Waterlogging responses of Sporobolus virginicus (L.) Kunth.

Summary Flooding responses in Sporobolus virginicus (L.) Kunth., a perennial C₄ grass, propagated from plants collected on the fringes of a mangrove swamp, were examined in a glasshouse study over 42 days. Flooding significantly reduced soil redox potential, induced adventitious root development, shifted resource allocation from below- to above-ground components without affecting total biomass accumulation and significantly decreased below-ground/above-ground biomass ratios. Although soil waterlogging significantly increased alcohol dehydrogenase activity (ADH) after 30 h, significant increase in central air space by 45–50% of the cross-sectional stem area eliminated root hypoxia, and ADH activity decreased to levels equivalent to drained controls after 42 days. In addition, flooded plants exhibited significantly higher carbon dioxide assimilation rates but similar relative growth rates (RGR) to drained controls. The results indicate that S. virginicus responds to water-logging by a combination of metabolic, morphological and anatomical mechanisms, which may account for its widespread distribution in coastal lagoons, estuaries and marshes.     

Plant biomass and nutrient levels of a tropical macrophyte (Cyperus papyrus L.) receiving domestic wastewater

The aboveground biomass and nutrient content ofCyperus papyrus were determined in a small tropical swamp receiving domestic wastewater. It was found that the biomass (4,955 g.m^–2 dry weight) was the highest ever reported for papyrus. The levels of both nitrogen and phosphorus in the plant organs were very high. The nitrogen concentrations of the various plant organs were 4.8% roots, 8.4% rhizomes, 4.5% scales, 4.8% culms, and 6.2% umbels on dry weight basis. As to phosphorus the concentrations were 0.09% roots, 0.11% rhizomes, 0.09% scales, 0.10% culms, and 0.13% umbels. The high biomass and nutrient contents of the plants may have been caused by the high nutrient levels in the surrounding water. Comparison of the nitrogen to phosphorus ratios in the plants to those in the surrounding water showed that the plants stored very high amounts of nitrogen.     

Seasonal flooding,soil salinity and primary production in northern prairie marshes

Hydrologic regime is an important control of primary production in wetland ecosystems. I investigated the coupling of flooding, soil salinity and plant production in northern prairie marshes that experience shallow spring flooding. Field experiments compared whitetop (Scolochloa festucacea) marsh that was: (1) nonflooded, (2) flooded during spring with 25 cm water and (3) nonflooded but irrigated with 1 cm water · day^–1. Pot culture experiments examined whitetop growth response to salinity treatments. The electrical conductivity of soil interstitial water (EC_e) at 15 cm depth was 4 to 8 dS· m^–1 lower in flooded marsh compared with nonflooded marsh during 2 years. Whitetop aboveground biomass in flooded marsh (937 g · m^–2, year 1; 969 g · m^–2, year 2) exceeded that of nonflooded marsh (117 g · m^–2 year 1; 475 g · m^–2, year 2). Irrigated plots had lower EC_e and higher aboveground biomass than nonflooded marsh. In pot culture, EC_e of 4.3 dS · m^–1 (3 g · L^–1 NaCl) reduced total whitetop biomass by 29 to 44% and EC_e of 21.6 dS · m^–1 (15 g · L^–1 NaCl) reduced biomass by more than 75%. Large reductions of EC_e and increases of whitetop growth with irrigation indicated that plants responded to changes in soil salinity and not other potential environmental changes caused by inundation. The results suggest that spring flooding controls whitetop production by decreasing soil salinity during spring and by buffering surface soils against large increases of soil salinity after mid-summer water level declines. This mechanism can explain higher marsh plant production under more reducing flooded soil conditions and may be an important link between intermittent flooding and primary production in other wetland ecosystems.     

