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1.
Korol'kov V.I. Platonova G.A. Azanova V.V. Tennikova T.B. Vlasov G.P. 《International journal of peptide research and therapeutics》2000,7(1):53-61
Summary The preparation method of peptide ligands employing polymer-supported solid-phase synthesis and leading to biospecific sorbents
has been designed and optimized. This approach directly affords porous polymer sorbents for biospecific chromatography and
avoids the cleavage of the synthesized peptide moieties from the carrier and their isolation. The specifics of both peptide
synthesis and biospecific chromatography using hydrophilic macroporous polymer supports based on porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) beads and discs were also investigated. The protecting groups can be removed from the target peptide
(bradykinin) attached to the polymer support by trifluoromethylsulfonic acid without any significant loss of the attached
peptide from the polymer carrier. Introduction of styrene as a comonomer into the copolymer structure improves the reactivity
of the support. However, no nonspecific adsorption of proteins in the course of the biospecific isolation of antibradykinin
antibodies was observed with these media. In contrast, the nonspecific sorption of proteins increases as a result of increasing
peptide loading. 相似文献
2.
We present a new concept of synthesis for preparation of molecularly imprinted polymers using a functionalized initiator to replace the traditional functional monomer. Using propranolol as a model template, a carboxyl-functionalized radical initiator was demonstrated to lead to high-selectivity polymer particles prepared in a standard precipitation polymerization system. When a single enantiomer of propranolol was used as template, the imprinted polymer particles exhibited clear chiral selectivity in an equilibrium binding experiment. Unlike the previous molecular imprinting systems where the active free radicals can be distant from the template-functional monomer complex, the method reported in this work makes sure that the actual radical polymerization takes place in the vicinity of the template-associated functional groups. The success of using functional initiator to synthesize molecularly imprinted polymers brings in new possibilities to improve the functional performance of molecularly imprinted synthetic receptors. 相似文献
3.
R V Devivar S L Koontz W J Peltier J E Pearson T A Guillory J D Fabricant 《Bioorganic & medicinal chemistry letters》1999,9(9):1239-1242
An alternative solid support for oligonucleotide synthesis was developed by coupling a polymer colloid to a modified polyethylene filter disc. The functions on the polymer colloid not used for attachment to the surface were derivatized with a Jeffamine diamine and loaded with appropriate deoxynucleoside succinates. The performance of this support system was evaluated and compared to existing resins. 相似文献
4.
Wang W Li L Jin C Niu Y Li S Ma J Li L Liu Y Cai L Zhao W Wang PG 《Bioorganic & medicinal chemistry letters》2011,21(17):5041-5044
The Letter describes a strategy for the enzymatic synthesis of glycans based on a pH-responsive water-soluble polymer. In neutral condition, the polymer is water-soluble and convenient for in-solution enzymatic synthesis, whereas in acidic condition (pH lower than 4.0), the polymer disconnects with the product and becomes insoluble, which can be easily removed. A 6-Sialyl lactose analogue was synthesized as a model reaction using this approach. 相似文献
5.
Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications. 相似文献
6.
International Cooperation for Metal Recycling From Waste Electrical and Electronic Equipment 总被引:1,自引:0,他引:1
Andreas Manhart 《Journal of Industrial Ecology》2011,15(1):13-30
This article addresses a market‐based management concept for waste electrical and electronic equipment (WEEE) known as the “best‐of‐two‐worlds” approach. The concept is based on the idea that recyclers in developing countries and emerging economies can cooperate with technologically advanced refineries in industrialized countries to facilitate efficient recovery of valuable metals, such as gold and palladium, from e‐waste. The article provides an overview of technical and environmental concerns underlying the concept and sheds light on the political framework, the waste‐related trade issues, and the resource economics that need to be considered for further decision making. Building on this synthesis, I conduct a qualitative assessment of sustainability impacts of the proposed concept by analyzing two scenarios and their associated risks. The analysis suggests that, under certain preconditions, the best‐of‐two‐worlds concept could yield significant improvements in terms of management of hazardous substances, resource efficiency, greenhouse gas emissions, income generation, and investments into social and environmental standards. Generally, two potential implementation scenarios were identified: Whereas under Scenario 1 only WEEE generated within developing countries and emerging economies is managed through the best‐of‐two‐worlds approach, Scenario 2 additionally incorporates WEEE imported from industrialized countries. Although both scenarios can yield a variety of benefits, Scenario 2 might cause a net flow of hazardous substances from industrialized countries into developing countries and emerging economies, thus leading to less beneficial sustainability impacts. 相似文献
7.
