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1.
Yasuhirou Ishimar Khurram Bashir Hiromi Nakanishi Naoko K Nishizawa 《Plant signaling & behavior》2011,6(10):1624-1626
Iron (Fe) is an essential micronutrient for plants whose deficiency presents a major worldwide agricultural problem. Moreover, Fe is not easily available in neutral to alkaline soils, rendering plants deficient in Fe despite its abundance. Plants secrete phenolics, such as protocatechuic acid (PCA) and caffeic acid (CA), to take up and utilize apoplasmic precipitated Fe, but despite the rapid progress in understanding cellular and subcellular Fe transport, the molecular mechanisms of phenolics synthesis and secretion are not clear. Recently, we isolated and characterized a phenolics efflux transporter in rice by characterizing a mutant in which the amount of PCA and CA in the xylem sap was dramatically reduced, which we hence named phenolics efflux zero 1 (pez1). PEZ1 is a plasma membrane protein that transports PCA when expressed in Xenopus laevis oocytes, and characterization of PEZ1 knockdown and overexpressing plants revealed that it plays an essential role in solubilizing precipitated apoplasmic Fe. The identification of PEZ1 will increase our understanding of apoplasmic Fe solubilization as well as promote research on phenolics efflux mechanisms in different organisms.Key words: iron, Oryza sativa, phenolics transport, protocatechuic acid, xylem sapAlthough mineral soils contain over 6% iron (Fe),1 it predominantly exists as Fe(III) chelates, and plants ultimately cannot absorb Fe under various physiological conditions such as high soil pH in alkaline soils.2 Thus, plants growing in high-pH soils are not very efficient in developing and stabilizing chlorophyll, resulting in the yellowing of leaves, poor growth and reduced yield. Plants, however, have developed sophisticated mechanisms to take up the small amount of soluble Fe. Non-graminaceous plants release protons, secrete phenolics, reduce Fe(III), and finally, take up Fe2+.3–5 Once Fe is solubilized, Fe(III) is reduced to Fe2+ by a membrane-bound Fe(III) reductase oxidase.6 Then Fe2+ is transported into the root by an iron-regulated transporter (IRT1). In contrast, graminaceous plants rely on an Fe(III) chelation system through the secretion of mugineic acid (MA) family phytosiderophores.3,7,8 The MAs are secreted to the rhizosphere through TOM1 9 and then they chelate Fe(III); the resulting Fe-MA complex is transported by the Yellow Stripe family transporters (OsYSL15 in the case of rice10). Rice plants also have the ability to take up Fe2+ through the OsIRT1 transporter.11In plants, Fe uptake from the apoplasm is well documented at the molecular level, with the exception of phenolics synthesis and efflux. Phenolics, such as protocatechuic acid (PCA), are reported to chelate Fe(III) solubilization and reduce it to Fe2+ in vitro.12 Moreover, removing the secreted phenolics in hydroponic culture solution triggers Fe deficiency responses in roots by inhibiting the solubilization and utilization of apoplasmic Fe.13 In this manner, phenolics play a major role in Fe solubilization, besides which PCA and other phenolics play a diverse role in biological systems, such as acting as antioxidants and free radical scavengers, and in nitric oxide synthase.14–17 Phenolics are also converted to lignin and suberin through the action of peroxidases.2 The activity of peroxidases, as well as the formation of lignin, decreases under Fe deficient conditions.2,18 As suberin plays an important role in controlling the movement of solutes,19 the role of phenolics in controlling water and mineral transport cannot be overlooked. Thus, understanding the molecular mechanism of phenolics efflux transport is crucial for developing strategies to mitigate widespread Fe deficiency.PEZ1 was isolated in an effort to characterize T-DNA mutants for genes regulated by cadmium (Cd). PEZ1 belongs to the multidrug and toxic