Identification of pathogenic dematiaceous fungi and related taxa based on large subunit ribosomal DNA D1/D2 domain sequence analysis

The nucleotide sequences of the D1/D2 domains of large subunit (26S) ribosomal DNA for 76 strains of 46 species of pathogenic dematiaceous fungi and related taxa were determined. Intra-species sequence diversity of medically important dematiaceous fungi including Phialophora verrucosa, Fonsecaea pedrosoi, Fonsecaea compacta, Cladophialophora carrionii, Cladophialophora bantiana, Exophiala dermatitidis, Exophiala jeanselmei, Exophiala spinifera, Exophiala moniliae, and Hortaea werneckii were extremely small; as few as 0 changes were detected in C. bantiana, Fonsecaea and Exophiala species, 1 bp in C. carrionii and H. werneckii, and 2 bp in P. verrucosa. Inter-species nucleotide diversity between most species was higher. These data suggested that the D1/D2 domain is sufficiently variable for identification of pathogenic dematiaceous fungi and relevant species. The phylogenetic trees constructed from the sequence data revealed that most human pathogenic species formed a single cluster and that Cladosporium and Phialophora species were distributed polyphyletically into several clusters.     

Taxonomy of Exophiala werneckii and its relationship to Microsporum mansonii

Study of the neotype culture of Exophiala werneckii (Cladosporium werneckii), as well as additional isolates, has shown that the yeast-like and hyphal conidiogenous cells are annellides. E. werneckii cannot be accommodated in Cladosporium, a genus characterized by catenulate holoblastic coanidia. E. werneckii and the dematiaceous hyphomycete that Castellani misidentified as Microsporum mansonii (Cladosporium mansonii) are two entirely different fungi. M. mansonii is considered to be a synonym of the Malassezia furfur since both names were used to describe in tissue the etiologic agent of pityriasis versicolor.     

Upper Devonian Sponges from the Holy Cross Mountains, Central Poland

The rich fauna of Late Devonian (Late Frasnian) siliceous sponges from the Holy Cross Mountains, Poland is composed of 15 species and 11 genera. Both astylospongid demosponges (lithistids) and hexactinosan hexactinellids are present. The following new genera and/or species are proposed: D regulara Rigby and Pisera sp. nov., Jazwicella media Rigby and Pisera gen. et sp. nov., Astyloscyphia irregularia Rigby and Pisera gen. et sp. nov., A. turbinata Rigby and Pisera gen. et sp. nov., Astylotuba modica Rigby and Pisera gen. et sp. nov., Paleoregulara cupula Rigby and Pisera gen. et sp. nov., Paleoramospongia bifurcata Rigby and Pisera gen. et sp. nov., Cordiospongia conica Rigby and Pisera gen. et sp. nov., Paleocraticularia elongata Rigby and Pisera gen. et sp. nov., P gigantia Rigby and Pisera gen. et sp. nov., Polonospongiadevonica Rigby and Pisera gen. et sp. nov., P fistulata Rigby and Pisera gen. et sp. nov., Urnospongia modica Rigby and Pisera gen. et sp. nov., and Conicospongia annulata Rigby and Pisera gen. et sp. nov. The investigated fauna contains the youngest astylospongiids known and the oldest well-preserved, and most diversified Palaeozoic hexactinosans. The sponge fauna constituted a significant element of a brachiopod-coral-sponge assemblage that inhabited a deep slope of the local Dyminy Reef structure, during its final phase of growth, in a clearly hemipelagic setting. This fauna is limited to the intrashelf depression within an incipiently drowned carbonate platform.     

