共查询到20条相似文献,搜索用时 31 毫秒
1.
M Schindl E Wallraff B Deubzer W Witke G Gerisch E Sackmann 《Biophysical journal》1995,68(3):1177-1190
Reflection interference contrast microscopy combined with digital image processing was applied to study the motion of Dictyostelium discoideum cells in their pre-aggregative state on substrata of different adhesiveness (glass, albumin-covered glass, and freshly cleaved mica). The temporal variations of the size and shape of the cell/substratum contact area and the time course of advancement of pseudopods protruding in contact with the substratum were analyzed. The major goal was to study differences between the locomotion of wild-type cells and strains of triple mutants deficient in two F-actin cross-linking proteins (alpha-actinin and the 120-kDa gelation factor) and one F-actin fragmenting protein (severin). The size of contact area, AC, of both wild-type and mutant cells fluctuates between minimum and maximum values on the order of minutes, pointing toward an intrinsic switching mechanism associated with the mechanochemical control system. The fluctuation amplitudes are much larger on freshly cleaved mica than on glass. Wild-type and mutant cells exhibit remarkable differences on mica but not on glass. These differences comprise the population median of AC and alterations in pseudopod protrusion. AC is smaller by a factor of two or more for all mutants. Pseudopods protrude slower and shorter in the mutants. It is concluded that cell shape and pseudopods are destabilized by defects in the actin-skeleton, which can be overcompensated by strongly adhesive substrata. Several features of amoeboid cell locomotion on substrata can be understood on the basis of the minimum bending energy concept of soft adhering shells and by assuming that adhesion induces local alterations of the composite membrane consisting of the protein/lipid bilayer on the cell surface and the underlying actin-cortex. 相似文献
2.
Impaired neutrophil locomotion associated with hyperadhesiveness in a patient with Chédiak-Higashi syndrome 总被引:1,自引:0,他引:1
Neutrophils from a patient with Chédiak-Higashi (CH) syndrome exhibited defective directional locomotion in a gradient of activated plasma. Further analysis of the nature of this defect showed that CH neutrophils could respond normally to stimulation with f-Met-Leu-Phe, by increasing both their motility and polarization, provided the cells were kept in suspension. Contact with the substratum resulted in the loss of both motility and polarity in the majority of cells. CH neutrophils, in contrast to normal cells, did not respond chemokinetically to f-Met-Leu-Phe. Unstimulated random locomotion of CH neutrophils was also depressed, and this correlated with increased spreading on the substratum. Our results indicate that motility, locomotion and polarisation of CH neutrophils on the substratum are depressed because of excessive adhesion. 相似文献
3.
L P Bignold 《Cell biology international reports》1986,10(7):535-543
Crawling-like movements, motility and adhesiveness of polymorphonuclear leukocytes (PMN) in various concentrations of autologous plasma were assessed using microporous cellulose acetate membranes. In 0% plasma, crawling-like movements and motility were low, while adhesiveness was high. In 10% plasma, crawling-like movements remained low, motility was high and adhesiveness fell to a minimum. In 30%, 60% and 100% plasma, crawling-like movements progressively increased, motility rose (at 30%) and then fell slightly (at 100%) while adhesiveness did not change. The results indicate that crawling-like movements of PMN suspended in plasma are not a good indicator of the motility of these cells in cellulose acetate membrane. 相似文献
4.
The role of sulfhydryl groups in human neutrophil adhesion, movement and particle ingestion 总被引:1,自引:0,他引:1
Recent studies have suggested that ectosulfhydryl groups may play a role in cell contact phenomena. We have studied the possible role of ecto- and endosulfhydryl groups in the morphology, adhesiveness, random and directed (chemotaxis) motility and phagocytosis of human polymorphonuclear neutrophils. The rapidly penetrating sulfhydryl binding reagents HgCl2 and NEM inhibited adhesiveness, motility and phagocytosis when studied at > 0.1 mM in plasma or > 0.01 mM in buffer. The difference in inhibitory concentration was shown to be due to the difference in albumin content of the two media. D-cysteine prevented the effect of HgCl2 and NEM on cell morphology, adhesiveness, motility and phagocytosis indicating that their effects were on cell sulfhydryl groups. PCMBS, a very slowly penetrating organic mercurial, had no effect on neutrophil morphology, adhesiveness, motility or phagocytosis. However, PCMBS inhibited platelet aggregation, assuring its potency. These studies indicate that ectosulfhydryl groups are either not present or not participants in the maintainence of structure and functions of human neutrophilic granulocytes. 相似文献
5.
