On the taxonomic status of a Dugesia species from Buenos Aires (Platyhelminthes: Turbellaria: Tricladida)

The taxonomic and distributional statuses of the freshwater planarians of South America are imperfectly understood, and a comprehensive review is sorely needed. The species known from the vicinity of Buenos Aires, Argentina, was first described by Böhmig (1902) under the name Planaria dubia Borelli, 1895. Subsequent authors (Kenk, 1930, 1974; Cazzaniga & Curino, 1987) have cast doubt on this assignation. Some have assigned it to Dugesia anceps Kenk; Hyman (1959) was inclined to regard the Buenos Aires planarian as a geographical variant of Dugesia sanchezi Hyman. A comparison of D. sanchezi from the mountainous region of central Chile with specimens from Buenos Aires confirms that the Argentinian material is a distinct species.The differences between the two species are subtle. The Argentinian specimens are distinctive in their possession of infranucleate epithelia of the atria, penis, and bursal canal, as well as in their penial structure. In both species, the epithelia of the atria and bursal canal are glandular. Those of D. sanchezi are nucleate and their secretions are cyanophilous. In the Argentinian specimens, these same epithelia are infranucleate; but while the secretion of the bursal canal is indeed cyanophilous, that of the atrium is erythrophilous. The presence, in these same specimens, of cyanophilous glands opening into the penial vesicles, and of erythrophilous glands whose secretions contribute to a viscous plug in the ejaculatory duct, suggests a difference in the physiology of copulation.It is apparent that the Argentinian specimens are distinct from D. sanchezi Hyman, and probably they represent a new species, one to be described in detail elsewhere. In any case, Hyman's (1959) suggestion that D. sanchezi occurs near Buenos Aires is mistaken; so also is Cazzaniga's & Curino's (1987) identification of specimens from Buenos Aires as D. anceps.     

Coastal dune vegetation and pollen representation in south Buenos Aires Province, Argentina

Aim To establish the relationship between coastal dune vegetation and its pollen representation as an aid to interpret Holocene vegetation dynamics and environmental changes from pollen assemblages. Location The study area is situated on the temperate Atlantic coast of south Buenos Aires Province, Argentina (c. 39° S and 61°20′ W). Methods The vegetation of the active dune area adjacent to the beach was described on the basis of its floristic composition from 25 plots. Classification of the vegetation into distinct zones was carried out by cluster analysis. Surface samples were collected from each vegetation stand and analysed for their pollen composition. Pollen percentage data were analysed using principal components analysis in order to investigate the degree to which the different vegetation units can be distinguished by their pollen spectra. Pollen–vegetation relationships for selected taxa were explored using simple scatter plots and indices of association, under‐ and over‐representation. Indices of floristic diversity and palynological richness were used to assess the representation of the vegetation in the pollen spectra. Results and conclusions Five vegetation zones are defined on the basis of species composition and their quantitative variation: back shore, mobile dunes, slacks, semi‐fixed and fixed dunes. Pollen assemblages from back shore, mobile dunes and slacks are clearly differentiated from semi‐fixed and fixed dunes. Pollen assemblages differ considerably from the associated vegetation composition. Major discrepancies are caused by large differences in pollen and vegetation proportion of Hyalis argentea and Discaria americana. There is a considerable proportion of non‐local pollen in every spectrum. Pollen representation in the coastal dunes at Monte Hermoso is influenced by differences in pollen production, dispersal and preservation of individual taxa as well as by the spatial distribution of the vegetation, the topography of the dune system and the wind pattern. The pollen–vegetation relationship established in this study has important implication for understanding and interpreting fossil pollen records from coastal dune environments.     

Association of African Genetic Admixture with Resting Metabolic Rate and Obesity Among Women

Objective: To investigate the role of genetic admixture in explaining phenotypic variation in obesity‐related traits in a sample of African‐American women (n = 145) and to determine significant associations between obesity traits and admixture genetic markers. Research Methods and Procedures: Associations between genetic admixture and BMI, resting metabolic rate, fat mass, fat‐free mass, and bone mineral density were tested using linear regression considering the estimation of admixture by 1) a maximum‐likelihood approach (MLA) and 2) a Bayesian analysis. Results: Both the conservative MLA and the Bayesian approach support an association between African genetic admixture and BMI. Evidence for the associations of African genetic admixture with fat mass and fat‐free mass was supported by the Bayesian analysis; the MLA supported an association with bone mineral density. When the individual ancestry informative markers that were used to estimate admixture were tested for associations with BMI, significant associations were identified in chromosomes 1, 11, and 12. Discussion: These results provide evidence supporting the application of admixture mapping methods to the identification of genes that result in higher levels of obesity among African‐American women. Further research is needed to replicate and further explore these findings.     