Unification of the genera Nonlabens, Persicivirga, Sandarakinotalea and Stenothermobacter into a single emended genus, Nonlabens, and description of Nonlabens agnitus sp. nov

Phylogenetic analyses based on 16S rRNA gene sequences showed that a bacterial isolate, designated JC2678(T), represents a distinct phyletic line within the suprageneric monophyletic clade containing the genera Nonlabens, Persicivirga, Stenothermobacter and Sandarakinotalea. The polyphasic data presented in this study demonstrated that the members belonging to the Nonlabens-like clade overall constitute a single genus. Therefore, it is proposed to transfer the members of genera Persicivirga O'Sullivan et al. 2006, Stenothermobacter Lau et al. 2006 and Sandarakinotalea Khan et al. 2006 to the genus Nonlabens Lau et al. 2005. Thus, P. dokdonensis (Yoon et al. 2006) Nedashkovskaya et al. 2009, P. ulvanivorans Barbeyron et al. 2010, P. xylanidelens O'Sullivan et al. 2006, Sandarakinotalea sediminis Khan et al. 2006 and Stenothermobacter spongiae Lau et al. 2006 should be transferred to Nonlabens dokdonensis comb. nov., Nonlabens ulvanivorans comb. nov., Nonlabens xylanidelens comb. nov., Nonlabens sediminis comb. nov. and Nonlabens spongiae comb. nov., respectively. In addition, strain JC2678(T) (=KACC 14155(T)=JCM 17109(T)) is proposed to constitute a novel species belonging to the genus Nonlabens with the name of Nonlabens agnitus sp. nov.     

中国及周边国家的伏绕眼果蝇属叶芒绕眼果蝇复合种组研究(双翅目,果蝇科)

叶芒伏绕眼果蝇复合种组是Tsacas and Okada,1983建立的,已报告有6种,均出自于东洋区.记述了采自于中国及周边国家的伏绕眼果蝇属叶芒伏绕眼果蝇复合种组的10个种,并附有分种检索表.端尖叶芒伏绕眼果蝇Phortica foliacea(Tsacas et Okada,1983);叶芒伏绕眼果蝇Phortica foliiseta Duda,1923;侦测伏绕眼果蝇Phortica speculum(Máca et Lin,1993);异叶芒伏绕眼果蝇Phortica afoliolata Chen etToda,sp.nov.;短毛伏绕眼果蝇Phrtica brachychaeta Chen etToda,sP.nov.;拟叶芒伏绕眼果蝇Phortica foliisetoides Chenet Toda,sp.nov.;缺毛伏绕眼果蝇Phortica glabra Chen etToda,sp.nov.;刺伏绕眼果蝇Phortica spinosa Chen et Gao,sp.nov.;对称伏绕眼果蝇Phortica symmetria Chen et Toda,sp nov.;田边氏伏绕眼果蝇Phortica tanabei Chen et Toda,sp nov.     

Paramunnidae (Grustaea: Isopoda: Asellota) from the Magellan Strait

Seven new species of Paramunnidae from the Magellan Strait are described, two of which are placed in new genera: Paramunna magellanensis sp. nov., P. menziesi sp. nov., P. parasimplex sp. nov., P. patagoniensis sp. nov., Allorostrata ovalis gen. et sp. nov., Austrosignum dentatum sp. nov., and Magellianira serrata gen. et. sp. nov. Four known paramunnid species from the same location are redescribed: Parammunna integra, P. simplex, P. subtriangulata and Munnogonium tillerae . While investigating the genus Paramunna , two species of this group, Acutomunna foliacea (Chardy, 1975) gen. nov. and Paramunna foresti Carvacho , 1977 were removed, which involved the erection of a new genus. The allocation of P. foresti remains uncertain. Some diagnostic characters of the Paramunnidae and a key to the Magellanic species of Paramunna are presented.     

A Remarkable New Family of Jurassic Insects (Neuroptera) with Primitive Wing Venation and Its Phylogenetic Position in Neuropterida

Background

Lacewings (insect order Neuroptera), known in the fossil record since the Early Permian, were most diverse in the Mesozoic. A dramatic variety of forms ranged in that time from large butterfly-like Kalligrammatidae to minute two-winged Dipteromantispidae.