compound extrusion transporter family whose members transport small organic compounds.20 The substrates of PEZ1 were identified by analyzing liquid chromatography/mass spectrometry data profiles of the xylem sap of pez1-1 and pez1-2 mutants. The data indicated that PEZ1 transports PCA and caffeic acid (CA). Furthermore, PEZ1 transported radiolabeled PCA when expressed in Xenopus laevis oocytes. PEZ1 localizes to the plasma membrane in rice root cells, as well as in rice root hairs and onion epidermal cells. The pez1-2 mutant accumulated more Fe in the roots, but not in the leaves, compared to wild-type (WT) plants; the differences were greater in the presence of Cd, while no difference was observed in the accumulation of other metals. No significant difference was observed in zinc, manganese (Mn), and copper concentration between WT and pez1-2, in both the roots and shoots, with or without Cd. Fe concentration in the xylem sap was lower than in the WT, while no difference was observed for xylem Cd and Mn. Significant differences in the localization of insoluble Fe were observed when leaf samples were stained with Perl''s solution to examine the localization of Fe. These results suggested a clear role of PEZ1 in solubilizing precipitated apoplasmic Fe.21Secretion of excess PCA strongly solubilizes Fe precipitated in the stele, leading to symptoms of Fe excess. The analysis of PEZ1 overexpression lines confirmed this hypothesis. PEZ1 overexpression lines accumulated higher amounts of Fe in roots and leaves owing to the high solubilization of precipitated apoplasmic stele Fe, and as a result, the growth of these lines was severely restricted. In contrast, PEZ1 overexpression lines grew better than the WT in calcareous soil, showing that in these lines, PCA-solubilized Fe is available under Fe-limiting conditions.The expression of PEZ1 is regulated by Cd, and both of the PEZ1 knockdown mutants accumulated higher Cd amounts in leaves and seeds when grown in soil, without compromising morphological or physiological characteristics, like the SPAD value, leaf dry weight, yield, and the concentration of other metals in seeds. Why pez1 accumulates Cd is not clear. PCA has a lower affinity for Cd compared to glutathione, and PEZ1 does not transport Cd.21 Cd is partly transported through the Fe uptake system in plants.22–26 Thus, in pez1, Cd accumulation seems to be triggered by the upregulation of OsIRT1. OsIRT1 localization in the phloem, its substrate specificity, and increased expression in pez1 mutants suggests that Fe and Cd uptake and translocation in pez1 mutants could be enhanced through OsIRT1,11 and that an increased Cd accumulation in pez1 mutants may be due to the increase in OsIRT activity in a decreased Fe environment in which Cd will have reduced competition. PEZ1 localizes to the stele in root cells. The localization of different genes involved in Fe transport is summarized in Figure 1.Open in a separate windowFigure 1Tissue-specific expression of Fe homeostasis-related genes in rice root.In short, phenolics secretion affects Fe acquisition in rice. Reduced secretion of PCA in the pez1-2 mutant impairs the solubilization of precipitated apoplasmic Fe in the stele, and thus, the low availability of Fe leads to the induction of OsIRT1. As PEZ1 and OsIRT1 co-localize in the stele, the PCA secretion may complement Fe2+ uptake by OsIRT1 and seems to be an integral part of the Fe2+ uptake system in rice (Fig. 2). In contrast, the increase in phenolics secretion in PEZ1-overexpressing plants increases the solubilization of apoplasmic Fe, and plants showed an increased tolerance to Fe deficiency in alkaline soils. The identification of PEZ1 is an important step that helps in better understanding the solubilization of apoplasmic Fe and will generate research on phenolics efflux mechanisms in other plants.Open in a separate windowFigure 2Model of Fe and Cd uptake mechanisms in rice xylem. P.M., plasma membrane. 相似文献
2.
