A血型单克隆抗体的抗原结合部位结构多样性研究

应用杂交瘤技术,以Ａ型红细胞,Ａ１血型物质ＭＳＭ（Ａ１）和Ａ－ＲＢＣ＋ＭＳＭ（Ａ１）为免疫原,制备了一组抗人Ａ血型单克隆抗体：Ａ１２１８,Ｂ５７,ＤＥ９２３－Ｇ８,Ｄ２８６－Ｅ１２经Ｔａｋａｔｓｙ微量血细胞凝集试验证明：这组单抗仅能凝集Ａ１,Ａ２及ＡＢ型红细胞,不能凝集Ｂ,Ｏ型红细胞。采用ＥＬＩＳＡ定量抑制试验法,精确测定了它们抗原结合部位的结构,互补于Ａ活性寡糖。Ａ１２１８互补于具有双岩藻糖结构     

人ABO血型定型试剂(单克隆抗体)临床试验

通过与１１家单位协作,对中国药品生物制品检定所分发的ＡＢＯ血型单克隆抗体定型试剂进行临床考核,检测１～１２个月婴儿、５９岁以上老人、１～５９岁成人、健康献血员、癌症患者及其它疾病患者共计７０９６８例,单抗试剂检测结果与反定型结果完全相符,特别是对红细胞表面抗原位点少、亚型抗原性弱的新生儿和红细胞表面抗原成份可能发生变化的癌症患者,均呈现较好的凝集模式。     

血型检测用反向定型试剂的研制

将正常的红细胞在特定条件下用甲醛处理,使红细胞膜固定但不影响膜表面糖蛋白血型抗原的活性。采用与正向定型相同的平板凝集试验方法,４０６０份血样正向和反向定型结果完全一致。经稳定性观察９０天,处理后的红细胞与相应抗体的凝集性能未见明显改变。实验结果表明本文介绍的红细胞试剂可用于ＡＢＯ血型鉴定的反向定型试验。     

A血型单克隆抗体的抗原结合部位结构多样性研究

应用杂交瘤技术,以Ａ型红细胞,Ａ１血型物质ＭＳＭ（Ａ１）和Ａ－ＲＢＣ＋ＭＳＭ（Ａ１）为免疫原,制备了一组抗人Ａ血型单克隆抗体：Ａ１２１８,Ｂ５７,ＤＥ９２３－Ｇ８,Ｄ２８６－Ｅ１２经Ｔａｋａｔｓｙ微量血细胞凝集试验证明：这组单抗仅能凝集Ａ１,Ａ２及ＡＢ型红细胞,不能凝集Ｂ,Ｏ型红细胞．采用ＥＬＩＳＡ定量抑制试验法,精确测定了它们抗原结合部位的结构,互补于Ａ活性寡糖。Ａ１２１８互补于具有双岩藻糖结构的Ａ活性五糖（Ａ－Ｐｅｎｔａ）;Ｂ５７,ＤＥ９２３－Ｇ８互补于具有单岩藻糖结构的Ａ活性六糖（Ａ－Ｈｅｘａ）;而Ｄ２８６－Ｅ１２则互补于具有单岩藻糖的Ａ活性四糖（Ａ－Ｔｅｔｒａ）．结果表明：血凝特异性相同的抗Ａ单抗,其抗原结合部位的结构可呈现多样性。即Ａ活性寡糖的糖基组成数目和含有岩藻糖数目均可不相同,各种抑制剂对不同单抗的抑制作用强弱也不相同。     

抗rhEPO单克隆抗体的制备及初步应用

用重组人促红细胞生成素（ｒｈＥＰＯ）免疫Ｂａｌｂ／ｃ小鼠,取其脾细胞在ＰＥＣ４０００作用下与ＳＰ２／０小鼠骨髓瘤细胞融合,获得一株能分泌抗ｒｈＥＰＯ单抗隆抗体的杂交瘤细胞株２Ｆ１２,染色体数目大于１００条,间接ＥＬＩＳＡ法测定腹水和细胞培养上清效价,分别为１．６×１０＾－７和４×１０＾－４。测定抗体亚类时,则同时显示ＩｇＡ和ＩｇＧ１,其轻链为κ链;相对亲和力为５×１０％＾－１２ｍｏｌ／Ｌ。单抗２Ｆ     