Principal Findings

We describe the intriguing new neuropteran family Parakseneuridae fam. nov. with three new genera and 15 new species from the Middle Jurassic of Daohugou (Inner Mongolia, China) and the Early/Middle Jurassic of Sai-Sagul (Kyrgyzstan): Parakseneura undula gen. et sp. nov., P. albomacula gen. et sp. nov., P. curvivenis gen. et sp. nov., P. nigromacula gen. et sp. nov., P. nigrolinea gen. et sp. nov., P. albadelta gen. et sp. nov., P. cavomaculata gen. et sp. nov., P. inflata gen. et sp. nov., P. metallica gen. et sp. nov., P. emarginata gen. et sp. nov., P. directa gen. et sp. nov., Pseudorapisma jurassicum gen. et sp. nov., P. angustipenne gen. et sp. nov., P. maculatum gen. et sp. nov. (Daohugou); Shuraboneura ovata gen. et sp. nov. (Sai-Sagul). The family comprises large neuropterans with most primitive wing venation in the order indicated by the presence of ScA and AA1+2, and the dichotomous branching of MP, CuA, CuP, AA3+4, AP1+2. The phylogenetic position of Parakseneuridae was investigated using a phylogenetic analysis of morphological scoring for 33 families of extinct and extant Neuropterida combined with DNA sequence data for representatives of all extant families. Parakseneuridae were recovered in a clade with Osmylopsychopidae, Prohemerobiidae, and Ithonidae.

Conclusions/Significance

The presence of the presumed AA1+2 in wings of Parakseneuridae is a unique plesiomorphic condition hitherto unknown in Neuropterida, the clade comprising Neuroptera, Megaloptera, Raphidioptera. The relative uncertainty of phylogenetic position of Parakseneuridae and the majority of other families of Neuroptera reflects deficient paleontological data, especially from critical important periods for the order, earliest Triassic and latest Triassic/earliest Jurassic.     

Neoselachian sharks and rays from the British Bathonian (Middle Jurassic)

Extensive sampling of Bathonian sediments from localities across southern and central England has produced over 8000 neoselachian teeth. These comprise diverse faunas, with over 25 species being represented in total, most of them previously undescribed. Seventeen new species and seven new genera are named: Palaeoscyllium tenuidens sp. nov., Praeproscyllium oxoniensis gen. et sp. nov., Eypea leesi gen. et sp. nov., Proheterodontus sylvestris gen. et sp. nov., Paracestracion bellis sp. nov., Palaeobrachaelurus mussetti sp. nov., Heterophorcynus microdon gen. et sp. nov., Dorsetoscyllium terraefullonicum gen. et sp. nov., Ornatoscyllium freemani gen. et sp. nov., Pseudonotidanus semirugosus gen. et sp. nov., Synechodus duffini sp. nov., Protospinax magnus sp. nov., P. bilobatus sp. nov., P. carvalhoi sp. nov., Belemnobatis kermacki sp. nov., B. stahli sp. nov. and Spathobatis delsatei sp. nov. In addition, a new family, the Pseudonotidanidae fam. nov., is defined and the status of Paranotidanus Ward and Thies, 1987, Hybodus levis Woodward, 1889 and Breviacanthus brevis (Phillips, 1871) are discussed. These taxa show strong facies specificity, with different species being restricted to different palaeoenvironments.     

EXCEPTIONALLY WELL-PRESERVED MIDDLE ORDOVICIAN SPONGES FROM THE LLANDEGLEY ROCKS LAGERSTÄTTE, WALES

Abstract:  A diverse fauna of three-dimensionally preserved sponges is described from nearshore volcanogenic sandstones near Llandrindod, Mid-Wales. The fauna was preserved through early marginal silicification, in rare examples with silicification of soft tissue, and includes aspicular and spicular demosponges, hexactinellids, and a heteractinid. The fauna is largely endemic, with the following new species, genera and families erected: Onerosiconcha gregalia gen. et sp. nov., Ordinisabulo quadragintaforma gen. et sp. nov., Miritubus erinaceus gen. et sp. nov., Vadosifistula milvus gen. et sp. nov., Polycornua trescelestus gen. et sp. nov. (Pseudolanciculidae fam. nov.), P. entropus sp. nov., Palaeocallyoides improbabilis gen. et sp. nov., Reticulicymbalum tres gen. et sp. nov., Triactinella rigbyi gen. et sp. nov. (Triactinellidae, fam. nov.), Spissiparies minuta gen. nov., Brevicirrus arenaceus gen. et sp. nov., Pyritonema scopula sp. nov. (Pyritonemidae, fam. nov.) and Microastraeum tenuis gen. et sp. nov. In addition, Pseudolancicula Webby and Trotter is recorded outside Australia for the first time, although only as isolated spicules. The environment represented by this fauna has not previously yielded articulated sponges, and thus the apparent endemism may be misleading.     