Airborne pollen sampling in a wildlife reserve in the south of Buenos Aires province,Argentina

The objective of this project was to study the pollen spectrum of the Marahué Wildlife Reserve (Central Argentina), the quantity and quality of airborne pollen grains, and to evaluate the contribution of outside sources, to protect a natural habitat with xerophytic shrub vegetation (“monte”) (the term “monte” is used for the dominant xerophytic shrub vegetation in the “partido” of Villarino) approximately 90 km south of Bahía Blanca. Sampling was performed weekly throughout a whole calendar year (January–December, 2003) using a volumetric impact sampler. Observations of flowering in the field were performed at the same time as the aerial sampling. Herbarium specimens were collected to make pollen collections of the species found in the study area, for reference purposes. The most abundant pollen types were Poaceae (31.3% total annual pollen), Amaranthus/Chenopodiaceae (25.3%), Eucalyptus (5%), Brassicaceae (3.9%), and Plantago (3.9%). This study showed the dispersion dynamics of pollen grains from characteristic species of the “partido” (the province of Buenos Aires is divided into partidos which are roughly equivalent to counties) of Villarino, the exotic flora of the surrounding area, and the pollen types from outside the region.

Phytoplankton from the Southern Coastal Fringe of the Río de la Plata (Buenos Aires,Argentina)

Opinion This is a series of contributions, expressing explicit opinions with regard to contemporary topics in limnology. These texts will mostly be based on new books with a large impact, but can also refer to other currently debated topics. Documented reactions to these contributions should be sent to the editor-in-chief; they will be considered for publication in subsequent issues. This revised version was published online in July 2006 with corrections to the Cover Date.     

The population genetic effects of ancestry and admixture in a subdivided cattle breed

The genetic structure of the Dexter, a minority cattle breed with complex demographic history, was investigated using microsatellite markers and a range of statistical approaches designed to detect both admixture and genetic drift. Modern representatives of two putative ancestral populations, the Devon and Kerry, together with the different populations of the Dexter, which have experienced different demographic histories, were analysed. Breed units showed comparatively high levels of genetic variability ( H _E = 0.63–0.68); however, distinct genetic subgroups were detected within the Dexter, which could be attributed to known demographic events. Much lower diversity was identified in three small, isolated Dexter populations ( H _E = 0.52–0.55) and higher differentiation ( F _ST > 0.13) was found. For one of these populations, where strong selection has taken place, we also found evidence of a demographic bottleneck. Three methods for quantifying breed admixture were applied and substantial method-based variation in estimates for the genetic contribution of the two proposed ancestral populations for each subdivision of the Dexter was found. Results were consistent only in the case of a group consisting of selected Traditional Dexter animals, where the ancestor of the modern Kerry breed was also determined as the greater parental contributor to the Dexter. The inconsistency of estimation of admixture proportions between the methods highlights the potentially confounding role of genetic drift in shaping small population structure, and the consequences of accurately describing population histories from contemporary genetic data.     

Microevolution of African American dental morphology

The African American (AA) gene pool is primarily the result of gene flow between two biologically disparate groups: West Africans (WA) and Americans of western European descent (EA). This research utilizes characteristics of dental morphology to trace genetic relationships among WA, western Europeans (EU), AA, and European Americans. Dental morphological traits are useful for this purpose because they are heritable, do not remodel during life (although they can be lost to wear or pathology), and can be compared equally among samples from past and present populations. The results of this research provide new information about human microevolution through time and space in a biocultural setting. The mean measure of divergence is used to analyze dental morphological data from 1,265 individuals in 25 samples grouped by ancestry and time. Three hypotheses associated with admixture in AA are tested. When compared with known history, results from dental morphological data are equivocal in documenting admixture in AA. Dental morphological traits do appear to reflect admixture in AA. However, changes in trait frequencies do not closely correspond with important cultural events and trends such as the institutionalized racism of the Civil War and Jim Crow era. Results are mixed concerning whether AA with greater admixture were more likely to take part in the Great Migration to southern urban centers and to the North.     

A comparative analysis of the phytoplankton from six pampean lotic systems (Buenos Aires, Argentina)

