Automated tracking of muscle fascicle orientation in B-mode ultrasound images

B-mode ultrasound can be used to non-invasively image muscle fascicles during both static and dynamic contractions. Digitizing these muscle fascicles can be a timely and subjective process, and usually studies have used the images to determine the linear fascicle lengths. However, fascicle orientations can vary along each fascicle (curvature) and between fascicles. The purpose of this study was to develop and test two methods for automatically tracking fascicle orientation. Images were initially filtered using a multiscale vessel enhancement (a technique used to enhance tube-like structures), and then fascicle orientations quantified using either the Radon transform or wavelet analysis. Tests on synthetic images showed that these methods could identify fascicular orientation with errors of less than 0.06°. Manual digitization of muscle fascicles during a dynamic contraction resulted in a standard deviation of angle estimates of 1.41° across ten researchers. The Radon transform predicted fascicle orientations that were not significantly different from the manually digitized values, whilst the wavelet analysis resulted in angles that were 1.35° less, and reasons for these differences are discussed. The Radon transform can be used to identify the dominant fascicular orientation within an image, and thus used to estimate muscle fascicle lengths. The wavelet analysis additionally provides information on the local fascicle orientations and can be used to quantify fascicle curvatures and regional differences with fascicle orientation across an image.     

The effect of ultrasound probe orientation on muscle architecture measurement.

The purpose of this paper was to examine how muscle architecture parameter (MAP) measurements made using brightness-mode ultrasonography (BMU) differ based on probe orientation. The human tibialis anterior muscle was imaged from nine different probe orientations during concentric contractions at four joint angles to determine the effect of probe orientation on the measurement of muscle architecture parameters. Ankle dorsi-flexion torque, tibialis anterior electromyography (EMG), and measures of MAP including fascicle length (FL), pennation angle (PA) and muscle thickness (MT) were collected. Statistically significant differences were found between joint angles for measures of FL and PA and between probe orientations for measures of FL and MT. A comparison of actual MAP values to a geometric model used by researchers to determine an ideal probe orientation show that the actual values vary to a greater extent and do not follow the trend predicted by the model. The results suggest that ultrasound probe orientation affects measures of MAP but the effect either cannot be predicted from a geometric model and/or the error in the measurement technique does not allow a comparison.     

Reproducibility of gastrocnemius medialis muscle architecture during treadmill running

The purpose of this study was to assess the reproducibility of fascicle length (FL) and pennation angle (PA) of gastrocnemius medialis (GM) muscle during running in vivo. Twelve male recreational long distance runners (mean ± SD; age: 24 ± 3 years, mass: 76 ± 7 kg) ran on a treadmill at a speed of 3.0 m/s, wearing their own running shoes, for two different 10 min sessions that were at least 2 days apart. For each test day 10 acceptable trials were recorded. Ankle and knee joint angle data were recorded by a Vicon 624 system with three cameras operating at 120 Hz. B-mode ultrasonography was used to examine fascicle length and pennation angle of gastrocnemius medialis muscle. The ultrasound probe was firmly secured on the muscle belly using a lightweight foam fixation. The results indicated that fascicle length and pennation angle demonstrated high reproducibility values during treadmill running both for within and between test days. The root mean square scores between the repeated waveforms of pennation angle and fascicle length were small (∼2° and ∼3.5 mm, respectively). However, ∼14 trials for pennation angle and ∼9 trials for fascicle length may be required in order to record accurate data from muscle architecture parameters. In conclusion, ultrasound measurements may be highly reproducible during dynamic movements such as treadmill running, provided that a proper fixation is used in order to assure the constant location and orientation of the ultrasound probe throughout the movement.     

