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四种蝗虫前肠形态的比较研究(直翅目:斑腿蝗科) 总被引:5,自引:2,他引:3
采用体视显微摄影技术对斑腿蝗科Catantopidae3属4种(亚科)蝗虫前肠形态进行了分析,并对分布于不同地区的同一亚种进行了观察。结果表明,斑腿蝗亚科几种前肠特征同一性很高,同种不同亚种之间略有差异,相同亚种不同地区之间不存在明显差异。 相似文献
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猪瘟病毒(CSFV)囊膜表面结构糖蛋白E2(gp55)是诱导机体产生中和抗体及激发保护性免疫应答的主要抗原蛋白.E2和Erns与细胞表面受体的相互作用介导病毒对细胞的感染过程.本文采用抗CSFV(AlfortTubingen毒株)E2结构蛋白的单克隆抗体c2410和a18,淘选噬菌体展示的12肽随机肽库,对CSFV E2蛋白表位进行分析.研究发现单克隆抗体c2410和a18识别同一线性表位,定位于E2蛋白的832-837位氨基酸(SPTTLR),但二者在ELISA和免疫印迹分析中对不同表(拟)位的反应性存在差异.自杂交瘤细胞中提取总RNA,对单克隆抗体轻链和重链可变区cDNA进行序列分析.结果表明c2410和a18虽然来源于同一批次融合的杂交瘤细胞系,识别同一表位,但仍属于不同的单克隆抗体. 相似文献
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本文采用单向聚丙烯酰胺凝胶电泳法(SDS—PAGE)对十八株Frankia菌的全细胞可溶性蛋白进行了图谱分析。Frankia菌蛋白图谱不受菌龄的影响。不同接种组的菌株具有不同的蛋白图谱,同一组内的菌株也有所差异。 相似文献
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猪瘟病毒(CSFV)囊膜表面结构糖蛋白E2(gp55)是诱导机体产生中和抗体及激发保护性免疫应答的主要抗原蛋白。E2和E^ms与细胞表面受体的相互作用介导病毒对细胞的感染过程。本文采用抗CSFV(Alfort Tiibingen毒株)E2结构蛋白的单克隆抗体c2410和a18,淘选噬菌体展示的12肽随机肽库,对CSFV E2蛋白表位进行分析。研究发现:单克隆抗体c2410和a18识别同一线性表位,定位于E2蛋白的832-837位氨基酸(SPTTLR),但二者在ELISA和免疫印迹分析中对不同表(拟)位的反应性存在差异。自杂交瘤细胞中提取总RNA,对单克隆抗体轻链和重链可变区cDNA进行序列分析。结果表明:c2410和a18虽然来源于同一批次融合的杂交瘤细胞系,识别同一表位,但仍属于不同的单克隆抗体。 相似文献
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使用PEG-DS两相系统和超离心提纯的兔出血症病毒(RHDV)四个分离株病毒,再经Sepharose 4B柱层析进一步提纯后,得到较纯的病毒粒子,回收率可达70%以上。应用常规双向免疫扩散试验,交叉血凝抑制试验和酶联免疫吸附试验(ELISA)对四个不同地区分离株间的血清学关系进行了比较研究。结果表明实验中的四个分离株病毒均属同一血清型。SDS-PAGE结果表明,这四个分离株病毒均含有四条多肽,分子量为28—64KD。各株病毒多肽的分子量和各多肽在病毒粒子总蛋白中所占比例略有差异。因此四个分离株的RHDV在蛋白结构上可能存在地区差异。 相似文献
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Electrophoresis and staining of proteins from the single pollen grains of Hibiscus rosasinensis have been developed by using general ultrathin polyacrylamide gel combined with highly-sensitive silver staining technique. The result revealed that the pollen abortion could occur in different stages of pollen development. The protein patterns varied greatly in different stages of pollen development, even in the different pollen grains in the same anther at the same development stage. Some bands exhibited a disjunction by classical Mendelian ratio 1: 1, suggesting that the gene loci were heterogeneous and the proteins were related to the expression of the genes at the early stage of pollen development. 相似文献
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Enzymes catalyzing the deamidation of seed storage proteins were found in germinating seeds of kidney beans ( Phaseolus vulgaris L. cv. Moldavian) and squash ( Cucurbita pepo L. cv. Mozoleevskaya). They were partially purified and characterized. The properties of these enzymes closely resemble those of the protein deamidase in germinating wheat grains. The detection of similar protein deamidases in germinating seeds of plants belonging to phytogenetically unrelated species indicates that protein deamidases are of considerable physiological significance. 相似文献
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利用单花粉蛋白电泳技术对玉米花粉发育过程特异蛋白表达的研究 总被引:5,自引:0,他引:5
八十年代,Mulcahy(1981)与C.Ga6(1986)成功地对单个花粉粒同工酶和蛋白质进行了等电聚集电析。而国内在这方面的工作至今尚未见报道。试验利用单花粉蛋白电泳技术结合显微镜观察对不同发育时期的单个玉米花粉进行了蛋白电泳研究,结果表明玉米花粉不同发育时期所表达的蛋白质具有明显的差异:一方面随发育时期的推移某些蛋白质含量呈递减或递增趋势;另一方面不同发育时期都有特异性蛋白合成或分解。可见, 相似文献
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G. Gay C. Kerhoas C. Dumas 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1986,73(1):47-52
Summary Isoelectric focusing of proteins from single pollen grains of Cucurbita pepo L. has been developed for large scale study of pollen grain populations' heterogeneity. Forty to forty-five protein bands from one pollen grain are revealed after silver staining. Applications of this technique to pollen grain populations from different genotypes are described in this paper. Possible applications and limits of this technique are discussed with respect to plant breeding especially for the measure of gene frequencies in pollen grain populations.The work was partly supported by a grant from LIMAGRAIN and AGPM 相似文献
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Pankiw T 《Journal of economic entomology》2004,97(3):748-751
