中国尾孢菌属及其近似属的研究VIII.

报道钉孢属一个新种生在梾木属Cornussp.上的梾木钉孢Passaloracorni和1个新组合生在四叶重楼Parisquadrifolia.上的重楼钉孢Passaloraparidis;尚有尾孢菌属的4个中国新记录种南蛇藤生尾孢Cercosporacelastricola.,危地马拉尾孢Cercosporaguatemalensis,大薸尾孢Cercosporapistiae,和鼠尾草生尾孢Cercosporasalviicola。为新种提供了拉丁文简介、英文描述并绘图。标本保存在中国科学院微生物研究所菌物标本馆(HMAS)。     

中国尾孢菌属及其近似属的研究VIII.(英文)

报道钉孢属一个新种：生在梾木属 Cornus sp.上的梾木钉孢 Passalora corni和1个新组合：生在四叶重楼 Paris quadrifolia.上的重楼钉孢 Passalora paridis;尚有尾孢菌属的4个中国新记录种：南蛇藤生尾孢 Cercospora celastricola., 危地马拉尾孢 Cercospora guatemalensis, 大薸尾孢 Cercospora pistiae,和 鼠尾草生尾孢 Cercospora salviicola。为新种提供了拉丁文简介、英文描述并绘图。标本保存在中国科学院微生物研究所菌物标本馆 (HMAS)。     

中国尾孢菌属及其近似属的研究XI.

本文报道15种尾孢菌,其中有3个新种和12个中国新记录种。新种是寄生在枝构Broussonetia kaepferi上的构树生尾孢Cercospora broussonetiicola, 寄生在白屈菜 Chelidonium majus 上的白屈菜尾孢Cercospora chelidonii, 寄生在律草Humulus scandens 上的律草生尾孢 Cercospora humuligena。文中为新种提供了拉丁文简介、英文描述并附图。研究的标本保存在中国科学院微生物研究所菌物标本馆 (HMAS)。     

中国尾孢菌属及其近似属的研究Ⅱ.

5种尾孢菌和2种假尾孢菌,其中有3个新种：杜若尾孢Cercosporapolliae-japonicaeY．L．Guo＆Y．Jiang,sp．nov.,日本锦带花尾孢C.weigelae-japonicaeY.L．Guo＆Y．Jiang,sp．nov．和旅覆花假尾孢PseudocercosporainulaeY．L．Guo＆Y．Jiang,sp．nov.,1个新组合：紫花假尾孢Pseudocercosporaquata（Chupp＆Greene）Y．L．Guo＆Y．Jiang,comb．nov.,和3个中国新记录;山牵牛尾孢CercosporathunbergianaYen,乌拉马尾孢CercosporauramensisChupp＆Muller和盐肤木尾孢CercosporaverniciferaeChupp＆Viegas。文中为新种提供了拉丁文简介,描述并附图,研究的标本保存在中国科学院微生物研究所菌物标本馆（HMAS）。     

Host range of Cercospora apii and C. beticola and description of C. apiicola, a novel species from celery

The genus Cercospora is one of the largest and most heterogeneous genera of hyphomycetes. Cercospora species are distributed worldwide and cause Cercospora leaf spot on most of the major plant families. Numerous species described from diverse hosts and locations are morphologically indistinguishable from C. apii and subsequently are referred to as C. apii sensu lato. The importance and ecological role that different hosts play in taxon delimitation and recognition within this complex remains unclear. It has been shown that Cercospora leaf spot on celery and sugar beet are caused respectively by C. apii and C. beticola, both of which are part of the C. apii complex. During this study we characterized a new Cercospora species, C. apiicola, which was isolated from celery in Venezuela, Korea and Greece. The phylogenetic relationship between C. apiicola and other closely related Cercospora species was studied with five different gene areas. These analyses revealed that the C. apiicola isolates cluster together in a well defined clade. Both C. apii and C. beticola sensu stricto form well defined clades and are shown to have wider host ranges and to represent distinct species.     

大豆的农艺性状与大豆灰斑病抗性关系的初步分析

对大豆灰斑病抗性水平不同的品种资源的农艺性状,包括结荚习性、株型、叶形、花色、茸毛色进行调查分析,结果表明,在灰斑病抗性较好的品种中,分枝型、圆叶、紫花、棕色茸毛的品种所占比率较大,这种现象可供今后灰斑病抗源筛选及抗灰斑病育种参考.     