Takeshi Kuroha Asako Okuda Masahiro Arai Yuta Komatsu Shusei Sato Tomohiko Kato Satoshi Tabata Shinobu Satoh 《Physiologia plantarum》2009,137(3):281-288
Xylem plays a role not only in the transport of water and nutrients but also in the regulation of growth and development through the transport of biologically active substances. In addition to mineral salts, xylem sap contains hormones, organic nutrients and proteins. However, the physiological functions of most of those substances remain unclear. To explore genes involved in xylem sap production, we identified Arabidopsis genes expressed in the root stele of the root hair zone from gene-trap lines by randomly inserting the β-glucuronidase gene into the genome. Among 26 000 gene-trap lines, we found that 10 lines had β-glucuronidase (GUS) staining predominantly in the root stele of the root hair zone and no GUS staining in the shoots. Of these 10 lines, 2 lines showed that gene-trap tags inserted into the promoter region of the same gene, denoted Arabidopsis thaliana subtilase 4.12( AtSBT4.12 ). Analysis of AtSBT4.12 promoter using an pAtSBT4.12 ::β-glucuronidase transgenic line showed that the AtSBT4.12 gene was expressed only in the root stele of the root hair zone. AtSBT4.12 expression in roots was increased by application of methyl jasmonate. Subtilase proteins are commonly detected in proteomic analyses of xylem sap from various plant species, including Brassica napus , a relative of Arabidopsis . These results suggest that AtSBT4.12 may be a protein localized in the apoplast of root stele including xylem vessel and involved in stress responses in Arabidopsis roots. 相似文献
3.
植物对硅的吸收转运机制研究进展 总被引:2,自引:0,他引:2
硅(Si)能缓解生物与非生物胁迫对植物的毒害作用,Si的吸收转运是由Si转运蛋白介导的.最近,多个Si转运蛋白(Lsi)基因相继在水稻、大麦和玉米中被克隆出来,并在Si的吸收转运机制方面取得了很大进展.水稻OsLsi在根组织中呈极性分布,OsLsi1定位在根外皮层和内皮层凯氏带细胞外侧质膜,负责将外部溶液中的单硅酸转运到皮层细胞内.OsLsi2定位在凯氏带细胞内侧质膜,在外皮层中负责将Si输出到通气组织质外体中,在内皮层与OsLsi1协同作用将Si转运到中柱中.导管中的Si通过蒸腾流转运到地上部,再由定位在叶鞘和叶片木质部薄壁细胞靠近导管一侧的OsLsi6负责木质部Si的卸载和分配.在大麦和玉米中,ZmLsi1/HvLsi1定位在根表皮和皮层细胞外侧质膜负责Si的吸收,然后Si通过共质体途径被转运到内皮层凯氏带细胞中,再由ZmLsi2/HvLsi2输出转运到中柱中.ZmLsi6在细胞中的定位和活性与OsLsi6相似,推测其可能具有类似的功能,但大麦Lsi6至今未见报道.所以,Si转运机制仍需要进一步研究. 相似文献
4.
OsFRDL1 Is a Citrate Transporter Required for Efficient Translocation of Iron in Rice 总被引:6,自引:0,他引:6
Kengo Yokosho Naoki Yamaji Daisei Ueno Namiki Mitani Jian Feng Ma 《Plant physiology》2009,149(1):297-305
Multidrug and toxic compound extrusion (MATE) transporters represent a large family in plants, but their functions are poorly understood. Here, we report the function of a rice (Oryza sativa) MATE gene (Os03g0216700, OsFRDL1), the closest homolog of barley (Hordeum vulgare) HvAACT1 (aluminum [Al]-activated citrate transporter 1), in terms of metal stress (iron [Fe] deficiency and Al toxicity). This gene was mainly expressed in the roots and the expression level was not affected by either Fe deficiency or Al toxicity. Knockout of this gene resulted in leaf chlorosis, lower leaf Fe concentration, higher accumulation of zinc and manganese concentration in the leaves, and precipitation of Fe in the root's stele. The concentration of citrate and ferric iron in the xylem sap was lower in the knockout line compared to the wild-type rice. Heterologous expression of OsFRDL1 in Xenopus oocytes showed transport activity for citrate. Immunostaining showed that OsFRDL1 was localized at the pericycle cells of the roots. On the other hand, there was no difference in the Al-induced secretion of citrate from the roots between the knockout line and the wild-type rice. Taken together, our results indicate that OsFRDL1 is a citrate transporter localized at the pericycle cells, which is necessary for efficient translocation of Fe to the shoot as a Fe-citrate complex. 相似文献
5.