中国生物化学与分子生物学报 1999年第15卷第1～6期作者索引

作者Ａｕｔｈｏｒ期－页码Ｎｏ．－Ｐａｇｅ朝仓伸司ＳＨＩＮＪＩＡＳＡＫＵＲＡ２－２６０白俊杰ＢＡＩＪｕｎｊｉｅ３－４０９白秀英ＢＡＩＸｉｕｙｉｎｇ１－１１４２－３００２－３０４毕汝昌ＢＩＲｕｃｈａｎｇ１－７９ＲｏｌａｎｄＢＬＵＭＥ４－５９３４－５９７蔡国平ＣＡＩＧｕｏｐｉｎｇ１－８３蔡建华ＣＡＩＪｉａｎｈｕａ６－１００６６－１０１２蔡以滢ＣＡＩＹｉｙｉｎｇ４－６００曹殿芳ＣＡＯＤｉａｎｆａｎｇ２－３３２曹凯鸣ＣＡＯＫａｉｍｉｎｇ４－６７１曹立环ＣＡＯＬｉｈｕａｎ１－１０５曹淑桂ＣＡＯＳｈｕｇｕｉ３…     

专一识别脱落酸甲酯的单克隆抗体的制备与应用

专一识别２－顺（Ｓ）ＡＢＡ甲酯的单克隆抗体来源于以ＡＢＡ分子中的１－ＣＯＯＨ为偶联位点合成的免疫原。它与游离态ＡＢＡ和结合态ＡＢＡ葡萄糖酯的交叉反应仅分别为１％与３．５％,而与ＡＢＡ类似物,如２－顺－黄质醛、紫黄质以及ＡＢＡ的２－反式异构体和（Ｒ）－对映体则无交叉反应。利用该抗体建立的高度灵敏和精确的ＡＢＡｍｅ酶联免疫测定法,其检测线性范围为０．０４８～１．５２ｐｍｏｌ。通过ＡＢＡｍｅＥＬＩＳＡ和ＧＡ１＋３ＥＬＩＳＡ分析可知羊蹄叶片衰老与内源ＧＡ１＋３／ＡＢＡ比值的下降有关。     

6—甲氧基—2—苯并唑啉酮（MBOA）分布不均匀是引起玉米胚芽鞘向光性运动的主

采用高效液相色谱分析等方法,对玉米胚芽鞘在单侧蓝光作用下产生的生物活性物质进行分析发现：１．在单侧蓝光作用下,胚芽鞘向不侧的生长抑制物质６－甲氧基－ ２－ 并唑啉酮（ＭＢＯＡ）含量比背光仙多１．５倍。２．向光性刺激后,胚芽鞘向光侧向背光侧生长素的含量没有出现明显的差异。３．于胚芽鞘一侧外施ＭＢＯＡ及其类似物５,－ｄｉｍｅｔｈｏｘｙ－２－ｂｅｎｚｏｘａｚｏｌｉｏｎｅ（ＤＭＢＯＡ）和２－ｃｈｌｏｒｏ－     

AgNO3对大白菜子叶芽再生的促进作用

以大白菜“０２号杂交早皇白”无菌苗的子叶为材料,用附加ＢＡ２．０ｍｇＬ＾－１、ＮＡＡ０．１～１．０ｍｇＬ％－１的ＭＳ培养基培养,能直接放导分化 出芽,最适激素比例为ＢＡ２．０ｍｇＬ＾－１、ＮＡ０．５ｍｇＬ＾－１,芽的分化率为３１．６％,在上述培养基里活加２ｍｇＬ＾－１的ＡｇＮＯ３能使芽的再生频率提高至８６．５％。     

［B1－Ala，B2－Ala］－胰岛素：受体结合特性及生物活力研究

本文报道了［Ｂ１～Ａｌａ,Ｂ２－Ａｌａ］－胰岛素与人胎盘细胞膜胰岛素受体结合的特性和体外生物活力,并与胰岛素进行比较。在３７℃和杆菌肽存在下,１２５Ｉ－［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］－胰岛素和１２５Ｉ－胰岛素与人胎盘细胞膜作用依赖于反应时间,反应６分钟到达平衡,此时,［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］－胰岛素和胰岛素与胰岛素受体的最大结合分别为每毫克膜蛋白结合６．４４ｆｍｏｌ和３．４７ｆｍｏｌ：达到平衡一半所需时间（Ｔ１／２）分别为１９秒和２５秒。用１２５Ｉ－［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］－胰岛素作为放射配体进行竞争性结合研究,从ＩＣ（５０）得［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］胰岛素的受体结合活力为胰岛素的１３９．６％。Ｓｃａｔｏｈａｒｄ分析求得;［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］－胰岛素与高亲和和低亲和结合位点的结合常数在４℃时分别为５．８８×１０８Ｌ／ｍｏｌ和７．６３×１０５Ｌ／ｍｏｌ,而胰岛素分别为４．８３×１０８Ｌ／ｍｏｌ和３．３９×１０５Ｌ／ｍｏｌ。促脂肪细胞生成脂的实验表明：［Ｂ１－Ａｌａ,Ｂ２－Ａｌａ］－胰岛素的活力为胰岛素的１３０％。     