Phytoplankton, as well as physical and chemical variables of six lotic systems of Buenos Aires province, tributaries of Río de la Plata river (Rodríguez, El Gato, El Pescado, Buñirrigo and J. Blanco streams, Samborombón river) were analysed and results were related to the degree of anthropic pollution. Surveys were carried out seasonally from May 1997 to June 1998. Sample sites were stablished at 2 or 3 stations depending on the length of the systems, from the headwaters to the mouths. Specific composition, species richness, dominance, diversity and eveness were assessed. Diatoms and clorophytes presented the highest number of species. Cyanobacteria were dominant in 60% of the samples and diatoms were subdominant in 42% of them. Samborombón river showed minimun and maximun phytoplankton densities among the six systems (50 cell.ml^–1 and 188475 cell.ml^–1, respectively). Indexes values showed a non-defined pattern, increasing or decreasing in the most polluted sites. The first axis of the PCA which included the physical and chemical variables is characterized by the nutrient concentrations and it is related to the pollution of these systems, separating samples of Rodríguez and El Gato streams, which presented high positive scores, from the rest of them. The second axis is represented by variables which determine the water conductivity and separates samples from the mouths of Samborombón river and Buñirrigo stream, which presented high positive scores, from the rest of them. The species PCA indicate that its distribution is related to the first two components of the chemical data set PCA. The first axis separates species found in high-conductivity sites, such as the mouths of Samborombón and Buñirrigo systems (i.e. Cocconeis placentula, Aulacoseira granulata, Melosira varians, Actinocyclus normanii, Dictyosphaerium ehrenbergianum) from those registered in low-conductivity sites, such as J. Blanco stream (i.e. Pseudanabaena catenata, Nitzschia gracilis, Mallomonas sp.). The second axis separates and mesosaprobic species from polluted sites -Rodríguez and El Gato streams- (i.e. Gomphonema parvulum, Navicula cryptocephala, Nitzschia palea, N. umbonata, Oscillatoria amphibia, Lyngbya limnetica, Euglena acus) from those found in oligosaprobic non-polluted sites – J. Blanco stream – (Nitzschia gracilis, Pseudanabaena catenata, Gomphonema clavatum).     

A new giant cypridid ostracod (Crustacea) from southern Buenos Aires Province,Argentina

Amphicypris argentinensis sp. nov., is described and illustrated from Laguna Caliba, an ephemeral fresh water lake from the southern coast of Buenos Aires Province, Argentina. Specimens were raised from dried sediment recovered from a bank of ostracod shells found at the edges of the lake. Deposition of progressively smaller ostracod valves stacked one into another (cup-in-cup structure) is documented. The geographical distribution and ecology of the genus is briefly discussed.     

Unequal contributions of male and female gene pools from parental populations in the African descendants of the city of Melo, Uruguay

In admixed populations, genetic contributions from males and females of specific parental populations can be of different proportions due to past directional mating during the process of genetic admixture. In this research paper, we provide evidence of such male- and female-specific differential admixture components of African, European, and American Indian origin in an admixed population from the city of Melo, in the northeastern region of Uruguay. From data on 11 autosomal markers from a sample of 41 individuals of mixed African descent, we estimated 47% African, 38% European, and 15% Amerindian contributions. In contrast, 6 mtDNA site-specific polymorphic markers showed that the mtDNA genome of these individuals was 52% African, 19% European, and 29% Amerindian, while from 3 Y-specific polymorphic sites, we estimated 30% African, 64% European, and 6% Amerindian contributions. We argue that this heterogeneity of admixture estimates results from disproportionate unions of European males with African and American Indian females from which this mixed African population was formed. Also, we argue that the asymmetry of the admixture estimates from the three sets of markers (autosomal, mtDNA, and Y-linked) is a result of the changes in the direction of mating during the history of the population. Implications of such evidence of directional mating are discussed, indicating the need of further demographic data for a quantitative assessment of the impact of directional mating on genetic structure of admixed populations.     

Recent admixture in an Indian population of African ancestry

Identification and study of genetic variation in recently admixed populations not only provides insight into historical population events but also is a powerful approach for mapping disease loci. We studied a population (OG-W-IP) that is of African-Indian origin and has resided in the western part of India for 500 years; members of this population are believed to be descendants of the Bantu-speaking population of Africa. We have carried out this study by using a set of 18,534 autosomal markers common between Indian, CEPH-HGDP, and HapMap populations. Principal-components analysis clearly revealed that the African-Indian population derives its ancestry from Bantu-speaking west-African as well as Indo-European-speaking north and northwest Indian population(s). STRUCTURE and ADMIXTURE analyses show that, overall, the OG-W-IPs derive 58.7% of their genomic ancestry from their African past and have very little inter-individual ancestry variation (8.4%). The extent of linkage disequilibrium also reveals that the admixture event has been recent. Functional annotation of genes encompassing the ancestry-informative markers that are closer in allele frequency to the Indian ancestral population revealed significant enrichment of biological processes, such as ion-channel activity, and cadherins. We briefly examine the implications of determining the genetic diversity of this population, which could provide opportunities for studies involving admixture mapping.     