The effects of iron deficiency on the composition of the xylem sap and leaf apoplastic fluid have been characterized in sugar beet (Beta vulgaris Monohil hybrid). pH was estimated from direct measurements in apoplastic fluid and xylem sap obtained by centrifugation and by fluorescence of leaves incubated with 5-carboxyfluorescein and fluorescein isothiocyanate-dextran. Iron deficiency caused a slight decrease in the pH of the leaf apoplast (from 6.3 down to 5.9) and xylem sap (from 6.0 down to 5.7) of sugar beet. Major organic acids found in leaf apoplastic fluid and xylem sap were malate and citrate. Total organic acid concentration in control plants was 4.3 mM in apoplastic fluid and 9.4 mM in xylem sap and increased to 12.2 and 50.4 mM, respectively, in iron-deficient plants. Inorganic cation and anion concentrations also changed with iron deficiency both in apoplastic fluid and xylem sap. Iron decreased with iron deficiency from 5.5 to 2.5 microM in apoplastic fluid and xylem sap. Major predicted iron species in both compartments were [FeCitOH](-1) in the controls and [FeCit(2)](-3) in the iron-deficient plants. Data suggest the existence of an influx of organic acids from the roots to the leaves via xylem, probably associated to an anaplerotic carbon dioxide fixation by roots. 相似文献
6.
Microbes detoxify arsenate by reduction and efflux of arsenite. Plants have a high capacity to reduce arsenate, but arsenic efflux has not been reported. Tomato (Lycopersicon esculentum) and rice (Oryza sativa) were grown hydroponically and supplied with 10 microm arsenate or arsenite, with or without phosphate, for 1-3 d. The chemical species of As in nutrient solutions, roots and xylem sap were monitored, roles of microbes and root exudates in As transformation were investigated and efflux of As species from tomato roots was determined. Arsenite remained stable in the nutrient solution, whereas arsenate was rapidly reduced to arsenite. Microbes and root exudates contributed little to the reduction of external arsenate. Arsenite was the predominant species in roots and xylem sap. Phosphate inhibited arsenate uptake and the appearance of arsenite in the nutrient solution, but the reduction was near complete in 24 h in both -P- and +P-treated tomato. Phosphate had a greater effect in rice than tomato. Efflux of both arsenite and arsenate was observed; the former was inhibited and the latter enhanced by the metabolic inhibitor carbonylcyanide m-chlorophenylhydrazone. Tomato and rice roots rapidly reduce arsenate to arsenite, some of which is actively effluxed to the medium. The study reveals a new aspect of As metabolism in plants. 相似文献
7.
Based on experimental data obtained in earlier studies on membranepermeabilities of abscisic acid (ABA) for cortex and stele cellsof roots and on measured com-partmental pH shifts after onsetor release of different types of soil-borne stresses, a biomathematicalmodel was developed which permits computer analysis of the dynamicsof compartmental ABA distribution within different root tissues(cortex, stele) and their compartments (apoplast, cytosol, vacuole),and in the xylem sap of the root stele. Metabolism and conjugationof ABA and its export from roots via the xylem and its importinto roots via phloem sap flow are also taken into consideration.We want to know which soil-borne stresses can biophysicallyprovoke a root-to-shoot signal of ABA. In this communicationwe describe the biomathematical structure of the root modeland present all necessary morphological (volumes, surfaces etc.)and physiological (pH, membrane conductances etc.) parametersof unstressed roots. This root model and an available leaf modelare integrated to a plant model (rosette plant). Simulationsreveal the fundamental role of the stele tissues, the rhizosphericABA concentration and the ABA synthesis in roots (root-to-shootcommunication). The shoot-to-root communication strongly dependson ABA synthesis in leaves, but hardly on ABA redistributioneffects after stress-induced compartmental pH-shifts in leaves. Key words: Abscisic acid, compartmental redistribution, computer model, pH shifts, root-to-shoot communication, shoot-to-root communication 相似文献
8.