Effects of anaesthetics on membrane mobility and locomotor responses of human neutrophils 总被引:1,自引:0,他引:1
D. Hulse J.R. Kusel N.G. O'Donnell P.C. Wilkinson 《FEMS immunology and medical microbiology》1994,8(3):241-248
Abstract The morphological response of neutrophils to chemotactic factors is characterized by an immediate change (in seconds) from a spherical to an irregular shape. Within two or three minutes, the cells assume the head-tail polarity typical of locomotor cells. In this study the effects of the anaesthetic drugs, propofol and thiopentone, on the time-sequence of the morphological response of human neutrophils to the chemotactic peptide fMet-Leu-Phe were examined. At concentrations seen in the plasma during anaesthesia, both drugs inhibited both the rate and degree of the neutrophil chemotactic response. The effect of propofol was not attributable to its lipid vehicle, as 10% intralipid alone had no effect on neutrophil polarization. Plasma membrane reorganization occurs during polarization of neutrophils, resulting in morphological and functional changes which prepare the cells for chemotaxis and phagocytosis. Fluorescence recovery after photobleaching (FRAP) was used to investigate effects of the anaesthetics on membrane lipid behaviour. With a lipid probe, the proportion of mobile lipid in neutrophils exposed to propofol or thiopentone was reduced. There was a less significant reduction with intralipid which also caused reduction in velocity of lateral diffusion of the probe. These findings suggest that the inhibitory effects of anaesthetics on neutrophil locomotion are related to reductions in fluid mobility of the plasma membranes of anaesthetic-treated cells. 相似文献
6.
John A. Trotter 《Experimental cell research》1981,132(2):235-248
The cytoskeleton of murine peritoneal macrophages has been examined by a combination of morphological techniques, including phase-contrast light microscopy, scanning electron microscopy (SEM), and several transmission electron microscopic (TEM) methods. The cytoskeleton of cells spreading on glass, Formvar-carbon, and polystyrene substrata was exposed by brief extraction with non-ionic detergent, and stabilized by exposure to heavy meromyosin, myosin subfragment-1 or tropomyosin. In the spreading lamellae and lamellipodia the cytoskeleton is principally composed of filamentous actin, which appears as dense foci, interconnected by radiating filaments and filament bundles. The actin of the foci, as well as individual actin filaments, are connected to the substratum by transmembrane linkages which appear as filaments that pass through the plane of the (extracted) plasma membrane. Thus, the results of this study indicate that the adhesion of macrophages to substrata for the purposes of spreading and motility may be a function of transmembrane elements which link actin to substrata. Further, the formation of actin foci may serve to stiffen and stabilize the cytoskeleton, conditioning it to function in cell adhesion, spreading and locomotion. 相似文献
7.
J. Kołodziejczyk W. Kłopocka A. Łopatowska L. Grebecka A. Grebecki 《Protoplasma》1995,189(3-4):180-186
Summary Floating heterotactic cells ofAmoeba proteus were sedimented on untreated glass surfaces and on modified substrata, differing in their wettability and surface potential. About 95% of the amoebae readhere to the glass within 12 min and recover locomotive (polytactic) morphology within 13 min. The rate of locomotion resumption does not change significantly on styrene/methyl methacrylate co-polymers with contrasting hydrophilic sulfonic group surface densities. Almost all amoebae readhere within 3 min to the positively charged surface of polylysine-coated glass, but locomotive shape is only reassumed after 20 min by 95% of them. The polytactic cells are marked flattened on polylysine and move 2 1/2 times more slowly than on the glass. Floating amoebae never readhere to negatively charged gelatin gel; up to 25% become polytactic after 20 min, but they never resume locomotion. Indifference of amoebae to substratum wettability, and their prompt reaction to the positively or negatively charged surfaces, are discussed. The polylysine and gelatin gel substrata seem suitable for the study of adhesion dependent motor functions in amoebae. 相似文献
8.