Admixture analysis of a rural population of the state of Guerrero, Mexico

We studied 156 individuals of Native American descent from the city of Tlapa in the state of Guerrero in western Mexico. Most individuals' ethnicity was either Nahua, Mixtec, or Tlapanec, but self-identified Mestizos and individuals of mixed ethnicities were also included in the sample. We typed 24 autosomal, one Y-chromosome, and four mitochondrial ancestry-informative markers (AIMs) to estimate group and individual admixture proportions, and determine whether the admixture process involved directional gene flow between parental groups. When genetically defined (GD) Mestizos were excluded from the analysis, Native American ancestry represented approximately 98% of the population's gene pool, while European and West African ancestry represented approximately 1% each. Maternally inherited markers also showed an exceptionally high Native American contribution (98.5%), as did the paternally inherited marker, DYS199 (90.7%). We did not detect genetic structure in this population using these AIMs, which appears consistent with the homogeneity of the sample in terms of admixture proportions. The addition of GD Mestizos to the sample did not produce a considerable change in admixture estimates, but it had a major effect on population structure. These results show that the population of Tlapa in Guerrero, Mexico, has experienced little admixture with Europeans and/or West Africans. They also show that the impact of a small number of admixed individuals on an otherwise homogeneous population might have profound implications on subsequent ancestry/phenotype analysis and mapping strategies. We suggest that heterogeneity is a major characteristic of Mexican populations and, as a consequence, should not be disregarded when designing epidemiological studies of Mexican and Mexican American populations.     

Renal weights in the southern African black population

Renal weights of 430 adult black subjects coming to medicolegal autopsy at the Diepkloof State Mortuary, a large urban area southwest of Johannesburg, South Africa, were analyzed. The subjects were from 10 southern African black ethnic groups-Zulu, Sotho, Tswana, Xhosa, Shangaan, Swazi, Venda, Ndebele, Kalanga, and Malawi. The aims of the study were: 1) to ascertain the anatomical "norm" as it pertains to renal weights in this diverse population group; 2) to formulate standard reference tables that might be of use to the practicing pathologist in the southern African arena; 3) to provide a range of values that take into account the variables of age, sex, race, body weight, and body height; and 4) to provide a standard of comparison with anthropological and anatomical studies conducted on North American black, North American Caucasian, Indian subcontinent, Burmese, and Jamaican population groups. In each of the 430 subjects, age, sex, ethnic group, supine body length, body weight, individual left and right renal weights, and causes of death were noted. The latter were divided into 6 categories: 1) penetrating incised wounds; 2) multiple injuries; 3) gunshot wounds; 4) craniocerebral injuries; 5) various miscellaneous nonnatural causes of death; and 6) natural causes of death. The above variables were analyzed by computer and compared with respect to renal weights. No statistically significant differences were observed between the sexes or the age groups.(ABSTRACT TRUNCATED AT 250 WORDS)     

Fish assemblage of a Pampasic stream (Buenos Aires,Argentina): temporal variations and relationships with environmental variables

In the Argentine Pampa fertile soils were originally covered by grasslands, but at present are intensively cultivated. We assessed the specific composition of the fish assemblage of El Pescado stream and compared it with that recorded in 1991–1993, when land use in the watershed consisted in natural pastures. The persistence of the fish assemblage between the two studies was rather high: 0.76. Abundance, biomass and species richness were higher during drought periods. Connectivity with the huge Río de la Plata hydrographic system seems the most important contribution to the high and stable species richness of El Pescado stream.     

Gene frequencies in the cat population of Buenos Aires, Argentina, and the possible origin of this population.

The phenotypes of 295 stray cats seen in the capital area of Buenos Aires, Argentina, between March and December of 1989 were recorded. The corresponding mutant allele frequencies were as follows: O = 0.28, a = 0.83, Ta = 0.01; tb = 0.31; d = 0.45; I = 0.40; S = 0.28; W = 0.02. The allele frequencies calculated at the O locus were consistent with those expected for a randomly breeding population according to the formula for the Hardy-Weinberg equilibrium. The analysis of the genetic distances between Buenos Aires and several European cat populations revealed that the Spanish and, especially, those with a proven more ancestral genetic constitution, are the most closely related. When a similar analysis was carried out, including other Latin American cat populations, those of Spanish origin were found to show the highest degree of relatedness. These findings suggest that the Buenos Aires cat population was not genetically structured following the "two-stepping-stone" model and support the hypothesis that differential gene flows play a transcendental role in understanding the genetic composition of domestic cat populations.     

Six new polymorphic microsatellite loci isolated and characterized from the African savannah elephant genome

The African savannah elephant (Loxodonta africana) is a ‘keystone’ species that plays a vital role in regulating the dynamics of both plant and animal communities and yet it is endangered and its numbers have been reduced to approximately 500 000 across their entire continental range. Molecular genetic markers are important tools for providing genetic information useful in formulating effective management and conservation strategies for the surviving elephant populations. We describe the isolation and characterization of six new polymorphic microsatellite markers in the African savannah elephant and demonstrate that these loci can be PCR (polymerase chain reaction)‐multiplexed, a desirable attribute that saves costs in large‐scale microsatellite screening.