the production of saliva by the major salivary glands tends to dilute the virus in the oral cavity. A 0.88-cm2 sample of the oral mucosa excreted significantly large amounts of virus during viremia and suggested that the passage of virus through the oral mucosa was the major route for the excretion of virus into saliva during viremia. 相似文献
7.
Spatial organization of a chromosome in a nucleus is very important in biology but many aspects of it are still generally unresolved. We focused on tissue-specific features of chromosome architecture in closely related malaria mosquitoes, which have essential inter-specific differences in polytene chromosome attachments in nurse cells. We showed that the region responsible for X-chromosome attachment interacts with nuclear lamina stronger in nurse cells, then in salivary glands cells in Anopheles messeae Fall. The inter-tissue differences were demonstrated more convincingly in an experiment of two distinct chromosomes interposition in the nucleus space of cells from four tissues. Microdissected DNA-probes from nurse cells X-chromosome (2BC) and 3R chromosomes (32D) attachment regions were hybridized with intact nuclei of nurse cells, salivary gland cells, follicle epithelium cells and imaginal disсs cells in 3D-FISH experiments. We showed that only salivary gland cells and follicle epithelium cells have no statistical differences in the interposition of 2BC and 32D. Generally, the X-chromosome and 3R chromosome are located closer to each other in cells of the somatic system in comparison with nurse cells on average. The imaginal disсs cell nuclei have an intermediate arrangement of chromosome interposition, similar to other somatic cells and nurse cells. In spite of species-specific chromosome attachments there are no differences in interposition of nurse cells chromosomes in An. messeae and An. atroparvus Thiel. Nurse cells have an unusual chromosome arrangement without a chromocenter, which could be due to the special mission of generative system cells in ontogenesis and evolution. 相似文献
8.
The acinar salivary glands of the cockroach, Periplaneta americana, are innervated by dopaminergic and serotonergic nerve fibers. Serotonin stimulates the secretion of protein-rich saliva, whereas dopamine causes the production of protein-free saliva. This suggests that dopamine acts selectively on ion-transporting peripheral cells within the acini and the duct cells, and that serotonin acts on the protein-producing central cells of the acini. We have investigated the pharmacology of the dopamine-induced secretory activity of the salivary gland of Periplaneta americana by testing several dopamine receptor agonists and antagonists. The effects of dopamine can be mimicked by the non-selective dopamine receptor agonist 6,7-ADTN and, less effectively, by the vertebrate D1 receptor-selective agonist chloro-APB. The vertebrate D1 receptor-selective agonist SKF 38393 and vertebrate D2 receptor-selective agonist R(-)-TNPA were ineffective. R(+)-Lisuride induces a secretory response with a slower onset and a lower maximal response compared with dopamine-induced secretion. However, lisuride-stimulated glands continue secreting saliva, even after lisuride-washout. Dopamine-induced secretions can be blocked by the vertebrate dopamine receptor antagonists cis(Z)-flupenthixol, chlorpromazine, and S(+)-butaclamol. Our pharmacological data do not unequivocally indicate whether the dopamine receptors on the Periplaneta salivary glands belong to the D1 or D2 subfamily of dopamine receptors, but we can confirm that the pharmacology of invertebrate dopamine receptors is remarkably different from that of their vertebrate counterparts. 相似文献
9.
Fine structure of the excretory system of the deep posterior (Ebner's) salivary glands of the human tongue 总被引:1,自引:0,他引:1
Human deep posterior lingual glands (von Ebner's glands) are located beneath the circumvallate papillae. They are formed by tubuloalveolar adenomeres, intercalated ducts and excretory ducts coming together in the main excretory duct. The tubuloalveolar cells, pyramid-shaped, show large and dense secretory granules (clear cored) throughout the cytoplasm, rare basal folds and packed cisternae of rough endoplasmic reticulum (RER) at the basal pole. The columnar cells of the intercalated ducts are arranged in a monolayer. They are characterized by dense, clear-core secretory granules (mostly in the apical cytoplasm), a basal nucleus, well-developed RER and Golgi apparatus, and thin filaments distributed in supra- and perinuclear cytoplasm. Striated ducts are absent. Excretory ducts, coming together in the main duct, are lined by a bistratified epithelium. The inner layer consists of columnar cells showing bundles of tonofilaments with scarce secretory activity. The outer layer is composed of basal cells lying on the basal lamina. The main excretory duct, which opens at the bottom of the vallum, shows a stratified epithelium. The outer side is composed of 2-3 layers of malpighian cells lying on the basal lamina. The inner side consists of a single layer of cuboidal-columnar cells with dense apical granules and well-developed organelles synthesizing and condensing secretions. These cells interpolate with goblet cells, rare mitochondria-rich cells, ciliated cells and numerous small globous cells showing a clear matrix and lacking secretory granules. The cilia show a 9 + 2 microtubular structure with basal bodies provided with striated rootlets. Myoepithelial cells surround with their processes the basal portions of the secretory cells and the intercalated ducts. The conclusions concern some comparative aspects and some hypothesis on the functional role of goblet cells, ciliated cells and epithelial cells lining the different ducts, also in relation to the final secretory product. 相似文献
10.