Molecular identification of two strains of Cercospora rodmanii isolated from water hyacinth present in Yuriria lagoon, Guanajuato, Mexico and identification of new hosts for several other strains

Water hyacinth is a beautiful monocotyledon plant that has been dispersed all over the world by humans. The plant has been present in Mexico since 1907, and many water bodies have become infested with it since then. In 2001, we initiated a survey in Yuriria lagoon in southern Guanajuato state to isolate fungi able to biocontrol the plant. We isolated 25 morphologically distinct fungal cultures, of which two were identified as members of the genus Cercospora. Cercospora species are among the most prevalent and destructive of plant pathogens and can be found on leaves, pedicels, stems, fruits, and bracts. Only two species of Cercospora, Cercospora piaropi, and Cercospora rodmanii, have been described on water hyacinth; however, the classification of these species has been controversial. Several molecular approaches have been used for Cercospora identification, and some candidate genes have been identified for use in Cercospora species determination. Although the nrRNA genes alone do not show sufficient resolution for species determination, histone H3, translation elongation factor1-α, β-tubulin, actin, and calmodulin have been shown in previous studies to have an adequate number of nucleotide changes to allow species identification. In the present study, we used partial sequences of the histone H3, actin, and calmodulin genes to identify our two isolates as C. rodmanii. Our two strains are not specific to water hyacinth, as they are also pathogenic to beet and sugar beet. Similar host ranges were found for C. rodmanii strains isolated from Tabasco in México, Zambia, and Brazil, however, the specificity for water hyacinth persists in Cercospora piaropi Tharp and C. rodmanii Conway, the latter being the most pathogenic.     

Differential response of a sensitive and tolerant sugarbeet line to Cercospora beticola infection and UV-B radiation

Increased ultraviolet-B (UV-B, 280–320 nm) radiation, due to depletion of stratospheric ozone, is an increasing threat to living organisms. Furthermore, increased ground level temperatures as a consequence of global warming may favour development of pathogens, such as Cercospora beticola , that thrive at high temperatures. This study evaluates the effect of combined UV stress and Cercospora leaf-spot disease on young sugarbeet plants ( Beta vulgaris L . ). An inoculum consisting of twelve European isolates of C. beticola Sacc. was used in the experiments. One Cercospora -sensitive and one Cercospora -tolerant sugarbeet line were analysed from growth regimes where plants were grown either under visible radiation alone or with supplemental UV-B. Photosynthetic pigments and partial reactions of photosynthesis, including potential yield and quantum yield under illumination, non-photochemical quenching (q_NPQ) and photochemical quenching (q_P), were measured to assess plant response. The combination of Cercospora and supplemental UV-B radiation in the sensitive line resulted in a decreased photosynthetic efficiency, shown by q_NPQ and quantum yield under illumination as compared with that for either stress applied alone. The F_v/F_m was unchanged for plants subjected to UV-B radiation without infection, although the q_NPQ decreased. The Cercospora -tolerant line showed no significant differences under the different treatments. Thus, the line tolerant to Cercospora infection also proved to be tolerant to UV-B radiation alone and in combination with the infection.     

Variation in pathogenicity of isolates of Cercospora spp. attacking Heliotropium spp.

The pathogenicity of a set of 13 Cercospora samples collected in France, Turkey and Australia from four Heliotropium species has been evaluated with a detached leaf technique. All of five Euro-Asian Heliotropium species tested were susceptible to all Cercospora species isolates. However, variation in the degree of partial resistance to different isolates was observed and showed some similarity for isolates from the same region of origin. Further studies are required to clarify the taxonomy of Cercospora blight strains, but the consequences of the noted differences in pathogenicity are discussed in relation to the biological control ofH. europaeum L. in Australia.     