Growth responses to waterlogging and drainage of woody Hakea (Proteaceae) seedlings,originating from contrasting habitats in south-western Australia

We investigated the responses of seven woody Hakea (Proteaceae) species (two populations of each), to two months of waterlogging and subsequent drainage, in a controlled glasshouse experiment. The species originated from contrasting environments (winter-wetland versus non-wetland habitats), and differed in abundance (endangered ironstone species versus common species). Waterlogging arrested growth of the main root system, and stimulated the formation of superficial adventitious roots just below the root/shoot junction in all species. Wetland species produced at least twice the amount of adventitious root dry mass of that of non-wetland species, due to differences in number, length or degree of branching. Their adventitious roots also tended to have higher porosities (7–10% versus 5–6% gas spaces). The relative amount of adventitious roots formed was strongly, positively correlated with the maintenance of shoot growth, and only the non-wetland species showed significant shoot growth reductions (19%) upon waterlogging. Dry mass percentage of stems and leaves, and leaf dry mass per area (LMA) increased considerably during waterlogging in all species (averages of 15, 29 and 27%, respectively), returning to the values of continuously drained control plants after drainage. Similarly, upon drainage, a suppression of shoot growth (average 35%) and a stimulation of root growth (average 50%) restored the root mass ratios to those of control plants. We found a negative correlation between the maintenance of growth during waterlogging versus that after waterlogging, suggesting a trade-off in functioning of the superficial adventitious roots between waterlogged and drained conditions. The rare winter-wet ironstone endemics resembled the common winter-wet species in most of their responses to waterlogging and drainage. Therefore, the results presented here cannot offer an explanation for their different distribution patterns. Our results suggest that non-wetland species may be disadvantaged in a wetland environment, due to their lower capacity to form adventitious roots resulting in stronger growth reductions.     

Effects of salinity and nitrogen on growth,ion relations and proline accumulation in Triglochin bulbosa

The effects of salinity and nitrogen on growth, ion relations and prolineaccumulation in the monocotyledonous halophyte, Triglochin bulbosa,was investigated in hydroponic culture over 5 months. The experimentaldesign was a 3 × 3 factorial with three salinity treatments (0, 150 and 300 mol m^-3 NaCl) and three levels of N (5, 10 and 20 gml^-1 N as NaNO₃). Total and root dry biomass accumulationwere significantly affected by salinity, but not by N or N × salinityinteraction. Increase in NaCl from 0 to 150 mol m^-3 had no effecton total or root dry biomass, while further increase in salinity to 300mol m^-3 significantly reduced biomass by 21% and 25%respectively. Shoot dry biomass, which was significantly affected by N andnot by salinity, increased with increase in N from 5 to 10 gml^-1. Ion concentrations in roots and shoots were significantlyaffected by salinity, but not by N or N × salinity interaction. Theconcentration of Na⁺ and Cl^- in roots and shoots increasedprogressively with an increase in salinity, while that of K⁺ decreased. Under non-saline conditions, Na⁺/K⁺ ratios were low (0.41to 0.44) and increased significantly with an increase in salinity in both rootsand shoots. Shoot sap osmotic potentials decreased progressively with anincrease in salinity. Increase in N in the hydroponic solution from 5 to20 g ml^-1 significantly increased root and shoot N by 66%and 41% respectively. Tissue concentrations of proline were significantlyaffected by salinity and substrate N but not by N × salinity interaction. Theconcentration of proline in roots and shoots increased significantly by334% and 48%, respectively, with an increase in salinity from 0 to 300mol m^-3 NaCl. Increase in substrate N from 5 to 20 g ml^-1 significantly increased proline in roots and shoots by 66% and41% respectively. The significance of substrate N on the accumulationof proline is discussed in relation to salt tolerance.     

Salinity effect on plant growth and leaf demography of the mangrove, Avicennia germinans L.