Surawski PP Battersby BJ Lawrie GA Ford K Rühmann A Marcon L Kozak D Trau M 《Molecular bioSystems》2008,4(7):774-778
The concept of optically encoding particles for solid phase organic synthesis has existed in the literature for several years. However, there remains a significant challenge to producing particles that are capable of withstanding harsh solvents and reagents whilst maintaining the integrity and range of the optical encoding. In this study, a new generation of fluorescently encoded support particles was used for both solid phase peptide synthesis and on-particle analysis of proteolysis in a multiplexed, flow cytometric assay. The success of the assay was demonstrated through the use of a model protease, trypsin. Our results show that the use of solid supports with high peptide yield, high swellability in water and high penetration of the enzyme into the interior of the particle is not absolutely necessary for proteolysis assays. 相似文献
8.
Christina Uth Simon Englert Olga Avrutina Harald Kolmar Sascha Knauer 《Journal of peptide science》2023,29(12):e3527
We report the first application of a novel amino-Li resin to water-based solid-phase peptide synthesis (SPPS) applying the Smoc-protecting group approach. We demonstrated that it is a suitable support for the sustainable water-based alternative to a classical SPPS approach. The resin possesses good swelling properties in aqueous milieu, provides significant coupling sites, and may be applicable to the synthesis of difficult sequences and aggregation-prone peptides. 相似文献
9.
The biological functions of a variety of proteins are regulated by heparan sulfate glycosaminoglycans. In order to facilitate the elucidation of the molecular basis of glycosaminoglycan-protein interactions we have developed syntheses of heparin-like oligosaccharides on polymer supports. A completely stereoselective strategy previously developed by us for the synthesis of these oligosaccharides in solution has been extended to the solid phase using an acceptor-bound approach. Both a soluble polymer support and a polyethylene glycol-grafted polystyrene resin have been used and different strategies for the attachment of the acceptor to the support have been explored. The attachment of fully protected disaccharide building blocks to a soluble support through the carboxylic group of the uronic acid unit by a succinic ester linkage, the use of trichloroacetimidates as glycosylating agents and of a functionalized Merryfield type resin for the capping process allowed for the construction of hexasaccharide and octasaccharide fragments containing the structural motif of the regular region of heparin. This strategy may facilitate the synthesis of glycosaminoglycan oligosaccharides by using the required building blocks in the glycosylation sequence. 相似文献
10.
Direct rapid synthesis of MIP beads in SPE cartridges 总被引:1,自引:0,他引:1
Selecting optimal compositions for non-covalent molecularly imprinted polymers (MIPs) and screening for appropriate rebinding conditions necessitates synthesising a large number of polymers. This is extremely labour-intensive and usually results in very limited "optimisation" in studies of MIPs. Here, a new method is proposed for rapid synthesis of MIPs in a beaded form that can be used directly in many different performance evaluation studies. The method is based on synthesis of spherical particles by suspension polymerisation in liquid fluorocarbon [Mayes, A., Mosbach, K., 1996. Molecularly imprinted polymer beads: suspension polymerisation using a liquid perfluorocarbon as the dispersing phase. Anal. Chem. 68, 3769-3774]. The polymers were directly polymerised under UV light in solid phase extraction (SPE) cartridges, then washed and extracted in the same cartridges where they had been synthesised, resulting in a rapid and automatable process that requires no transfer or manipulation of the polymer particles. The particles were similar in terms of size, morphology and functional performance to particles obtained by suspension polymerisation in fluorocarbon solvent using a conventional reactor. In this initial study, 36 polymers were synthesised to study the effect of a variation in the type and amount of four different functional monomers, methacrylic acid (MAA), acrylic acid (AA), hydroxyethyl methacrylate (HEMA) and 2-vinylpyridine (2-VPy), for the imprinting of propranolol and morphine. The performance of polymers synthesised using MAA was as expected, but those synthesised with AA as functional monomer showed more surprising rebinding properties as a function of monomer to cross-linker ratios, demonstrating the potential value of pragmatic synthesis and screening approaches to polymer optimisation. 相似文献
11.
P. Kumar A. K. Sharma P. Sharma B. S. Garg K. C. Gupta 《Nucleosides, nucleotides & nucleic acids》2013,32(4):879-888
Abstract A rapid and general one-pot method is described for the attachment of the leader nucleoside onto the polymer supports, suitable for polymer supported oligonucleotide synthesis following oxidation-reduction condensation. The method can also be used for support functionalisation in fully automated DNA synthesizer prior to oligonucleotide synthesis. 相似文献
12.
