Benthic macrophyte metrics as bioindicators of water quality: towards overcoming typological boundaries and methodological tradition in Mediterranean and Black Seas

Benthic macrophyte communities of different substratum types (soft, hard) were studied in eleven differently impacted sites belonging in two different water typologies (transitional waters: Lesina Lagoon, Varna Lake; coastal waters: Varna Bay) and two ecoregions (Mediterranean Sea, Black Sea). Species lists were compiled for each study site, 20 taxa were found at Lesina and Varna Lake and Bay, and the abundance of each taxon was determined at each site. The relationship between nine metrics related to community structure [species richness, % of total coverage, dry biomass (g/m^?2), and cluster and multi-dimensional scaling plot of Bray–Curtis similarity] and function [Ecological Status Group I % coverage, ESG II % coverage, Ecological Evaluation Index (EEI-c) and Ecological Index (EI_EEI)] and key abiotic factors and an anthropogenic stress index (EnII) were studied. A strong relationship (Spearman rank correlation coefficient ρ ≤ ?0.89; R ² ≥ 0.89) between anthropogenic stress and functional indices, EEI-c and EI_EEI, was found. The structural index ‘species richness’ correlated negatively with EnII and positively with salinity, demonstrating a freshwater and confinement influence on species diversity. EEI-c and EI_EEI indices classified the studied sites and locations in different Ecological Status Classes in accordance with the anthropogenic stress gradient.     

The growth of pikeperch (Sander lucioperca L.) and perch (Perca fluviatilis L.) under different water temperature and salinity conditions in the Curonian Lagoon and Lithuanian coastal waters of the Baltic Sea

Pikeperch and perch perform seasonal migrations between the Curonian Lagoon and the coastal waters of the Baltic Sea. The Curonian Lagoon is a freshwater basin, while salinity in the coastal waters varies between of 4.9–6.8 psu. In the Curonian Lagoon water temperature is generally higher than in the coastal waters. Field studies of growth and condition characteristics of pikeperch and perch were carried out in these water bodies with the aim to estimate growth differences of the two fish species under different salinity and temperature conditions. Additionally, an experimental study of the impact of salinity on the growth of perch young-of-the-year (YOY) was performed to test the hypothesis that a brackish environment positively influences percids. Field observations revealed that body length, condition factor, fatness coefficient and fat content in muscles were significantly higher in individuals inhabiting the cooler, brackish waters of the Baltic Sea than in individuals inhabiting the Curonian Lagoon. A positive effect of low salinity on growth was also established in the experimental study. Hence, the study results suggest that under certain temperature conditions, brackish waters beneficially affect the growth of pikeperch and perch.     

Remote locality of the littoral Carex extensa (Cyperaceae) in Hungary — long distance dispersal from coastal to inland salt marshes

A remarkable population of Carex extensa (Cyperaceae) was found south of Lake Fert? (Neusiedler See) in Hungary in 2012. This species typically occurs in coastal salt marshes in Europe and was hitherto unknown from the Pannonian Basin. The locality is situated in a territory which has been embanked in 1911. Keeping also in mind that the vegetation developed here from reed beds to saline habitats, we conclude that C. extensa reached the salt marshes of the area through long distance dispersal by water birds from the European coast in the last century. Nevertheless, several other maritime littoral species grow in the region, and the possibility that there is a hidden population of C. extensa at another site(s) nearby cannot be excluded. Lake Fert? is often described as the “westernmost steppe lake” and supports one of the richest European inland littoral floras. Although it appears likely that C. extensa has only recently colonised the site, we consider this discovery to be of significant biogeographical importance.     

Carbon isotopes in otolith amino acids identify residency of juvenile snapper (Family: Lutjanidae) in coastal nurseries