中国东北晚侏罗世虻类化石(昆虫纲:双翅目)

本文论述了辽宁西部上侏罗统虻类６个科的化石,包括１新科,１０个新属和１５个新种。其中Ｐａｌａｅｐａｎｇｏｎｉｕｓ ｅｕｐｔｅｒｕｓ ｇｅｎ．ｅｔ ｓｐ．ｎｏｖ．,Ｅｌｐａｎｇｏｎｉｕｓ ｐｌｅｔｕｓ ｇｅｎ,ｅｔ ｓｐ．ｎｏｖ．和Ａｌｌｏｍｙｉａ ｒｕｄｅｒａｌｉｓ ｇｅｎ．ｅｔ ｓｐ．ｎｏｖ．均属于虻科（Ｔａｂａｎｉｄａｅ）;Ｏｒｓｏｂｒａｃｈｙｃｅｒｏｎ ｃｈｉｎｅｎｓｉｓ ｇｅｎ．ｅｔ ｓｐ     

Salt stress affects sterol biosynthesis in the halophilic black yeast Hortaea werneckii

Hortaea werneckii is a black yeast recently isolated from salterns in Slovenia. Some of the adaptations of halophilic microorganisms to increased salinity and osmolarity of the environment are alterations in membrane properties. By modulating the fluidity, sterols play an important role as a component of eukaryotic biological membranes. We studied the regulation of sterol biosynthesis in H. werneckii through the activity and amount of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG R), a key regulatory enzyme in the biosynthesis of sterols. We found some differences in the characteristics of HMG R and in its regulation by different environmental salinities in H. werneckii when compared to the mesophilic baker's yeast, Saccharomyces cerevisiae. Our results suggest that halophilic black yeast regulates sterol biosynthesis through HMG R in a different way than mesophiles, which might be a consequence of the different ecophysiology of halophilic black yeasts. From this perspective, H. werneckii is an interesting novel model organism for studies on salt stress-responsive proteins as well as on sterol biosynthesis in eukaryotes.     

中国带织蛾属四新种和二新纪录种(鳞翅目:织蛾科)

记述了中国带织蛾属Periacma Meyrick 4新种：巍山带织蛾P.wershana sp.nov。,康定带织蛾P.kangdingensis sp.nov。,双叉带织蛾P.bifurcata sp.nov,和缺斑带织蛾P.immaculata sp.nov.,同时报道了2个中国新纪录种：暹罗带织蛾P.siamensis Moriuti,Saito et Lewvanich,安带织蛾P.angkhangensis Moriuti,Saito et Lewvanich,1985。提高了新种的雌雄外生殖器特征图,模式标本保存了南开大学生物学系。     

A new subfamily of ichneumonids from the Lower Cretaceous of Transbaikalia and Mongolia (Insecta: Hymenoptera: Ichneumonidae)

A new subfamily of ichneumonids, Palaeoichneumoninae, is described from the Lower Cretaceous of Transbaikalia (Baisa locality) and Mongolia (Bon Tsagan and Kholbotu Gol localities). The new subfamily is intermediate between the archaic subfamily Tanychorinae and the Recent Ichneumonidae. It includes 12 new species, described in three new genera: Palaeoichneumon freja gen. et sp. nov., P. ornatus sp. nov., P. danu sp. nov., P. micron sp. nov., P. mirabilis sp. nov., P. tenebrosus sp. nov., P. townesi sp. nov., Rudimentifera mora gen. et sp. nov., R. suspecta sp. nov., Dischysma maculata gen. et sp. nov., D. similis sp. nov., and D. ramulata sp. nov.     