Computational methods for quantifying in vivo muscle fascicle curvature from ultrasound images

Muscle fascicles curve during contraction, and this has been seen using B-mode ultrasound. Curvature can vary along a fascicle, and amongst the fascicles within a muscle. The purpose of this study was to develop an automated method for quantifying curvature across the entirety of an imaged muscle, to test the accuracy of the method against synthetic images of known curvature and noise, and to test the sensitivity of the method to ultrasound probe placement. Both synthetic and ultrasound images were processed using multiscale vessel enhancement filtering to accentuate the muscle fascicles, wavelet-based methods were used to quantify fascicle orientations and curvature distribution grids were produced by quantifying local curvatures for each point within the image. Ultrasound images of ramped isometric contractions of the human medial gastrocnemius were acquired in a test–retest study.The methods enabled distinct curvatures to be determined in different regions of the muscle. The methods were sensitive to kernel sizes during image processing, noise within the image and the variability of probe placements during retesting. Across the physiological range of curvatures and noise, curvatures calculated from validation grids were quantified with a typical standard error of less than 0.026 m^?1, and this is about 1% of the maximum curvatures observed in fascicles of contracting muscle.     

Dynamic measurement of pennation angle of gastrocnemius muscles during contractions based on ultrasound imaging

ABSTRACT: BACKGROUND: Muscle fascicle pennation angle (PA) is an important parameter related to musculoskeletal functions, and ultrasound imaging has been widely used for measuring PA, but manually and frame by frame in most cases. We have earlier reported an automatic method to estimate aponeurosis orientation based on Gabor transform and Revoting Hough Transform (RVHT). METHODS: In this paper, we proposed a method to estimate the overall orientation of muscle fascicles in a region of interest, in order to complete computing the orientation of the other side of the pennation angle, but the side found by RVHT. The measurements for orientations of both fascicles and aponeurosis were conducted in each frame of ultrasound images, and then the dynamic change of pennation angle during muscle contraction was obtained automatically. The method for fascicle orientation estimation was evaluated using synthetic images with different noise levels and later on 500 ultrasound images of human gastrocnemius muscles during isometric plantarflexion. RESULTS: The muscle fascicle orientations were also estimated manually by two operators. From the results it's found that the proposed automatic method demonstrated a comparable performance to the manual method. CONCLUSIONS: With the proposed methods, ultrasound measurement for muscle pennation angles can be more widely used for functional assessment of muscles.     

Quantitative diffusion tensor MRI-based fiber tracking of human skeletal muscle.

Diffusion-tensor magnetic resonance imaging (DT-MRI) offers great potential for understanding structure-function relationships in human skeletal muscles. The purposes of this study were to demonstrate the feasibility of using in vivo human DT-MRI fiber tracking data for making pennation angle measurements and to test the hypothesis that heterogeneity in the orientation of the tibialis anterior (TA) muscle's aponeurosis would lead to heterogeneity in pennation angle. Eight healthy subjects (5 male) were studied. T(1)-weighted anatomical MRI and DT-MRI data were acquired of the TA muscle. Fibers were tracked from the TA's aponeurosis by following the principal eigenvector. The orientations of the aponeurosis and muscle fiber tracts in the laboratory frame of reference and the orientation of the fiber tracts with respect to the aponeurosis [i.e., the pennation angle (theta)] were determined. The muscle fiber orientations, when expressed relative to the laboratory frame of reference, did not change as functions of superior-to-inferior position. The sagittal and coronal orientations of the aponeurosis did not change in practically significant manners either, but the aponeurosis' axial orientation changed by approximately 40 degrees . As a result, the mean value for theta decreased from 16.3 (SD 6.9) to 11.4 degrees (SD 5.0) along the muscle's superior-to-inferior direction. The mean value of theta was greater in the deep than in the superficial compartment. We conclude that pennation angle measurements of human muscle made using DT-MRI muscle fiber tracking are feasible and reveal that in the foot-head direction, there is heterogeneity in the pennation properties of the human TA muscle.     

