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1.
兴奋性氨基酸加强高原低氧大鼠下丘脑前生长抑素原mRNA的表达 总被引:5,自引:0,他引:5
目的和方法:应用大鼠高原低氧模型及原位杂交技术和氨基酸测定法,研究下丘脑前生长抑素原(PPS)mRNA表达和谷氨酸(Glu)、天门冬氨酸(Asp)含量的变化。结果:高原低氧组大鼠下丘脑Glu和Asp的含量明显增多,室周核、室旁核、弓状核PPS-mRNA阳性神经元数目显著增加;而NMDA受体拮抗抗剂氯铵酮,虽然对Glu和Asp含量无明显影响,但可使高原低氧大鼠下丘脑PPS-mRNA阳性神经元数目减少 相似文献
2.
全蝎抗癫痫发作敏感性的阿片肽机制 总被引:9,自引:0,他引:9
红藻氨酸(KainicAcid,KA)癫痫模型,探讨全蝎抗癫痫发作敏感性长期增强的阿片肽机制。本实验选用SD大鼠,随机分为两组,分别给予生理盐水(NS)和全蝎粗提液灌胃10天,10天后两组均分别颈部皮下注射NS和惊厥剂量(10mg/kg)的KA,再分别继续给予NS和全蝎粗提液灌胃10天后,用阈下剂量(5mg/kg)的KA检测癫痫敏感性;用Fos免疫反应活性检测海马结构中神经元的兴奋性;用原位杂交技术检测海马脑啡肽原(PENK)mRNA的动态变化过程。结果显示:实验对照组大鼠癫痫行为敏感性明显增强,脑内癫痫敏感性相关脑区海马齿状回颗粒细胞(DGCs)c-Fos免疫反应阳性细胞数目明显增加,同时海马内具有致癫痫作用的脑啡肽原(PENK)mRNA表达也明显增加;而实验组动物未见上述改变。本工作证实中药全蝎有明显降低海马神经元兴奋性及抗癫痫发作敏感性形成的作用,并提示这很可能与其抑制PENKmRNA表达增加有关 相似文献
3.
为探讨紫外线对晶状体的损伤机制,用RT-PCR方法(reversetranscription-polymerasechainreaction,反转录聚合酶链反应),研究经紫外线照射后大鼠晶状体抗氧化相关酶,包括铜锌-超氧化物歧化酶(copper-zinc-superoxidedismutase,Cu-Zn-SOD),谷胱甘肽过氧化物酶(glu-tathioneperoxidase,GSH-Px)和过氧化氢酶(catalase,CAT)等mRNA的表达.结果显示,短时间的照射(2~5min),抗氧化相关酶的mRNA表达水平有增高表现,随后其mRNA表达水平开始下降,15min时抗氧化相关酶mRNA的表达下降更为明显,与对照组相比有非常显著性差异(P<0.001).照射后24h,抗氧化相关酶的mRNA表达有不同程度的恢复;照射后48h,其mRNA表达水平基本恢复,与对照组相比没有显著性差异.从而从基因水平上初步探讨了紫外线的氧化损伤机制 相似文献
4.
用6-OHDA损毁大鼠一侧黑质—纹状体通路可引起PD模型鼠脑纹状体内多巴胺匿乏.同时,亦可导致脑啡肽mRNA过度表达。我们用地高辛标记的cRNA探针对纹状体内脑啡肽mRNA含量进行了斑点杂交定量研究,发现损伤侧脑啡肽mRNA含量较健侧增高80%,胎中脑移植到失神经支配的纹状体内,脑啡肽mRNA过度表达得以矫正至正常水平,说明胎多巴胺能神经元脑内移植能够调节脑啡肽基因表达,提供了移植物能与宿主发生神经整合、建立突触联系的间接证据。 相似文献
5.