This study explored the potential for otolith geochemistry in snapper (Family: Lutjanidae) to identify residency in juvenile nursery habitats with distinctive carbon isotope values. Conventional bulk otolith and muscle stable isotope analyses (SIA) and essential amino acid (AA) SIA were conducted on snapper collected from seagrass beds, mangroves, and coral reefs in the Red Sea, Caribbean Sea, and Pacific coast of Panama. While bulk stable isotope values in otoliths showed regional differences, they failed to distinguish nursery residence on local scales. Essential AA δ¹³C values in otoliths, on the other hand, varied as a function of habitat type and provided a better tracer of residence in different juvenile nursery habitats than conventional bulk otolith SIA alone. A strong linear relationship was found between paired otolith and muscle essential AA δ¹³C values regardless of species, geographic region, or habitat type, indicating that otolith AAs recorded the same dietary information as muscle AAs. Juvenile snapper in the Red Sea sheltered in mangroves but fed in seagrass beds, while snapper from the Caribbean Sea and Pacific coast of Panama showed greater reliance on mangrove-derived carbon. Furthermore, compound-specific SIA revealed that microbially recycled detrital carbon, not water-column-based new phytoplankton carbon, was the primary carbon source supporting snapper production on coastal reefs of the Red Sea. This study presented robust tracers of juvenile nursery residence that will be crucial for reconstructing ontogenetic migration patterns of fishes among coastal wetlands and coral reefs. This information is key to determining the importance of nursery habitats to coral reef fish populations and will provide valuable scientific support for the design of networked marine-protected areas.     

Reedbed structure and habitat preference of reed passerines during the post-breeding period

Post-breeding habitats of nine passerine species were studied in the permanently flooded reed beds of ponds near the village Pusté úlany (SW Slovakia) in 2002. Structural features of reed beds and the abundance of all birds studied were sampled at two sites and eight study plots. The reed bed structure and abundance of four passerines differed considerably between the individual study plots. The variations in vegetation structure were also caused by winter reed burning in the SE part of study area. Stems in burnt reed beds were smaller and thinner than unburnt. Abundance of the reed warbler and to a lesser extent sedge warbler was higher in tall reed beds, while the bearded tit preferred thick reed stems. Position of reed beds along the shore — open water gradient was an important factor in terms of abundance at the study plots in the case of the reed bunting. This species showed a strong preference for the reed bed edge with open water. Horizontal distribution of other species seems to be random, however, the birds with the exception of Savi’s warbler preferred the upper stratum of reed bed in vertical distribution. Our field data corroborate with those found in experimental studies concerning with ecomorphological characteristics of birds. Some inconsistencies, however, could also be caused by other factors (e.g. foraging preferences). Comparison of breeding and post-breeding habitat requirements of the studied bird species confirmed a more or less random distribution of the majority of species in the post-breeding period. Some variation was found in the cases of only the reed warbler and bearded tit.     

Diet,stable isotopes and morphology of Eurasian perch (Perca fluviatilis) in littoral and pelagic habitats in the northern Baltic Proper

We present morphology, stable isotope signals and stomach contents of Eurasian perch (Perca fluviatilis) from littoral and pelagic habitats in a brackish water embayment in the northern Baltic Proper. Studies conducted in freshwater habitats repeatedly state that littoral perch have deeper bodies than the pelagic ones. In this study we observed the opposite; the perch from the pelagic study site had deeper bodies than the littoral ones, indicating that more factors than habitat structure affect the perch morphology. A possible explanation to this discrepancy is the diet choice; the pelagic perch in this study were more benthivorous than freshwater pelagic perch. Our results on stable isotope signals combined with the stomach contents also shed new light on the dietary preferences of perch. Perch is known to be a generalist predatory fish, but our results indicate that perch have individual diet preferences. Based on our results, it seems that at some point in their lives the perch in brackish water choose between the littoral and pelagic habitats and also specialise in a certain diet. This study shows that the perch morphology and diet in the Baltic Sea coast differ among habitats, but the patterns are not similar to those observed in freshwater studies.     

Food competition and niche separation between fish and the Red-necked Grebe Podiceps grisegena (Boddaert, 1783)

During the breeding season, the aquatic bird Red-necked Grebe Podiceps grisegena feeds on small aquatic animals, which are also an important food source for many fish species. Because grebes have to provide substantial amounts of invertebrates to their brood, competitive interactions with fish may be expected during the breeding season of grebes. Based on this hypothesis, the distribution of Red-necked Grebes, the abundances of macroinvertebrates, fish, and vegetation, as well as the water depth were determined in a shallow lake during two consecutive breeding seasons. The Red-necked Grebes only utilised the reed bed as breeding site, whereas the adjacent Lagoon, although potentially suitable as breeding site, was not used. Macroinvertebrate biomass was 5–10 times higher in the reed beds than in the Lagoon. The abundance of fish was low in the reed bed but considerably higher in the Lagoon. The results indicate habitat separation between breeding Red-necked Grebe and fish in Lake Tåkern, and that the separation is related to distribution of food resources birds and fish have in common.     

