共查询到20条相似文献,搜索用时 15 毫秒
1.
植物耐盐的分子生物学基础 总被引:8,自引:0,他引:8
植物分子水平的耐盐研究是近年的研究热点,通过综述与耐盐有关的几种重要分子的性质和作用,总结了几种与植物耐盐有关的基因以及它们在盐分胁迫下的表达和调控。 相似文献
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The halobacteria are the only organisms that are tolerant of salinity at
the molecular level. All other bacteria, all fungi, all plants, and all
animals avoid the need for salt tolerance for most of their macromolecules
by maintaining defined and conserved conditions in the cytoplasm. These
conditions favour potassium over sodium, the limitation of total inorganic
ion activity, and the supplementation of this where necessary with organic
solutes which are metabolically neutral osmolytes that may also be
osmoprotectant. The salt tolerance of an organism depends upon the range of
external salinity over which it is able to sustain these conditions in the
cytoplasm. There is substantial and increasing knowledge of the molecular
biology and molecular genetics of the processes of ion and organic solute
transport, solute synthesis, and compartmentation that underpin cell-based
tolerance. Much of recent research focuses on the identification of genes
and gene products that affect cell-based tolerance, commonly derived from
single-cell models. There is commonly the implicit or explicit assumption
that incorporation of these genes will benefit the salt tolerance of food
crop species. While this essential experimental approach is giving enormous
insight there should not be rash or premature expectations. The unique and
overriding consideration for the salinity tolerance of terrestrial plants
is the net flux of water due to transpiration and so resides at a higher
level of organization. Processes that are advantageous to a single cell in
an aqueous medium may be lethal to a cell in a leaf in the air. The likely
impact of single structural-gene changes in ion and solute transport upon
co-ordinated plant response is probably over-estimated, and recent views
consider regulatory processes and multiple gene transfers. While the
technical ability for plant transformation increases daily, the
practicality of using transgenic plants in complex breeding programmes
seems rarely to be given enough thought. If intervention at the molecular
level is to lead to salt-tolerant crop plants than it will be essential to
view this in the contexts of whole plants and of plant breeding. Recent
indications that a relatively small number of quantitative trait loci (QTL)
may govern complex physiological characters offer the most hope for the
future. 相似文献
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耐盐酶在高盐浓度下仍具备催化活性和稳定性,在高盐食品和海产品加工、洗涤及其它高盐环境生物技术领域被广泛应用;耐盐基因在高盐条件下可以使微生物维持正常功能,获取并研究不同环境中的耐盐基因对揭示微生物的耐盐机制,以及实现其在高盐环境中的定向应用具有的重要意义。宏基因组学避开纯培养技术探知微生物的多样性及其功能,为我们提供了一种发现新基因、开发新的微生物活性物质和研究微生物群落结构及其功能的新技术。文中结合本课题组的研究工作,综述了利用宏基因组学获取耐盐酶类及耐盐基因的策略,同时着重介绍利用宏基因组学从海洋、土壤、胃肠道等环境中获取耐盐酶类及耐盐基因的研究。 相似文献
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Plant salt tolerance 总被引:156,自引:0,他引:156
Zhu JK 《Trends in plant science》2001,6(2):66-71
Soil salinity is a major abiotic stress in plant agriculture worldwide. This has led to research into salt tolerance with the aim of improving crop plants. However, salt tolerance might have much wider implications because transgenic salt-tolerant plants often also tolerate other stresses including chilling, freezing, heat and drought. Unfortunately, suitable genetic model systems have been hard to find. A recently discovered halophytic plant species, Thellungiella halophila, now promises to help in the detection of new tolerance determinants and operating pathways in a model system that is not limited to Arabidopsis traits or ecotype variations. 相似文献
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Breeding tomatoes for salt tolerance: inheritance of salt tolerance and related traits in interspecific populations 总被引:3,自引:0,他引:3
Y. Saranga A. Cahaner D. Zamir A. Marani J. Rudich 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1992,84(3-4):390-396
Summary Interspecific segregating populations derived from a cross between tomato (Lycopersicon esculentum) cv M82-1 -8 (M82) and the wild species L. pennellii accession LA-716 (Lpen716) were used to study the genetic basis of salt tolerance and its implications for breeding. BC1 (M82 x (M82 x Lpen716)) and BC1 S1 (progenies of selfed BC1 plants) populations were grown under arid field conditions and irrigated with water having electrical conductivities of 1.5 (control), 10 and 20 dSm-1. The evaluation of salt tolerance was based on total fruit yield (TY), total dry matter (TD) and TD under salinity relative to the control (RD). Sodium, potassium and chloride concentrations were measured in the leaves and stems. The methods for estimating heritability were adapted to BC1 plants and BC1S1 families. TY, TD and RD had heritability estimates of 0.3–0.45, indicating that salt tolerance can be improved by selection. Genetic correlations between traits indicated that high yield may be combined with salt tolerance and that ion contents are not likely to provide an efficient selection criteria for salt tolerance. Genetic correlations between performances under various salinity levels suggested that similar mechanisms affect the responses to salinity treatments of 10 and 20 dSm-1. Responses to paper selection confirmed that salt tolerance of the tomato may be improved by selection, and that this selection should be based on dry matter and yield parameters under salinity.Passed away May 1986 相似文献