The photoactivated toxin cercosporin as a tool in fungal photobiology

Cercospora species are a highly successful group of fungi which pathogenize diverse species of economically important plants. Many Cercospora species produce a unique photoactivated and photoinduced polyketide toxin, cercosporin, which has been implicated as a pathogenicity factor. Illuminated cercosporin interacts with molecular oxygen to produce highly toxic singlet oxygen. Although nearly all organisms tested, including plants, mice and most fungi, are susceptible to cercosporin-mediated cell damage, Cercospora species are resistant. In general, little is known about how organisms protect themselves against singlet oxygen. Studies on how Cercospora species avoid autotoxicity are proving to be a valuable model in understanding this process in other systems. Furthermore, advances are being made in the understanding of how light regulates gene expression and cercosporin synthesis in Cercospora species. These studies are helping to elucidate mechanisms of gene regulation and light signal transduction for an environmental signal important in numerous fungal developmental processes, including secondary metabolite production.     

Genetic similarity among Cercospora apii-group species and their detection in host plant tissue by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS)

The objective of this study was to determine the genetic relatedness among the Cercospora and Pseudocercospora species closely related to Cercospora apii by using a polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) analysis of the internal transcribed spacer (ITS) region. A single PCR fragment (about 550 bp) was obtained from all Cercospora species categorized as the C. apii-group, Pseudocercospora purpurea, Pseudocercospora conyzae, and Pseudocercospora cavarae. Cercospora caricis yielded a 680 bp PCR fragment. The similarity in the PCR fragment size and RFLP profiles among the C. apii-group isolates, including Pseudocercospora purpurea, and Pseudocercospora conyzae strongly suggests that these species are conspecific. Synonymy with C. apii (lectotype) at a subspecific rank has been proposed. Amplified ITS regions of genomic DNA extracted from spinach leaves showing 12 and 233% leaf spot disease symptoms caused by Cercospora beticola yielded two PCR fragments (i.e., one from the fungus and one from the host plant) and were resolved by electrophoresis of the PCR product in 3% LMP agarose. Digestion of the total PCR product with HinfI restriction enzyme yielded RFLP profiles similar to those obtained from amplified DNA from the causative agent, C. beticola. The method described in this preliminary study offers rapid detection and diagnosis of fungal infections in plants for disease prediction and management and screening of plant materials for quarantine purposes.     

神农架丝孢菌的研究——Ⅳ.尾孢菌属,短胖孢属和色链隔孢属(英文)

作者在前两篇文章里报道了湖北省神农架产的尾孢菌属及其近似属的22个种,本文再报道这一地区的尾孢菌属、短胖孢属及色链隔孢属的4个种,其中1个是新种,即李色链隔孢(Phaeoramularia pruni Guo et Liu,sp.nov.),其模式标本收藏于中国科学院真菌标本室(HMAS)。该新种与寄生于李属(Prunus sp.)植物上的变红尾孢(Cercospora rubrotincta)接近,但后者分生孢子梗窄(2—4.5μm),不分枝,曲膝状折点少(0—1个),分生孢子向顶渐狭,不链生,隔膜多(1—5隔),因此易于区别。     

中国尾孢菌属及其近似属的研究VI.(英文)

本文报道９种尾孢菌,其中有２个新种：蟹甲草尾孢 Ｃｅｒｃｏｓｐｏｒａ ｃａｃａｌｉａｅ Ｙ． Ｌ． Ｇｕｏ　＆ Ｙ．Ｊｉａｎｇ和菜蓟尾孢Ｃｅｒｃｏｓｐｏｒａ 　ｃｙｎａｒａｅ Ｙ． Ｌ． Ｇｕｏ　＆ Ｙ． Ｊｉａｎｇ,中国新记录种有迪氏尾孢Ｃｅｒｃｏｓｐｏｒａｄｅｍｅｔｒｉｏｎｉａｎａ　 Ｇ． Ｗｉｎｔｅｒ,田菁生尾孢 Ｃｅｒｃｏｓｐｏｒａ　 ｇｌｏｔｈｉｄｉｉｃｏｌａ 　Ｔｒａｃｙ　＆ 　Ｅａｒｌｅ,甘草尾孢 Ｃｅｒｃｏｓｐｏｒａｇｌｙｃｙｒｒｈｉｚａｅ 　（Ｓａｖｕｌｅｓｃｕ　＆　Ｓａｎｄｕ）Ｃｈｕｐｐ,野桐尾孢Ｃｅｒｃｏｓｐｏｒａ　ｍａｌｌｏｔｉ　Ｅｌｌｉｓ　＆ 　Ｅｖｓｒｈ,木薯尾孢Ｃｅｒｃｏｓｐｏｒａ ｍａｎｉｈｏｂａｅ Ｖｉｅｇａｓ,补骨脂尾孢 Ｃｅｒｃｏｓｐｏｒａ ｐｓｏｒａｌｅａｅ－ｂｉｔｕｍｉｎｏｓａｅ Ｓａｖｕｌ．＆ Ｓａｎｄｕ和香豆尾孢Ｃｅｒｃｏｓｐｏｒａ ｔｒａｖｅｒｓｉａｎａ Ｓａｃｃ。文中为新种提供了拉丁文描述并附图,研究的标本保存在中国科学院微生物研究所菌物标本馆（ＨＭＡＳ）。     