Inter-annual variation of rotifer biomass in two coastal lagoons of the Southern Baltic differing by degree of trophy

We present long-term data on rotifer biomass and its inter-annual variation in the Darss-Zingst estuary and the Vistula Lagoon, South Baltic Sea coast. The two water bodies showed a similar pattern with respect to the dominance structure of the rotifer community, though in the more eutrophic Darss-Zingst estuary there was always a more pronounced summer peak. It was also concluded that there was no simple coincidence between variation patterns of rotifer biomass and such environmental factors as water temperature and salinity, and that a more complex explanation for these variations should be sought.     

Effects of ship‐induced waves on littoral benthic invertebrates

1. Ship‐induced waves can affect the physical characteristics of lake and river shorelines, and laboratory studies have shown effects on littoral invertebrates. Here, we explored whether these effects could be observed under field conditions along a natural lake shore affected by wave sequences (trains) produced by boats. 2. Individuals of five invertebrate species (Bithynia tentaculata, Calopteryx splendens, Dikerogammarus villosus, Gammarus roeselii, Laccophilus hyalinus) were exposed to waves with increasing shear stress in five habitats differing in structural complexity. 3. Detachment of invertebrates increased with increasing shear stress and was best modelled using sigmoid response curves. Habitat structural complexity mitigated the effects of shear stress, and detachment rate was influenced more by habitat type than by species. A threshold (90% of the individual invertebrates unaffected) stress level of 0.64 N m^?2 was found for a structurally complex reed habitat, compared to 0.37 N m^?2 for a simple sand habitat. 4. Shear stress associated with wave trains created by recreational boating at a distance of 35 m from the shore and at a speed of 11 km h^?1 resulted in 45% detachment of littoral invertebrates. Decreasing the boat‐to‐shore distance to 20 m increased wave shear stress by 30% and invertebrate detachments up to 75%. 5. Disturbance of littoral habitats and invertebrate assemblages are widespread in inland waters used for recreational and/or commercial navigation. Our findings show that the integrity of littoral zones of navigable surface waters could be much improved by implementing management measures such as physically protecting complex habitats with dense reed belts and tree roots, and reducing boat speeds and increasing their minimum shoreline distance.     

Fish assemblages across a complex,tropical freshwater/marine ecotone

Synopsis Riverine fish assemblages in the temperate zone generally show strong longitudinal patterns of faunal turnover and increases in species richness with increasing stream order. We examined the composition and structure of tropical fish assemblages across a complex freshwater/marine ecotone in Tortuguero National Park on the Caribbean coast of Central America. Species turnover was high between four characteristic habitats that largely corresponded with a longitudinal gradient of stream order over distances of less than 30 km. Suites of common fish species characterized each habitat: creeks, rivers, lagoons, and the sea. In addition to the habitat endemics, several species spanned two habitat types, but only three species were collected in more than two habitats. Multivariate gradient analysis of fish assemblages reflected a gradient of habitats that to some extent corresponded to fluvial distances. Due to the unusual configuration of coastal lagoons lying parallel to the coast, the ordination gradient showed little correlation with linear distance to the coast. Environmental variables related to habitat size and salinity showed greatest correspondence with the fish assemblage ordination gradient. Invertebrate-feeding fishes were the predominant trophic group in 15 of 16 fish assemblages, and inland creek sites contained a greater proportion of herbivores and omnivores than other sites. The relative fraction of herbivorous and detritivorous fishes showed a monotonic decline along the longitudinal habitat gradient from inland to coast. Patterns of species composition and richness at Tortuguero Park appeared to agree well with earlier models of factors influencing temperate zone stream fishes. Headwaters have low aquatic primary productivity and contain small colonizing fish species subject to large fluctuations in local densities and intermittent competition. Lagoons contain both large and small species, the latter being restricted largely to shallow edge habitats by predation. Lagoons exhibit more lentic environmental conditions, experience relatively fewer periodic disturbances than headwaters, and their assemblages are inferred to be under relatively greater influence of biotic factors. Fish assemblages of rivers and caños (swampy side channels and braids) appear to be under less abiotic control than headwaters and influenced less by biotic factors than lagoons.     

