共查询到19条相似文献,搜索用时 70 毫秒
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一种蜘蛛基因组DNA的简易提取方法 总被引:6,自引:0,他引:6
本文介绍了1种改进的蜘蛛基因组DNA的提取方法.通过与传统DNA提取方法的比较,本方法具有可在常温条件下进行、DNA得率高、简便、经济等优势. 相似文献
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为了探索家蝇幼虫抗菌物质的提取方法,本文采用醋酸提取蝇蛆的抗菌物质,通过加热去除其中不稳定的蛋白成分,再通过切向流超滤和直接冻干法获得复合抗菌物质,测定其中可溶蛋白的含量、抗菌组分的抗菌活性及组成的差异。结果表明,和冻干法相比,利用切向流超滤法可以显著提高抗菌复合物质中可溶蛋白的含量。利用切向流超滤浓缩制备的抗菌物质和直接冻干制备的抗菌物质经过凝胶过滤层析及抗菌活性分析表明,非蛋白组分(A20-A21和B6-B12)比蛋白组分(A1-A2、A10-A13、B1-B2)抗菌谱广。电泳分析蛋白组分表明,其抗菌蛋白组分处在6 k Da-35 k Da的某些蛋白组分中。 相似文献
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松突圆蚧基因组DNA提取方法的比较 总被引:1,自引:0,他引:1
分别采用醋酸钾(KAc)法、十二烷基硫酸钠-蛋白酶K(SDS-PK)消化法、十六烷基三乙基溴化铵(CTAB)法及DNA提取试剂盒(吸附柱型)对单只松突圆蚧的基因组DNA进行提取。同时针对盾蚧科昆虫的特点,在提取前利用氯仿和解剖针去除样品表面的介壳。结果表明,用同种提取方法提取的经氯仿处理与未经处理样本的提取效果无明显差异,而采用解剖针去除介壳的样本提取效果较好。所采用的4种提取方法均能从新鲜标本中提取出DNA,其中CTAB法提取的DNA量较多,而SDS-PK法提取的DNA质量较好。 相似文献
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DNA extraction techniques that employ the reversible binding of DNA to silica via chaotropic salts can deliver high-quality genomic DNA from plant and animal tissues, while avoiding the use of toxic organic solvents. Existing techniques that use this method are either prohibitively expensive, or are applicable to only a restricted set of taxa. Here we describe a cost-effective DNA extraction technique suitable for a wide range of plant and animal taxa that yields microgram quantities of high-molecular-weight genomic DNA at a throughput of 192 samples per day. Our technique is particularly robust for tissue samples that are insoluble or are rapidly discoloured or oxidized in standard DNA extraction buffers. We demonstrate the quality of DNA extracted using this method by applying the amplified fragment length polymorphism technique to plant species. 相似文献
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In this protocol, we present a standard method for extracting DNA from cells of the social amoeba Dictyostelium discoideum. While this procedure is similar to other phenol:chloroform-based purification methods, it is modified to account for the high level of carbohydrate and nucleases found in Dictyostelium cells. Genomic DNA can be isolated from wild-type and genetically modified cells using the described protocol, allowing molecular genetic analyses to be performed. Following cell lysis, nucleic acid extraction, and precipitation, the isolated DNA is suitable for digestion by restriction enzymes, amplification by PCR and Southern blotting. This procedure takes approximately 3 h to complete. 相似文献
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一种优化的植物总DNA提取方法 总被引:21,自引:2,他引:19
根据自己提取植物基因组DNA的实际经验,改进了Clark利用CTAB提取植物基因组DNA的方法。在试验过程中发现,通过在研磨材料时加入液氮、用剪去端部的吸头转移含有DNA的溶液,并且将加入RNase A消化RNA的步骤改在最后进行等一系列改进,可以获得高质量的DNA。本文同时就主要提取步骤进行了分析。 相似文献
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Filippo Geuna Chiara Maitti Simona Digiuni Riccardo Banfi 《Plant Molecular Biology Reporter》2004,22(1):87-87
Isolating nucleic acids from sources rich in contaminants is particularly cumbersome when treating a large number of samples.
Several protocols have been published that address the problem of nucleic acid extraction and purification, but few address
sample number. We describe a method for extracting DNA from recalcitrant tree species by using a commercial grinding apparatus.
This alleviates the hard work of sample preparation prior to lysis and purification. Our method has been tested extensively
on different fruit tree species and in projects that require the simultaneous processing of hundreds of samples. Moreover,
it does not require the availability of robotic workstations. 相似文献
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