Highly efficient xylem transport of arsenite in the arsenic hyperaccumulator Pteris vittata 总被引:3,自引:0,他引:3
The hyperaccumulator Pteris vittata translocates arsenic (As) from roots to fronds efficiently, but the form of As translocated in xylem and the main location of arsenate reduction have not been resolved. Here, P. vittata was exposed to 5 microM arsenate or arsenite for 1-24 h, with or without 100 microM phosphate. Arsenic speciation was determined in xylem sap, roots, fronds and nutrient solutions by high-performance liquid chromatography (HPLC) linked to inductively coupled plasma mass spectrometry (ICP-MS). The xylem sap As concentration was 18-73 times that in the nutrient solution. In both arsenate- and arsenite-treated plants, arsenite was the predominant species in the xylem sap, accounting for 93-98% of the total As. A portion of arsenate taken up by roots (30-40% of root As) was reduced to arsenite rapidly. The majority (c. 80%) of As in fronds was arsenite. Phosphate inhibited arsenate uptake, but not As translocation. More As was translocated to fronds in the arsenite-treated than in the arsenate-treated plants. There was little arsenite efflux from roots to the external solution. Roots are the main location of arsenate reduction in P. vittata. Arsenite is highly mobile in xylem transport, possibly because of efficient xylem loading, little complexation with thiols in roots, and little efflux to the external medium. 相似文献
9.
Yaping Zhou Benjamin Neuhäuser Günter Neumann Uwe Ludewig 《Plant, cell & environment》2020,43(7):1691-1706
Under phosphorus (P) deficiency, Lupinus albus (white lupin) releases large amounts of organic acid anions from specialized root structures, so-called cluster or proteoid roots, to mobilize and acquire sparingly soluble phosphates from a restricted soil volume. The molecular mechanisms underlying this release and its regulation are, however, poorly understood. Here, we identified a gene belonging to the aluminium (Al)-activated malate transporter (ALMT) family that specifically contributes to malate, but not citrate release. This gene, LaALMT1, was most prominently expressed in the root apices under P deficiency, including those of cluster roots and was also detected in the root stele. Contrary to several ALMT homologs in other species, the expression was not stimulated, but moderately repressed by Al. Aluminium-independent malate currents were recorded from the plasma membrane localized LaALMT1 expressed in Xenopus oocytes. In composite lupins with transgenic roots, LaALMT1 was efficiently mutated by CRISPR-Cas9, leading to diminished malate efflux and lower xylem sap malate concentrations. When grown in an alkaline P-deficient soil, mutant shoot phosphate concentrations were similar, but iron and potassium concentrations were diminished in old leaves, suggesting a role for ALMT1 in metal root to shoot translocation, a function that was also supported by growth in hydroponics. 相似文献
10.
11.
Rice is efficient at arsenic (As) accumulation, thus posing a potential health risk to humans and animals. Arsenic bioavailability in submerged paddy soil is enhanced due to mobilisation of arsenite, but rice may also have an inherently greater ability to take up and translocate arsenite than other cereal crops. To test this hypothesis, rice, wheat and barley were exposed to 5 µM arsenate or arsenite for 24 h. Arsenic uptake and distribution, and As speciation in the xylem sap and nutrient solution were determined. Regardless of the As form supplied to plants, rice accumulated more As in the shoots than wheat or barley. Arsenite uptake by rice was double of that by wheat or barley, whereas arsenate uptake was similar between rice and wheat and approximately a third smaller in barley. The efficiency of As translocation from roots to shoots was greater when plants were supplied with arsenite than with arsenate, and in both treatments rice showed the highest translocation efficiency. Arsenite was the main species of As (86–97%) in the xylem sap from arsenite-treated plants of all three species. In the arsenate-treated plants, 84%, 45% and 63% of As in the xylem sap of rice, wheat and barley, respectively, was arsenite. Arsenite efflux to the external medium was also observed in all three plant species exposed to arsenate. The results show that rice is more efficient than wheat or barley in arsenite uptake and translocation, probably through the highly efficient pathway for silicon. 相似文献
12.