On the Potential Contribution of Microfungi to the Decomposition of Reed Leaf Detritus in a Coastal Lagoon: A Laboratory and Field Experiment

The potential contribution of microfungi to reed decomposition in a coastal habitat (Le Cesine Lagoon, Italy) was investigated under laboratory and field conditions. Leaf pack mass and surface loss, ergosterol content and O₂ uptake were used to construct carbon budgets and an empirical ergosterol‐to‐O₂ uptake relationship based on literature data was used to estimate the contribution of microfungi. Under laboratory conditions, reed carbon loss was entirely due to leaching and microbial respiration. In contrast, C losses observed in the field were accounted for by microbial respiration and macroinvertebrate shredding almost equally. Microfungi were estimated to account for 98% and 69% of microbially‐respired carbon under laboratory and field conditions, respectively. Our results provide a preliminary, quantitative assessment of microfungal contribution to reed decomposition in brackish habitats. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)     

Macroinvertebrate communities in Phragmites australis (Cav.) Trin. ex Steud. reed beds and open bank habitats in central victorian streams in Australia

Reed invasion is a common phenomenon of open streams with disturbed riparian vegetation in river catchments. Knowledge of the effects of such vegetation change on aquatic communities is fundamental to river management. Macroinvertebrate fauna in Phragmites australis (Cav.) Trin. ex Steud. and open bank habitats were examined in three rivers in central Victoria in order to understand the effect of such littoral habitat on macroinvertebrates. Data were analysed using Partially Nested Factorial ANOVA with season, river and habitats as main effects. Habitat structure had a significant effect (p<0.05) on macroinvertebrate species richness, however this was not seasonally consistent across the three rivers. There was a significant increase (p<0.05) in macroinvertebrate taxa richness in Phragmites habitats during winter and spring seasons. Total abundance of taxa showed no consistent significant differences in the two habitats. Results of Canonical Analysis of Principle Coordinates indicated significant differences (p<0.05) in macroinvertebrate assemblages between Phragmites and bare bank habitats in all seasons. Habitat selection by taxa could be related to the microphysical environment of the habitats. This study suggests that reed beds create important littoral habitat structures which support diverse macroinvertebrate assemblages.     

Remote sensing evidence for the episodic transport of phosphorus from the littoral zone of a thermally stratified lake

• 1 Esthwaite Water in Cumbria is a small, thermally stratified lake fringed with beds of reed in the shallow littoral. In this study, we used a combination of in situ measurements and airborne remote sensing to investigate some of the physical processes influencing the transport of water and nutrients from the littoral zone.
• 2 The analysis of water samples collected from the reed beds, the littoral zone and the open water showed that significantly higher concentrations of dissolved reactive phosphorus (DRP) were frequently recorded in the reed beds in early summer.
• 3 Experiments with surface and near‐surface free‐running drogues demonstrated that the movement of water from the littoral zone was strongly influenced by the development of a secondary thermocline. When there was no secondary stratification, the surface currents generated by light winds seldom exceeded 2 cm s^‐1. When a secondary thermocline was present, surface current speeds of 5–10 cm s^‐1 were recorded even when the wind speed was less than 200 cm s^‐1.
• 4 A series of thermal surveys using a Daedalus Airborne Thematic Mapper (ATM) demonstrated that plumes of warm water frequently developed in the littoral zone when the weather was calm. Some of these plumes covered several hundred square metres and persisted for several hours. Others were only a few metres in width and acted as episodic ‘pumps’ that appeared and dispersed in less than an hour.

     

Ostracoda as bioindicators of anthropogenic impact in salt lakes,saltpans, and a lagoon: A case study from the Gulf of Saros coast (NE Aegean Sea), Turkey