We assessed the effect of salinity on plant growth and leaf expansion rates, as well as the leaf life span and the dynamics of leaf production and mortality in seedlings of Avicennia germinans L. grown at 0, 170, 430, 680, and 940 mol m⁻³ NaCl. The relative growth rates (RGR) after 27 weeks reached a maximum (10.4 mg g⁻¹ d⁻¹) in 170 mol m⁻³ NaCl and decreased by 47 and 44% in plants grown at 680 and 940 mol m⁻³ NaCl. The relative leaf expansion rate (RLER) was maximal at 170 mol m⁻³ NaCl (120 cm m⁻² d⁻¹) and decreased by 57 and 52% in plants grown at 680 and 940 mol m⁻³ NaCl, respectively. In the same manner as RGR and RLER, the leaf production (P) and leaf death (D) decreased in 81 and 67% when salinity increased from 170 to 940 mol m⁻³ NaCl, respectively. Since the decrease in P with salinity was more pronounced than the decrease in D, the net accumulation of leaves per plant decreased with salinity. Additionally, an evident increase in annual mortality rates (λ) and death probability was observed with salinity. Leaf half-life (t _0.5) was 425 days in plants grown at 0 mol m⁻³ NaCl, and decreased to 75 days at 940 mol m⁻³ NaCl. Thus, increasing salinity caused an increase in mortality rate whereas production of new leaves and leaf longevity decreased and, finally, the leaf area was reduced.     

Effect of broken dead culms of Phragmites australis on radial oxygen loss in relation to radiation and temperature

The amount of oxygen released from the roots of Phragmites australis was quantified to examine the effects of airflow through dead culms, radiation, and temperature on radial oxygen loss (ROL). To investigate the effect of dead culms on ROL quantitatively, the ROL of individual plants with open dead culms was compared to that of plants with sealed dead culms as a function of light intensity and temperature. The relationship between ROL and plant morphology (aboveground biomass, shoot diameter, shoot height) was investigated. When exposed to 300, 600, and 900 μmol m⁻² s⁻¹ light, the ROL was 15.6, 22.5, and 30.9 μmol O₂ g⁻¹ dry root day⁻¹, respectively, from plants with open dead culms and 11.0, 16.4, and 23.3 μmol O₂ g⁻¹ dry root day⁻¹, respectively, from plants with sealed dead culms. The ROL from plants with open dead culms was obviously higher than that from plants with sealed dead culms in every condition. The ROL from plants with open culms was 37% and 30% higher than that from plants with sealed culms at 20°C and 30°C, respectively. The effects of plant-specific parameters such as leaf area and shoot diameter on radial oxygen loss were evident. From the point of view of rhizosphere oxidation during the growing season, the existence of open dead culms should be taken into consideration for optimal plant management in constructed wetlands. This study provides a theoretical understanding of the effects of open dead culms, light conditions, and temperature on radial oxygen loss. Handling editor: S. M. Thomaz     

Effects of short-term salinity on leaf gas exchange of the fig (Ficus carica L.)

The influence of short-term salinity (day 1–day 2: 50 mol m^–3 NaCl, day 3–day 7: 100 mol m^–3 NaCl in the nutrient solution) on leaf gas exchange characteristics were studied in two fig clones (Ficus carica L.), whose root mass had been varied in relation to the leaf area. The stomatal conductance was diminished by NaCl in the first week of treatment. NaCl slightly reduced the calculated intercellular partial pressure of CO₂. The net photosynthetic rate of plants with many roots was stimulated by NaCl on some days of the first week of treatment, whereas the net assimilation rate of the plants with few roots remained unaltered or decreased by NaCl. Only the assimilation of the salt-treated plants of one clone for some days during the first week of treatment seemed to be influenced by stomatal conductance. Nonstomatal factors were primarily responsible for the changes in CO₂ uptake in response to salt and/or root treatment. The water use efficiency increased during several days of the first week of NaCl treatment. Decreased stomatal conductance, increased water use efficiency and stimualtion of the net CO₂ assimilation rate appear to enhance salt tolerance during the first few days of salinity. ei]H Lambers     

Growth responses of individual roots of Opuntia ficus-indica to salinity

Responses of individual roots of the widely cultivated cactus Opuntia ficus-indica to salinity stress were evaluated using a split-root system. Three roots from a plant with at least 20 roots were isolated from the remainder of the root system and exposed to 0, 30 or 100 mol m^-3 NaCl for up to 28 d. Cortical cells became shorter and lateral root development was substantially reduced as salinity increased. Compared with the control, the increase in dry weight for the isolated roots was reduced 40% by 30 mol m^-3 NaCl and 93% by 100mol m^-3 NaCl. The sodium content of roots increased only two-fold with increasing salinity. Respiration rates of roots exposed to 30 or 100 mol m^-3 NaCl were higher than those of the control. Carbon accumulation in roots measured 2 d after exposing shoots to ¹⁴CO₂ was not initially affected by 30 mol m^-3 NaCl but was substantially reduced at 100 mol m^-3 NaCl. Thus, roots exposed to short periods of moderate salinity stress maintained sufficient carbon sink strength for continued growth of the roots. Moreover, increased salinity led to decreased efficiency of carbon usage for the expansion of root surface area.     