A novel method for the comparative analysis of cell motility by direct viewing was developed and used in a preliminary study of sarcoma cells. Three cell lines from the RPS family of sarcomas in inbred LEW/CUB rats which differ in the incidence of spontaneous metastasis were studied. A system of four different culture conditions, designed to mimic the stress within the tumour, was created by changing and combining two variables: the pH of the medium (with 2% calf serum only), which was either physiological at 7.4 or 6.6; and the adhesiveness of the culture substratum, which was either at a standard level or decreased. It was found that changing the pH from 7.4 to 6.6 in a single step slowed the cells down, and also changed the way in which they moved, from «walking« in random directions to moving in a more directional way. Making the culture surface less adhesive sped up cell locomotion. Decreasing the adhesiveness of the culture substratum and the pH simultaneously stimulated the migration of highly metastasizing cells preferentially. Thus we have found a way to distinguish between highly metastasizing A297Nb sarcoma cells and poorly metastasizing T15 and non-metastasizing K2 sarcoma cells, a distinction that could not be made by a simple examination of the cells. It is concluded that a comparison of cell motility by directly viewing the cells under different conditions may be useful when investigating the in vitro motility properties of malignant cells. 相似文献
9.
Albert Harris 《Experimental cell research》1973,77(1-2)
The locomotory behavior of tissue cells cultured on various artificial substrata was studied by time-lapse cinemicrography. Cells were able to spread more completely on certain more wettable substrata and to accumulate preferentially on these substrata according to a consistent hierarchy of cell-substratum affinity, which was the same for all cell types. Cell responses to variations in substrata suggest that substratum adhesiveness is the determining factor, but that cells accumulate on more adhesive substrata as the result of unequal competition between several actively locomotory ruffled lamellae around their margin. The increased overlapping between cells cultured on less adhesive substrata was found to be attributable to factors other than a decrease of contact inhibition of locomotion. 相似文献
10.
I I Dolgushin E L Khasman E I Romashevskaia 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1979,(6):23-27
The degree of immune response to corpuscular antigen and macrophage reaction within 24 hours after thermal injury were examined in a comparative study. The number of antibody-producing cells, antigen engulfment and elimination by the cells of peritoneal exudate, their adhesiveness to glass, and cathepsin activity in the cells of peritoneal exudate and the spleen were determined. Mice subjected to thermal burns showed suppressed immune response and decreased functional activity of the cells of the monocytic phagocyte system within the first 24 hours, which was manifested by a decrease in the adhesiveness of these cells to glass, and their ability to engulf and destroy xenogenic erythrocytes. 相似文献
11.
D Càllari G Blandino V Saccone C D'Amico A Billitteri 《Bollettino della Società italiana di biologia sperimentale》1992,68(12):789-794
Since cell adhesiveness is very important in the metastatic process and because both hyperthermia and treatment with Retinol can modify the fluidity of the lipid components of the plasma membrane (and therefore its receptor distribution), we investigated if a hyperthermic treatment (at 42 degrees C or 44 degrees C, for one hour) of HTC hepatoma cells, preceded or followed by treatment with 5 microM Retinol, could alter cell adhesiveness to Laminin or to Fibronectin-coated substrata. Hepatoma cells, after such treatments, were collected and processed by Auerbach's method. In the control cells thermal treatment alone caused a decrease of adhesiveness to Laminin but no change in that to Fibronectin. When treatment with Retinol was carried out before hyperthermia, the cells showed an increased adhesiveness to Laminin and a decreased adhesiveness to Fibronectin. Instead, when treatment with Retinol was performed in cells previously thermo-selected, a decrease of adhesiveness to both tested ligands was observed. 相似文献
12.