Ultrasound-based subject-specific parameters improve fascicle behaviour estimation in Hill-type muscle model

The estimation of muscle fascicle behaviour is decisive in a Hill-type model as they are related to muscle force by the force–length–velocity relationship and the tendon force–strain relationship. This study was aimed at investigating the influence of subject-specific tendon force–strain relationship and initial fascicle geometry (IFG) on the estimation of muscle forces and fascicle behaviour during isometric contractions. Ultrasonography was used to estimate the in vivo muscle fascicle behaviour and compare the muscle fascicle length and pennation angle estimated from the Hill-type model. The calibration–prediction process of the electromyography-driven model was performed using generic or subject-specific tendon definition with or without IFG as constraint. The combination of subject-specific tendon definition and IFG led to muscle fascicle behaviour closer to ultrasound data and significant lower forces of the ankle dorsiflexor and plantarflexor muscles compared to the other conditions. Thus, subject-specific ultrasound measurements improve the accuracy of Hill-type models on muscle fascicle behaviour.     

Determining skeletal muscle architecture with Laplacian simulations: a comparison with diffusion tensor imaging

Determination of skeletal muscle architecture is important for accurately modeling muscle behavior. Current methods for 3D muscle architecture determination can be costly and time-consuming, making them prohibitive for clinical or modeling applications. Computational approaches such as Laplacian flow simulations can estimate muscle fascicle orientation based on muscle shape and aponeurosis location. The accuracy of this approach is unknown, however, since it has not been validated against other standards for muscle architecture determination. In this study, muscle architectures from the Laplacian approach were compared to those determined from diffusion tensor imaging in eight adult medial gastrocnemius muscles. The datasets were subdivided into training and validation sets, and computational fluid dynamics software was used to conduct Laplacian simulations. In training sets, inputs of muscle geometry, aponeurosis location, and geometric flow guides resulted in good agreement between methods. Application of the method to validation sets showed no significant differences in pennation angle (mean difference \(0.5{^{\circ }})\) or fascicle length (mean difference 0.9 mm). Laplacian simulation was thus effective at predicting gastrocnemius muscle architectures in healthy volunteers using imaging-derived muscle shape and aponeurosis locations. This method may serve as a tool for determining muscle architecture in silico and as a complement to other approaches.     

A three-dimensional approach to pennation angle estimation for human skeletal muscle

Pennation angle (PA) is an important property of human skeletal muscle that plays a significant role in determining the force contribution of fascicles to skeletal movement. Two-dimensional (2D) ultrasonography is the most common approach to measure PA. However, in principle, it is challenging to infer knowledge of three-dimensional (3D) architecture from 2D assessment. Furthermore, architectural complexity and variation impose more difficulties on reliable and consistent quantification of PA. Thus, the purpose of our study is to provide accurate insight into the correspondence between 2D assessment and the underlying 3D architecture. To this end, a 3D method was developed to directly quantify PA based on 3D architectural data that were acquired from cadaveric specimens through dissection and digitization. Those data were then assessed two-dimensionally by simulating ultrasound imaging. To achieve consistency over intermuscular variation, our proposed 3D method is based on the geometric analysis of fascicle attachment. Comparative results show a wide range of differences (1.1–47.1%) between 2D and 3D measurements. That is, ultrasound can under- or over-estimate PA, depending on the architecture.     

Robust estimation of physiological cross-sectional area and geometric reconstruction for human skeletal muscle

Understanding muscle architecture is crucial to determining the mechanical function of muscle during body movements, because architectural parameters directly correspond to muscle performance. Accurate parameters are thus essential for reliable simulation. Human cadaveric muscle specimen data provides the anatomical detail needed for in-depth understanding of muscle and accurate parameter estimation. However, as muscle generally has non-uniform architecture, parameter estimation, specifically, physiological cross-sectional area (PCSA), is rarely straightforward. To deal effectively with this non-uniformity, we propose a geometric approach in which a polygon is sought to best approximate the cross-sectional area of each fascicle by accounting for its three-dimensional trajectory and arrangement in the muscle. Those polygons are then aggregated to determine PCSA and volume of muscle. Experiments are run using both synthetic data and muscle specimen data. From comparison of PCSA using synthetic data, we conclude that the proposed method enhances the robustness of PCSA estimation against variation in muscle architecture. Furthermore, we suggest reconstruction methods to extract 3D muscle geometry directly from fascicle data and estimated parameters using the level set method.     

