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1.
Pollination ecology of four epiphytic orchids of New Zealand 总被引:4,自引:0,他引:4
BACKGROUND AND AIMS: In New Zealand epiphytic orchids are represented by four genera and eight species. The genera Earina (three species) and Winika (one species) are the most conspicuous and widespread. These are likely to be some of the southernmost distributed genera of epiphytic orchids in the world. METHODS: To identify the pollination strategies that have evolved in these orchids, hand-pollination treatments were done and floral visitors were observed in several wild populations at two areas of southern North Island (approx. 40 degrees S). Pollen:ovule ratio and osmophores were also studied and the total carbohydrate content of the nectar produced by each species was measured. KEY RESULTS: Earina autumnalis and Earina mucronata are self-compatible, whereas Earina aestivalis and Winika cunninghamii appear to be partially self-incompatible. All four orchids are incapable of autonomous selfing and therefore completely dependent on pollinators to set fruits. Floral visitors observed in the genus Earina belong to Diptera, Coleoptera and Hymenoptera and to Diptera and Hymenoptera in W. cunninghamii. CONCLUSIONS: Contrary to many epiphytic orchids in the tropics, the orchid-pollinator relationship in these orchids is unspecialized and flowers are visited by a wide range of insects. Putative pollinators are flies of the families Bibionidae, Calliphoridae, Syrphidae and Tachinidae. All four orchids display anthecological adaptations to a myophilous pollination system such as simple flowers, well-exposed reproductive structures, easily accessed nectar and high pollen : ovule ratios. 相似文献
2.
The firefly luciferase gene as a non-invasive reporter for Dendrobium transformation 总被引:9,自引:0,他引:9
Tet-Fatt Chia Yang-Sun Chan Nam-Hai Chua 《The Plant journal : for cell and molecular biology》1994,6(3):441-446
Here a screening method is described for transformed tissues and transgenic plants of Dendrobium (Orchidaceae) using the firefly luciferase gene ( luc ) as a combined marker/reporter gene. Protocorm-likebodies (PLB) were bombarded with tungsten particles (1.3 µm) coated with plasmids carrying a 35S-luc chimeric gene. Three weeks after bombardment 1 mM luciferin was added to the tissues and transformed cells were identified by virtue of their bioluminescence as monitored by low-light video microscopy in combination with a real-time photon imaging technique. Transformed tissues were excised, allowed to proliferate, and then subjected to a second round of screening. After three rounds of growth and screening, transformed Dendrobium tissues expressing luciferase were used to generate transgenic plants. Southern blot analysis of several transgenic lines confirmed the integration of the luciferase gene into the orchid genome. It is thought that this procedure can be used for transformation of not only orchids but other species as well. 相似文献
3.
兰科植物是开花植物中最大的家族之一,分子标记技术应用于兰科植物的分类鉴定和品种鉴别,为兰花的分类提供了分子水平的证据,也为兰花保护策略和措施的制定提供了理论基础。兰科植物表现有高度特异的形态、结构和生理特性,是研究花着色机理和子房发育的理想对象。兰花离体培养开花系统的建立,可以用来探明兰花从营养生长向生殖生长的转变机制,是研究花的分化和发育的理想材料。兰花具有特异的查尔酮合成酶(CHS)基因和二氢叶酸还原酶(DFR)基因等控制花色素的合成,DOH1基因控制石斛兰花芽的形成和提早开花,PHAL.039基因和ACC合成酶基因在蝴蝶兰授粉后的子房发育中起着重要的调控作用,这些特异基因的分离和克隆为兰花花的分化、发育及着色机制提供了分子基础。蝴蝶兰属、大花蕙兰(Cymbidium hybridium)、石斛兰属、文心兰属、五唇兰属和万代兰属等兰科植物都有转基因的研究报道,主要以原球茎为材料采用基因枪或农杆菌法转化,部分研究获得了转化植株。 相似文献
4.