Organic acids and Fe deficiency: a review 总被引:4,自引:2,他引:2
Abadía Javier López-Millán Ana-Flor Rombolà Adamo Abadía Anunciación 《Plant and Soil》2002,246(1):75-86
Organic acid concentrations often increase with iron deficiency in different plant parts such as roots, leaves and stem exudates. The review summarises data available on the changes in the concentrations of organic anions in plants with iron deficiency and the effects of these changes in plant metabolism. The paper reviews data available in the literature on the changes in xylem and apoplasmic fluid composition with iron deficiency, both in plants grown in controlled conditions and in the field, and discusses the possible ways of iron complexation and transport in these compartments. The characteristics of the iron reduction and uptake by the iron-deficient leaf mesophyll cells are also discussed, with especial emphasis in the possible roles of organic acids in these processes. Both the possible causes and functions of the organic acid concentration increases in iron-deficient plants are reviewed. 相似文献
13.
Export of Abscisic Acid, 1-Aminocyclopropane-1-Carboxylic Acid,Phosphate, and Nitrate from Roots to Shoots of Flooded Tomato Plants (Accounting for Effects of Xylem Sap Flow Rate on Concentration and Delivery) 总被引:10,自引:1,他引:9
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We determined whether root stress alters the output of physiologically active messages passing from roots to shoots in the transpiration stream. Concentrations were not good measures of output. This was because changes in volume flow of xylem sap caused either by sampling procedures or by effects of root stress on rates of whole-plant transpiration modified concentrations simply by dilution. Thus, delivery rate (concentration x sap flow rate) was preferred to concentration as a measure of solute output from roots. To demonstrate these points, 1-aminocyclopropane-1-carboxylic acid (ACC), abscisic acid, phosphate, nitrate, and pH were measured in xylem sap of flooded and well-drained tomato (Lycopersicon esculentum Mill., cv Ailsa Craig) plants expressed at various rates from pressurized detopped roots. Concentrations decreased as sap flow rates were increased. However, dilution of solutes was often less than proportional to flow, especially in flooded plants. Thus, sap flowing through detopped roots at whole-plant transpiration rates was used to estimate solute delivery rates in intact plants. On this basis, delivery of ACC from roots to shoots was 3.1-fold greater in plants flooded for 24 h than in well-drained plants, and delivery of phosphate was 2.3-fold greater. Delivery rates of abscisic acid and nitrate in flooded plants were only 11 and 7%, respectively, of those in well-drained plants. 相似文献
14.
Phytosiderophore efflux transporters are crucial for iron acquisition in graminaceous plants 总被引:8,自引:0,他引:8
Nozoye T Nagasaka S Kobayashi T Takahashi M Sato Y Sato Y Uozumi N Nakanishi H Nishizawa NK 《The Journal of biological chemistry》2011,286(7):5446-5454
Eukaryotic organisms have developed diverse mechanisms for the acquisition of iron, which is required for their survival. Graminaceous plants use a chelation strategy. They secrete phytosiderophore compounds, which solubilize iron in the soil, and then take up the resulting iron-phytosiderophore complexes. Bacteria and mammals also secrete siderophores to acquire iron. Although phytosiderophore secretion is crucial for plant growth, its molecular mechanism remains unknown. Here, we show that the efflux of deoxymugineic acid, the primary phytosiderophore from rice and barley, involves the TOM1 and HvTOM1 genes, respectively. Xenopus laevis oocytes expressing TOM1 or HvTOM1 released (14)C-labeled deoxymugineic acid but not (14)C-labeled nicotianamine, a structural analog and biosynthetic precursor of deoxymugineic acid, indicating that the TOM1 and HvTOM1 proteins are the phytosiderophore efflux transporters. Under conditions of iron deficiency, rice and barley roots express high levels of TOM1 and HvTOM1, respectively, and the overexpression of these genes increased tolerance to iron deficiency. In rice roots, the efficiency of deoxymugineic acid secretion was enhanced by overexpression of TOM1 and decreased by its repression, providing further evidence that TOM1 encodes the efflux transporter of deoxymugineic acid. We have also identified two genes encoding efflux transporters of nicotianamine, ENA1 and ENA2. Our identification of phytosiderophore efflux transporters has revealed the final piece in the molecular machinery of iron acquisition in graminaceous plants. 相似文献
15.