青霉素诱发癫痫及耳穴电针后大鼠前脑生长抑素mRNA的变化 总被引:1,自引:0,他引:1
应用地高辛标记的生长抑素(SS)RNA探针原位杂交法观察大鼠在青霉素诱发的癫痫及耳穴电针抑制癫痫发作后有关的脑区中SSmRNA含量的变化。结果显示,青霉素致痫后24小时梨状皮质、额叶皮质、扣带回、隔外侧核、杏仁基底核海马CA1-CA4区和齿状回颗粒细胞层、多形层等脑区SSmRNA的表达显著增加,与正常对照组比较P<005。耳穴电针抑痫(80100HZ,6mA)后额叶皮质、杏仁基底核、海马齿状回、前梨状皮质SSmRNA的表达明显减少,P<005。提示前脑结核中的生长抑素与青霉素致痫和耳针抑痫有关。 相似文献
6.
促肾上腺皮质激素对慢痛大鼠脊髓生长抑素、c-fos表达及痛反应的影响 总被引:3,自引:0,他引:3
本研究应用免疫组化、原位杂交和痛级均数测定法,探讨鞘内注射促肾上腺皮质激素(ACTH)对甲醛引起大鼠脊髓内生长抑素(Som)、cfos表达及痛反应的影响。结果表明,足底注射甲醛可使大鼠脊髓内cfos样免疫反应(FLI)、Som样免疫反应(SomLI)、FLI/SomLI及前Som原mRNA(PPSmRNA)神经元数目显著增多以及痛级均数(PIR)显著升高。而鞘内注射ACTH可显著抑制甲醛引起的大鼠脊髓内FLI、SomLI、FLI/SomLI及PPSmRNA增多和PIR升高效应。鞘内预先注射赛庚啶可阻断ACTH的抑制效应,而荷包牡丹碱、纳洛酮则无影响。结果提示,5羟色胺受体可能参与ACTH抑制甲醛引起的痛反应。 相似文献
7.
运动对高血压性肥大心脏心肌肌球蛋白重链基因表达的影响 总被引:7,自引:0,他引:7
研究运动逆转高血压肥大心脏心肌肌球蛋白重链(myosin heavy chain ,MHC)异型改变的分子机制。方法:采用Northern 分子杂交方法对自发性高血压大鼠(spontaneously hypertensive rats,SHR) 游泳运动10 周后心肌MHC基因表达进行比较研究。结果:游泳SHR收缩压和舒张压分别比安静SHR 降低22 % 和25% (P< 0.01) 。左心室重/体重(LVW/BW)比值两组间无明显差异(P> 0.05)。游泳SHR 心肌αMHCmRNA表达比安静SHR增强17% ,βMHCmRNA 表达降低26 % ,α/βMHC基因表达比值提高59 % 。结论:运动逆转高血压肥大心脏心肌MHC同型异构体转变的调控机制可发生在基因转录水平上 相似文献
8.
缺氧对大鼠肺组织肺血管sis原癌基因和PDGF-BB蛋白表达的影响 总被引:2,自引:0,他引:2
为了探讨血小板生长因子BB(PDGF-BB)在缺氧性肺血管结构重塑中作用,通过建立大鼠缺氧性肺血管重塑动物模型,运用原位杂交技术和免疫组织化学染色技术检测不同缺氧时期大鼠肺组织中原癌基因sismRNA和PDGF-BB蛋白表达。结果:(1)随着缺氧时间的延长,肺动脉壁肌层增厚,管腔变窄;(2)正常对照组肺组织及肺血管壁c-sismRNA和PDGF-BB蛋白质均极少表达(+);(3)缺氧3天后,两者表达均增多();(4)缺氧7天后其表达达高峰并持续至14天(~);(5)缺氧21天后,两者表达均降低,但仍高于正常对照组()。提示:缺氧可以刺激大鼠肺组织sismRNA和PDGF-BB蛋白表达,其在缺氧性肺血管结构重塑形成中可能具有重要作用。 相似文献
9.
培养大鼠主动脉血管平滑肌细胞产生巨噬细胞集落刺激因子及其受体的表达 总被引:1,自引:0,他引:1
本研究用培养大鼠主动脉血管平滑肌细胞(VSMCs),结果如下:(1)用生物活性检测法发现VSMCs无血清条件培养液可刺激巨噬细胞集落形成,其作用能被抗巨噬细胞集落刺激因子(MCSF)抗体抑制;(2)用免疫细胞化学技术证明VSMCs存在MCSF受体;(3)用Northern blot技术证明VSMCs有MCSF及其受本的mRNA表达,血清刺激使两者表达明显增强。本研究首次报道了培养大鼠主动脉VSMC 相似文献
10.