Heterogeneous fascicle behavior within the biceps femoris long head at different muscle activation levels

Magnetic resonance and ultrasound imaging have shown hamstring strain injuries occur most often in the biceps femoris long head (BFLH), and particularly in the proximal vs. distal region of this muscle. Animal research and musculoskeletal modeling (MSK) have detected heterogeneous fascicle behavior within muscle regions, and within fascicles. Understanding architectural behavior differences during muscle contractions may help to discern possible mechanisms behind proximal BFLH injuries. The purpose of our study was to assess the magnitude of shortening of the proximal and distal fascicles of the BFLH under a range of muscle activation levels under isometric conditions using ultrasound imaging (US). Thirteen healthy adults performed targeted sustained isometric contractions while US were taken of the entire BFLH. Measurements of fascicle lengths in both muscle regions were compared at 20%, 30%, 50%, and 67% MVIC. The results showed that while both regions shortened significantly with activation, the proximal fascicles were significantly longer, regardless of activation level (~38%), and shortened significantly more than the distal fascicles overall (~40%), and cumulatively at higher activation levels (30% and above). No significant strain differences were found between the two regions. These data suggest heterogeneous fascicle behavior exists in an absolute sense; however, differences in behavior are eliminated when normalized (strain). Coupled with MSK literature, the absence of regional fascicle strain differences in this study may indicate strain heterogeneity is not detectable at the whole fascicle level. Further knowledge of this commonly strained muscle?s regional behavior during dynamic movements could provide evidence of proximal hamstring strain predisposition.     

Reliability and accuracy of an automated tracking algorithm to measure controlled passive and active muscle fascicle length changes from ultrasound

Manual tracking of muscle fascicle length changes from ultrasound images is a subjective and time-consuming process. The purpose of this study was to assess the repeatability and accuracy of an automated algorithm for tracking fascicle length changes in the medial gastrocnemius (MG) muscle during passive length changes and active contractions (isometric, concentric and eccentric) performed on a dynamometer. The freely available, automated tracking algorithm was based on the Lucas–Kanade optical flow algorithm with an affine optic flow extension, which accounts for image translation, dilation, rotation and shear between consecutive frames of an image sequence. Automated tracking was performed by three experienced assessors, and within- and between-examiner repeatability was computed using the coefficient of multiple determination (CMD). Fascicle tracking data were also compared with manual digitisation of the same image sequences, and the level of agreement between the two methods was calculated using the coefficient of multiple correlation (CMC). The CMDs across all test conditions ranged from 0.50 to 0.93 and were all above 0.98 when recomputed after the systematic error due to the estimate of the initial fascicle length on the first ultrasound frame was removed from the individual fascicle length waveforms. The automated and manual tracking approaches produced similar fascicle length waveforms, with an overall CMC of 0.88, which improved to 0.94 when the initial length offset was removed. Overall results indicate that the automated fascicle tracking algorithm was a repeatable, accurate and time-efficient method for estimating fascicle length changes of the MG muscle in controlled passive and active conditions.     

Age-related changes in human skeletal muscle microstructure and architecture assessed by diffusion-tensor magnetic resonance imaging and their association with muscle strength