Aspects of the progesterone response in Hortaea werneckii: Steroid detoxification, protein induction and remodelling of the cell wall

Progesterone in sublethal concentrations temporarily inhibits growth of Hortaea werneckii. This study investigates some of the compensatory mechanisms which are activated in the presence of progesterone and are most probably contributing to escape from growth inhibition. These mechanisms lead on the one hand to progesterone biotransformation/detoxification but, on the other, are suggested to increase the resistance of H. werneckii to the steroid. Biotransformation can detoxify progesterone efficiently in the early logarithmic phase, with mostly inducible steroid transforming enzymes, while progesterone biotransformation/detoxification in the late logarithmic and stationary phases of growth is not very efficient. The relative contribution of constitutive steroid transforming enzymes to progesterone biotransformation is increased in these latter phases of growth. In the presence of progesterone, activation of the cell wall integrity pathway is suggested by the overexpression of Pck2 which was detected in the stationary as well as the logarithmic phase of growth of the yeast. Progesterone treated H. werneckii cells were found to be more resistant to cell lysis than mock treated cells, indicating for the first time changes in the yeast cell wall as a result of treatment with progesterone.     

New Late Cretaceous Pollen Genera and Species in the Jiaag Han Basin of Hubei

In this paper, five new pollen genera and twenty new pollen species of angiospermae from the Late Cretaceous of a certain basin in central China are described. Four new organ genera and their species include: Morinoipollenites normalis gen. et sp. nov., Morinoipollenites minor gen. et sp. nov., Morinoipollenites polyprojectus gen. et sp. nov., Morinoipollenites rhombiformis gen. et sp. nov., Jianghanpollis ringens gen. et sp. nov., Jianghanpollis, mikros gen. et sp. nov., Jianghanpollis radiatus gen. et sp. nov., Jianghanpollis sayangensis gen. et sp. nov., Jianghanpollis arciformis gen. et sp. nov., Myoporumpollonites variabilis gen. et sp. nov., Myoporumpollenites striatus gen. et sp. nov., Lythraites giganteus Yu, Guo et Mao gen. et sp. nov. Lythraites clavatus sp. nov., Lythraites dilutus sp. nov., Lythraites magnus sp. nov., Lythraites varrustriatus sp. nov., Lythraites pachyrrhachis sp. nov.; the new-form genus and its species are: Crassimarginpollenites reticulatus gen. et sp. nov., Crassimerginpollenites mirus gen. et sp. nov., Crassimarginpollenites psilatus gen. et sp. nov. The new pollen genera and their species described herein have been compared in some details with recent pollens and considered their affinity with such recent families as Dipsacaceae, Myoporaceae, Apocynaceae and Lythraceae. According to the age of the pollen-bearing strata, a preliminary discussion is made regarding to the taxonomy of the recent plants families with which our new described pollens are related. Hence, this study may provide some mieropalaeobotanical information for deciphering 'the phylogeny of these plants.     

中国瘿绵蚜亚科Pemphiginae九新种记述(同翅目:瘿绵蚜科)(英文)

本文记述了中国瘿绵蚜科Pemphigidae瘿绵蚜亚科Pemphigidae9新种,草米根蚜MimeuriagramininiradicisZhangsp．nov,黑杨粗毛绵蚜PachypappaaigeirosZhangsp.nov,龙爪柳粗毛绵蚜P．trrtuosaeZhangsp.nov,少拟粗毛绵蚜PachypappaaigeirosZhangsp.nov,塔瘿绵蚜Pem-phigusturritusZhangsp.nov,武都瘿绵蚜P．wuduensisZhangSp．nov,金卷叶绵蚜Prociphilusau-rusZhangetQiaosp．nov,峨嵋卷叶绵蚜P．emeiensisZhangsp．nov．和三卷叶绵蚜P．trinusZhangspnov·。模式标本保存在中国科学院动物研究所。     