Locomotion of sponges and its physical mechanism 总被引:6,自引:0,他引:6
Active locomotion by individual marine and freshwater sponges across glass, plastic and rubber substrata has been studied in relation to the behavior of the sponges' component cells. Sequential tracing of sponge outlines on aquarium walls shows that sponges can crawl up to 160 microns/hr (4 mm/day). Time-lapse cinemicrography and scanning electron microscopy reveal that moving sponges possess distinctive leading edges composed of motile cells. Sponge locomotion was found to be mechanically similar to the spreading of cell sheets in tissue culture both with respect to exertion of traction (which causes the wrinkling of rubber substrata) and with respect to the patterns of adhesive contacts formed with the substratum (as observed by interference reflection microscopy). Other similarities include the orientation of sponge locomotion along grooves and the preferential extension onto more adhesive substrata. Neither the patterns of wrinkling produced in rubber substrata nor the distributions of adhesive contacts seen by interference reflection microscopy show evidence of periodic, propagating waves of surface contractions, such as would be expected if the sponges' mechanism of locomotion were by peristalsis or locomotory waves. Our observations suggest that the displacement of sponges is achieved by the cumulative crawling locomotion of the cells that compose the sponge's lower surface. This mode of organismal locomotion suggests new explanations for the plasticity of sponge morphology, seems not to have been reported from other metazoans, and has significant ecological implications. 相似文献
13.
Electric field-directed fibroblast locomotion involves cell surface molecular reorganization and is calcium independent 总被引:3,自引:0,他引:3
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《The Journal of cell biology》1994,127(1):117-128
Directional cellular locomotion is thought to involve localized intracellular calcium changes and the lateral transport of cell surface molecules. We have examined the roles of both calcium and cell surface glycoprotein redistribution in the directional migration of two murine fibroblastic cell lines, NIH 3T3 and SV101. These cell types exhibit persistent, cathode directed motility when exposed to direct current electric fields. Using time lapse phase contrast microscopy and image analysis, we have determined that electric field-directed locomotion in each cell type is a calcium independent process. Both exhibit cathode directed motility in the absence of extracellular calcium, and electric fields cause no detectable elevations or gradients of cytosolic free calcium. We find evidence suggesting that galvanotaxis in these cells involves the lateral redistribution of plasma membrane glycoproteins. Electric fields cause the lateral migration of plasma membrane concanavalin A receptors toward the cathode in both NIH 3T3 and SV101 fibroblasts. Exposure of directionally migrating cells to Con A inhibits the normal change of cell direction following a reversal of electric field polarity. Additionally, when cells are plated on Con A- coated substrata so that Con A receptors mediate cell-substratum adhesion, cathode-directed locomotion and a cathodal accumulation of Con A receptors are observed. Immunofluorescent labeling of the fibronectin receptor in NIH 3T3 fibroblasts suggests the recruitment of integrins from large clusters to form a more diffuse distribution toward the cathode in field-treated cells. Our results indicate that the mechanism of electric field directed locomotion in NIH 3T3 and SV101 fibroblasts involves the lateral redistribution of plasma membrane glycoproteins involved in cell-substratum adhesion. 相似文献
14.
Kaplow LS Chevance Ad Ade B Henkel J Malawista SE 《Cell motility and the cytoskeleton》2002,53(4):289-292
The study was carried out under direct videomicroscopic control to ascertain whether electromagnetic forces (photons) can initiate directed cell motility of human polymorphonuclear neutrophils (PMN). Cell suspensions containing a mixture of randomly motile white blood cells and erythrocytes (red cells) were placed in a double-decked preparation created by a glass slide and two cover slips and sealed by paraffin. Erythrocytes in the upper or lower chamber were destroyed by a single burst from a narrow ruby laser beam. Directed locomotion of PMN toward the erythrocyte debris occurred exclusively in the chamber in which the erythrocytes had been destroyed. Only random PMN locomotion was observed in the adjacent chamber. The results indicate that in this experimental model, electromagnetic forces do not initiate directed locomotion. 相似文献
15.