Recent advances in the study of Kaposi's sarcoma-associated herpesvirus replication and pathogenesis

It has now been over twenty years since a novel herpesviral genome was identified in Kaposi's sarcoma biopsies. Since then, the cumulative research effort by molecular biologists, virologists, clinicians, and epidemiologists alike has led to the extensive characterization of this tumor virus, Kaposi's sarcoma-associated herpesvirus(KSHV; also known as human herpesvirus 8(HHV-8)), and its associated diseases. Here we review the current knowledge of KSHV biology and pathogenesis, with a particular emphasis on new and exciting advances in the field of epigenetics. We also discuss the development and practicality of various cell culture and animal model systems to study KSHV replication and pathogenesis.     

The structure, reproduction and taxonomy of Vidalia and Osmundaria (Rhodophyta, Rhodomelaceae)

Comprises species occurring mostly in subtidal habitats in tropical, subtropical and warm-temperate areas of the world. An analysis of the type species, V. spiralis (Sonder) Lamouroux ex J. Agardh, a species from Australia, establishes basic characters for distinguishing species in the genus. These characters are (1) branching patterns of thalli, (2) flat blades that may be spiralled on their axis, (3) width of the blade, (4) primary or secondary derivation of sterile and fertile branchlets and (5) position of sterile and fertile branchlets on the thalli. Application of the latter two characters provides an important basic method for separation of species into three major groups. Osmundaria , a genus known only in southern Australia, was studied in relation to Vidalia , and its separation from the Vidalia assemblage is not accepted. Species of Vidalia therefore are transferred to the older genus name, Osmundaria. Two new species, Osmundaria papenfussii and Osmundaria oliveae are described from Natal. Confusion in the usage of the epithet, Vidalia fimbriala Brown ex Turner has been clarified, and Vidalia gregaria Falkenberg, described as an epiphyte on Osmundaria pro/ifera Lamouroux, is revealed to be young branches of the host, Osmundaria prolifera.     

New or rare chromosome counts in Artemisia L. (Asteraceae, Anthemideae) and related genera from Kazakhstan

Fifteen chromosome counts of six Artemisia taxa and one species of each of the genera Brachanthemum, Hippolytia, Kaschgaria, Lepidolopsis and Turaniphytum are reported from Kazakhstan. Three of them are new reports, two are not consistent with previous counts and the remainder are confirmations of very scarce (one to four) earlier records. All the populations studied have the same basic chromosome number, x = 9, with ploidy levels ranging from 2x to 6x. Some correlations between ploidy level, morphological characters and distribution are noted.     

肝癌中HBV和HCV基因和抗原的分布及意义

采用原位分子杂交方法检测HCV RNA及HBV X基因;采用免疫组织化学方法研究HCV核心抗原,非结构区C33c抗原及HBxAg在肝细胞肝癌中的定位及分布.结果表明(1)HCV RNA、HBV X基因在肝细胞肝癌组织检出率分别为40%(55/136)和82%(112/136).HCV RNA定位于癌细胞的胞浆内,阳性细胞呈散在、灶状及弥漫分布三种形式;HBV X基因在肝癌细胞中的分布呈胞浆型、核型及核浆型,阳性细胞也呈上述三种分布形式;(2)HCV C33c抗原、核心抗原在肝细胞肝癌中的阳性率为81%(133/164)及86%(141/164).C33c抗原定位于癌细胞及肝细胞的胞浆内;核心抗原既定位于癌细胞核中,又可定位于胞浆中.C33c抗原阳性细胞以灶状分布为主;而核心抗原阳性细     

Selection with two alleles and complete dominance

For a plant selection model with frequency-independent viabilities, fertilities and selfing rates, it is shown that apart from global fixation, for certain parameter combinations a protected polymorphism and facultative fixation (either allele may become fixed according to initial frequencies) may both occur. Facultative fixation requires different selling rates for the dominant and recessive type. Protection of the polymorphism requires resource allocation for male and female function. In this connection the problem of purely genetically caused population extinction is discussed.
For general frequency dependence and regular segregation, the chances for establishment of a completely recessive gene are compared to those of a completely dominant gene. It is proven that the process of establishment of the recessive gene, despite a fitness advantage, may be considerably endangered by drift effects if random mating prevails. The recessive gene may reach the same effectivity in establishment as a dominant gene, only if the recessive homozygote mates exclusively with its own type during the period of establishment.