氯氨酮和L-NAME抑制模拟高原低氧大鼠下丘脑NOS和生长抑素mRNA的表达 总被引:3,自引:0,他引:3
用高原低氧模型及原位杂交、NADPH-d组织化学法,探讨氯氨酮和L-NAME对急性高原低氧大鼠下丘脑一氧化氮合酶(NOS)和生长抑素mRNA(SS mRNA)表达的影响。结果表明,急性高原低氧引起下丘脑NOS和SS mRNA过度表达,如先用NMDA受体拮抗剂氯氨酮和NOS抑制剂L-NAME预处理,NOS和SS mRNA的表达均明显被抑制。结果提示,NMDA受体参与了急生高原低氧引起的下丘脑NOS和 相似文献
11.
Gary J. Fosmire Stephen J. Focht Gerald E. McClearn 《Biological trace element research》1993,37(2-3):115-121
Aluminum is a known neurotoxin and has been suggested to play a role in the development of Senile Dementia of the Alzheimer's
Type. The relationship between aluminum exposure and senile dementia cannot be a simple one, however, as not all exposure
results in neurotoxic manifestations. To determine if there are genetic differences in susceptibility to moderate aluminum
exposure, 16 mice of five inbred strains were divided into two groups. The control group was fed a purified diet containing
all known requirements for mice; the experimental group was fed the same diet supplemented with 260 mg Al/kg diet for 28 d.
Analysis of brains, livers, and tibias for aluminum concentrations revealed strain differences in response to dietary treatment.
The most notable results occurred in the DBA/2 and C3H/2 strains, with brain aluminum levels higher in the experimental groups.
In contrast, A/J, BALB/c, and C57BL/6 strains showed no differences in brain aluminum in response to dietary treatment. These
findings suggest that there are genetic differences in the permeability of the blood brain barrier and lend support to the
hypothesis that variability in aluminum toxicity may be, in part, genetically determined. 相似文献
12.
黄颡鱼饲料中添加谷胱甘肽降低藻毒素毒性作用的研究 总被引:1,自引:0,他引:1
通过60d的生长实验研究饲料中不同含量的谷胱甘肽(GSH)对黄颡鱼(Pelteobagrus fulvidraco Richardson)摄食含蓝藻粉饲料后的毒性保护作用。共配制6种近似等氮等能(粗蛋白45%,总能19.5kJ/g)的实验饲料。以不添加蓝藻粉、不添加GSH的饲料组作为正对照,其他5种饲料均添加7.5%的藻粉,微囊藻毒素(Microc ystins,MCs)含量约为58μg/g,GSH添加量分别为:0(负对照)、200、500、800和1400mg/kg。实验结果表明,第1至第30天时,负对照组与正对照组实验鱼的摄食率无显著性差异(P>0.05)。第31至第60天时,负对照组实验鱼的摄食率显著高于正对照和其他GSH添加组(P0.05),但各GSH添加组肾脏中GSH显著低于正对照而高于负对照(P<0.05)。200、500和800mg/kg GSH添加组肝脏和肌肉中丙二醛(MDA)含量显著低于负对照(P<0.05)。在整个实验期间,随着饲料中GSH含量的增加,肝脏、肾脏、全肠和肌肉中微囊藻毒素含量先显著下降,当GSH添加量为500和800mg/kg时达到稳定,直至GSH添加量为1400mg/kg时显著上升。根据对黄颡鱼增重,肝脏和肌肉中MDA的含量以及组织中毒素的分析,在饲料中添加500-800mg/kg GSH可以有效缓解黄颡鱼饲料中微囊藻毒素的毒性。
相似文献
13.