重要观赏兰科植物的分子生物学研究进展 总被引:15,自引:0,他引:15
兰科植物是开花植物中最大的家族之一,分子标记技术应用于兰科植物的分类鉴定和品种鉴别,为兰花的分类提供了分子水平的证据,也为兰花保护策略和措施的制定提供了理论基础.兰科植物表现有高度特异的形态、结构和生理特性,是研究花着色机理和子房发育的理想对象.兰花离体培养开花系统的建立,可以用来探明兰花从营养生长向生殖生长的转变机制,是研究花的分化和发育的理想材料.兰花具有特异的查尔酮合成酶(CHS)基因和二氢叶酸还原酶(DFR)基因等控制花色素的合成,DOHI基因控制石斛兰花芽的形成和提早开花,PHAL039基因和ACC合成酶基因在蝴蝶兰授粉后的子房发育中起着重要的调控作用,这些特异基因的分离和克隆为兰花花的分化、发育及着色机制提供了分子基础.蝴蝶兰属、大花蕙兰(Cymbidium hybrdium)、石斛兰属、文心兰属、五唇兰属和万代兰属等兰科植物都有转基因的研究报道,主要以原球茎为材料采用基因枪或农杆菌法转化,部分研究获得了转化植株. 相似文献
5.
Hui Wang Huiyong Fang Yunqiang Wang Lisheng Duan Shunxing Guo 《World journal of microbiology & biotechnology》2011,27(9):2051-2059
The new in situ seed baiting method using seed packets to assess germination of orchid species within soil provides a means
of locating, collecting, and identifying specific fungi that are involved in the lifecycle of orchids in the wild and investigating
their relationship with the orchids under natural field conditions. Two isolates (SHH44, SHH53) originating from the seedlings
found in the seeds packets in situ were demonstrated to support seed germination and seedling development (in vitro) of two
endangered Chinese endemic herbs, Dendrobium officinale and Dendrobium nobile (Orchidaceae). Advanced protocorm development (Stage 3 and greater) in this study of these species of Dendrobium only occurred under the 12/12 h L/D photo-period indicated that illumination may play an important role in seedling recruitment
of terrestrial orchid species in their natural environment. The information provided in this study may prove invaluable in
the conservation of D. officinale and D. nobile and congener species in China, especially given their rare status. 相似文献
6.
报道江西兰科植物分布新记录2种——北插天天麻Gastrodia peichatieniana S. S. Ying和单葶草石斛Dendrobium porphyrochilum Lindley。凭证标本保存于九连山自然保护区植物标本室。 相似文献
7.
Xylem of the orchids studied provided unusually favorable material to demonstrate how conductive tissue evolves in monocotyledons. In the end walls of tracheary elements of many Orchidaceae, remnants of pit membranes were observed with scanning electron microscopy and minimally destructive methods. The full range from tracheids to vessel elements, featuring many intermediate stages, was illustrated with SEM in hand sections of fixed roots, stems, and inflorescence axes of 13 species from four subfamilies. Pit membranes in end walls of tracheary elements are porose to reticulate in roots of all species, but nonporose in stems of Cypripedioideae and Vanilloideae and porose to reticulate in stems of Orchidoideae and Epidendroideae. The distribution pattern of pit membranes and pit membrane remnants in end walls of tracheary elements of orchids parallels the findings of others. The position of Cypripedioideae and Vanilloideae as outgroups to Orchidoideae and Epidendroideae, claimed by earlier authors, is supported by clades based on molecular studies and by our studies. Little hydrolysis of pit membranes in tracheary element end walls was observed in pseudobulbs or inflorescence axes of epidendroids. The pervasiveness of network-like pit membranes of various extents and patterns in end walls of tracheary elements in Orchidaceae calls into question the traditional definitions of tracheids and vessel elements, not merely in orchids, but in angiosperms at large. These two concepts, based on light microscope studies, are blurred in light of ultrastructural studies. More importantly, the intermediate expressions of pit membranes in tracheary element end walls of Orchidaceae and some other families of angiosperms are important as indicators of steps in evolution of conduction with respect to organs (more rapid flow in roots than in succulent storage structures) and habitat (less obstruction to flow correlated with a shift from terrestrial to epiphytic). 相似文献
8.