Application of new catalytic phosphate protecting groups for the highly efficient phosphotriester oligonucleotide synthesis. 总被引:1,自引:1,他引:0
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V A Efimov A A Buryakova I Y Dubey N N Polushin O G Chakhmakhcheva Ovchinnikov YuA 《Nucleic acids research》1986,14(16):6525-6540
An effective procedure for the synthesis of oligonucleotides by the phosphotriester method has been developed. The procedure is based on the use of phosphate protecting groups enabling O-nucleophilic intramolecular catalysis in the reaction of internucleotide bond formation under the action of arylsulfonyl chlorides and their derivatives. Using this new procedure, the time needed to perform one elongation step on polymer support is 7-8 min. The effectiveness of the methodology has been demonstrated in the synthesis of many oligodeoxyribonucleotides of different length with high yields. 相似文献
13.
V.I. Korol''kov G.A. Platonova V.V. Azanova T.B. Tennikova G.P. Vlasov 《Letters in Peptide Science》2000,7(1):53-61
The preparation method of peptide ligandsemploying polymer-supported solid-phase synthesisand leading to biospecific sorbents has beendesigned and optimized. This approach directlyaffords porous polymer sorbents for biospecificchromatography and avoids the cleavage of thesynthesized peptide moieties from the carrier andtheir isolation. The specifics of both peptidesynthesis and biospecific chromatography usinghydrophilic macroporous polymer supports based onporous poly(glycidyl methacrylate-co-ethylenedimethacrylate) beads and discs were alsoinvestigated. The protecting groups can be removed from the target peptide (bradykinin) attached tothe polymer support by trifluoromethylsulfonic acidwithout any significant loss of the attached peptidefrom the polymer carrier. Introduction of styreneas a comonomer into the copolymer structureimproves the reactivity of the support. However, nononspecific adsorption of proteins in the course ofthe biospecific isolation of antibradykininantibodies was observed with these media. Incontrast, the nonspecific sorption of proteinsincreases as a result of increasing peptide loading. 相似文献
14.
A novel combinatorial approach to synthesize oligonucleotides on fluorescently encoded microspheres based on flow sorting and segmental solid-phase synthesis is described. BODIPY dyes were covalently attached to polystyrene (8.8 microm, 55% DVB) microsphere particles to generate four fluorescently encoded sets. 20-mer oligonucleotide sequences can be synthesized on these microspheres with yields comparable to conventional CPG supports (80% overall yield, average stepwise yield = 99%). The concept of segmental solid-phase synthesis by flow sorting was demonstrated by synthesizing unique 20-mer oligonucleotide sequences on each of four fluorescently encoded microsphere sets by including a flow sorting step (after first eight base additions) and flow cytometric detection of sequences synthesized on each microsphere set by hybridization with fluorescently labeled complementary sequence. 相似文献
15.
There is a growing need in membrane separations for novel membrane materials providing selective retention. Molecularly imprinted polymers (MIPs) are promising candidates for membrane functionalization. In this work, a novel approach is described to prepare composite membrane adsorbers incorporating molecularly imprinted microparticles or nanoparticles into commercially available macroporous filtration membranes. The polymerization is carried out in highly viscous polymerization solvents, and the particles are formed in situ in the pores of the support membrane. MIP particle composite membranes selective for terbutylazine were prepared and characterized by scanning electron microscopy and N? porosimetry. By varying the polymerization solvent microparticles or nanoparticles with diameters ranging from several hundred nanometers to 1 μm could be embedded into the support. The permeability of the membranes was in the range of 1000 to 20,000 Lm?2 hr?1 bar?1. The imprinted composite membranes showed high MIP/NIP (nonimprinted polymer) selectivity for the template in organic media both in equilibrium-rebinding measurements and in filtration experiments. The solid phase extraction of a mixture of the template, its analogs, and a nonrelated compound demonstrated MIP/NIP selectivity and substance selectivity of the new molecularly imprinted membrane. The synthesis technique offers a potential for the cost-effective production of selective membrane adsorbers with high capacity and high throughput. 相似文献
16.
