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1.
Christopher C. Payne Peter P.C. Mertens Kazumasa Katagiri 《Journal of invertebrate pathology》1978,32(3):310-318
A cytoplasmic polyhedrosis virus (CPV) from the pine caterpillar, Dendrolimus spectabilis, was compared with Japanese isolates of closely related viruses from the silkworm, Bombyx mori, and gypsy moth, Lymantria dispar. The sizes of the viral RNA genome segments were almost identical, although the CPVs from D. spectabilis and L. dispar could be distinguished from the silkworm virus by a small size difference (0.03 × 106 daltons) in one segment. The same viruses were also distinguishable by RNA homology differences of 25–50% measured by the reannealing of 3H-labeled single-stranded viral messenger RNA (synthesized in vitro) to heat-denatured viral double-stranded RNA. Antigenic differences were also detected by gel immunodiffusion tests. CPVs of D. spectabilis and L. dispar were indistinguishable by these criteria. 相似文献
2.
Comparative studies were performed on the replication of the Autographa californica nuclear polyhedrosis virus in cell lines from Estigmene acrea, BTI-EAA; Lymantria dispar, IPLB-LD64BA; Mamestra brassicae, IZD-MB0503; Spodoptera frugiperda, IPLB-SF1254; and Trichoplusia ni, TN-368. Significant differences were observed in the amount of virus obtained from the cell lines, with M. brassicae and T. ni producing more polyhedra than the other lines. These two cell lines also produced nonoccluded virus most rapidly, followed by S. frugiperda, E. acrea, and L. dispar. Sensitivities of the cell lines to infection by the virus, as determined by plaque formation, followed the same pattern, with M. brassicae being most sensitive and L. dispar least so. The T. ni cell line produced polyhedra which were more pathogenic to T. ni larvae than those from the other cells. These differences have important implications in the application of cell cultures in the development of microbial insecticides. 相似文献
3.
John A. Couch 《Journal of invertebrate pathology》1974,24(3):311-331
A nuclear polyhedrosis virus exists in pink shrimp, Penaeus duorarum, from waters of the northern Gulf of Mexico. This virus is rod-shaped, 269 nm long, and possesses an outer envelope surrounding its nucleocapsid. The nucleocapsid is 50 nm in diameter. The virus occurs in nuclei of host hepatopancreatic and midgut cells, and is both free in the nucleus and occluded within pyramidal-shaped polyhedral inclusion bodies (PIB's). Histochemically and ultrastructurally, the shrimp PIB's appear to be ribonucleoprotein and in fine structure bear close resemblance to polyhedral inclusion bodies of Baculovirus species from insects. However, the lattice line-to-line spacing is greater than that usually reported for insect PIB's. Crowding and chemical stress of shrimp in aquaria may enhance and increase the virus infection and prevalence. In limited experiments, shrimp fed heavily infected hepatopancreatic tissues had much higher mortality than controls fed only fish. The virus appears to be enzootic in pink shrimp in nature. Cytopathological changes in infected cells of shrimp appear similar to those in insects infected with certain species of Baculovirus. The name Baculovirus penaei n.sp. is proposed for the shrimp virus. 相似文献
4.
S. Tvermyr 《BioControl》1969,14(3):245-250
Larvae ofNeodiprion sertifer (Geoff.) were reared on twigs from Scots pine at three different temperatures, 12°C, 18°C and 24°C. The LT50 of virus-infected larvae was 19.3, 9.5 and 4,6 days respectively. In the control the median length of the larval period was 45.7, 29.8 and 22.1 days at the same temperatures. From this results it is concluded that both the LT50 and the length of the larval period are prolonged by low temperature. The medium length of the larval period, however, is relatively more prolonged than is the LT50. Therefore, nuclear polyhedrossis virus may be an effective control agent againstN. sertifer in cool areas even if the LT50 is relatively long. 相似文献
5.
