中国异蚤蝇属一新种一新纪录种(双翅目:蚤蝇科)

记述了采自中国黑龙江的双翅目蚤蝇科1新种:柳叶异蚤蝇Megaselia lanceoseta sp.nov..本种主要鉴别特征为:中侧片具毛,缺鬃;背侧鬃3根,缺背侧裂;小盾片鬃2对;生殖背板两侧被鬃状毛;肛侧片每侧各具2根下弯的鬃;载肛片端鬃呈柳叶状.本文还在我国首次记录了阔径异蚤蝇Megaselia humeral...     

寄生白蚁的叉蚤蝇属中国首报及一新种记述(双翅目,蚤蝇科)(英文)

叉蚤蝇属Dicranopteron Schmitz种类均为白蚁的拟寄生物,目前世界记载6种。报道了该属首见于我国,并记述1新种扇毛叉蚤蝇D.flabellatum sp.nov.。扇毛叉蚤蝇与D.brevifurca Disney翅脉相似,但前者后足胫节背毛具扇状末端,极易区别。扇状毛也见于D.palmasetorum Disney,但两者的翅脉截然不同。模式标本存于沈阳大学城市有害生物治理与生态安全辽宁省重点实验室。     

中国喙蚤蝇属一新种（双翅目：蚤蝇科）

喙蚤蝇属ＴｒｏｐｈｉｔｈａｕｍａＳｃｈｍｉｔｚ是一形态特异的类群。其雌性种类口上片延长,喙长,腹部第４－６背板特化成腺体。目前全世界仅记载６种。我国只记载１种（刘广纯等,１９９３）。本文描述１新种。模式标本存于沈阳农业大学植保系昆虫标本室。黄腰喙蚤蝇ＴｒｏｐｈｉｔｈａｕｍａｇａｓｔｒｏｆｌａｖｉｄｕｍＬｉｕ,新种雌：体长２ｍｍ。额黑色,有光泽。触角第３节黑色,球形;芒０．６ｍｍ,具较长绒毛。下颚须黑色,具短鬃。胸背板和侧板下半部黑色;侧板下半部浅黄色。小盾片鬃ｌ对,短毛１对。腹部第１节浅黄,其它节黑色。第Ⅳ－Ⅵ背板高度特化。翅长１．８ｍｍ;前缘脉指数０．５８,各段比２：１．６：１,前缘脉纤毛０．０４ｍｍ。腋区鬃３根,平衡棒浅黄。前、中足浅褐,后足深褐。中足栅毛列达胫节２／３;后足胫节栅毛列完整,具后背纤毛列。正模：♀,云南勐养,１９９１－Ⅵ－８,刘广纯采。     

中国栓蚤蝇属(双翅目,蚤蝇科)一新种

记述了中国栓蚤蝇属Dohrniphora Dahl 1新种,即微刺栓蚤蝇D.microspinosa Liu et Shen,sp.nov.模式标本保存于沈阳大学生物与环境工程学院.     

中国寒蚤蝇属一新种记述（双翅目：蚤蝇科）

记述中国寒蚤蝇属Triphleba Rondani 1新种:壳叶寒蚤蝇Triphleba conchiformis sp. nov.。本新种缺R2+3,其贝壳状侧尾叶区别于本属其它种。模式标本存放于沈阳大学城市有害生物治理与生态安全辽宁省重点实验室。     

中国栓蚤蝇属一新种记述（双翅目：蚤蝇科）

记述采自海南的栓蚤蝇属Dohrniphora Dahl 1新种:密齿栓蚤蝇Dohrniphora densilinearis sp. nov.。新种与D. eilogoensis Disney相似,但后足腿节基部栓状感器大小相等且呈直线排列,可以区分。模式标本藏于沈阳大学城市有害生物治理与生态安全辽宁省重点实验室。     

中国广东异蚤蝇一新种记述(双翅目,蚤蝇科)

记述异蚤蝇属1新种:双鬃异蚤蝇Megaselia bisetalis sp.nov..模式标本存放于沈阳大学生物与环境工程学院.双鬃异蚤蝇,新种Megaselia bisetalis sp.nov.(图1～3)雄性额红褐至黑褐;额宽稍大于侧高;密布细毛;纵沟明显.触角上鬃4根,不等.前额间鬃几与触角上鬃同高.后额间鬃略低于后额框鬃,4根鬃等距排列.颊鬃2根,侧颜鬃5根.触角第3节黄褐色,端部褐;球形;芒具微毛.下颚须浅黄,具较长鬃.胸黄褐色,侧板略浅;小盾片 鬃1对,短毛1对;中侧片光裸.腹部腹面黄色;背板大部分黄褐色,尾器黑褐,生殖背板两侧各具长鬃1根.肛管黄色,端毛粗大.翅长1.04～1.10mm,前缘脉指数0.41 mm,各段比3.75∶1.75∶1.00.平衡棒黄褐.足黄色,前足胫节具前背刺1列;基跗节细长.中足胫节栅毛列达端部1/4,后背纤毛6根.后足胫节栅毛列完整,后背纤毛10根,缺前背纤毛.体长1.2mm.雌性体长1.2～1.6 mm.翅长1.3～1.4mm.前缘脉指数0.43,平衡棒褐色.腹背板褐色,腹面黄色.正模♂,广东顶湖,1992-04-24,刘广纯、王敏采;副模3♂♂,5♀♀,采集记录同正模.词源:新种种名据其尾器特点而拟.     