The study area consists of wetlands and semi-wetlands (saltpan and salt lakes) that are separated from the Gulf of Saros in the northeastern Aegean Sea by a littoral cordon. Twenty-four samples were collected from the Enez Salt Lake; Dalyan, Enez Gala, Kuvalak, Isik, and Tasalti Lakes; Vakif and Erikli Saltpans; and Karagol Lagoon. The overall distribution of the sediment sizes reveals that they consist predominantly of mud and sand and a mixture of mud, sand, and gravel. The average proportion of gravel size particles is 8.36%. Feldspar and mica are abundant in sand and gravel-sized materials. Ecological conditions of these coastal areas were assessed on the basis of ostracod assemblages that can be considered as useful bioindicators and depend on water depth, temperature, and salinity. In this context, the effects of physical and chemical properties of the environment, geochemistry of the sediment, and anthropogenic impact on the meiofauna, in particular ostracoda, was here evaluated. Cyprideis torosa Jones, a cosmopolitan species of ostracoda, was found in salt lakes. Other species of ostracoda were shown to be affected by environmental conditions. The toxicity of some heavy metals in the saltpans, salt lakes, and hypersaline lagoons was evaluated. Lake Dalyan and Erikli Saltpan display high toxicity for Cu and Cd levels. The distribution of hydrocarbon pollution of terrestrial origin was determined.     

Insertion sequences enrichment in extreme Red sea brine pool vent

Mobile genetic elements are major agents of genome diversification and evolution. Limited studies addressed their characteristics, including abundance, and role in extreme habitats. One of the rare natural habitats exposed to multiple-extreme conditions, including high temperature, salinity and concentration of heavy metals, are the Red Sea brine pools. We assessed the abundance and distribution of different mobile genetic elements in four Red Sea brine pools including the world’s largest known multiple-extreme deep-sea environment, the Red Sea Atlantis II Deep. We report a gradient in the abundance of mobile genetic elements, dramatically increasing in the harshest environment of the pool. Additionally, we identified a strong association between the abundance of insertion sequences and extreme conditions, being highest in the harshest and deepest layer of the Red Sea Atlantis II Deep. Our comparative analyses of mobile genetic elements in secluded, extreme and relatively non-extreme environments, suggest that insertion sequences predominantly contribute to polyextremophiles genome plasticity.

     

Use of a flooded salt marsh habitat by an endangered cyprinodontid fish (<Emphasis Type="Italic">Aphanius iberus</Emphasis>)

We report the first data on the use of occasionally inundated habitats in a Mediterranean coastal lagoon by the Spanish toothcarp (Aphanius iberus), a cyprinodontid fish in danger of extinction. During a flooding period, we sampled the fish population and the macroinvertebrate community in three contrasting habitats: mats of green algae, open water, and a habitat dominated by glasswort (Salicornia patula) that is occasionally inundated. The three habitats displayed strong variation in density and species composition of invertebrates in the water column and the benthos. In general, algal mats had higher invertebrate biomasses, but glasswort had higher diversity of organisms, in part of terrestrial origin. The density of toothcarp was very low in the open water. The habitat that is occasionally inundated (glasswort) significantly had the highest density of mature toothcarp, while immature fish were similarly abundant in the glasswort and algal mat habitats. Condition (weight–length relationship) and total food biomass in the gut contents of immature toothcarp was significantly higher in algal mats than in glasswort, whereas there were no such differences for mature fish. Therefore, the occasionally flooded habitat (glasswort) was positively selected by large mature toothcarp but seemed a disadvantageous habitat for immature individuals. Handling editor: L. Mauricio Bini.     

Fish Assemblages in Different Shallow Water Habitats of the Venice Lagoon

The small-sized fish assemblages of the Venice Lagoon were investigated and compared among five shallow subtidal habitats (seagrass beds, sparsely vegetated habitats, unvegetated sand bottoms, mudflats and saltmarsh creeks) in the Northern lagoon basin. Sampling was carried out seasonally (Spring, Summer and Autumn of 2002) in 4–7 stations for each habitat type, by means of a fine-mesh, small beach seine. Two-way analysis of variance was applied to assess the differences in species richness, fish diversity, density and standing stock amongst habitats, whereas fish assemblage composition was investigated by using multivariate analyses (MDS, ANOSIM, SIMPER). The analyses indicated that seagrass beds and saltmarsh creeks are relevant shallow habitats in structuring the small-sized fish assemblages of the Venice Lagoon, supporting specialized and recognizable fish assemblages. Those in seagrass beds, in particular, were characterized by higher species richness and standing stock with respect to all the others. The structuring role of these habitats was discussed in terms of both habitat complexity and degree of confinement. In contrast, sandy bottoms, mudflats and sparsely vegetated habitats were identified as “transition” habitats, with highly variable fish assemblages, influenced by the contribution of the adjacent habitats, and acting probably as both ‘buffer zones’ between the other habitats and migration routes for many fish species in the lagoon.     