Brood pheromone modulated the foraging behavior of commercial honey bee, Apis mellifera L., colonies pollinating a 10-ha market garden of cucumber, Cucurbita pepo L., and zucchini, Cucumis saticus L., in Texas in late autumn. Six colonies were randomly selected to receive 2000 larval equivalents of brood pheromone and six received a blank control. The ratio of pollen to nonpollen foragers entering colonies was significantly greater in pheromone-treated colonies 1 h after treatment. Pheromone-treated foragers returned with pollen load weights that were significantly heavier than controls. Pollen returned by pheromone-treated foragers was 43% more likely to originate from the target crop. Number of pollen grains washed from the bodies of nonpollen foragers from pheromone-treated colonies was significantly greater than controls and the pollen was 54% more likely to originate from the target crop. Increasing the foraging stimulus environment with brood pheromone increased colony-level foraging and individual forager efforts. Brood pheromone is a promising technology for increasing the pollination activity and efficiency of commercial honey bee colonies. 相似文献
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Transmission Studies with Cucumber Green Mottle Mosaic Virus 总被引:2,自引:0,他引:2
Cucumber green mottle mosaic virus may spread in bottlegourd under field conditions through soil contaminated with infected plant debris followed by contact. No seed transmission was noticed in bottlegourd (Lagenaria siceraria) or vegetable marrow (Cucurbita pepo) although pollen grains and cotyledons from infected bottlegourd flowers or seeds, respectively, contained negliginle amounts of virus. Cucumber leaf beerles (Raphidopalpa fevicollis) are probable vectors since their regurgitated fluid and excretes contained infective virus particles. No vector fungi were found in the soil around infected bottlegourd plants. 相似文献
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Occurrence of mono- or disaccharides and polysaccharide reserves in mature pollen grains 总被引:1,自引:0,他引:1
Pollen from 13 species of gymnosperms and angiosperms was studied for soluble and insoluble carbohydrates at dispersal. Starch
reserves stored during pollen development give rise to carbohydrates at maturity. Combinations of different types of carbohydrates
in mature pollen may depend on the extent of starch hydrolysis. An inverse relationship was found between the extent of starch
hydrolysis and sucrose content. If the starch was scarcely de-polymerized, the cytoplasm had very low levels of soluble sugars
and none of the periodic acid-Schiff (PAS)-positive material as found in pollen not subject to high dehydration (Cucurbita pepo L., Zea mays L.). After total or partial starch hydrolysis, insoluble PAS-positive oligo/polysaccharides were found in the cytoplasm associated
with much soluble sugar, and the pollen grains were dehydrated at dispersal as in Typha latifolia L., Chamaerops humilis L., Trachycarpus excelsa Wendl., and other specimens. Intermediate levels of starch and soluble sugars, together with cytoplasmic PAS-positive material,
characterized species with dehydrated pollen such as Pinus halepensis Miller. Carbohydrates may be related to pollen longevity, which largely depends on the abundance of sucrose, which is known
to protect membrane integrity. The relationship between PAS-positive material and pollen viability is unclear at present.
Received: 30 July 1996 / Revision accepted: 18 December 1996 相似文献
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Pollengrainscausesomerespiratorysystemdiseasessuchasallergicasthmaandhayfever .Thesediseasesap pearespeciallyinfloweringperiodsof 相似文献
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The pollen grains in the atmosphere of Burdur have been studied for a two-year period (1996 through 1997) with a Durham sampler. A total of 11881 pollen grains belonging to 39 taxa have been identified and recorded with some unidentified ones. Of them, 5726 were identified in 1996 and 6155 in 1997. Of the total pollen grains, 76.51% were arboreal, 21.62% non-arboreal and 1.87% unidentified. The majority of the investigated pollen grains were from Pinus L., Cupressaceae, Gramineae, Quercus L., Platanus L., Chenopodiaceae/Amaranthaceae, Salix L., Cedrus L., Compositae, Juglans L. and Urticaceae respectively. The highest level of pollen concentration was in May. 相似文献