Diffusion-tensor magnetic resonance imaging (DT-MRI) offers objective measures of muscle characteristics, providing insights into age-related changes. We used DT-MRI to probe skeletal muscle microstructure and architecture in a large healthy-aging cohort, with the aim of characterizing age-related differences and comparing these to muscle strength. We recruited 94 participants (43 female; median age = 56, range = 22–89 years) and measured microstructure parameters—fractional anisotropy (FA) and mean diffusivity (MD)—in 12 thigh muscles, and architecture parameters—pennation angle, fascicle length, fiber curvature, and physiological cross-sectional area (PCSA)—in the rectus femoris (RF) and biceps femoris longus (BFL). Knee extension and flexion torques were also measured for comparison to architecture measures. FA and MD were associated with age (β = 0.33, p = 0.001, R² = 0.10; and β = −0.36, p < 0.001, R² = 0.12), and FA was negatively associated with Type I fiber proportions from the literature (β = −0.70, p = 0.024, and R² = 0.43). Pennation angle, fiber curvature, fascicle length, and PCSA were associated with age in the RF (β = −0.22, 0.26, −0.23, and −0.31, respectively; p < 0.05), while in the BFL only curvature and fascicle length were associated with age (β = 0.36, and −0.40, respectively; p < 0.001). In the RF, pennation angle and PCSA were associated with strength (β = 0.29, and 0.46, respectively; p < 0.01); in the BFL, only PCSA was associated with strength (β = 0.43; p < 0.001). Our results show skeletal muscle architectural changes with aging and intermuscular differences in the microstructure. DT-MRI may prove useful for elucidating muscle changes in the early stages of sarcopenia and monitoring interventions aimed at preventing age-associated microstructural changes in muscle that lead to functional impairment.     

Inevitable joint angular rotation affects muscle architecture during isometric contraction

The purpose of this study was to quantify the influence of inevitable ankle joint motion during an isometric contraction on the measured change of the gastrocnemius medialis muscle (GM) architecture in vivo during the loading and the unloading phase. Sitting on a dynamometer subjects performed isometric maximal voluntary contractions as well as contractions induced by electrostimulation. Synchronous joint angular motion, plantarflexion moment, foot’s centre of pressure and real-time ultrasonography of muscle architecture changes of the GM were obtained. During the contraction the ankle joint position altered and significantly affected the change in muscle architecture. At maximal tendon force (1094 ± 323 N), the measured fascicle length overestimated the change in fascicle length due to the tendon force by 1.53 cm, while the measured pennation angle overestimated the change in pennation angle due to the tendon force by 5.5°. At the same tendon force the measured fascicle length and pennation angle were significantly different between loading and unloading conditions. After correcting the values for the change in ankle joint angle no differences between the loading and the unloading phase at the same tendon force were found. Concerning the estimation of GM fascicle length–force and pennation angle–force curves during the loading and unloading phase of an isometric contraction, these findings indicate that not accounting for ankle joint motion will produce unreliable results.     

Behavior of human muscle fascicles during shortening and lengthening contractions in vivo.

The aim of the present study was to investigate the behavior of human muscle fascicles during dynamic contractions. Eight subjects performed maximal isometric dorsiflexion contractions at six ankle joint angles and maximal isokinetic concentric and eccentric contractions at five angular velocities. Tibialis anterior muscle architecture was measured in vivo by use of B-mode ultrasonography. During maximal isometric contraction, fascicle length was shorter and pennation angle larger compared with values at rest (P < 0.01). During isokinetic concentric contractions from 0 to 4.36 rad/s, fascicle length measured at a constant ankle joint angle increased curvilinearly from 49.5 to 69.7 mm (41%; P < 0.01), whereas pennation angle decreased curvilinearly from 14.8 to 9.8 degrees (34%; P < 0.01). During eccentric muscle actions, fascicles contracted quasi-isometrically, independent of angular velocity. The behavior of muscle fascicles during shortening contractions was believed to reflect the degree of stretch applied to the series elastic component, which decreases with increasing contraction velocity. The quasi-isometric behavior of fascicles during eccentric muscle actions suggests that the series elastic component acts as a mechanical buffer during active lengthening.     

