共查询到20条相似文献,搜索用时 15 毫秒
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Martin-Magniette ML Aubert J Cabannes E Daudin JJ 《Bioinformatics (Oxford, England)》2005,21(9):1995-2000
MOTIVATION: In cDNA microarray experiments all samples are labeled with either Cy3 or Cy5. Systematic and gene-specific dye bias effects have been observed in dual-color experiments. In contrast to systematic effects which can be corrected by a normalization method, the gene-specific dye bias is not completely suppressed and may alter the conclusions about the differentially expressed genes. METHODS: The gene-specific dye bias is taken into account using an analysis of variance model. We propose an index, named label bias index, to measure the gene-specific dye bias. It requires at least two self-self hybridization cDNA microarrays. RESULTS: After lowess normalization we have found that the gene-specific dye bias is the major source of experimental variability between replicates. The ratio (R/G) may exceed 2. As a consequence false positive genes may be found in direct comparison without dye-swap. The stability of this artifact and its consequences on gene variance and on direct or indirect comparisons are addressed. AVAILABILITY: http://www.inapg.inra.fr/ens_rech/mathinfo/recherche/mathematique 相似文献
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Differential calmodulin gene expression in the rodent brain 总被引:4,自引:0,他引:4
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High-throughput phenomics: experimental methods for mapping fluxomes 总被引:21,自引:0,他引:21
Sauer U 《Current opinion in biotechnology》2004,15(1):58-63