Spontaneous and chemoattractant-induced oscillations of cytosolic free calcium in single adherent human neutrophils 总被引:9,自引:0,他引:9
M E Jaconi R W Rivest W Schlegel C B Wollheim D Pittet P D Lew 《The Journal of biological chemistry》1988,263(22):10557-10560
Studies with fluorescent Ca2+ indicators in large populations of neutrophils in suspension reveal a stable base line followed by a rapid agonist-induced elevation of cytosolic free calcium, [Ca2+]i, concomitant with other parameters of cellular activation. To study the role of adhesion in cell activation, we monitored [Ca2+]i in single neutrophils adhered to albumin-coated or fibronectin-coated glass coverslips before and after stimulation with the chemotactic peptide N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP). Human neutrophils loaded with 2 microM fura 2/AM were allowed to adhere to coverslips for 15-20 min at 37 degrees C. [Ca2+]i was monitored with a dual excitation microfluorimeter with a time resolution of 200 ms. Statistical analysis was performed using an algorithm allowing to detect significant [Ca2+]i peaks. 54% of the cells showed spontaneous [Ca2+]i oscillations. The amplitude of these [Ca2+]i peaks averaged 77 +/- 10 nM above basal levels (mean value of 110 +/- 20 nM), and their mean duration was 28 +/- 5 s; periods of [Ca2+]i bursts could last up to 15 min. In "silent" cells exhibiting a stable [Ca2+]i base line without spontaneous oscillations, low concentrations of fMLP (10(-10)-10(-9) M) could induce sustained [Ca2+]i oscillations. By contrast, higher agonist concentrations (10(-6) M) induced a single [Ca2+]i transient followed by a stable base line. 47% of the cells showing spontaneous [Ca2+]i oscillations did not respond to fMLP. Spontaneous [Ca2+]i oscillations depended on the continuous presence of extracellular Ca2+. Therefore: (i) spontaneous oscillations of [Ca2+]i occur in neutrophils adherent to various substrata; (ii) these oscillations do not preclude and can be dissociated from the response to fMLP; (iii) neutrophil functions might be controlled by [Ca2+]i oscillations rather than by sustained alterations of [Ca2+]i. 相似文献
16.
Most of the studies dealing with cellular shape, surface configuration, and motility are carried out in vitro on plane substrata. During the past years, the direct transfer of results obtained under these conditions to the cellular behavior displayed in the living organism, has been increasingly challenged. For this reason we have investigated the above mentioned functions of different cell classes localized on glass and in the loose connective tissue. The cells utilized were: fibroblasts and macrophages from normal rat and rabbit mesenteries, V2 rabbit carcinoma cells and L5222 rat leukemia cells. The combination of time-lapse cinematography and scanning electron microscopy revealed that motility and surface features are the same, irrespective of the immediate surrounding. Cellular shape and attachment, on the other hand, are dependent on the substrate. Fibroblasts, macrophages and cells of epithelial origin, including carcinoma cells, flatten on glass, but have a rounded configuration in the tissue. The flat leading lamellae displayed during locomotion on glass, are not evident in cells migrating through tissues. What regards attachment devices, extensively studied on glass, their formation and position within a tissue are, at present, a matter of speculation. Although it can be assumed that a similar process is operable in vivo and in vitro, clarification rests upon the use of ultrahistochemical techniques. 相似文献
17.
Aggregation of hepatocytes in culture is an important phenomenon to control in tissue engineering applications. Aggregation generally enhances maintenance of differentiated functions but inhibits cell growth. At present there exists insufficient information for rational design of substrata that control aggregation. Indeed, the cellular mechanism(s) underlying the aggregation process is poorly understood, although cell motility is generally considered to be an essential phenomenon. In this article we provide the first study investigating the relationship between hepatocyte aggregation and motility behavior on various extracellular matrix substrata, including Matrigel, laminin, and fibronectin. We find that the extent of aggregation depends on the concentration of the extracellular matrix proteins, as well as on the type. Furthermore, we find that the extent of aggregation appears to be independent of classical single-cell locomotion. In fact, under conditions giving rise to substantial aggregation, the fraction of cells exhibiting classical locomotion is essentially negligible. Instead, aggregation appears to involve intracellular contacts accomplished via a different form of cell motility: active cell membrane extensions followed by adhesive cell-cell interactions. An implication of these findings is that aggregation may be largely governed by relative strengths of cell-cell versus cell-substratum interactions. These observations could be helpful for improved design of cell transplantation devices and cell culture substrata. (c) 1996 by John Wiley & Sons, Inc. 相似文献
18.