中国尾孢菌属及其近似属的研究VI.

本文报道9种尾孢菌,其中有2个新种蟹甲草尾孢Cercospora cacaliae Y.L.Guo & Y.Jiang和菜蓟尾孢Cercospora cynarae Y.L.Guo & Y.Jiang,中国新记录种有迪氏尾孢Cercospora demetrioniana G.Winter,田菁生尾孢Cercospora glothidiicola Tracy & Earle,甘草尾孢Cercospora glycyrrhizae (Sǎvulescu & Sandu)Chupp,野桐尾孢Cercospora malloti Ellis & Everh.,木薯尾孢Cercospora manihobae Viégas,补骨脂尾孢Cercospora psoraleae-bituminosae Sǎvul. & Sandu和香豆尾孢Cercospora traversiana Sacc.。文中为新种提供了拉丁文描述并附图,研究的标本保存在中国科学院微生物研究所菌物标本馆(HMAS)。     

Permanent Genetic Resources added to Molecular Ecology Resources database 1 January 2009-30 April 2009

This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospora zeae-maydis; Chorthippus parallelus; Conyza canadensis; Cotesia sesamiae; Epinephelus acanthistius; Ficedula hypoleuca; Grindelia hirsutula; Guadua angustifolia; Leucadendron rubrum; Maritrema novaezealandensis; Meretrix meretrix; Nilaparvata lugens; Oxyeleotris marmoratus; Phoxinus neogaeus; Pristomyrmex punctatus; Pseudobagrus brevicorpus; Seiridium cardinale; Stenopsyche marmorata; Tetranychus evansi and Xerus inauris. These loci were cross-tested on the following species: Agalinis decemloba; Agalinis tenella; Agalinis obtusifolia; Agalinis setacea; Agalinis skinneriana; Cercospora zeina; Cercospora kikuchii; Cercospora sorghi; Mycosphaerella graminicola; Setosphaeria turcica; Magnaporthe oryzae; Cotesia flavipes; Cotesia marginiventris; Grindelia Xpaludosa; Grindelia chiloensis; Grindelia fastigiata; Grindelia lanceolata; Grindelia squarrosa; Leucadendron coniferum; Leucadendron salicifolium; Leucadendron tinctum; Leucadendron meridianum; Laodelphax striatellus; Sogatella furcifera; Phoxinus eos; Phoxinus rigidus; Phoxinus brevispinosus; Phoxinus bicolor; Tetranychus urticae; Tetranychus turkestani; Tetranychus ludeni; Tetranychus neocaledonicus; Tetranychus amicus; Amphitetranychus viennensis; Eotetranychus rubiphilus; Eotetranychus tiliarium; Oligonychus perseae; Panonychus citri; Bryobia rubrioculus; Schizonobia bundi; Petrobia harti; Xerus princeps; Spermophilus tridecemlineatus and Sciurus carolinensis.     

Development of cercospora blight epidemics and effect on the summer annual Heliotropium europaeum in the field

Heliotropium europaeum, common heliotrope, is a serious economic weed in southern Australia. Cercospora blight occurs on H. europaeum in both its native (Mediterranean) and non-native (Australian) range. The causal agents are genetically different forms of an asexual pathogen in the form-genus Cercospora in each region. Natural epidemics of cercospora blight killed weed infestations in both Australia and France. The epidemiology of the disease did not differ between the two regions once differences in rainfall had been considered. Rainfall was important for disease spread. In France, field inoculation experiments were conducted using different concentrations of conidia prepared from a monospore isolate. Cercospora blight reduced seed production 89%, but did not reduce viability. Increased inoculum concentration and inoculation of younger plants encouraged an early epidemic, but did not affect the rate of disease development once the epidemic took hold. In order to be effective at controlling this weed in Australia, these pathogens need to attack young hosts and have regular rain-splash to facilitate spread to new growth.     