A leading hypothesis for the immense diversity of the Orchidaceae is that skewed mating success and small, disjunct populations lead to strong genetic drift and switches between adaptive peaks. This mechanism is only possible under conditions of low gene flow that lead to high genetic differentiation among populations. We tested whether orchids typically exhibit high levels of population genetic differentiation by conducting a meta‐analysis to compare mean levels of population genetic differentiation (FST) between orchids and other diverse families and between rare and common orchids. Compared with other families, the Orchidaceae is typically characterized by relatively low genetic differentiation among populations (mean FST = 0.146) at allozyme loci. Rare terrestrial orchids showed higher population genetic differentiation than common orchids, although this value was still lower than the mean for most plant families. All lines of evidence suggest that orchids are typically characterized by low levels of population genetic differentiation, even in species with naturally disjunct populations. As such, we found no strong evidence that genetic drift in isolated populations has played a major role in the diversification of the Orchidaceae. Further research into the diversification of the family needs to unravel the relative roles of biotic and environmental selective pressures in the speciation of orchids. 相似文献
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N. H. WILLIAMS F.L.S. 《Botanical journal of the Linnean Society. Linnean Society of London》1976,72(4):299-309
Developmental studies of stomata in the tribes Cymbidieae, Maxillarieae and Gongoreae (Orchidaceae) demonstrate the presence of definite subsidiary cells in these groups of the Orchidaceae, contrary to recent reports that the Orchidaceae lack subsidiary cells. Fifteen species in these groups were studied developmentally and an additional 36 species were studied from mature leaves. In the Cymbidieae the subsidiary cells may be the lateral trapezoid cells derived from the lateral contact cells, or the subsidiary cells may be derivatives of the trapezoid cells. In the species of Maxillarieae and Gongoreae studied the subsidiary cells were derivatives of the trapezoid cells. The type of subsidiary cell development in these groups is essentially the same as has been found in other advanced groups of epidendroid orchids that have subsidiary cells. 相似文献
11.
Floral organ identity genes in the orchid Dendrobium crumenatum 总被引:1,自引:0,他引:1
Xu Y Teo LL Zhou J Kumar PP Yu H 《The Plant journal : for cell and molecular biology》2006,46(1):54-68
Orchids are members of Orchidaceae, one of the largest families in the flowering plants. Among the angiosperms, orchids are unique in their floral patterning, particularly in floral structures and organ identity. The ABCDE model was proposed as a general model to explain flower development in diverse plant groups, however the extent to which this model is applicable to orchids is still unknown. To investigate the regulatory mechanisms underlying orchid flower development, we isolated candidates for A, B, C, D and E function genes from Dendrobium crumenatum. These include AP2-, PI/GLO-, AP3/DEF-, AG- and SEP-like genes. The expression profiles of these genes exhibited different patterns from their Arabidopsis orthologs in floral patterning. Functional studies showed that DcOPI and DcOAG1 could replace the functions of PI and AG in Arabidopsis, respectively. By using chimeric repressor silencing technology, DcOAP3A was found to be another putative B function gene. Yeast two-hybrid analysis demonstrated that DcOAP3A/B and DcOPI could form heterodimers. These heterodimers could further interact with DcOSEP to form higher protein complexes, similar to their orthologs in eudicots. Our findings suggested that there is partial conservation in the B and C function genes between Arabidopsis and orchid. However, gene duplication might have led to the divergence in gene expression and regulation, possibly followed by functional divergence, resulting in the unique floral ontogeny in orchids. 相似文献
12.