A breeding trial was conducted to determine if a semen extender containing polymixin-B sulfate would improve the fertility of a stallion with seminal vesiculitis due to Pseudomonas aeruginosa . Twenty-three mares were bred to the stallion by one of three methods: artificial insemination with raw semen (Group 1, n = 10), artificial insemination with semen mixed 1:1 with a nonfat dry skim milk/glucose extender containing 1000 units/ml polymixin-B sulfate (Group 2, n = 9), or natural service immediately following infusion of the uterus with 100 ml of the same extender (Group 3, n = 4). Artificial breedings contained a minimum insemination dose of 500 x 10(6) progressively motile spermatozoa. All mares were bred every other day while in estrus. Pregnancy status was determined by transrectal ultrasound examination 15 d after the last breeding. First-cycle pregnancy rate for Group 2 mares (78%) was greater (P < 0.01) than for Group 1 mares (10%). There was a tendency (P = 0.10) for the pregnancy rate of Group 3 mares (50%) to be greater than Group 1 mares. The use of a semen extender containing polymixin-B sulfate improved the fertility of this stallion. 相似文献
14.
《Free radical research》2013,47(5):299-309
Copper, zinc superoxide dismutase (Cu, ZnSOD) and manganese superoxide dismutase (MnSOD) activities were measured in mouse large intestinal mucosa during dimethylhydrazine (DMH) carcinogenesis. Mice were divided into five groups. Group A was subcutaneously injected with DMH (20mg/kg) weekly and fed with a diet containing 0.2% cholic acid (C) and 0.8% indole (I). Group B was injected with DMH and given indole feeding. Group C was treated with DMH injection and cholic acid feeding. Group D was given DMH injection alone. Group E was an age-matched control group given 0.9% NaCl injection. The experiment last 21 weeks. The Cu, ZnSOD activity of intestinal mucosa in group A animals began to increase significantly at the 7th week of the experiment. In groups B, C and D, however, this enzyme was not elevated statistically until the 16th week, and then each of these groups kept an increased Cu, ZnSOD level the rest of the experimental period. MnSOD activity was elevated statistically in group C animals at the 7th week. The enzyme activity in group A and D animals increased at the 9th week, but the enzyme activity did not increase statistically until the 11th week in group B. After the 16th week of the experiment the increased activity of MnSOD in all experimental groups returned to the level of the control group. Large intestinal cancer tissues had increased Cu, ZnSOD activity and decreased MnSOD activity. 相似文献
15.
Copper, zinc superoxide dismutase (Cu, ZnSOD) and manganese superoxide dismutase (MnSOD) activities were measured in mouse large intestinal mucosa during dimethylhydrazine (DMH) carcinogenesis. Mice were divided into five groups. Group A was subcutaneously injected with DMH (20mg/kg) weekly and fed with a diet containing 0.2% cholic acid (C) and 0.8% indole (I). Group B was injected with DMH and given indole feeding. Group C was treated with DMH injection and cholic acid feeding. Group D was given DMH injection alone. Group E was an age-matched control group given 0.9% NaCl injection. The experiment last 21 weeks. The Cu, ZnSOD activity of intestinal mucosa in group A animals began to increase significantly at the 7th week of the experiment. In groups B, C and D, however, this enzyme was not elevated statistically until the 16th week, and then each of these groups kept an increased Cu, ZnSOD level the rest of the experimental period. MnSOD activity was elevated statistically in group C animals at the 7th week. The enzyme activity in group A and D animals increased at the 9th week, but the enzyme activity did not increase statistically until the 11th week in group B. After the 16th week of the experiment the increased activity of MnSOD in all experimental groups returned to the level of the control group. Large intestinal cancer tissues had increased Cu, ZnSOD activity and decreased MnSOD activity. 相似文献
16.