Lake morphometry predicts the degree of habitat coupling by a mobile predator

Habitat coupling is an ecosystem process whereby semi-discontinuous habitats are connected through the movement of energy and nutrients by chemical, physical or biological processes. One oft-cited example is that of littoral–pelagic coupling in lakes. Theory has argued that such habitat coupling may be critical to food web dynamics, yet there have been few empirical studies that have quantified ecological factors that affect the degree of habitat coupling in ecosystems. Specifically, the degree to which habitat coupling occurs across important physical gradients has largely been ignored. To address this, we investigate the degree of littoral habitat coupling (i.e. the degree to which a top predator lake trout, Salvelinus namaycush, derives energy from the littoral zone) along a gradient of lake shape, where lake shape modifies the relative quantity of coupled epilimnetic benthic and pelagic habitats within each lake. Herein we demonstrate that littoral habitat coupling is intensified in simple circular lakes compared to their reticulate counterparts in seven Canadian Shield lakes. Although the more reticulate lakes had larger areas of epilimnetic benthic habitat, littoral food sources comprised 11% compared to 24% of lake trout diet in reticulate and circular lakes, respectively. This heightened interaction in circular lakes also appears to translate into increased omnivory in more circular lakes compared to reticulate lakes such that lake trout of circular lakes have a significantly lower trophic position than lake trout of reticulate lakes (F_1,5=6.71 p=0.05). These results suggest that it is the accessibility of littoral production via thermal refugia, and not the amount of littoral production, that determines the degree to which lake trout couple littoral and pelagic habitats in lakes.     

Differences in Salinity Tolerance and Gene Expression Between Two Populations of Atlantic Cod (Gadus morhua) in Response to Salinity Stress

Populations of marine fish, even from contrasting habitats, generally show low genetic differentiation at neutral genetic markers. Nevertheless, there is increasing evidence for differences in gene expression among populations that may be ascribed to adaptive divergence. Studying variation in salinity tolerance and gene expression among Atlantic cod (Gadus morhua) from two populations distributed across a steep salinity gradient, we observed high mortality (45% North Sea cod and 80% Baltic Sea cod) in a reciprocal common garden setup. Quantitative RT-PCR assays for expression of hsp70 and Na/K-ATPase α genes demonstrated significant differences in gene regulation within and between populations and treatment groups despite low sample sizes. Most interesting are the significant differences observed in expression of the Na/K-ATPase α gene in gill tissue between North Sea and Baltic cod. The findings strongly suggest that Atlantic cod are adapted to local saline conditions, despite relatively low levels of neutral genetic divergence between populations.     

Noding of Cyprideis torosa valves (Ostracoda) – a proxy for salinity? New data from field observations and a long‐term microcosm experiment

Cyprideis torosa (Jones , 1850) (Ostracoda, Crustacea) is one of the most common marginal marine ostracod species in the Northern hemisphere. We investigate the relationship between variable noding of its valves and salinity as well as Ca²⁺ concentration in the ambient water, analysing populations from an in vitro experiment and field data from the southern Baltic Sea coast. There is a clear negative linear correlation between the proportion of noded individuals from our microcosms and salinity. Deficiency of Ca²⁺ causes heavier noding in laboratory cultures. The same effect can be seen in the field, however, the increase of noded individuals with falling salinity appears to be stepped, not linear. This pattern probably reflects the ability of the animals to wait some time until better salinity conditions occur within the highly variable conditions of estuaries and lagoons. At the southern Baltic Sea coast, proportions of more than 20% noded valves within a C. torosa population indicates salinities of up to 2 psu, up to 10% noded valves indicate salinities between 2 and 7 psu, and the lack of noded valves salinities > 7 psu. Stable salinity conditions as in the studied microcosms cause a shift of these salinity limits to 5 and 14 psu approximately but in a linear relationship between salinity and proportion of noded individuals. Hence, athalassic populations from more stable water bodies should be used for continuous and more detailed salinity trend reconstructions. Deficiency of Ca²⁺ (approximately < 120 mg/l) effects up to about 20% more noded individuals than in water with same salinity but with higher Ca²⁺ concentrations. The reproduction rates within the microcosms indicate a salinity optimum of C. torosa eggs of 8 psu whereas the optimum of the adults seems to be at least 14 psu (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)