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As glycerol was suggested as an osmotic agent in the salt tolerantDebaryomyces hansenii the concentrations of total, intracellular, and extracellular glycerol produced by this yeast was followed during growth in 4 mM, 0.68 M, and 2.7 M NaCl media. The total amount of glycerol was not directly proportional to biomass production but to the cultural salinity with maximum concentrations just prior to or at the beginning of the stationary phase. In all cultures the cells lost some glycerol to the media, at 2.7 M NaCl the extracellular glycerol even amounted maximally to 80% of the total. A distinct maximum of intracellular glycerol, related to dry weight or cell number, appeared during the log phase at all NaCl concentrations. As the intracellular calculated glycerol concentrations amounted to 0.2 M, 0.8 M, and 2.6 M in late log phase cells at 4 mM, 0.68 M, and 2.7 M NaCl, respectively, whereas the corresponding analysed values for the glycerol concentrations of the media were 0.7 mM, 2.5 mM, and 3.0 mM, glycerol contributes to the osmotic balance of the cells.During the course of growth all cultures showed a decreasing heat production related to cell substance produced, most pronounced at 2.7 M NaCl. At 2.7 M NaCl the total heat production amounted to-1690 kJ per mole glucose consumed in contrast to-1200 and-1130 kJ at 4 mM and 0.68 M NaCl, respectively. TheY
m
-values were of an inverse order, being 129, 120, and 93 at 4 mM, 0.68 M, and 2.7 M NaCl, respectively. 相似文献
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Molecular characterization of functional domains in the protein kinase SOS2 that is required for plant salt tolerance 总被引:35,自引:0,他引:35 下载免费PDF全文
The SOS3 (for SALT OVERLY SENSITIVE3) calcium binding protein and SOS2 protein kinase are required for sodium and potassium ion homeostasis and salt tolerance in Arabidopsis. We have shown previously that SOS3 interacts with and activates the SOS2 protein kinase. We report here the identification of a SOS3 binding motif in SOS2 that also serves as the kinase autoinhibitory domain. Yeast two-hybrid assays as well as in vitro binding assays revealed a 21-amino acid motif in the regulatory domain of SOS2 that is necessary and sufficient for interaction with SOS3. Database searches revealed a large family of SOS2-like protein kinases containing such a SOS3 binding motif. Using a yeast two-hybrid system, we show that these SOS2-like kinases interact with members of the SOS3 family of calcium binding proteins. Two-hybrid assays also revealed interaction between the N-terminal kinase domain and the C-terminal regulatory domain within SOS2, suggesting that the regulatory domain may inhibit kinase activity by blocking substrate access to the catalytic site. Removal of the regulatory domain of SOS2, including the SOS3 binding motif, resulted in constitutive activation of the protein kinase, indicating that the SOS3 binding motif can serve as a kinase autoinhibitory domain. Constitutively active SOS2 that is SOS3 independent also was produced by changing Thr(168) to Asp in the activation loop of the SOS2 kinase domain. Combining the Thr(168)-to-Asp mutation with the autoinhibitory domain deletion created a superactive SOS2 kinase. These results provide insights into regulation of the kinase activities of SOS2 and the SOS2 family of protein kinases. 相似文献
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目的 通过分析植物乳杆菌PUM1785体外抑菌活性和部分耐受能力,为进一步研发乳杆菌微生态制剂提供理论和数据支持。 方法 以模式菌株WCSF1为对照株,采用双层琼脂点种法进行体外抑菌试验,并开展高胆盐、高盐环境耐受试验。 结果 植物乳杆菌PUM1785体外抑菌活性与模式菌株相近,对6种常见致病菌均有较强的抑制作用,对革兰阴性菌的抑菌效果优于革兰阳性菌。在不同浓度胆盐溶液中培养24 h后,2株乳杆菌生长均受抑制,当胆盐浓度从0 g/100 mL持续增至0.5 g/100 mL后,2株乳杆菌活菌数量呈下降趋势,但始终维持在105 CFU/mL数量级以上,并且PUM1785与WCSF1活菌数量比呈上升趋势;在不同浓度的NaCl溶液中培养24 h后,2株乳杆菌均生长良好,当NaCl浓度从0 g/100 mL升高到8 g/100 mL时,2株乳杆菌活菌数始终维持在108 CFU/mL数量级以上,并且PUM1785与WCSF1活菌数量比呈明显上升趋势。 结论 植物乳杆菌PUM1785具有与模式菌株相近的抑菌活性,对胆盐和高盐环境耐受力均强于模式菌株,表明PUM1785具有良好的生物学特性,可以作为微生态制剂研发的候选菌株。 相似文献
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Advances in salt tolerance 总被引:6,自引:0,他引:6
Summary Advances in and prospects for the development of salt tolerant crops are discussed. The genetic approach to the salinity problem
is fairly new, but research has become quite active in a short span of time. Difficulties and opportunities are outlined.