目的:探究微囊藻毒素-LR对小鼠肝细胞线粒体功能的影响。方法:采用BALB/c小鼠作为模型动物,随机分为3组:A组,空白对照组,正常饮用水;B组,添加5g门L微囊藻毒素.LR的饮用水;C组,添加30gm微囊藻毒素-LR的饮用水。分组喂养3个月,分离小鼠肝脏、提取线粒体,采用线粒体荧光探针JC-1测定线粒体膜电位(MMP),qRT-PCR检测自噬相关基因Beclinl和Lc3α的转录水平,WesternBlot检测细胞色素c的释放,电镜观察线粒体的形态和内部结构。结果:微囊藻毒素-LR处理组的小鼠肝细胞线粒体膜电位明显下降,自噬相关基因Lc3α的转录水平上升,细胞色素C由线粒体释放到胞浆,电镜观察线粒体形态异常、内部结构被破坏。结论:微囊藻毒素-LR对小鼠肝细胞线粒体有较强的毒性作用,并引发线粒体自噬。 相似文献
17.
饵料对鱼类生长发育和繁殖具有重要影响。为了筛选稀有鲫(Gobiocypris rarus)幼鱼和成鱼阶段最适投喂方式, 实验将出膜5周末(日龄为35 day after hatching)的幼鱼随机分为5个组: A组投喂丰年虫; B组每周前6d (days)投喂丰年虫, 后1d投喂商业化微颗粒S3饲料; C组每周前3.5d投喂丰年虫, 后3.5d投喂饲料; D组每周前1d投喂丰年虫, 后6d投喂饲料; E组一直投喂饲料; 各组均采用饱食投喂策略。每2周统计生长、存活指标, 直至第21周(147 dah), 在17周(119 dah)取材用于观察性腺发育程度。在产卵后统计各组产卵量、孵化率和子代畸形率。结果显示: (1)E组存活率和特定生长率显著低于其他组(P<0.05); (2)从产卵量、孵化率和子代畸形率上看, B组产卵量显著高于其他组(P<0.05); (3)从性腺组织学上看, 不同投喂方法对精巢的成熟度无显著影响, 但投喂过丰年虫的稀有鲫卵巢发育成熟度显著优于E组。研究结果提示:适量加入丰年虫比单一投喂活饵或饲料更有利于稀有鲫的生长和繁殖。建议在标准化养殖过程中, 幼鱼和成鱼期的稀有鲫采取丰年虫与饲料投喂频次比值为6﹕1的方式最佳。 相似文献
18.
实验采用刚断乳的幼鼠20 只, 分为两组喂养, 每组10 只, 对照组和普通饲料喂养(简称A 组),另一组用奶粉喂养(简称B组)。分别喂养20天后, 对其肝组织化学进行了分组定性对比观察。结果显示:A 组肝细胞糖原PAS反应, SDH、ChE活性高于B组, 分别为强阳性(), 而B组LDH, AKP活性比A组强, 分别为强阳性及最强阳性 (, )。 相似文献
19.
The effects of different modes of inulin supplementation on caecal fermentation were evaluated in rats. Groups S and IN were fed diets containing 5% of sucrose or inulin, respectively, for the whole experimental period of 40 days. Group IN/S was fed IN and S diets, whereas group S/IN was fed S and IN diets, in the first and the second 20-day period, respectively. Groups IN(up) and IN(down) were fed diets in which the content of inulin increased from 1-5% and decreased from 5-1%, every 8 days, respectively. The common effects of inulin on caecal fermentation, i.e. enlargement of tissue, acidification of digesta, a decrease in activities of potentially harmful bacterial enzymes (beta-glucuronidase and beta-glucosidase), and an increase in the total volatile fatty acids concentration and pool, were especially observed in the IN, S/IN and IN(up) groups. The results suggested that the intensity of caecal fermentation is increased when inulin is present at a relatively high dietary level and that these changes are easily reversible after inulin withdrawal from feed. 相似文献
20.
30只雌性SD大鼠分为五组,即A组(阴性对照组),B组(孕三烯酮阳性对照组1mg/kg),C组(米非司酮实验组,Cl12mg/kg,C26mg/kg,C33mg/kg)。用组织化学方法观察米非司酮对子宫内膜一氧化氮合成酶(NOS)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、酸性磷酸酶(ACP)、硷性磷酸酶(ALP)活性和糖原含量的影响。实验结果显示:NOS,SDH和ALP活性较阴性对照组弱,LDH和ACP活性较阴性对照组强,糖原含量略低于阴性对照组。 相似文献