In the late 1960's the degree of safety testing required of new candidate pesticides reached a climax. During this period, the nuclear polyhedrosis virus (NPV) ofHeliothis zea (Boddie) underwent a series of tests as thorough as those required for chemicals by the Environmental Protection Agency (E.P.A.) in the U.S.A. and by guidelines recommended by W.H.O. These included long term carcinogenicity and teratogenicity tests, tests on primates and tests on man. Indeed, the tests were far more demanding than the tests for chemicals because they examined the possibility of infection of test animals by the insect viruses. They led to the registration of a pioneer viral insecticide containing this NPV produced in caterpillars. Two other products from Lepidoptera, containing NPVs ofOrgyia pseudotsugata (McDunn) andLymantria dispar L. have satisfied the E.P.A. registration requirements. The NPV ofNeodiprion sertifer (Geoffr.) (Hym.) has proved harmless in extensive tests, including long term tests. Another 3 NPVs, those ofAutographa californica (Speyer)Spodoptera littoralis Boisd. andS. exempta (Walk.) passed tests not including the long term tests. Also a non-occluded baculovirus of a coleopteran,Oryctes rhinoceros L., has passed extensive pathogenicity tests and tests in cell lines. A number of other NPVs have been partially tested and limited tests have been made on 2 granulosis viruses (GV). The NPVs proved harmless to—and unable to replicate in—microorganisms, non-insect invertebrate cell lines vertebrate cell lines, vertebrates, plants and non-arthropod invertebrates. Replication was unusual in insects outside the insect family in which the virus was first found. GVs occur only in Lepidoptera, most are believed to be very specific and none have replicated in cell lines from insects or other animals. In addition, the rapidly expanding discipline of Invertebrate Pathology has failed to find incidence of NPVs and GVs infecting hosts outside the above stated host ranges. This is in reality a vast body of evidence matched only in extent by the absence of incidence of NPVs and GVs from the publications of medical, veterinary and phytopathology science. This evidence, and the accrued data from specific safety testing, gives increasing confidence that individual NPVs and GVs of Lepidoptera and Hymenoptera are very specific. This confidence suggests that new NPVs and GVs in these orders need be subjected only to a reduced range of the more challenging tests and to tests designed to reveal harm originating from the insect species used for virus production and from contaminants. 相似文献
6.
After solubilization of polyhedra of Autographa californica, Lymantria dispar, and Mamestra brassicae nuclear polyhedrosis viruses, PAGE showed at least eight distinct polyhedral polypeptide bands. Whereas the molecular weights of the major polypeptide were similar for the three NPVs (28.0–30.0 kdalton), characteristic differences between the species were found for the minor polypeptides having molecular weights in the range from 12.4 to 62.0 kdalton. It is assumed that these polypeptides are not generated by polyhedral alkaline protease since they are detected after protease inactivation. The data demonstrate that different baculoviruses can be distinguished from each other by SDS-PAGE of their polyhedral polypeptides. 相似文献
7.
Median lethal doses (LD50s) of nuclear polyhedrosis virus (NPV) were determined in neonatal offspring ofSpodoptera frugiperda (J. E. Smith) (Sf) larvae captured in southeastern Louisiana in 1981, 1982, and 1984. These LD50s ranged from 1.8 to 16.3 polyhedral inclusion bodies (PIB)/insect. The LD50s significantly (P<0.05) increased during the season of 1982 but had no pattern in 1981 or 1984. However, the Sf populations increased in heterogeneity of response to the NPV during all 3 years. The LD50 increased from 4.1 to 18.7 PIB/insect in a Sf laboratory colony exposed to the NPV LD80 for 7 generations, whereas in a control colony not exposed to NPV the LD50 was 5.9 PIB/insect after 7 generations. 相似文献
8.
Three cell lines of Heliothis zea and one cell line of Heliothis virescens replicated the singleembedded, nuclear polyhedrosis virus (NPV) of H. Zea, (i.e., Baculovirus heliothis) with concomitant production of polyhedral inclusion bodies (PIB). Between 20 and 60% of the H. zea cells produced PIB, whereas only 3% of H. virescens cells were found to produce PIB. The H. zea cell lines produced 10 to 20 times more PIB than did the H. virescens cell line. The PIB from all cell lines produced typical symptoms of an NPV infection when bioassayed against larvae of H. zea. More than 99% of the total viral activity of the final whole culture was due to the PIB. 相似文献
9.
The insect baculovirus AcMNPV (Autographa californica multiple nuclear polyhedrosis virus) enters many mammalian cell lines, prompting its application as a general eukaryotic gene delivery agent, but the basis of entry is poorly understood. For adherent mammalian cells, we show that entry is favoured by low pH and by increasing the available cell-surface area through a transient release from the substratum. Low pH also stimulated baculovirus entry into mammalian cells grown in suspension which, optimally, could reach 90% of the transduced population. The basic loop, residues 268–281, of the viral surface glycoprotein gp64 was required for entry and a tetra mutant with increasing basicity increased entry into a range of mammalian cells. The same mutant failed to plaque in Sf9 cells, instead showing individual cell entry and minimal cell-to-cell spread, consistent with an altered fusion phenotype. Viruses grown in different insect cells showed different mammalian cell entry efficiencies, suggesting that additional factors also govern entry. 相似文献
10.