T Dalton 《Comp. Biochem. Physiol. C, Comp. Pharmacol. Toxicol.》1987,88(2):233-239
1. Continual stimulation of frog skin epithelium and the salivary glands of the insects Calliphora and Musca with 5-hydroxytryptamine (5-HT) leads to desensitisation, i.e. the tissue fails to respond to the application of further 5-HT. 2. Incubation of desensitised frog skin and Musca salivary glands with either N-acetyl neuraminic acid or inositol partially restored the 5-HT responses whilst incubation with a combination of N-acetyl neuraminic acid and inositol gave additive effects on the recovery of the 5-HT responses. 3. Incubation of desensitised salivary glands of Calliphora with inositol totally restored the 5-HT response whilst incubation with N-acetyl neuraminic acid had no effect. 4. It is concluded that desensitisation involves depletion of secondary messenger from the tissues coupled with receptor degradation and that considerable differences exist in the turnover of 5-HT receptors, the receptors in Musca salivary glands being highly labile, those in Calliphora salivary glands highly stable and those of frog skin epithelium being intermediate in their stability. 相似文献
11.
Pekka Nuorteva 《Entomologia Experimentalis et Applicata》1958,1(1):41-49
Zusammenfassung Im Speichel der Rhynchoten gibt es verschiedene Verdauungsenzyme, die eine weitgehende Anpassung hinsichtlich der Art der Nahrung aufweisen und bei der Verdauung wichtig sind. Zugleich gibt es im Rhynchotenspeichel auch Pflanzenwuchs hemmende Stoffe Auxine und Viren, die alle Krankheitszustände bei den Nahrungspflanzen verursachen welche ihrerseits die Lebensbedingungen der Schädlinge fördern.
Summary The above article is a short survey of the relation between the salivary secretions of the Hemiptera and their host plants.In analyses of the composition secretions in the salivary glands of phytophagous Homoptera and Heteroptera, the most extensive work has been done on the digestive enzymes. Proteases, amylases, saccharase, maltase, pectinase and lipase have been detected, but most commonly only 2–3 of these occurs in any one species. An adaptation of the enzyme complement to the diet is evident and in this respect the feeding site used by the insect is especially decisive to the enzyme composition of the saliva. Proteases and amylases occur in the saliva of mesophyll feeders, but are absent in phloem feeders, for which they are useless because, from the insect's point of view, the food is already digested. The adaptation of the salivary enzymes to the nature of the food seems to be largely inherited for a diet containing nothing but sucrose does not induce adaptive changes in the enzyme content of the salivary glands. By contrast, papain seems to be transferred from a synthetic food to the salivary glands.The disease symptoms caused in plants by the salivary toxins of Homoptera and Heteroptera have many similarities to those caused by abnormal amounts of growth hormones. Indole-tri-acetic acid has been detected in extracts of crushed aphids and leafhoppers, although no growth stimulating hormones have been detected in salivary glands dissected from several heteropterous bugs and one aphid. On the contrary, substances inhibiting plant growth exist in the salivary glands of many Heteroptera and in at least one aphid. In some experiments with a heteropterous bug, indole-triacetic was observed to have been transferred from a synthetic diet to the salivary glands. It seems possible that auxins, enzymes and other phytotoxic substances occurring in the salivary glands of insects may at last in some cases originate from the host plant and are not produced by the insect.The salivary enzymes without doubt play an important role in the digestion of the insects secreting them. They are important also in the differentation of Homoptera and Heteroptera to different modes of life. The auxins or auxin inhibitors, as wel as all phytotoxic substances in the salivary glands of Homoptera and Heteroptera are significant for these animals by changing the physiological state of the host plant in such a direction that its suitability as a source of food increases.相似文献
12.