Carolyn M. King 《Biological invasions》2016,18(6):1533-1550
The distribution of distinct genetic lineages of mice in New Zealand, combined with historical records of shipping routes, political decisions, market prices, trading patterns and immigration policy, suggest that two distinct lineages of Mus musculus travelled separate routes to reach opposite ends of New Zealand in early pre-colonial times (1792–1830). (1) Mus musculus castaneus could have colonised the southern South Island between 1792 and 1810, with sealers returning from the Canton fur market, but these voyages were illegal (=undocumented) because direct trading with China was prohibited until after 1813. Signs that the potential links between the South Island and Canton were seldom used after 1810 include: (a) the Canton sealskin market was already rapidly declining in profitability by the time sealers switched to New Zealand from Bass Strait in 1804; (b) the Otago colonies of fur seals (Arctocephalus forsteri) were exhausted after 1810; (c) M. m. castaneus is absent from the southern offshore islands repeatedly visited by Sydney-based sealers after 1810. (2) M. m. domesticus had multiple well-documented opportunities to colonise the Bay of Islands with traders from Australia after 1821, and both the Cook Strait area and the southern South Island with whalers after 1829. After 1840, multiple haplotypes of M. m. domesticus from different European sources accompanied the organised settlement of New Zealand by European colonists. 相似文献
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14.
新西兰位于南半球太平洋南部,介于南极洲和赤道之间,是目前为止南半球唯一一个将银杏作为产业来发展的国家。银杏在新西兰已有100多年的栽培历史,其南岛和北岛的各个城市和地区均栽植有银杏,但主要用作庭院、公园、街道绿化树种零星种植,直到21世纪初才开始被作为一种产业来发展。通过对新西兰的Auckland,Wellington,Hamilton,Hastings等主要城市的银杏种质资源进行广泛调查和定点观测,结果只发现有梅核类、佛指类、马铃类、园子类等四种类型。种核也较小,最大的为2.15g,最小的仅1.44g。本文主要报道了银杏在新西兰的栽培历史、生物学特性、种质资源及发展现状等,以期为中新两国开展在银杏方面的合作提供相关信息。 相似文献
15.
兰科植物欺骗性传粉 总被引:7,自引:0,他引:7
植物与传粉动物的互利关系在生态系统中非常普遍。然而,有许多植物不为传粉者提供任何报酬,而是利用各种欺骗方式诱骗昆虫拜访,从而实现传粉,即欺骗性传粉。兰科是被子植物大科之一,其高度特化的繁殖器官和适应于昆虫传粉的精巧结构令人称奇。进化论创始人达尔文描述了许多兰花与昆虫精巧的传粉系统,但他忽视了欺骗性传粉的存在。事实上,近1/3的兰科植物都依赖于欺骗性传粉。欺骗性传粉可能是导致兰科植物多样性的重要原因之一。兰花利用或操作昆虫觅食、交配、产卵和栖息等行为,演化出各种各样的欺骗性传粉机制,常见的类型包括泛化的食源性欺骗、Batesian拟态、性欺骗、产卵地拟态和栖息地拟态。花的颜色、形态和气味在欺骗性传粉的成功实现中起到了重要作用。欺骗性兰花与传粉昆虫之间的演化可能是不同步的,兰花追踪昆虫的行为信号而发生分化,然而欺骗性传粉可能对昆虫造成一定的伤害,从而对昆虫也施加选择压力。由于昆虫的学习行为,欺骗性的兰花一般具有低的昆虫拜访率和结实率,其繁殖成功率受各种因素的影响。欺骗性加剧了兰花对传粉昆虫的依赖,使其具有更高的灭绝风险,传粉生物学的研究能为兰科植物的有效保护提供指导。在欺骗性传粉系统中,有报酬的伴生植物、拟态模型和其他拟态信号提供者对传粉成功有重要影响。因此,研究欺骗性传粉兰花、传粉昆虫和相关的生物和生态因子的网状进化关系具有重要理论和实践意义。 相似文献
16.
与传统的连续检索表相比,基于计算机等数字终端的多通道交互式检索表具有很多优势,如易于携带、实时更新、界面直观、使用方便、用户广泛、产生新数据集且便于研究以及能够推动分类学知识的普及等。本文以兰科植物为例介绍了交互式检索表的特点、程序建立和应用。兰科植物是生物多样性保育的旗舰类群,具有很强的观赏和药用价值,但中国兰科植物种类繁多,分类困难。我们在自主研发交互式检索表软件MagIden的基础上,以《中国植物志》和Flora of China的描述为依据,制作了中国兰科植物交互式电子检索表,为分类学研究者和爱好者提供了一个新的、鉴定中国兰科植物的中英文双语工具;同时,交互式检索表生成的MVSP和Bayes形态特征数据集可用于聚类分析和分支分类学研究。交互式检索表在数据积累、更新和分析利用等方面具有独特的优势,是提高传统植物分类学研究与应用效率的重要工具。 相似文献
17.