Effect of nitrate on water transfer across roots of nitrogen pre-starved maize seedlings 总被引:8,自引:0,他引:8
Jackson Hoarau Laure Barthes Agnès Bousser Eliane Deléens Jean-Louis Prioul 《Planta》1996,200(4):405-415
The addition of 10 mM KNO3 to the solution bathing the roots of young nitrogen-starved seedlings of Zea mays L. enhanced root water transfer within 15 h, compared with 10 mM KCl addition. The free exudation flux was 2.2–3.9 times higher in excised KNO3-treated roots than in KCl-treated ones. Cryo-osmometry data for xylem sap suggested that, compared with chloride, nitrate treatment increased the steady solute flux into the xylem, but did not modify the osmotic concentration of sap. Root growth was not significantly modified by nitrate within 15 h. Root hydraulic conductances were measured by using either hydrostatic-pressure or osmotic-gradient methods. During hydrostatic experiments, the conductance (kp), which is thought to refer mainly to the apoplasmic pathway, was 1.6 times larger in KNO3-than in KCl-treated plants. From experiments in which polyethylene glycol (PEG) 8000 was used as external osmolyte, osmotic conductances (ks) were found to be smaller by 5–20 times than kp for the two kinds of plants. The KCl-treated roots were characterized by a low ks which was the same for influx or efflux of water. By contrast, KNO3-treated roots exhibited two distinct conductances ks1 and ks2, indicating that influx of water was easier than efflux when the water flow was driven by the osmotic pressure gradient. Infiltration of roots with KNO3 solution supported the idea that nitrate might enhance the efficiency of the cell-to-cell pathway. The low ks value of KCl-treated roots and the existence of two contrasting ks values (ks1 and ks2) for KNO3-treated roots are discussed in terms of reversible closing of water channels. 相似文献
16.
Hayashi Hiroaki Okada Yoshihisa Mano Hironori Kume Tamikazu Matsuhashi Shinpei S-Ishioka Noriko Uchida Hiroshi Chino Mitsuo 《Plant and Soil》1997,196(2):233-237
Nitrogen movement through the xylem vessels and sieve tubes in rice plants was studied using xylem and phloem sap analysis in combination with stable and radioactive nitrogen isotope techniques.More than 90% of nitrogen was translocated in the sieve tubes of rice plants as amino acids. When 15N (99.6 atom%) was applied as a nitrate to the root, 15N first appeared in phloem sap of the leaf sheath within 10 minutes and increased to 37 atom% excess 5 hours after the experiment had started. In long-term experiments, 63% of nitrogen in the phloem sap of the leaf sheath and 15% in that of the uppermost internode came from nitrogen absorbed within the last 24 hours and 50 hours, respectively.To obtain information about the more rapid circulation of nitrogen in the plant, radioactive 13N was used as a tracer. A positron-emitting tracer imaging system was used to show that 13N was transferred to the leaf sheath within 8 minutes of its application to the roots. Analysis of the xylem sap of the leaf sheath showed that when the nitrate was applied to the roots, most of the nitrogen in the xylem was transported as a nitrate.These data showed that phloem and xylem sap analysis together with the stable and radioactive nitrogen techniques provide a good method for the detection of nitrogen cycles in plants. 相似文献
17.
In higher plants, the xylem vessels functionally connect the roots with the above-ground organs. The xylem sap transports various organic compounds, such as proteins and amino acids. We examined drought and rewatering-inducible changes in the amino acid composition of root xylem sap collected from Cucurbita maxima roots. The major free amino acids in C . maxima root xylem sap were methylglycine (MeGly; sarcosine) and glutamine (Gln), but MeGly was not detected in the xylem sap of cucumber. MeGly is an intermediate compound in the metabolism of trimethylglycine (TMG; betaine), but its physiological effects in plants are unknown. Drought and rewatering treatment resulted in an increase in the concentration of MeGly in root xylem sap to 2.5 m M . After flowering, the MeGly concentration in the xylem sap dropped significantly, whereas the concentration of Gln decreased only after fruit ripening. One milli molar MeGly inhibited the formation of adventitious roots and their elongation in C . maxima , but glycine, dimethylglycine, or TMG had no effect. Similar effects and the inhibition of stem elongation were observed in shoot cuttings of cucumber and Phaseolus angularis . These observations seem to imply a possible involvement of xylem sap MeGly in the physiological responses of C . maxima plants to drought stress. 相似文献
18.