Cephalaspideans are a group of opisthobranch gastropods that comprises carnivorous and herbivorous species, allowing an investigation of the relationship between these diets and the morphofunctional features of the salivary glands. In this study, the salivary glands of the carnivorous cephalaspidean Philinopsis depicta were observed by light and electron microscopy. The secretory epithelium of these ribbon-shaped glands is formed by ciliated cells, granular cells and cells with apical vacuole. In ciliated cells the nucleus and most cytoplasmic organelles are located in the wider apical region and a very thin stalk reaches the base of the epithelium. These cells possess significant amounts of glycogen. Granular cells are packed with electron-dense secretory granules and also contain several cisternae of rough endoplasmic reticulum and Golgi stacks. The other type of secretory cell is mainly characterized by the presence of a large apical vacuole containing secretion. These cells possess high amounts of rough endoplasmic reticulum cisternae and several Golgi stacks. Vesicles with peripheral electron-dense material are also abundant, and seem to fuse to form the apical vacuole. The available data point out to a significant difference between the salivary glands of carnivorous and herbivorous cephalaspidean opisthobranchs, with an intensification of protein secretion in carnivorous species. 相似文献
13.
《International Journal of Insect Morphology and Embryology》1990,19(3-4):195-209
Cytological variations of the median and the 2 lateral accessory glands of Bruchidius atrolineatus Pic (Coleoptera : Bruchidae) were examined as a function of age and the reproduction of the male. In sexually active virgin males, the secretory epithelium is columnar at emergence, but progressively flattens, and the secretions formed and stored by its cells are expelled by exocytosis into the glandular lumen. After 10 days, the male accessory glands exhibit a stage of repletion, characteristic of glands temporarily storing their secretions in their lumen. In diapausing males, the genital tract is relatively undeveloped and the accessory glands are reduced to tubules, whose lumen, surrounded by an epithelium composed of narrow cells, contains little secreted material. The presence of secretion aggregates in the secretory epithelial cells, the abundance of rough endoplasmic reticulum in them, and the release of a part of their secretions into the glandular lumen, indicate that reproductive diapause in B. atrolineatus is characterized by a decrease in the reproductive function. and not its total arrest. 相似文献
14.
Paul David Shirk Paul Alfred Roberts Chee Hark Harn 《Development genes and evolution》1988,197(2):66-74
Summary The late larvae of Drosophila gibberosa Patterson and Mainland choose different pupariation sites than the larvae of Drosophila melanogaster Meigen. Since the larvae of D. gibberosa do not attach themselves to the substratum, the salivary glands contain only a small amount of the glue proteins before pupariation. Proteins comprising the salivary gland secretions of late larvae of these two species were compared and found to be qualitatively quite different. Only five polypeptides with the same molecular masses were identified in both species. The rate of protein synthesis in the salivary glands of D. gibberosa continued to increase through the late larval stage and pupariation. As a consequence, the total amount of protein contained in the salivary glands also continued to increase after pupariation. To demonstrate temporal changes in protein synthesis from 48 h before pupariation to 28 h after pupariation, newly synthesized polypeptides were pulse labeled by culturing salivary glands in vitro. The patterns of polypeptide synthesis fell into four major groups depending upon whether the synthesis of a protein stopped shortly after pupariation, stopped during late pupariation, increased at pupariation, or was initiated after pupariation. Changing patterns of protein synthesis are correlated with the known changes in gene puffing during this developmental period. 相似文献
15.
The development of lingual glands in the domestic duck (Anas platyrhynchos f. domestica): 3D‐reconstruction,LM, and SEM study
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The major salivary glands of birds develop by branching or elongation of the epithelial cords. The development of the minor salivary glands in form of the lingual glands has never been described. Among birds, only Anatidae have three types of the lingual glands: rostral, caudo‐lateral, and caudo‐medial lingual glands. The study aims to characterize the manner and rate of the lingual glands development in the domestic duck and their topographical arrangement relative to the hyoid apparatus. The study reveals that all three types of the lingual glands develop by branching. We describe five stages of the lingual glands development in the domestic ducks: prebud, initial bud, pseudoglandular, canalicular, and terminal bud stage. The pattern of the lingual glands development in birds is similar to that described for mammals, with the exception, that the terminal buds are formed at the same time as the lumen of the glands. Generally, the rostral lingual gland starts to branch earlier than the caudal lingual glands. The 3D‐reconstruction shows the location and direction of lingual gland development relative to the entoglossal cartilage and basibranchial bone. Light microscopy and scanning electron microscopy allow to characterize the histogenesis of the embryonic epithelium into glandular epithelium. At a time of hatching only secretory units of caudal lingual glands resemble the secretory units of the adult domestic duck. The rostral and caudo‐lateral lingual glands are arranged on the sides of the entoglossal cartilage and basibranchial bone and caudo‐madial lingual glands are located over the basibranchial bone. We suggest that such an arrangement of the lingual glands in the domestic duck is important during food intake and responsible for reduction of friction and formation of food bites. 相似文献
16.