In vivo fascicle length measurements via B-mode ultrasound imaging with single vs dual transducer arrangements

Ultrasonography is a useful technique to study muscle contractions in vivo, however larger muscles like vastus lateralis may be difficult to visualise with smaller, commonly used transducers. Fascicle length is often estimated using linear trigonometry to extrapolate fascicle length to regions where the fascicle is not visible. However, this approach has not been compared to measurements made with a larger field of view for dynamic muscle contractions. Here we compared two different single-transducer extrapolation methods to measure VL muscle fascicle length to a direct measurement made using two synchronised, in-series transducers. The first method used pennation angle and muscle thickness to extrapolate fascicle length outside the image (extrapolate method). The second method determined fascicle length based on the extrapolated intercept between a fascicle and the aponeurosis (intercept method). Nine participants performed maximal effort, isometric, knee extension contractions on a dynamometer at 10° increments from 50 to 100° of knee flexion. Fascicle length and torque were simultaneously recorded for offline analysis. The dual transducer method showed similar patterns of fascicle length change (overall mean coefficient of multiple correlation was 0.76 and 0.71 compared to extrapolate and intercept methods respectively), but reached different absolute lengths during the contractions. This had the effect of producing force–length curves of the same shape, but each curve was shifted in terms of absolute length. We concluded that dual transducers are beneficial for studies that examine absolute fascicle lengths, whereas either of the single transducer methods may produce similar results for normalised length changes, and repeated measures experimental designs.     

Estimation of cat medial gastrocnemius fascicle lengths during dynamic contractions

In typical muscle models, it is often assumed that the contractile element (fascicle) length depends exclusively on the instantaneous muscle-tendon length and the instantaneous muscle force. In order to test whether the instantaneous fascicle length during dynamic contractions can be predicted from muscle-tendon length and force, fascicle lengths, muscle-tendon lengths, and muscle forces were directly measured in cat medial gastrocnemii during isometric and dynamic contractions. Two theoretical muscle models were developed: model A was based on force-time data obtained during the activation phase and model D on force-time data obtained during the deactivation phase of isometric contractions. To test the models, instantaneous fascicle lengths were predicted from muscle-tendon lengths and forces during dynamic contractions that simulated cat locomotion for speeds ranging from 0.4 to 1.6m/s. The theoretically predicted fascicle lengths were compared with the experimentally measured fascicle lengths. It was found that fascicle lengths were not uniquely associated with muscle-tendon lengths and forces; that is, for a given muscle-tendon length and force, fascicle lengths varied depending on the contractile history. Consequently, models A and D differed in fascicle length predictions; model D (maximum average error=8.5%) was considerably better than model A (maximum average error=22.3%). We conclude from this study that it is not possible to predict the exact fascicle lengths from muscle-tendon lengths and forces alone, however, adequate predictions seem possible based on such a model. The relationship between fascicle length and muscle force and muscle-tendon length is complex and highly non-linear, thus, it appears unlikely that accurate fascicle length predictions can be made without some reference contractions in which fascicle length, muscle-tendon length, and force are measured simultaneously.     

Effect of rhythmic tetanic skeletal muscle contractions on peak muscle perfusion.

The purpose of this investigation was to examine the effect of rhythmic tetanic skeletal muscle contractions on peak muscle perfusion by using spontaneously perfused canine gastrocnemii in situ. Simultaneous pulsatile blood pressures were measured by means of transducers placed in the popliteal artery and vein, and pulsatile flow was measured with a flow-through-type transit-time ultrasound probe placed in the venous return line. Two series of experiments were performed. In series 1, maximal vasodilation of the muscles' vascular beds was elicited by infusing a normal saline solution containing adenosine (29.3 mg/min) and sodium nitroprusside (180 microg/min) for 15 s and then simultaneously occluding both the popliteal artery and vein for 5 min. The release of occlusion initiated a maximal hyperemic response, during which time four tetanic contractions were induced with supramaximal voltage (6-8 V, 0.2-ms stimuli for 200-ms duration at 50 Hz, 1/s). In series 2, the muscles were stimulated for 3 min before the muscle contractions were stopped for a period of 3 s; stimulation was then resumed. The results of series 1 indicate that, although contractions lowered venous pressure, muscle blood flow was significantly reduced from 2,056 +/- 246 to 1,738 +/- 225 ml x kg(-1) x min(-1) when contractions were initiated and then increased significantly to 1,925 +/- 225 ml x kg(-1) x min(-1) during the first 5 s after contractions were stopped. In series 2, blood flow after 3 min of contractions averaged 1,454 +/- 149 ml x kg(-1) x min(-1). Stopping the contractions for 3 s caused blood flow to increase significantly to 1,874 +/- 172 ml x kg(-1) x min(-1); blood flow declined significantly to 1,458 +/- 139 ml x kg(-1) x min(-1) when contractions were resumed. We conclude that the mechanical action of rhythmic, synchronous, maximal isometric tetanic skeletal muscle contractions inhibits peak muscle perfusion during maximal and near-maximal vasodilation of the muscle's vascular bed. This argues against a primary role for the muscle pump in achieving peak skeletal muscle blood flow.     