Responses of the saltmarsh rush Juncus kraussii to salinity and waterlogging

Juncus kraussii Hochst., an important saltmarsh macrophyte, is intensively harvested for many commercially orientated products and current populations are under threat of overexploitation. In saline, intertidal mud banks, this species occurs on higher ground, suggesting that it is adapted to lower salinities and less frequent inundation. The objectives of this study were to determine biomass accumulation, as well as morphological and physiological adaptations of J. kraussii to salinity and waterlogging stresses. Plants collected from the field were subjected to 0.2, 10, 30, 50 and 70% seawater under drained or flooded conditions for three months. Measurements were made of biomass accumulation, CO₂ exchange, chlorophyll fluorescence, ion and water relations. Furthermore, seed germination responses to a range of salinities were investigated. Total dry biomass accumulation, as well as the number and height of culms, decreased with increase in salinity under both flooded and drained conditions. Generally, CO₂ exchange, stomatal conductance, Photosystem II (PSII) quantum yield and electron transport rate (ETR) through PSII declined with increase in salinity in both the flooded and drained treatments. Predawn and midday ψ in culms decreased with increase in salinity, being lower under drained than flooded conditions. Inorganic solute concentrations in culms increased with increase in salinity, with Na⁺ and Cl⁻ being the predominant ions. Na⁺/K⁺ ratios in culms increased significantly with increase in salinity. Proline concentrations in roots and culms, which increased with salinity, were considerably higher under drained than flooded conditions. Germination was best at salinities less than 20% seawater and decreased significantly with further increase in salinity to 110% seawater. Transfer of ungerminated salt-treated seeds to distilled water stimulated germination. This study has demonstrated that J. kraussii is a highly salt and flood tolerant species, being able to grow and survive in salinities up to 70% seawater, under both drained and flooded conditions. Maximal growth occurred at low salinities (<10% seawater) under flooded condition.     

ANATOMICAL AND METABOLIC RESPONSES TO WATERLOGGING AND SALINITY IN SPARTINA ALTERNIFLORA AND S. PATENS (POACEAE)

The effects of waterlogging and salinity (25 or 325 mol m ³ NaCl) stressors on the anatomy and metabolism of the marsh grasses 5. alterniflora Loisel. and S. patens Aiton (Muhl.) were investigated in a V factorial greenhouse experiment over 30 d. Waterlogging and salinity in combination resulted in anatomical and metabolic responses in both species. Waterlogging reduced soil redox potential and decreased root-specific gravity significantly in both species. The inadequacy of aerenchyma development under hypoxia to support aerobic root respiration in S. patens was indicated by significant increases in root alcohol dehydrogenase (ADH) activity of 1,752% and 420%, respectively, in the low and high salinity treatments. ADH activity was not increased significantly by flooding of S. alterniflora. Proline concentrations in roots and leaves were low at low salinities and increased significantly at high salinities in both species, but only under drained conditions. Decrease in leaf elongation by high salinity occurred in drained, but not flooded treatments in both species. Under flooded conditions, leaf elongation was significantly greater in S. alterniflora than S. patens. Greatest leaf elongation occurred in flooded low salinity S. alterniflora plants that had the least proline. Although both species are adapted to waterlogging and salinity, S. alterniflora appears to be more tolerant of reducing soil conditions and less responsive to higher salinity than S. patens.     