Here we have shown that 24 hours after addition of zeatin to the nutrient solution the cytokinin content in xylem sap of wheat plants appears to be about 10 times lower that in the nutrient solution. Cytokinins accumulated mostly in roots and not in shoots of treated plants. These data demonstrate the existence of some barrier on cytokinin pathway from the nutrient solution to the plant shoot. With the help of Sudan III an increase in lignin and suberin deposition in the endodermis could be detected, being stronger with the increase in the distance from the root tip. The increase in deposition of suberin and lignin coincided with the decrease in cytokinin immunolabeling in root cells revealed with the help of monoclonal cytokinin antibodies and the second gold-labelled antibodies. Simultaneously exogenous cytokinins accumulated in root stele cells showing that the Casparian band was not only barrier on cytokinin pathway to plant shoot. It is concluded that high cytokinin immunolabe ling in the stele parenchyma cells around the stele vessels demonstrated accumulation of cytokinins by these cells, which could be important in regulation of cytokinin loading to the xylem vessels during there transport to the shoot. The role of cytokinin transporters is discussed. 相似文献
19.
Plants produce compounds in roots that are transported to shoots via the xylem sap. Some of these compounds are vital for signalling and adaptation to environmental stress such as drought. In this study, we screened the xylem sap using mass spectrometry to quantify the changes in new and previously identified sap constituents under extended drought. We detected and quantified the changes in the concentration of 31 compounds present in the xylem sap under progressively increasing drought stress. We found changes in the hormones abscisic acid (ABA) and cytokinin, and the presence of high concentrations of the aromatic cytokinin 6-benzylaminopurine (BAP). Several phenylpropanoid compounds (coumaric, caffeic and ferulic acids) were found in xylem sap. The concentrations of some of these phenylpropanoid compounds changed under drought. In parallel, an analysis of the xylem sap proteome was conducted. We found a higher abundance of cationic peroxidases, which with the increase in phenylpropanoids may lead to a reduction in lignin biosynthesis in the xylem vessels and could induce cell wall stiffening. The application of new methodologies provides insights into the range of compounds in sap and how alterations in composition may lead to changes in development and signalling during adaptation to drought. 相似文献
20.
Orera I Rodríguez-Castrillón JA Moldovan M García-Alonso JI Abadía A Abadía J Alvarez-Fernández A 《Metallomics : integrated biometal science》2010,2(9):646-657
A dual-stable isotope tracer experiment was carried out with Fe-deficient sugar beet plants grown hydroponically and resupplied with differentially Fe labeled racemic and meso Fe(iii)-chelates of the ethylendiamine di(o-hydroxyphenylacetic) acid (o,oEDDHA). No short-term Fe isotope exchange reactions occurred in the nutrient solution and plants did not discriminate between (54)Fe and (57)Fe. After 3-6 h, stable Fe isotopes, chelating agents and chelates were analyzed in roots, xylem sap and leaves by ICP-MS and HPLC-ESI/TOFMS. Ferric chelate reductase rates, xylem transport and total uptake were 2-fold higher with the meso isomer than with the racemic one. Both chelating agent isomers were incorporated and distributed by plants at similar rates, in amounts one order of magnitude lower than those of Fe. After 6 h of Fe resupply, most of the Fe acquired was localized in roots, whereas most of the chelating agent was in leaves. In a separate experiment, Fe-deficient sugar beet and tomato plants were treated with different concentrations of Fe(iii)-o,oEDDHA (with a meso/racemic ratio of 1). The xylem sap Fe concentration at 24 h was unaffected by the chelate concentration, with xylem Fe(iii)-o,oEDDHA accounting for 1-18% of total Fe and xylem meso/racemic ratio close to 1. Although most of the Fe coming from Fe(iii)-o,oEDDHA was taken up through a reductive dissociative mechanism, a small part of the Fe may be taken up via non-dissociative mechanisms. 相似文献