Passive and dynamic muscle architecture during transverse loading for gastrocnemius medialis in man

External forces from our environment impose transverse loads on our muscles. Studies in rats have shown that transverse loads result in a decrease in the longitudinal muscle force. Changes in muscle architecture during contraction may contribute to the observed force decrease. The aim of this study was to quantify changes in pennation angle, fascicle dimensions, and muscle thickness during contraction under external transverse load.Electrical stimuli were elicited to evoke maximal force twitches in the right calf muscles of humans. Trials were conducted with transverse loads of 2, 4.5, and 10 kg. An ultrasound probe was placed on the medial gastrocnemius in line with the transverse load to quantify muscle characteristics during muscle twitches.Maximum twitch force decreased with increased transverse muscle loading. The 2, 4.5, and 10 kg of transverse load showed a 9, 13, and 16% decrease in longitudinal force, respectively. Within the field of view of the ultrasound images, and thus directly beneath the external load, loading of the muscle resulted in a decrease in the muscle thickness and pennation angle, with higher loads causing greater decreases. During twitches the muscle transiently increased in thickness and pennation angle, as did fascicle thickness. Higher transverse loads showed a reduced increase in muscle thickness. Smaller increases in pennation angle and fascicle thickness strain also occurred with higher transverse loads.This study shows that increased transverse loading caused a decrease in ankle moment, muscle thickness, and pennation angle, as well as transverse deformation of the fascicles.     

Three-dimensional analysis of the arrangement and length distribution of fascicles in the triceps muscle of Galea musteloides (Rodentia, Cavimorpha)

Non-uniformity of fascicle parameters (fascicle lengths and orientation) within one skeletal muscle is well known. These parameters have an effect on the physiological cross-sectional area and lengthening rate of the skeletal muscle. Using a binocular microscope with a table driver (q- and p-axes) and vertical drive (v-axis) as a tool for reconstruction of the spatial orientation of single muscle fascicles, we developed an approach for three-dimensional analysis of the arrangement and length distribution in the skeletal muscle of small mammals. Two subunits of the triceps brachii muscle of the Galea musteloides forelimb, triceps longum and triceps laterale, were quantified and compared. Our data show that in the triceps laterale the fascicles are significantly longer (10.23 mm, SD=1.19, n=41) than those in the triceps longum (6.58 mm, SD=2.88, n=39). In the triceps laterale, the fascicle orientation is more or less uniform, whereas, in the triceps longum, there are two areas with different orientation of fascicles: anterior and posterior ones. Different inner architecture of the subunits can be interpreted as an adaptation to the main locomotory function of the triceps muscle, namely production of propulsive force during limb transfer phase and keeping dynamic stability during stance phase. Comparison of our data on the fascicle length and geometry with our previous histochemical results on G. musteloides, shows that the anterior region of the triceps longum, which differs in the fascicle orientation, also contains a significantly larger percent of slow muscle fibres. It is hypothesised here that this small region is involved in keeping posture. Accepted: 16 May 2000