Combined effect of salinity and hypoxia in wheat (Triticum aestivum L.) and wheat-Thinopyrum amphiploids

The effects of sodium chloride salinity and hypoxia were studied in eight wheat lines and three wheat-Thinopyrum amphiploids in vermiculite-gravel culture. The lines were treated with either 100 or 150 mol m^–3 NaCl with and without hypoxia. Saline hypoxic conditions significantly reduced the vegetative growth, water use, grain and straw yields for all wheat varieties except the amphiploids, whereas NaCl or hypoxia alone had less pronounced effects. In addition, saline hypoxic stress reduced K⁺ concentration and increased significantly the Na⁺ and Cl^– concentrations in cell sap expressed from leaves. There was more Na⁺ and Cl^– accumulation in wheats than the amphiploids in hypoxic conditions at 150 mol m^–3 NaCl. Of the wheats, Pato was the most sensitive at all stress levels while aTriticum aestivum cv. Chinese Spring ×Thinopyrum elongatum amphiploid was the most tolerant of the three amphiploids.     

Ecophysiological characterization of carnivorous plant roots: Oxygen fluxes,respiration, and water exudation

Various ecophysiological investigations on carnivorous plants in wet soils are presented. Radial oxygen loss from roots of Droseraceae to an anoxic medium was relatively low 0.02 – 0.07 mol(O₂) m^{– 2} s^–1 in the apical zone, while values of about one order of magnitude greater were found in both Sarracenia rubra roots and Genlisea violacea traps. Aerobic respiration rates were in the range of 1.6 – 5.6 mol kg^–1 (f.m.) s^–1 for apical root segments of seven carnivorous plant species and 0.4 – 1.1 mol kg^–1 (f.m.) s^–1 for Genlisea traps. The rate of anaerobic fermentation in roots of two Drosera species was only 5 – 14 % of the aerobic respiration. Neither 0.2 mM NaN₃ nor 0.5 mM KCN influenced respiration rate of roots and traps. In all species, the proportion of cyanide-resistant respiration was high and amounted to 65 – 89 % of the total value. Mean rates of water exudation from excised roots of 12 species ranged between 0.4 – 336 mm 3 kg^–1 (f.m.) s^–1 with the highest values being found in the Droseraceae. Exudation from roots was insensitive to respiration inhibitors. No significant difference was found between exudation rates from roots growing in situ in anoxic soil and those kept in an aerated aquatic medium. Carnivorous plant roots appear to be physiologically very active and well adapted to endure permanent soil anoxia.     

Root-zone temperature and salinity: Interacting effects on tillering,growth and element concentration in barley

The effects of root-zone salinity (0, 30, and 60 mmol L^–1 of NaCl) and root-zone temperature (10, 15, 20, and 25°C) and their interactions on the number of tillers, total dry matter production, and the concentration of nutrients in the roots and tops of barley (Hordeum vulgare L.) were studied. Experiments were conducted in growth chambers (day/night photoperiod of 16/8 h and constant air temperature of 20°C) and under water-culture conditions. Salinity and root temperature affected all the parameters tested. Interactions between salinity and temperature were significant (p<0.05) for the number of tillers, growth of tops and roots, and the concentration of Na, K, P in the tops and the concentration of P in the roots. Maximum number of tillers and the highest dry matter were produced when the root temperature was at the intermediate levels of 15 to 20°C. Effect of salinity on most parameters tested strongly depended on the prevailing root temperature. For example, at root temperature of 10°C addition of 30 mmol L^–1 NaCl to the nutrient solution stimulated the growth of barley roots; at root temperature of 25°C, however, the same NaCl concentration inhibited the root growth. At 60 mmol L^–1, root and shoot growth were maximum when root temperature was kept at the intermediate level of 15°C; most inhibition of salinity occurred at both low (10°C) and high (25°C) root temperatures. As the root temperature was raised from 10 to 25°C, the concentration of Na generally decreased in the tops and increased in the roots. At a given Na concentration in the tops or in the roots, respective growth of tops or roots was much less inhibited if the roots were grown at 15–20°C. It is concluded that the tolerance of barley plant to NaCl salinity of the rooting media appears to be altered by the root temperature and is highest if the root temperature is kept at 15 to 20°C.     