中国异蚤蝇属二新种记述（双翅目：蚤蝇科）

记述中国异蚤蝇属2新种:羽鬃异蚤蝇Megaselia pennisetalis sp.nov.和膨跗异蚤蝇Megaselia tarsocrassa sp.nov.。羽鬃异蚤蝇的体色和尾器的羽状鬃与M.rufipes(Meigen)相似,但后者的中侧片光裸无毛。膨跗异蚤蝇与M.turbidipennis Borgmeier相似,但后者翅较暗、Rs脉基部具1微毛、前缘脉比3.80:1.60:1.00。模式标本存放于沈阳大学城市有害生物治理与生态安全辽宁省重点实验室。     

中国乌蚤蝇属二新种(双翅目,蚤蝇科)(英文)

记述了中国乌蚤蝇属2新种,葫片乌蚤蝇Woodiphora lageniformis Liu et Zhu,sp.nov.和明带乌蚤蝇Woodiphora fasciaria Liu et Zhu,sp.nov.葫片乌蚤蝇第6背板内部小骨片呈葫芦形,第7背板向后明显变狭区别于其它种.明带乌蚤蝇以第4背板中部具1无色横条,第5背板长等于宽,几乎呈正方形区别于其它种.模式标本存于沈阳大学生物与环境工程学院.     

中国伐蚤蝇属一新种记述(双翅目,蚤蝇科)(英文)

记述了中国伐蚤蝇属1新种,尖尾伐蚤蝇Phalacrotophor acaudarguta sp.nov.,模式标本存于沈阳大学城市有害生物治理与生态安全辽宁省重点实验室。     

Genome complexity and organization in the red imported fire ant Solenopsis invicta Buren

The red imported fire ant Solenopsis invicta is the most destructive invading arthropod in the southern United States, yet little is known about its genome complexity and organization. Here we report the size, organization and GC content of S. invicta genome. DNA reassociation kinetics using S1 nuclease assay and a modified second-order kinetics model indicated that the S. invicta genome is approximately 0.62 picograms or 5.91 x 10(8) base pairs, composed of 36% unique, 41% moderately repetitive and 23% highly repetitive/foldback sequences. Comparison of the reassociation kinetics of short and long DNA fragments revealed that the sequence arrangement follows a pattern of short period interspersion, as in most organisms with relatively large genomes. Melting-temperature analysis showed that the GC content of the fire ant genomic DNA is 34.8%, similar to that of most eukaryotic organisms. The results reveal that the fire ant genome is much larger and more complex than those of a number of hymenopteran insects studied to date. Our study provides a foundation for further analysis and genetic manipulation of the S. invicta genome.     

红火蚁雌蚁生殖发育的研究

通过解剖多后型红火蚁Solenopsis invicta Buren雌蛹、有翅雌蚁和脱翅雌蚁,观察了雌蚁内生殖系统的结构和发育进度。结果表明,多后型雌蚁内生殖系统形成于蛹期,其结构与单后型雌蚁存在一定差异。从发育进度来看,虽然雌蚁羽化后其卵巢大小和卵巢管数量仍有一定增长,但蛹期是卵巢大小、卵巢管数量快速增长的阶段;卵子发生始见于羽化后第4d,且卵量随个体发育而快速增加,直至脱翅后第10d达到高峰,随后开始下降;生殖系统的其它结构的变化较小。雌蛹、有翅雌蚁和脱翅雌蚁发育后期的卵巢平均大小分别为1149．23×712．42、1594．88×834．26和1975．60×1303．57μm;而卵巢管数量则分别为62．67、81．33和86．33条。至雌蚁脱翅后10d,卵巢中成熟卵和总卵量分别为20．00±4．62粒和34．00±2．31粒。     

Revisiting the trail pheromone components of the red imported fire ant,Solenopsis invicta Buren