尾孢菌属五个新种

本文报道尾孢菌属五个新种,即美人蕉尾孢(Cercospora cannae Bai,Liu et Guo,sp.nov.),败酱尾孢(C.patriniae Liu et Guo,sp.nov.),骆驼蓬尾孢(C.pegani Liu et Guo,sp.nov.),旌节花尾孢(C.stachyuricola Liu et Guo,sp.nov.)和安息香尾孢(C.styracicola Liuet Guo,sp.nov.)。文中对各个新种作了汉文描述和附图,并有拉丁文特征简介。模式标本收藏于中国科学院真菌标本室。     

Analysis of β-tubulin Gene Fragments from Benzimidazole-sensitive and -tolerant Cercospora beticola

Cercospora leaf spot of sugar beet, caused by the fungus Cercospora beticola, is a major foliar pathogen on sugar beet. Fungicide sprays have been used extensively to manage Cercospora leaf spot, including the benzimidazole fungicides. Resistance to benzimidazoles has been observed in isolates of C. beticola. The precise genetics of this resistance is not known in this fungus. We tested benzimidazole‐tolerant and ‐sensitive isolates and found a single mutation in the β‐tubulin gene of benzimidazole‐tolerant isolates that corresponds to a mutation known to confer benzimidazole tolerance in other ascomycetes. This mutation is predicted to cause a change from glutamic acid to alanine in the protein product. Isolates containing this mutation further show an increased sensitivity to an N‐phenylcarbamate, as would be predicted based on the mutant phenotype found in other filamentous fungi. Only a single mutation was found in isolates from different regions of the United States, isolated in different growing seasons.     

秦岭的尾孢菌及其近似属种

摘要:本文报道秦岭地区尾孢菌属及其近似属56种,其中有一个新种:秦岭假尾孢(Pseudocercospora qinlingensis Guo, sp. nov.),寄生在苦木科(Simaroubaceae)臭椿属(Ailanthus sp.)上,两个新组合:草霉色链隔孢[Phaeoramularia vexans (C. Massalongo) Guo,comb. nov.].寄生在蔷薇科(Rosaceae)草霉属(Fragaria sp.)上;篙假尾孢[Pseudocercospora dracunculi (Sarwar) Guo, comb. nov.],寄生在菊科(Compositae)牛尾蒿(Artemisia subdigitataMattf.)上和两个中国新记录种:商陆尾孢(Cercospora pircuniae Speg.),寄生在商陆科(Phytolaccaceae)商陆(Phytolacca acinosa Roxb.)上;荚蒾生尾孢(Cercospora viburnicola Ray).寄生在醉鱼草叶荚蒾(Viburnum buddleifolium C. H. Wight)上.新种(Pseudocercospora qinlingensis Guo, sp. nov.)子实体主要生于叶面,子座沿叶斑边缘发生,暗褐色至近黑色,直径40.0-75.0 (-100.0)μm,分生孢子梗紧密簇生,青黄褐色,不分枝,不呈曲膝状,无隔膜,6.5-26.0 × 3.9-6.0μm,分生孢子圆柱形,青黄褐色至中度褐色,非常弯曲,基部平截,具紧密排列地4至多个隔膜,在隔膜处缴缩,32.0-120.0 (-150.0) ×4.0-6.5 (-7.6)μm.新种与寄生在臭椿属(Ailanthus spp.)植物上的臭椿尾孢(Cercospora ailanthi P. Henn.)和臭椿生假尾孢[Pseudocercospora ailanthicola(Patwardhan) Dcighton]不同:臭椿尾孢无子座.分生孢子梗不成簇,分枝,长(50.0-200.0μm),分生孢子倒棍棒一圆柱形,色泽浅(浅青黄褐色),隔膜少(5-7隔),不缢缩,短(15.0-60.0μm);臭椿生假尾孢子实体叶背生,分生孢子梗窄((3.0-4.Spm),分生孢子倒棍棒形,隔膜少((5-8隔),不绣缩,短而窄(20.0-63.0 ×3.0-5.0μm).