A study has been made on the effect of dopamine on salivary gland secretion rates from isolated locust salivary glands. Application of dopamine induced a concentration-dependent secretion with an IC(50) of approximately 0.3 microM. We investigated the pharmacological profile of this receptor using dopaminergic agonists and antagonists. The effects of dopamine could be mimicked by the selective D1 agonist SKF82958, but not by the D2 agonist TNPA-HCl. The receptor also showed selectively towards certain D1 agonists. SKF82958 was more potent at inducing secretion than SKF81297. We found that dopamine-induced salivary secretions were blocked by the selective D1 antagonist SCH23390, whereas the D2 antagonist sulpiride was relatively ineffective. The cAMP analogue 8-Bromo cAMP also increased secretion rates from isolated salivary glands. These data and the rank order of potency of the agonists and antagonists in this screen suggest that this receptor is a D1-type receptor. 相似文献
17.
The amount of histone H1 relative to core histones has been determined in three Drosophila species (D. melanogaster, D. texana and D. virilis) in chromatin from several tissues differing in chromatin structure and genetic activity. Low levels of H1 were found in relatively undifferentiated, early embryos as well as in a line of cultured cells. In late embryos the content of H1 was highest in D. virilis which possesses larger amounts of and a partially more compacted constitutive heterochromatin than the two other species. Polytene chromatin from larval salivary glands showed increased levels of H1 compared with diploid chromatin and the degree of phosphorylation of this histone was relatively low. The degree of phosphorylation of H2A was found to be drastically reduced in polytene as compared with diploid embryonic chromatin, which parallels the extensive underreplication of constitutive heterochromatin. Also, in diploid chromatin a qualitative correlation was observed between the relative amounts of heterochromatin and the levels of H2A phosphorylation. These findings suggest a connection between H2A phosphorylation and heavy compaction of interphase chromatin. 相似文献
18.
Shen L Suresh L Wu J Xuan J Li H Zhang C Pankewycz O Ambrus JL 《Journal of immunology (Baltimore, Md. : 1950)》2010,185(10):6355-6363
The etiology of salivary gland injury in primary Sj?gren's disease is not well understood. We have previously described a mouse model of Sj?gren's disease, IL-14α transgenic (IL14αTG) mice, which reproduces many of the features of the human disease. We now demonstrate a critical role for lymphotoxin α (LTA) in the pathogenesis of Sj?gren's disease in IL14αTG mice. IL14αTG mice express LTA mRNA in their salivary glands and spleen and produce soluble LTA protein in their salivary secretions. When IL14αTG mice were crossed with LTA(-/-) mice, the IL14αTG.LTA(-/-) mice retained normal salivary gland secretions and did not develop either lymphocytic infiltration of their salivary glands or secondary lymphomas. However, both IL14αTG and IL14αTG.LTA(-/-) mice produced similar amounts of IFN-α and had similar deposition of autoantibodies in their salivary glands. Both IL14α and IL14α/LTA(-/-) mice had similar B cell responses to T-dependent and T-independent Ags, L-selectin expression, and expression of RelA, RelB, and NF-κB2 in their spleens. These studies suggest that LTA plays a critical role in the local rather than systemic inflammatory process of Sj?gren's disease. Furthermore, local production of soluble LTA in the salivary glands of IL14αTG mice is necessary for the development of overt Sj?gren's disease. Autoantibody deposition alone is not sufficient to produce salivary gland dysfunction. We also demonstrate that LTA is increased in the salivary gland secretions and sera of patients with Sj?gren's disease, further strengthening the biological relevance of the IL14αTG model to understanding the pathogenesis of human disease. 相似文献
19.
Dried saliva spot sampling is a minimally invasive technique for the spatial mapping of salivary protein distribution in the oral cavity. In conjunction with untargeted nano‐flow liquid chromatography tandem mass spectrometry (nanoLC–MS/MS) analysis, DSS is used to compare the proteomes secreted by unstimulated parotid and submandibular/sublingual salivary glands. Two hundred and twenty proteins show a statistically significant association with parotid gland secretion, while 30 proteins are at least tenfold more abundant in the submandibular/sublingual glands. Protein identifications and label‐free quantifications are highly reproducible across the paired glands on three consecutive days, enabling to establish the core proteome of glandular secretions categorized into eight salivary protein groups according to their biological functions. The data suggest that the relative contributions of the salivary glands fine‐tune the biological activity of human saliva via medium‐abundant proteins. A number of biomarker candidates for Sjögren's syndrome are observed among the gland‐specifically expressed proteins, which indicates that glandular origin is an important factor to consider in salivary biomarker discovery. 相似文献