Tarcísio Fontenele de Brito Vitor Lima Coelho Maira Arruda Cardoso Ingrid Alexandre de Abreu Brito Mateus Antonio Berni Fides Lea Zenk Nicola Iovino Attilio Pane 《PLoS pathogens》2021,17(8)
Triatomine assassin bugs comprise hematophagous insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. Although the microbiome of these species has been investigated to some extent, only one virus infecting Triatoma infestans has been identified to date. Here, we describe for the first time seven (+) single-strand RNA viruses (RpV1-7) infecting Rhodnius prolixus, a primary vector of Chagas disease in Central and South America. We show that the RpVs belong to the Iflaviridae, Permutotetraviridae and Solemoviridae and are vertically transmitted from the mothers to the progeny via transovarial transmission. Consistent with this, all the RpVs, except RpV2 that is related to the entomopathogenic Slow bee paralysis virus, established persistent infections in our R. prolixus colony. Furthermore, we show that R. prolixus ovaries express 22-nucleotide viral siRNAs (vsiRNAs), but not viral piRNAs, that originate from the processing of dsRNA intermediates during viral replication of the RpVs. Interestingly, the permutotetraviruses and sobemoviruses display shared pools of vsiRNAs that might provide the basis for a cross-immunity system. The vsiRNAs are maternally deposited in the eggs, where they likely contribute to reduce the viral load and protect the developing embryos. Our results unveil for the first time a complex core virome in R. prolixus and begin to shed light on the RNAi-based antiviral defenses in triatomines. 相似文献
11.
In vivo and in vitro host range of Autographa californica nuclear polyhedrosis virus and Spodoptera frugiperda nuclear polyhedrosis virus 总被引:2,自引:0,他引:2
Baculoviruses from Autographa californica (AcNPV-E2) and Spodoptera frugiperda (SfNPV-2) were titered in five insect cell lines: IAL-PID2, IAL-SFD1, IPLB-SF-21AE, TN-368, and IAL-TND1. AcNPV-E2 replicated in all the cell lines while SfNPV-2 did not replicate in the lines TN-368 and IAL-TND1. Further in vivo studies of SfNPV-2 showed the virus was not infectious when fed to Trichoplusia ni larvae per os or when injected into the hemocoel. These data suggest that the barrier to SfNPV-2 infectivity in T. ni is at the cellular level, as opposed to the midgut. 相似文献
12.
C.F. Reichelderfer 《Journal of invertebrate pathology》1974,23(1):46-50
The 100,000g supernatant from an alkaline dissolution of polyhedra isolated from an NPV of Spodoptera frugiperda was found to agglutinate adult chicken erythrocytes in a pH range of 5.5–6.9. Optimal conditions for active hemagglutination and hemagglutination-inhibition, with antisera prepared against polyhedron protein, occurred at pH 5.8 with an incubation temperature of 37°C and a solublization time of 45 min at pH 11.2 Minimum quantities of antigen detectable were at 2–4 μg/ml of protein. 相似文献
13.
Viral particles of the nuclear polyhedrosis virus (Baculovirus) of the gypsy moth, Porthetria dispar, appear to be released from hemocyte nuclei by budding through both inner and outer lamellae of the nuclear envelope. As a result of budding, the virus particle acquires its envelope from the inner lamella of the nuclear envelope. The outer lamella, which forms a membrane-limited vesicle around the enveloped particles, may fuse with the plasma membrane during viral release from host cells by exocytosis. These observations differ from two other reported cases of nuclear budding in NPV-infected cells in that the process occurred in the absence of nuclear inclusion bodies. 相似文献
14.
Terry Fei Fan Ng Rachel Marine Chunlin Wang Peter Simmonds Beatrix Kapusinszky Ladaporn Bodhidatta Bamidele Soji Oderinde K. Eric Wommack Eric Delwart 《Journal of virology》2012,86(22):12161-12175
Deep sequencing of untreated sewage provides an opportunity to monitor enteric infections in large populations and for high-throughput viral discovery. A metagenomics analysis of purified viral particles in untreated sewage from the United States (San Francisco, CA), Nigeria (Maiduguri), Thailand (Bangkok), and Nepal (Kathmandu) revealed sequences related to 29 eukaryotic viral families infecting vertebrates, invertebrates, and plants (BLASTx E score, <10−4), including known pathogens (>90% protein identities) in numerous viral families infecting humans (Adenoviridae, Astroviridae, Caliciviridae, Hepeviridae, Parvoviridae, Picornaviridae, Picobirnaviridae, and Reoviridae), plants (Alphaflexiviridae, Betaflexiviridae, Partitiviridae, Sobemovirus, Secoviridae, Tombusviridae, Tymoviridae, Virgaviridae), and insects (Dicistroviridae, Nodaviridae, and Parvoviridae). The full and partial genomes of a novel kobuvirus, salivirus, and sapovirus are described. A novel astrovirus (casa astrovirus) basal to those infecting mammals and birds, potentially representing a third astrovirus genus, was partially characterized. Potential new genera and families of viruses distantly related to members of the single-stranded RNA picorna-like virus superfamily were genetically characterized and named Picalivirus, Secalivirus, Hepelivirus, Nedicistrovirus, Cadicistrovirus, and Niflavirus. Phylogenetic analysis placed these highly divergent genomes near the root of the picorna-like virus superfamily, with possible vertebrate, plant, or arthropod hosts inferred from nucleotide composition analysis. Circular DNA genomes distantly related to the plant-infecting Geminiviridae family were named Baminivirus, Nimivirus, and Niminivirus. These results highlight the utility of analyzing sewage to monitor shedding of viral pathogens and the high viral diversity found in this common pollutant and provide genetic information to facilitate future studies of these newly characterized viruses. 相似文献
15.