Effects of temperature, salinity and irradiance on the growth of the red tide dinoflagellate Gyrodinium instriatum Freudenthal et Lee

The effects of temperature, salinity and irradiance on the growth of the red tide dinoflagellate Gyrodinium instriatum Freudenthal et Lee were examined in the laboratory. Exposed to 45 different combinations of temperature (10–30 °C) and salinity (0–40) under saturating irradiance, G. instriatum exhibited its maximum growth rate of 0.7 divisions/day at a combination of 25 °C and a salinity of 30. Optimum growth rates (>0.5 divisions/day) were observed at temperatures ranging from 20 to 30 °C and at salinities from 10 to 35. The organism could not grow at ≤10 °C. In addition, G. instriatum burst at a salinity of 0 at all temperatures, but grew at a salinity of 5 at temperatures between 20 and 25 °C. It is noteworthy that G. instriatum is a euryhaline organism that can live under extremely low salinity. Factorial analysis revealed that the contributions of temperature and salinity to its growth of the organism were almost equal. The irradiance at the light compensation point (I₀) was 10.6 μmol/(m² s) and the saturated irradiance for growth (I_s) was 70 μmol/(m² s), which was lower than I_s for several other harmful dinoflagellates (90–110 μmol/(m² s)).     

Relative importance of Na+, Cl−, and abscisic acid in NaCl induced inhibition of root growth of rice seedlings

The relative importance of endogenous abscisic acid (ABA), as well as Na⁺ and Cl^– in NaCl-induced responses related to growth in roots of rice seedlings were investigated. The increase in ammonium, proline and H₂O₂ levels, and cell wall peroxidase (POD) activity has been shown to be related to NaCl-inhibited root growth of rice seedlings. Increasing concentrations of NaCl from 50 to 150 mM progressively decreased root growth and increased both Na⁺ and Cl^–. Treatment with NaCl in the presence of 4,4-diisothiocyano-2,2-disulfonic acid (DIDS, a nonpermeating amino-reactive disulfonic acid known to inhibit the uptake of Cl^–) had less Cl^– level in roots than that in the absence of DIDS, but did not affect the levels of Na⁺, and responses related to growth in roots. Treatment with 50 mM Na-gluconate (the anion of which is not permeable to membrane) had similar Na⁺ level in roots as that with 100 mM NaCl. It was found that treatment with 50 mM Na-gluconate effected growth reduction and growth-related responses in roots in the same way as 100 mM NaCl. All these results suggest that Cl^– is not required for NaCl-induced responses in root of rice seedlings. Endogenous ABA level showed no increase in roots of rice seedlings exposed to 150 mM NaCl. It is unlikely that ABA is associated with NaCl-inhibited root growth of rice seedlings.     

Standing crop and aboveground biomass partitioning of a dwarf mangrove forest in Taylor River Slough,Florida

The structure and standing crop biomass of a dwarf mangrove forest, located in the salinity transition zone ofTaylor River Slough in the Everglades National Park, were studied. Although the four mangrove species reported for Florida occurred at the study site, dwarf Rhizophora mangle trees dominated the forest. The structural characteristics of the mangrove forest were relatively simple: tree height varied from 0.9 to 1.2 meters, and tree density ranged from 7062 to 23 778 stems ha^–1. An allometric relationship was developed to estimate leaf, branch, prop root, and total aboveground biomass of dwarf Rhizophora mangle trees. Total aboveground biomass and their components were best estimated as a power function of the crown area times number of prop roots as an independent variable (Y = B × X^–0.5083). The allometric equation for each tree component was highly significant (p<0.0001), with all r² values greater than 0.90. The allometric relationship was used to estimate total aboveground biomass that ranged from 7.9 to 23.2 ton ha^–1. Rhizophora mangle contributed 85% of total standing crop biomass. Conocarpus erectus, Laguncularia racemosa, and Avicennia germinans contributed the remaining biomass. Average aboveground biomass allocation was 69% for prop roots, 25% for stem and branches, and 6% for leaves. This aboveground biomass partitioning pattern, which gives a major role to prop roots that have the potential to produce an extensive root system, may be an important biological strategy in response to low phosphorus availability and relatively reduced soils that characterize mangrove forests in South Florida.     