Injectable polymer particles with a diameter in the range of 30-300 microm find applications as a biomaterial in different clinical fields, such as cosmetic surgery, reconstructive surgery, and urology. However, clinical effects tend to disappear after several months, either due to migration of the particles away from the injection site (caused by weak adherence with the surrounding soft tissues) or due to fibrosis (caused by excessive encapsulation of the particles by fibrous tissue). Little is known about the fate of injected microparticles, due to the fact that they are extremely difficult to trace in a noninvasive manner. Design, synthesis, and characterization of new polymeric microspheres with two additional features that can enhance safety and can help to overcome drawbacks of existing products are reported. First, the new microparticles feature clear radio-opacity (X-ray visibility) as they are prepared on the basis of a reactive methacrylic monomer that contains covalently bound iodine. Model experiments reveal that the level of X-ray contrast is sufficient for clinical monitoring; they can be visualized both during the injection and afterward. The particles feature excellent cytocompatibility in vitro and in vivo. Second, a method is explored to functionalize the surface of the particles, for example, through immobilization of collagen. Other extracellular matrix proteins can also be immobilized, and this provides a mechanism to control anchoring of the particles in soft tissue. The results are briefly discussed in the context of improved biomaterials, contemporary X-ray imaging, and control over biomaterial-soft tissue interactions in vivo. 相似文献
17.
We report here a direct surface-grafting approach to forming DNA-containing polymer shells outside of Au nanoparticles using aqueous atom transfer radical polymerization (ATRP). In this approach, DNA molecules were immobilized on Au particles to introduce ATRP initiators on the surface. The same DNA molecules also acted as particle stabilizers through electrostatic repulsion and allowed particles to stay suspended in water. The immobilized ATRP initiators prompted polymer chain growth under certain conditions to form thick polymer shells outside of the particles. The formation of DNA-polymer hybrids outside of Au nanoparticles was characterized using absorption spectroscopy, dynamic light scattering (DLS), transmission electron microscopy (TEM), and gel electrophoresis. The presence of thick polymer shells improved particle stability in high ionic strength media, whereas particles with the DNA coating only aggregated. A visible color difference between these two particle solutions was clearly observed, providing the basis for DNA sensing in homogeneous solutions. 相似文献
18.
Mitchell AR 《Biopolymers》2008,90(3):175-184
Bruce Merrifield, trained as a biochemist, had to address three major challenges related to the development and acceptance of solid-phase peptide synthesis (SPPS). The challenges were (1) to reduce the concept of peptide synthesis on a insoluble support to practice, (2) overcome the resistance of synthetic chemists to this novel approach, and (3) establish that a biochemist had the scientific credentials to effect the proposed revolutionary change in chemical synthesis. How these challenges were met is discussed in this article. 相似文献
19.
Berlanda Scorza F Colucci AM Maggiore L Sanzone S Rossi O Ferlenghi I Pesce I Caboni M Norais N Di Cioccio V Saul A Gerke C 《PloS one》2012,7(6):e35616
Gram-negative bacteria naturally shed particles that consist of outer membrane lipids, outer membrane proteins, and soluble periplasmic components. These particles have been proposed for use as vaccines but the yield has been problematic. We developed a high yielding production process of genetically derived outer membrane particles from the human pathogen Shigella sonnei. Yields of approximately 100 milligrams of membrane-associated proteins per liter of fermentation were obtained from cultures of S. sonnei ΔtolR ΔgalU at optical densities of 30-45 in a 5 L fermenter. Proteomic analysis of the purified particles showed the preparation to primarily contain predicted outer membrane and periplasmic proteins. These were highly immunogenic in mice. The production of these outer membrane particles from high density cultivation of bacteria supports the feasibility of scaling up this approach as an affordable manufacturing process. Furthermore, we demonstrate the feasibility of using this process with other genetic manipulations e.g. abolition of O antigen synthesis and modification of the lipopolysaccharide structure in order to modify the immunogenicity or reactogenicity of the particles. This work provides the basis for a large scale manufacturing process of Generalized Modules of Membrane Antigens (GMMA) for production of vaccines from gram-negative bacteria. 相似文献
20.
In this article, we report on the facile and rapid synthesis of conjugation polymer poly(p-phenylenediamine) nanobelts (PNs) via room temperature chemical oxidation polymerization of p-phenylenediamine monomers by ammonium persulfate in aqueous medium. We further demonstrate the proof-of-concept that PNs can be used as an effective fluorescent sensing platform for nucleic acid detection for the first time. The general concept used in this approach lies in the facts that the adsorption of the fluorescently labeled single-stranded DNA probe by PN leads to substantial fluorescence quenching, followed by specific hybridization with the complementary region of the target DNA sequence. This results in desorption of the hybridized complex from PN surface and subsequent recovery of fluorescence. We also show that the sensing platform described herein can be used for multiplexing detection of nucleic acid sequences. 相似文献