Salinity varies spatially, temporally, qualitatively, and quantitatively. In addition, the responses of plants to salt stress
vary during their life cycle. Selection and breeding, including the use of wide crosses, are considered the best short-term
approaches to the development of salt tolerant crops, but the new biotechnological and molecular biological techniques will
make increasingly important contributions. Cooperation is called for among soil and water scientists, agronomists, plant physiologists
and biochemists, cytologists, and plant geneticists, breeders, and biotechnologists. Given such cooperation and adequate support
for these endeavors, the potential for increasing productivity in salt-affected areas can be realized. 相似文献
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Improving crop salt tolerance 总被引:63,自引:0,他引:63
Flowers TJ 《Journal of experimental botany》2004,55(396):307-319
Salinity is an ever-present threat to crop yields, especially in countries where irrigation is an essential aid to agriculture. Although the tolerance of saline conditions by plants is variable, crop species are generally intolerant of one-third of the concentration of salts found in seawater. Attempts to improve the salt tolerance of crops through conventional breeding programmes have met with very limited success, due to the complexity of the trait: salt tolerance is complex genetically and physiologically. Tolerance often shows the characteristics of a multigenic trait, with quantitative trait loci (QTLs) associated with tolerance identified in barley, citrus, rice, and tomato and with ion transport under saline conditions in barley, citrus and rice. Physiologically salt tolerance is also complex, with halophytes and less tolerant plants showing a wide range of adaptations. Attempts to enhance tolerance have involved conventional breeding programmes, the use of in vitro selection, pooling physiological traits, interspecific hybridization, using halophytes as alternative crops, the use of marker-aided selection, and the use of transgenic plants. It is surprising that, in spite of the complexity of salt tolerance, there are commonly claims in the literature that the transfer of a single or a few genes can increase the tolerance of plants to saline conditions. Evaluation of such claims reveals that, of the 68 papers produced between 1993 and early 2003, only 19 report quantitative estimates of plant growth. Of these, four papers contain quantitative data on the response of transformants and wild-type of six species without and with salinity applied in an appropriate manner. About half of all the papers report data on experiments conducted under conditions where there is little or no transpiration: such experiments may provide insights into components of tolerance, but are not grounds for claims of enhanced tolerance at the whole plant level. Whether enhanced tolerance, where properly established, is due to the chance alteration of a factor that is limiting in a complex chain or an effect on signalling remains to be elucidated. After ten years of research using transgenic plants to alter salt tolerance, the value of this approach has yet to be established in the field. 相似文献
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Increasing salt tolerance in the tomato 总被引:9,自引:0,他引:9
In this paper, a number of strategies to overcome the deleterious effects of salinity on plants will be reviewed; these strategies include using molecular markers and genetic transformation as tools to develop salinity-tolerant genotypes, and some cultural techniques. For more than 12 years, QTL analysis has been attempted in order to understand the genetics of salt tolerance and to deal with component traits in breeding programmes. Despite innovations like better marker systems and improved genetic mapping strategies, the success of marker-assisted selection has been very limited because, in part, of inadequate experimental design. Since salinity is variable in time and space, experimental design must allow the study of genotype x environment interaction. Genetic transformation could become a powerful tool in plant breeding, but the growing knowledge from plant physiology must be integrated with molecular breeding techniques. It has been shown that the expression of several transgenes promotes a higher level of salt tolerance in some species. Despite this promising result, the development of a salt-tolerant cultivar by way of transgenesis has still not been achieved. Future directions in order to overcome the present limitations are proposed. Three cultural techniques have proved useful in tomato to overcome, in part, the effects of salinity: treatment of seedlings with drought or NaCl ameliorates the adaptation of adult plants to salinity; mist applied to tomato plants grown in Mediterranean conditions improves vegetative growth and yield in saline conditions; and grafting tomato cultivars onto appropriate rootstocks could reduce the effects of salinity. 相似文献
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Improving salt tolerance of economically important plants is imperative to cope with the increasing soil salinity in many
parts of the world. Mutation breeding has been widely used to improve plant performance under salinity stress. In this study,
we have mutagenized Echinochloa crusgalli L. with sodium azide and three selected mutants (designated fows A) with salt tolerant germination. Their vegetative growth was compared to that of the wild type after short-term and long-term
salt stress. The germination of the three fows A mutants in the presence of inhibitory concentrations of NaCl, KCL, and mannitol was better than that of the wild type.