Ants use species-specific trail pheromones to coordinate their sophisticated foraging behavior. During the past decades, many trail pheromone components with various structures have been identified in ants, including the red imported fire ant, Solenopsis invicta, a notorious invasive species worldwide. Four compounds, Z,E- (ZEF) and E,E-α-farnesene (EEF), Z,E- (ZEHF) and E,E-α-homofarnesene (EEHF), have been reported as components of S. invicta trail pheromone. However, another study reported an analog of α-farnesene, Z,Z,Z-allofarnesene, as a key trail pheromone component. These contrasting results caused some uncertainty about the trail pheromone composition in S. invicta. In this study, we synthesized ZEF and EEF, ZEHF and EEHF, and reanalyzed the chemicals in the Dufour gland extract and in the trail pheromone fraction of S. invicta worker body extract. The reported isomers of farnesene and homofarnesene were detected and showed trail-following activity, with ZEF as the major compound, while no allofarnesene was found, neither in the Dufour gland extract nor in the whole-body extract. Our results confirm ZEF and EEF, ZEHF and EEHF as trail pheromone components of S. invicta.     

四种毒饵对红火蚁的田间防治效果评价

采用工蚁诱测法和挖巢观察法评价了4种国产毒饵对红火蚁Solenopsis invicta的田间防治效果。结果表明,氟虫胺、胺菊酯、苯氧威、多杀霉素4种毒饵处理后,红火蚁工蚁诱集百分率均明显降低,处理5d后氟虫胺、胺菊酯的工蚁诱集率为0,而多杀霉素处理10d后诱集率为0。施药后25d4种毒饵处理活动蚁巢减退率分别为100％、100％、50％和71．4％,工蚁减退率分别为100％、100％、92．7％和100％。处理6周后蚁群级别分别降低了100％、100％、83．3％、73．0％。     

Characteristics of 11 polymorphic microsatellite markers in the red imported fire ant, Solenopsis invicta Buren

We have characterized 11 polymorphic microsatellite loci in the invasive ant Solenopsis invicta. Primer pairs were evaluated on fire ants collected from monogyne mounds in Lauderdale County, Mississippi. The observed and effective number of alleles ranged from two to six and from 1.31 to 2.64, respectively. The observed and expected heterozygosity values ranged from 0.1613 to 0.7826 and from 0.1491 to 0.6242, respectively. The polymorphism information content of the microsatellites ranged from 0.1482 to 0.6208. Probability tests indicated significant deviations from the Hardy-Weinberg equilibrium at three loci. Pairwise tests did not detect linkage disequilibrium between any pair of loci.     

Culture-independent identification of gut bacteria in fourth-instar red imported fire ant, Solenopsis invicta Buren, larvae

Red imported fire ants (RIFA), Solenopsis invicta Buren, are medical, urban, and agricultural pests from South America. They are successful invaders due to their preference for disturbed habitats, high reproductive rates, and the ability to feed on a wide variety of food items (omnivorous). Fourth-instar larvae are used by the colony to digest solid food and then regurgitate it for consumption by workers and queens. Larvae are an ideal source of investigations of endosymbiotic bacteria possibly involved in nutrient distributions. Our study utilized 16S rDNA sequencing to describe the composition of the bacterial community in fourth-instar ant larvae in order to identify possible endosymbiotic bacteria present therein. The 16S rRNA gene was directly amplified from mixed-population DNA of whole fire ant larval guts and cloned into Escherichia coli. Bacterial communities from three geographically separated RIFA colonies were examined. Sequenced bacterial clones from guts were determined to be predominantly from the phylum Proteobacteria and the family Enterobacteriaceae. Our results did not detect the presence of endosymbiotic bacteria in the guts of RIFA larvae among the colonies. In addition, minimal species overlap was found when bacterial inventories were compared among colonies. Thus, bacteria coadapted with red imported fire ant larvae were not detected. Identified bacteria were not closely affiliated with endosymbiotic bacteria common in other insect species. Bacteria communities appeared to be unique to each geographical location and were determined by the foods consumed by the ants.     

我国大陆红火蚁入侵扩张趋势长期预测

红火蚁Solenopsis invicta Buren是一种严重危害农林业生产、生命健康、公共安全和生态环境的国际性重大外来入侵害虫。2004年9月中国大陆首次发现红火蚁,2021年12月该蚁已入侵了12个省区近500个县区。本研究根据中国大陆红火蚁入侵发生县区数量变化,结合潜在适生区域评价信息,建立了红火蚁入侵县区数量与入侵时间长度间关系模型,并对入侵县区数量变化趋势作出长期预测。当中国大陆红火蚁潜在入侵县区数量上限分别为1 400、1 500和1 600个时,入侵县区数量与入侵时间长度关系模型分别为N=1400/(1+e^{5.4048-0.1784T})、N=1500/(1+e^{5.4335-0.1757T})和N=1600/(1+e^{5.464-0.1734T})。基于以上模型的分析结果显示,我国红火蚁快速扩散传播从2009-2010年开始,快速扩张期可能持续26～28年,年平均新增县区41～52个/年,预测2035-2037年入侵县区数量增长速率将开始减缓,2...