珀蝽属中国种类记述（半翅目：蝽科）

本文为珀蝽属中国种类的一次修订,共记述５个种和１个亚种,其中包括２个新种（邻珀蝽、黄珀蝽）和１个新亚种（庐山珀蝽云南亚种）。并对与中国区系有关的Ｐ．ｓｐｌｅｎｄｅｎｓ Ｄｉｓｔａｎｔ和Ｐ．ｖｉｒｉｄｉｃｏｌｌｉｓ模式标本作了重新描述。     

中国锦织蛾属的研究(鳞翅目 :织蛾科) :五新种和二新纪录种记述(英文)

本文记述了锦织蛾属五新种和中国二新纪录种。迄今为止 ,中国已知锦织蛾 33种。模式标本保存在南开大学生物系标本室。饰带锦织蛾Promalactisinfulata新种 .正模♂ ,河南信阳鸡公山 ,70 0m ,1997 Ⅶ 9,李后魂采 ;副模 3♀♀ ,1997 VII 11,其它采集资料同正模。本种可通过下列特征与该属其它已知种区别 :前翅表面斑纹较多 ;雄性外生殖器之抱器背短 ,阳茎角状器极长 ;雌性外生殖器之囊导管中部膨大。宝天曼锦织蛾Promalactisbaotianmanensis新种 .正模♂ ,河南内乡县宝天曼 ,1350m ,1998 Ⅶ 15,李后魂采 ;副模 15♀♀ ,1998 Ⅶ 13～ 15,其他采集资料同正模。本新种与PromalactispulchraWangetZheng外形相似 ,但可通过外生殖器特征与之区别 :新种雄性爪形突后缘中部凹入 ,两侧突出 ,阳茎端部无分支 ;雌性外生殖器导管端片后部呈三齿状。密纹锦织蛾Promalactisdensimacularis新种 .正模♂ ,江西井冈山小溪洞 ,1978 Ⅶ 7;副模 3♀♀ ,1978 VII 4～ 6,资料同正模。本种的前翅斑纹独特 ,雄性外生殖器颚形突端部两侧突出 ,形成不规则的突起 ,雌性外生殖器导管端片后缘呈三角形凹入等特征可与该属其它已知种区别。短瓣锦织蛾Promalactisbrevivalvaris新种 .正模♂ ,甘肃天水 ,1988 Ⅷ 10 ,武星煜采。本种与P     

A STUDY ON THE GENUS PROMALACTIS MEYRICK (LEPIDOPTERA:OECOPHORIDAE) FROM CHINA: FIVE NEW SPECIES AND TWO NEW RECORD SPECIES

Abstract  This paper deals with seven species of the genus Promalactis Meyrick from China. Among them, five species, i. e. P. infulata sp. nov., P. baotianmanensis sp. nov., P. densimacularis sp. nov., P. brevivalvaris sp. nov. and P. colacephala sp. nov., are described as new to science. Two species, P. bitaenia Park et Park and P. autoclina Meyrick, are recorded for the first time from this country. The genital structures of both sexes are illustrated.     

中国带织蛾属九新种和二新纪录种(鳞翅目:织蛾科)

记述了中国带织蛾属PeriacmaMeyrick 9新种 ,即曲靖带织蛾P .qujingensissp .nov .,离腹带织蛾P .absacculasp .nov .,新带织蛾P .novellasp .nov .,齿腹带织蛾P .sacculidenssp .nov .,三齿带织蛾P .tri dentatasp .nov .,尖爪带织蛾P .acriuncatasp .nov .,等瓣带织蛾P .equivalvatasp .nov .,尖颚带织蛾P .acutignathasp .nov .,中华带织蛾P .sinicasp .nov .。报道了中国 1新纪录亚种 ,越南带织蛾P .iodesmavietnamicaLvovsky ,1988和 1新纪录种伊带织蛾P .isanensisMoriuti,SaitoetLewvanich ,1989。提供了新种的雌雄外生殖器特征图。模式标本 (除指明外 )保存在南开大学生物学系。