Seasonal dynamics of <Emphasis Type="Italic">Zostera noltii</Emphasis> Hornem. in two Mediterranean lagoons

The density, biomass and shoot morphology of two populations of Zostera noltii were monitored from January 1998 to July 1999 at two shallow Mediterranean lagoons of Biguglia and Urbino, which differ in hydro-morphological conditions and nutrient loading. Monitoring included the principal biological and foliar parameters (shoot density, aboveground and belowground biomass, length, width and number of leaves, LAI and coefficient A: percentage of leaves having lost their apex), the organic matter contents of the sediment and the environmental conditions (salinity, turbidity, temperature, nutrient concentrations and dissolved oxygen levels). The two populations of Z. noltii displayed seasonal changes in density (1600–19600 m²), aboveground biomass (11–153 g. DW. m⁻²), leaf length (33–255 mm), and leaf width (0.9–1.8 mm). Temperature and turbidity were significant environmental factors influencing the temporal changes observed in the Z. noltii meadows studied. Conversely, the belowground biomass, the number of leaves per shoot and the LAI did not undergo any seasonal changes. In the Biguglia lagoon, the functioning dynamics of the Z. noltii seagrass beds are determined by the catchment area and the inputs of nutrients derived from it, whereas in the Urbino lagoon the dynamics of the Z. noltiibeds depend on low levels of water turbidity.     

Annual and seasonal changes in fine root biomass of a Quercus ilex L. forest

The biomass, production and mortality of fine roots (roots with diameter <2.5 mm) were studied in a typical Mediterranean holm oak (Quercus ilex L.) forest in NE Spain using the minirhizotron methodology. A total of 1212 roots were monitored between June of 1994 and March of 1997. Mean annual fine root biomass in the holm oak forest of Prades was 71±8 g m^–2 yr^–1. Mean annual production for the period analysed was 260+11 g m^–2 yr^–1. Mortality was similar to production, with a mean value of 253±3 g m^–2 yr^–1. Seasonal fine root biomass presented a cyclic behaviour, with higher values in autumn and winter and lower in spring and summer. Production was highest in winter, and mortality in spring. In summer, production and mortality values were the lowest for the year. Production values in autumn and spring were very similar. The vertical distribution of fine root biomass decreased with increasing depth except for the top 10–20 cm, where values were lower than immediately below. Production and mortality values were similar between 10 and 50 cm depth. In the 0–10 cm and the 50–60 cm depth intervals, both production and mortality were lower.     

Root biomass changes after drainage and fertilization of a low-shrub pine bog

The stump and root systems of Scots pine (Pinus sylvestris) and field-layer vegetation were sampled before (1984) and three growing seasons after drainage and fertilization (1987) of a low-shrub pine bog. Average below-ground biomass of the field layer was 548 gDW m^–2 in 1984, with no significant treatment effects during experimentation. The stump-plus-root biomass of the pine stands was 1464 gDW m^–2 in the virgin state, and had increased to 1854 gDW m^–2 three years after the NPK-fertilizer treatment. The distribution over fractions also changed with this treatment. The fraction of fine roots ( < 1 mm) in stump-root biomass increased from 4% (56 gDW m^–2) to 11% (196 gDW m^–2), while the other compartments changed less. Total pine root length was 729 mm^–2 in 1984. Root length increased by 94% to 1380 mm^–2 on NPK-fertilized plots. Most of the fine pine roots were in the surface layer (0–10 cm), 79% in 1984 and 88% in 1987, and few pine roots were deeper than 20 cm. Maximum root length of fine pine roots ( < 1 mm) was estimated to be 2710 mm^–2 at about 800 gDW m^–2 (NPK treatment), and the corresponding maximum for small pine roots (=1–10 mm) was 227 mm^–2 at 809 gDW m^–2. Drainage stimulated net growth of fine roots, but this treatment also caused higher mortality rates of small roots. The fine roots responded to fertilization with higher net growth rate, and secondary growth of the large roots ( > 10 mm) was improved. The observed changes in root biomass and structure are explained as strategic adaptations to altered hydrological and nutritional circumstances in the root zone after drainage and fertilization.