Cameron KM 《Molecular phylogenetics and evolution》2004,31(3):1157-1180
DNA sequences of the plastid gene psaB were completed for 182 species of Orchidaceae (representing 150 different genera) and outgroup families in Asparagales. These data were analyzed using parsimony, and resulting trees were compared to a rbcL phylogeny of Orchidaceae for the same set of taxa after an additional 30 new rbcL sequences were added to a previously published matrix. The psaB tree topology is similar to the rbcL tree, although the psaB data contain less homoplasy and provide greater bootstrap support than rbcL alone. In combination, the two-gene tree recovers the five monophyletic subfamilial clades currently recognized in Orchidaceae, but fails to resolve the positions of Cypripedioideae and Vanilloideae. These new topologies help to clarify some of the anomalous results recovered when rbcL is analyzed alone. Both genes appear to be absent from the plastid genome of several achlorophyllous orchids, but are present in the form of presumably non-functional pseudogenes in Cyrtosia. This study is the first to document the utility of psaB sequences for phylogenetic studies of plants below the family level. 相似文献
18.
Cor J. Vink José G. B. Derraik Craig B. Phillips Phil J. Sirvid 《Biological invasions》2011,13(4):1003-1019
Populations of the Australian redback spider, Latrodectus hasseltii Thorell 1870, were first recorded in New Zealand in the early 1980s and in Osaka, Japan in 1995. Reliable records suggest that naturalised populations of L. hasseltii in New Zealand are present only in Central Otago and New Plymouth. In Central Otago, L. hasseltii feeds on endangered invertebrates, such as Prodontria modesta (Broun 1909). Latrodectus hasseltii is also a hazard to the New Zealand endemic L. katipo through interbreeding and competitive displacement. CLIMEXTM was used to model the potential global distribution of L. hasseltii based on current climate, and using ArcGIS® 9.2, areas of suitable climate in New Zealand were overlaid with favourable habitats to identify areas most suitable for L. hasseltii establishment. In addition, shelter that urban areas offer L. hasseltii were modelled in CLIMEX and incorporated into ArcGIS to produce maps indicating cities and built up areas where the species could establish. The presence of L. hasseltii in New Zealand and Japan, and its possible spread to other areas, is of human health significance, and the species may also impact on native biodiversity. 相似文献
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20.
Speciation in the Orchidaceae: confronting the challenges 总被引:2,自引:1,他引:1
Peakall R 《Molecular ecology》2007,16(14):2834-2837
The Orchidaceae is renowned for its large number of species (19,500) and its many diverse, even bizarre, specialized pollination systems. One unusual feature of orchids is the high frequency of food deception whereby animal pollination is achieved without providing nectar, pollen or other food rewards. Food-deceptive pollination is estimated to occur in approximately one-third of all orchids. Equally intriguing is pollination by sexual deception whereby pollination is achieved by the sexual attraction of male insects to the orchid flower. Sexual deception is found in several hundred species representing multiple lineages. Given their rich species diversity and extraordinary plant-animal interactions, orchids clearly offer exciting research opportunities in pollination biology, reproductive isolation and speciation, yet surprisingly they remain under-represented in scientific investigations both in these fields and more generally. In this special issue of Molecular Ecology, Moccia et al. provide an exemplar study that combine multiple lines of evidence to illuminate the mechanism of reproductive isolation between two closely related food-deceptive orchids. Their study demonstrates that many of the challenges that confront orchid researchers and impede progress in our understanding of speciation in the Orchidaceae can be overcome by the creative application and integration of both old and new tools in ecology and genetics. 相似文献