Sroka J von Gunten M Dunn GA Keller HU 《The international journal of biochemistry & cell biology》2002,34(7):882-899
We characterised two sublines of Walker carcinosarcoma cells generated by epigenetic changes. Subline 1 cells were mostly polarised and made no or only non-adhesive cell-substratum contacts. Subline 2 cells were spread, adhesive and mainly non-polar. Subline 1 cells migrate in a non-adhesive mode which is very efficient but operates only in a 3D environment, whereas subline 2 cells migrate in an adhesive mode, which is less efficient but works on 2D and 3D substrata. Nocodazole had little or no effect on shape, polarity and locomotion of subline 1 cells. In glass-adherent subline 2 cells, 10(-6)M nocodazole increased the proportion of polarised cells migrating in an adhesive mode and decreased adhesion to the substratum, whereas 10(-5)M nocodazole further reduced the contacts and the cells reverted to a non-adhesive mode of locomotion. When non-polar subline 2 cells were detached mechanically or by nocodazole, they became polarised and morphologically indistinguishable from non-adherent subline 1 cells. On more adhesive plastic substrata, subline 2 cells produced heterogeneous responses to nocodazole including loss of polarity. The phenotypes of Walker carcinosarcoma sublines have similarities with a broad range of cell types ranging from leucocytes to fibroblast-like cells, suggesting that these phenotypic differences can be controlled by the adhesive and contractile state rather than the cell type. Adhesion modulates contractility (isometric or isotonic contraction) and vice versa and this determines morphology (shape, F-actin, myosin and alpha-actinin), locomotion and responses to microtubule-disassembly. The model may be applied to analyse the mechanisms controlling the phenotype of cells in general. 相似文献
19.
Endothelial cells subjected to cyclic stretching change orientation so as to be aligned perpendicular to the direction of applied strain in a magnitude and time-dependent manner. Although this type of response is not the same as motility, it could be governed by motility-related factors such as substratum adhesiveness and actin-myosin contractile level. To examine this possibility, human aortic endothelial cells (HAEC) were uniaxially, cyclically stretched on silicone rubber membranes coated with various concentrations of fibronectin, collagen type IV and laminin to produce differing amounts of adhesiveness (measured using a radial flow detachment assay). Cells were subjected to 10% pure cyclic uniaxial stretching for three hours at a rate of 10%/sec. Time-lapse images revealed that cells underwent large morphological changes without moving. For each type of protein there was a parabolic dependence on initial adhesiveness with optimal cell orientation occurring at very similar adhesive strengths. The effect of actin-myosin contractile level was examined by stretching cells treated with different doses of 2,3-butanedione monoxime (BDM) and Blebbistatin. Each drug induced a dose-dependent decrease in orientation angles after three hours of cyclic stretching. Furthermore, cell and stress fiber orientations were tightly coupled for untreated and Blebbistatin-treated cells but were uncoupled for BDM-treated cells. Even though orientation response to cyclic stretching is not a spontaneous motile response, it is determined, in large part, by the same factors that affect spontaneous motility--the cell-substratum adhesiveness and actin-myosin contractile level. 相似文献
20.
Motility of cultured fish epidermal cells in the presence and absence of direct current electric fields 总被引:9,自引:5,他引:4
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The motile behavior and cytoskeletal structures of fish epidermal cells (keratocytes) in the presence and absence of direct current (DC) electric fields were examined. These cells spontaneously show highly directional locomotion in culture, migrating at rates of up to 1 micron/s. When DC electric fields between 0.5 and 15 V/cm are applied, single epidermal cells as well as cell clusters and cell sheets migrate towards the cathode. Cell clusters and sheets break apart into single migratory cells in the upper range of these field strengths. Cell shape and morphology are unaltered when the keratocytes are guided by an electric field. Neither the spontaneous locomotion nor the electrically guided motility were found to be microtubule dependent. 1 mM La3+, 10 mM Co2+, 50 microM verapamil, and 50 microM nitrendipine (calcium channel antagonists) reversibly inhibited lamellipod formation and cell locomotion in both spontaneously migrating and electrically guided cells. Ciba-Geigy Product 28392, which stimulates the opening of calcium channels, and is a competitive inhibitor of nitrendipine, has no effect on the locomotion of keratocytes. Cell motility was also unaffected by hyperpolarizing and depolarizing (low and high K+) media. It is argued that while a tissue cell may accommodate changes in resting membrane potential without becoming more or less motile, the cell may not be able to counterbalance the effects of depolarization and hyperpolarization simultaneously. In this context, a gradient of membrane potential, which is induced by an external DC electric field, will serve as a persistent stimulus for cell locomotion. 相似文献