Immunodiffusion and tube precipitation tests, polyacrylamide gel electrophoresis of virus polypeptides, and cross-transmission experiments suggest that two nuclear polyhedrosis viruses, one from Lymantria monacha and one from L. dispar, are partially related to each other, but not identical. The virus particle proteins seem to be more specific than the polyhedron proteins. 相似文献
16.
The serological relationships of five nuclear polyhedrosis viruses (NPV) were investigated using the immunodiffusion technique with intragel absorption. Reciprocal tests demonstrated that virion fractions from Autographa californica multiple embedded virus (MEV), Heliothis armigera MEV, and H. zea single embedded virus (SEV) are not related to each other or to virions from Trichoplusia ni SEV and Pseudoplusia includens SEV. Virion fractions of T. ni and P. includens NPV were shown to be closely related, sharing several antigens. Matrix fractions possessed a common group antigen and one or two antigens specific for the individual NPV with the exception that T. ni and P. includens NPV shared one of these antigens. The specific antigens of the matrix fraction were also shared with the homologous virion fraction. 相似文献
17.
Eight species of noctuid larvae were tested for susceptibility to a nuclear polyhedrosis virus of the velvetbean caterpillar, Anticarsia gemmatalis. Velvetbean caterpillar larvae were highly susceptible to crude preparations of polyhedral inclusion bodies (PIBs; LD50 = 4.7 PIBs/larva), but preparations of purified polyhedra were much less effective against these larvae (LD50 = 319.7 PIBs/larva). Of seven other noctuid species tested, only Heliothis virescens was as susceptible to the virus as A. gemmatalis. High dosages were required to kill Heliothis zea, Trichoplusia ni, Pseudoplusia includens, and Spodoptera ornithogalli. Plathypena scabra and Spodoptera frugiperda were not susceptible. 相似文献
18.
This is the first report of plaque formation by a pathogenic insect virus. Trichoplusia ni (TN-368) cells overlaid with medium containing 0.6% methyl cellulose continued to multiply, developed into monolayers, and produced plaques after infection with alfalfa looper nuclear polyhedrosis virus. Viral polyhedral inclusion bodies were first observed 24 hr after exposure of cells to virus, and plaques continued to increase in size for 72 hr. Two different types of plaques were observed: one in which all cells had many polyhedra in their nuclei, and another in which few cells had inclusion bodies. When virus from either plaque was injected into T. ni larvae, they died of typical nuclear polyhedrosis virus disease. The assay was reproducible, and plaque numbers were related to virus concentration. 相似文献
19.
Y. K. Choe S. M. Byun H. H. Lee T. W. Chung H. Y. Park S. K. Kang 《Biotechnology letters》1986,8(12):853-858
Summary A nuclear polyhedrosis virus ofHyphantria
cunea replicated successfully in theTrichoplusia
ni cell line. Restriction endonuclease analysis of the viral DNA obtained from infected cell culture showed the same general homogeneity as that from virus isolated from diseased host larvae. Electron microscopic observations showed that the occluded virus from cell culture consists of rod-like nucleocapsids (31×320 nm) enveloped in aggregates and embedded in polyhedral inclusion bodies from 0.6 to 2.5 m in diameter. 相似文献
20.
D.T. Briese H.A. Mende T.D.C. Grace P.W. Geier 《Journal of invertebrate pathology》1980,36(2):211-215
Measurements were made of the relative susceptibility to a nuclear polyhedrosis virus of three populations of light-brown apple moth Epiphyas postvittana: a resistant laboratory strain (CAN), a susceptible laboratory strain (BAR), and a field population. CAN was found to be 50 times more resistant than BAR and 160 times more resistant than the field line. Experiments on hybrid crosses of resistant and susceptible strains showed that resistance is genetically determined. This serves as a warning of the possible selection of virus-resistant strains of insect pests, where viral insecticides are being used in the field. 相似文献