共查询到20条相似文献,搜索用时 93 毫秒
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A. Phongdara A. Merckelbach P. Keup G. Gellissen C. P. Hollenberg 《Applied microbiology and biotechnology》1998,50(1):77-84
A cloned cDNA, generated from mRNA isolates of phosphate-derepressed H. polymorpha cells, was identified to harbour an incomplete sequence of the coding region for a repressible acid phosphatase. The cDNA
fragment served as a probe to screen a plasmid library of H. polymorpha genomic DNA. A particular clone, p606, of a 1.9-kb insert contained a complete copy of the PHO1 gene. Sequencing revealed the presence of a 1329-nucleotide open reading frame encoding a protein of 442 amino acids with
a calculated M
r of 49400. The␣encoded protein has an N-terminal 17-amino-acid secretory leader sequence and seven potential N-glycosylation sites. The leader cleavage site was confirmed by N-terminal sequencing of the purified enzyme. The nucleotide
sequence is 48.9% homologous, the derived amino acid sequence 36% homologous to its Saccharomyces cerevisiae counterpart. The derived amino acid sequence harbours a consensus sequence RHGXRXP, previously identified as a sequence involved
in active-site formation of acid phosphatases. The PHO1 promoter and the secretion leader sequence present promising new tools for heterologous gene expression.
Received: 15 January 1998 / Received revision: 2 March 1998 / Accepted: 4 March 1998 相似文献
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Z. Li S. Rahman B. Kosar-Hashemi G. Mouille R. Appels M. K. Morell 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(8):1208-1216
A cDNA clone, and a corresponding genomic DNA clone, containing full-length sequences encoding wheat starch synthase I, were
isolated from a cDNA library of hexaploid wheat (Triticum aestivum) and a genomic DNA library of Triticum tauschii, respectively. The entire sequence of the starch synthase-I cDNA (wSSI-cDNA) is 2591 bp, and it encodes a polypeptide of 647
amino-acid residues that shows 81% and 61% identity to the amino-acid sequences of SSI-type starch synthases from rice and
potato, respectively. In addition, the putative N-terminal amino-acid sequence of the encoded protein is identical to that
determined for the N-terminal region of the 75-kDa starch synthase present in the starch granule of hexaploid wheat. Two prominent
starch synthase activities were demonstrated to be present in the soluble fraction of wheat endosperm by activity staining
of the non-denaturing PAGE gels. The most anodal band (wheat SSI) shows the highest staining intensity and results from the
activity of a 75-kDa protein. The wheat SSI mRNA is expressed in the endosperm during the early to mid stages of wheat grain
development but was not detected by Northern blotting in other tissues from the wheat plant. The gene encoding the wheat SSI
(SsI-D1) consists of 15 exons and 14 introns, similar to the structure of the rice starch synthase-I gene. While the exons of wheat
and rice are virtually identical in length, the wheat SsI-D1 gene has longer sequences in introns 1, 2, 4 and 10, and shorter sequences in introns 6, 11 and 14, than the corresponding
rice gene.
Received: 5 June 1998 / Accepted: 29 September 1998 相似文献
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Giorgia Romina Riboldi Tunnicliffe Gernot Gloeckner Greg S. Elgar Sydney Brenner André Rosenthal 《Mammalian genome》2000,11(3):213-219
The Japanese pufferfish Fugu rubripes with a genome of about 400 Mb is becoming increasingly recognized as a vertebrate model organism for comparative gene analysis
(see Elgar 1996 for review). We have isolated and sequenced two Fugu cosmids spanning a genomic region of 66 kb containing the Fugu homolog to the human PCOLCE-I (Gl?ckner et al. 1998). We then examined if RUMMAGE-DP, a newly developed analysis tool for
gene discovery which was designed for human and mouse genomic DNA, can be used for automatic annotation of Fugu genomic sequence. The exon prediction programs contained in RUMMAGE-DP performed better overall for the human sequence than
for the Fugu contig. The GENSCAN program was the only exon prediction programme that performed equally well for both organisms. We show
that RUMMAGE-DP is very useful in automatic analysis of Fugu sequences. Comparative analysis of the genomic structure of the PCOLCE-I genes in Fugu and human reveals that the exon/intron structure throughout the protein coding region is almost identical. We defined an
additional domain based on the high degree of similarity of 26 aa between mammals and Fugu. The PCOLCE-I protein in both organisms contains two highly conserved CUB domains. Exons 6 and 7 are the only coding exons
that differ in length between the two species. We assume that these exons do not code for any catalytic domain of the protein.
Analysis of the remaining five Fugu genes within the 66 kb interval revealed no conserved synteny with the corresponding human 7q22 region.
Received: 13 October 1998 / Accepted: 25 July 1999 相似文献
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The human NRAMP1 gene located on Chromosome (Chr) region 2q35 is a candidate gene for increased risk of infection by several
intracellular macrophage parasites, including M. tuberculosis and M. leprae. In search for a possible mutational hot spot, we have analyzed a 3.5-kb region 5′ to NRAMP1 that is highly enriched for DNA
repeat sequences. The repeat sequences could be grouped into one Mer element and six Alu elements, representing five Alu subfamilies,
that had integrated in the same DNA region during successive rounds of Alu retropositional activity. Comparative sequence
analysis of the Alu cluster region in humans, chimpanzee (Pan paniscus), and gorilla (Gorilla gorilla) revealed only modest sequence variability and failed to detect any evidence for genomic instability of the highly repetitive
DNA region. These results show that sequence length variants in the Alu-flanking regions as well as nucleotide substitutions
are the most common genomic variations even in a region of extreme Alu-clustering. Moreover, the high degree of sequence conservation
among three primate species argues against the Alu cluster being the site of frequent genomic rearrangements or other frequent
genetic events that might influence NRAMP1 expression.
Received: 20 September 1997 / Accepted: 23 January 1998 相似文献
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