共查询到20条相似文献,搜索用时 15 毫秒
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Adeno-associated virus-delivered polycistronic microRNA-clusters for knockdown of vascular endothelial growth factor in vivo 总被引:1,自引:0,他引:1
Pihlmann M Askou AL Aagaard L Bruun GH Svalgaard JD Holm-Nielsen MH Dagnaes-Hansen F Bek T Mikkelsen JG Jensen TG Corydon TJ 《The journal of gene medicine》2012,14(5):328-338
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Trafficking of adeno‐associated virus vectors across a model of the blood–brain barrier; a comparative study of transcytosis and transduction using primary human brain endothelial cells 下载免费PDF全文
Steven F. Merkel Allison M. Andrews Evan M. Lutton Dakai Mu Eloise Hudry Bradley T. Hyman Casey A. Maguire Servio H. Ramirez 《Journal of neurochemistry》2017,140(2):216-230
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Assessment of brain metabolite correlates of adeno‐associated virus‐mediated over‐expression of human alpha‐synuclein in cortical neurons by in vivo 1H‐MR spectroscopy at 9.4 T 下载免费PDF全文
Sandra Cuellar‐Baena Natalie Landeck Sarah Sonnay Kerstin Buck Vladimir Mlynarik René in ‘t Zandt Deniz Kirik 《Journal of neurochemistry》2016,137(5):806-819
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The dual effect of ultrasound‐targeted microbubble destruction in mediating recombinant adeno‐associated virus delivery in renal cell carcinoma: transfection enhancement and tumor inhibition 下载免费PDF全文
Fan Li Lifang Jin Huiping Wang Fang Wei Min Bai Qiusheng Shi Lianfang Du 《The journal of gene medicine》2014,16(1-2):28-39
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A novel bispecific molecule delivered by recombinant AAV2 suppresses ocular inflammation and choroidal neovascularization 下载免费PDF全文
Yiming Li Ping Zhu Amrisha Verma Tuhina Prasad Hongxin Deng Dechao Yu Qiuhong Li 《Journal of cellular and molecular medicine》2017,21(8):1555-1571
Elevated vascular endothelial growth factor (VEGF) and complement activation are implicated in the pathogenesis of different ocular diseases. The objective of this study was to investigate the hypothesis that dual inhibition of both VEGF and complement activation would confer better protection against ocular inflammation and neovascularization. In this study, we engineered a secreted chimeric VEGF inhibitor domain (VID), a complement inhibitor domain (CID) and a dual inhibitor (ACVP1). Vectors expressing these three inhibitors were constructed and packaged into AAV2 (sextY‐F) particles. The expression and secretion of the proteins were validated by Western blot. The effects of these inhibitors expressed from AAV2 vectors were examined in endotoxin‐induced uveitis (EIU), experimental autoimmune uveoretinitis (EAU) and choroidal neovascularization (CNV) mouse models. The AAV2 vectors expressing the CID‐ and ACVP1‐attenuated inflammation in EIU and EAU model, whereas the vector expressing VID showed improved retinal structure damaged by EAU, but not affect the infiltration of inflammatory cells in EAU or EIU eyes. Both VID and CID vectors improved laser‐induced retinal and choroid/RPE injuries and CNV, whereas ACVP1 vector provided significantly better protection. Our results suggest that gene therapy targeting VEGF and complement components could provide an innovative and long‐term strategy for ocular inflammatory and neovascular diseases. 相似文献
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Gene therapy for Glut1‐deficient mouse using an adeno‐associated virus vector with the human intrinsic GLUT1 promoter 下载免费PDF全文
Sachie Nakamura Shin‐ichi Muramatsu Naomi Takino Mika Ito Eriko F. Jimbo Kuniko Shimazaki Tatsushi Onaka Sumio Ohtsuki Tetsuya Terasaki Takanori Yamagata Hitoshi Osaka 《The journal of gene medicine》2018,20(4)
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Adeno‐associated virus 8‐mediated gene therapy for choroideremia: preclinical studies in in vitro and in vivo models 下载免费PDF全文
Aaron Black Vidyullatha Vasireddy Daniel C. Chung Albert M. Maguire Rajashekhar Gaddameedi Tania Tolmachova Miguel Seabra Jean Bennett 《The journal of gene medicine》2014,16(5-6):122-130
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Ting He Nataliya Smith Debra Saunders Sabrina Doblas Yasuko Watanabe Jessica Hoyle Robert Silasi‐Mansat Florea Lupu Megan Lerner Daniel J. Brackett Rheal A. Towner 《Journal of cellular and molecular medicine》2011,15(4):837-849
Angiogenesis is essential to tumour progression and a precise evaluation of angiogenesis is important for tumour early diagnosis and treatment. The quantitative and dynamic in vivo assessment of tumour angiogenesis can be achieved by molecular magnetic resonance imaging (mMRI). Vascular endothelial growth factor (VEGF) and VEGF receptors (VEGFRs) are the main regulatory systems in angiogenesis and have been used as hot targets for radionuclide‐based molecular imaging. However, little research has been accomplished in targeting VEGF/VEGFRs by mMRI. In our study, we aimed to assess the expression of VEGFR2 in C6 gliomas by using a specific molecular probe with mMRI. The differential uptake of the probe conjugated to anti‐VEGFR2 monoclonal antibody, shown by varied increases in T1 signal intensity during a 2 hr period, demonstrated the heterogeneous expression of VEGFR2 in different tumour regions. Microscopic fluorescence imaging, obtained for the biotin group in the probe with streptavidin‐Cy3, along with staining for cellular VEGFR2 levels, laminin and CD45, confirmed the differential distribution of the probe which targeted VEGFR2 on endothelial cells. The angiogenesis process was also assessed using magnetic resonance angiography, which quantified tumour blood volume and provided a macroscopic view and a dynamic change of the correlation between tumour vasculature and VEGFR2 expression. Together these results suggest mMRI can be very useful in assessing and characterizing the expression of specific angiogenic markers in vivo and help evaluate angiogenesis associated with tumour progression. 相似文献
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Chester Bittencourt Sacramento Flavia Helena da Silva Nance Beyer Nardi Eduardo Gallatti Yasumura José Carlos Costa Baptista‐Silva Abram Beutel Ruy Ribeiro de Campos Jane Zveiter de Moraes Hamilton Silva Junior Vivian Yochiko Samoto Radovan Borojevic Sang Won Han 《The journal of gene medicine》2010,12(3):310-319