Early growth of the mutants in the presence of 200 mM NaCl was also better than that of the wild type perhaps due to improved
K+ uptake and enhanced accumulation of sugars particularly sucrose at least in two mutants. But the three mutants and the wild
type responded similarly to long-term salt stress. The tolerance mechanisms during short-term and long-term salt stress are
discussed. 相似文献
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A method for screening rice plants for salt tolerance 总被引:5,自引:0,他引:5
A number of varieties of rice, a halophyte, Sesuvium portulacastrum and a glycophyte, Phaseolus vulgaris were grown in culture solution containing a range of concentrations of NaCl. Growth of the plants and internal sodium concentrations
of the roots were measured after 14 days. The electrical potential difference (PD) between the external solution and the vacuole
of the outer cells of the root was also measured. This enabled the driving force on sodium at the cell membranes to be calculated
using the Nernst equation. It was found that Sesuvium and those varieties of rice that had previously shown salt tolerance generated relatively negative PDs and large driving
forces tending to exclude sodium from the root. This suggested that a simple measurement of PD for plants grown in a given
concentration of NaCl over a given period of time would provide a fairly rapid screening method for salt tolerance in rice
and possibly other species also. T J Flowers Section editor 相似文献
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M. Ashraf 《Biologia Plantarum》1994,36(2):255-259
Responses of a salt tolerant and a normal population of an oilseed crop,Eruca sativa Mill. were assessed after four weeks growth in sand culture salinized with 0 (control), 100, 200, or 300 mol m?3 NaCl. The salt tolerant plants produced significantly greater dry biomass than the normal population. The populations did not differ significantly in leaf osmotic potential, relative water content and leaf soluble proteins. However, the tolerant population accumulated significantly greater amounts of soluble sugars, proline and free amino acids in the leaves compared with the non-tolerant population. It is established that leaf soluble sugars, proline, and free amino acids are important components of salt tolerance inEruca sativa. 相似文献
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The effects of KCl, NaCl, and LiCl on the growth of Debaryomyces hansenii, usually considered a halotolerant yeast, and Saccharomyces cerevisiae were compared. KCl and NaCl had similar effects on D. hansenii, indicating that NaCl created only osmotic stress, while LiCl had a specific inhibitory effect, although relatively weaker than in S. cerevisiae. In media with low K+, Na+ was able to substitute for K+, restoring the specific growth rate and the final biomass of the culture. The intracellular concentration of Na+ reached values up to 800 mM, suggesting that metabolism is not affected by rather high concentrations of salt. The ability of D. hansenii to extrude Na+ and Li+ was similar to that described for S. cerevisiae, suggesting that this mechanism is not responsible for the increased halotolerance. Also, the kinetic parameters of Rb+ uptake in D. hansenii (Vmax, 4.2 nmol mg [dry weight]-1 min-1; K(m), 7.4 mM) indicate that the transport system was not more efficient than in S. cerevisiae. Sodium (50 mM) activated the transport of Rb+ by increasing the affinity for the substrate in D. hansenii, while the effect was opposite in S. cerevisiae. Lithium inhibited Rb+ uptake in D. hansenii. We propose that the metabolism of D. hansenii is less sensitive to intracellular Na+ than is that of S. cerevisiae, that Na+ substitutes for K+ when K+ is scarce, and that the transport of K+ is favored by the presence of Na+. In low K+ environments, D. hansenii behaved as a halophilic yeast. 相似文献
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