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1.
A U Larkman 《Tissue & cell》1984,16(3):393-404
The appearance and arrangement of the mitochondria during all stages of oocyte growth in the sea anemone Actinia fragacea (Cnidaria: Anthozoa) have been examined by electron microscopy. In small oocytes, the mitochondria are generally squat, with a dense matrix and numerous cristae, although a proportion may show an unusual arrangement of prismatic cristae. During early oogenesis, the mitochondria tend to be arranged in aggregates rather than randomly scattered, and may be associated with nuage material. With the onset of vitellogenesis, a large mitochondrial aggregate forms next to the nucleus. During early vitellogenesis this aggregate enlarges and comes to resemble the mitochondrial clouds found in some amphibian oocytes. Within the cloud, many mitochondria appear to be highly elongate and irregular in shape. The cloud begins to fragment and disperse midway through vitellogenesis at about the time when cortical granules appear. In fully grown oocytes, some mitochondria may have a much less dense matrix and fewer cristae than the remainder, which may be related to their state of activity.  相似文献   

2.
The organization of the chondriome and the ultrastructure of mitochondria have been studied in eggs and embryos of the sea urchin Paracentrotus lividus. The egg chondriome is characterized by an arrangement in well-delimited clusters. Analysis of mitochondrial clusters on electron micrographs of ultrathin serial sections shows two kinds of mitochondria of different shapes, the rod-shaped and the spherical. The egg mitochondria have a dense matrix and a well-ordered arrangement of cristae which, in rod-shaped variety, are perpendicular to the major axis. Cell division is accompanied by significant changes in intracellular distribution of mitochondria and in their structure. At the stage of 2-4 blastomeres, the clusters break up and numerous mitochondrial rods show signs of fragmentation; most of the observable mitochondria are of spherical shape. At the same time, the matrix becomes less dense, and the orderly arrangement of the cristae disappears. From the blastula to the gastrula stage, the observed modifications are reversed: the number of spherical-shaped mitochondria decreases, while that of the rod-shaped increases; the diameter of the latter is almost equal to the initial diameter of the spherical forms, the matrix becomes dense again and the cristae resume their orderly arrangement.  相似文献   

3.
Summary A variety of inclusion bodies occur in the mitochondria of several cell types of the snake, Elaphae quadrivirgata. These lie in the mitochondrial matrix or within the space of the cristae. The inclusions in the matrix are as follows: dense homogeneous and fine granular materials, structures with finger-print appearance and with filamentous and/or crystalloid pattern and fine ring-shaped and/or microtubular structures. The inclusions within mitochondrial cristae are glycogen particles, globular materials, and strand-like structures.These inclusions occur not only during the hibernation period of the snake, but also in the arousal period. Furthermore, some inclusions are encountered in fetal tissues. The functional significance of these inclusions is unknown; however, the present study suggests that they are related to the metabolic activity of the cells.  相似文献   

4.
Alpha and beta cells can be distinguished by differences in mitochondrial and secretion granule structure. Many mitochondria of alpha cells possess “tubular” or prismatic cristae oriented longitudinally and having triangular profiles in cross-section. The matrix is particulate, with the roughly spherical particles measuring about 100 A in diameter. Evidence is presented indicating that alpha and beta granules are sequestrated in association with Golgi elements. Fully-condensed beta granules, assumed to be insulin, appear homogeneously dense and crystalline. Recurrent profiles of crystalline beta granules suggest that they possess an octahedral configuration. Alpha cell granules also appear homogeneously dense but have round profiles. Many acinar cell nuclei in both normal and alloxan-treated pancreata display masses of moderately dense fibrils oriented roughly parallel to each other. These fibrils are about 200 A across and their terminal portions are rebranched and often appear to be continuous with the finely granular or filamentous component of the nucleoplasm. Not infrequently the fibrils show evidence of periodicity. Alloxan has a specific destructive effect on beta cells. An initial effect seems to be the disruption and coalescence of the bounding membranes of beta granules. Lysosome-like bodies are often seen in beta cell cytoplasm, which ultimately becomes degranulated and necrotic following prolonged administration of large doses of alloxan.  相似文献   

5.
Summary Mitochondrial abnormalities are reported in four cases of phaeochromocytoma. These abnormalities include swelling and scant cristae, intramitochondrial dense bodies, septate-like junctions, intercristal fusion plus spheroidal bodies, and intramitochondrial rodlets. These structural mitochondrial changes are associated with reduction in activity of the mitochondrial enzymes, monoamine oxidase and succinic dehydrogenase.Supported by a grant-in-aid from the National Health and Medical Research Council  相似文献   

6.
"Intramitochondrial filamentous bodies" (IMFB) were occasionally found within the matrix of some mitochondria of the thick limb of Henle of the rat kidney, but not elsewhere in the tubular system. Three types were recognized: type I, an accumulation of filaments 55 A thick; type II, a bundle of parallel filaments having the same thickness as those of type I and regular spacing, 87 A apart, from center to center; and type III, consisting of type II with regular light bands of 280 A periodicity and a helical border of prismatic tubular cristae. In addition to these, electron-opaque masses showing variable and faint substructures were found in the matrix of mitochondria. It is suggested that all these IMFB may originate from mitochondrial cristae and that type II IMFB may be an intermediate developmental form between type I and type III. After uranyl acetate staining, IMFB and the membranes of prismatic tubular cristae showed highly increased electron opacity. The literature has been reviewed for reports of intramitochondrial filamentous inclusions in various types of cells. These inclusions have been classified according to their structural characteristics and the localization in the mitochondria and compared with IMFB reported herein.  相似文献   

7.
There have been several reports describing paracrystalline arrays in the intermembrane space of mitochondria. On closer inspection these structures appear to be junctions of two adjoining membranes. There are two types. They can be formed between the outer and inner mitochondrial membranes (designated outer-inner membrane junctions) or between two cristal membranes (intercristal membrane junctions). In rat heart, adjoining membranes appeared associated via a central dense midline approximately 30 Å wide. In rat kidney, the junction had a ladder-like appearance with electron-dense "bridges" approximately 80 Å wide, spaced 130 Å apart, connecting the adjoining membranes. We have investigated the conditions which favor the visualization of such structures in mitochondria. Heart mitochondria isolated rapidly from fresh tissue (within 30 min of death) contain membrane junctions in approximately 10–15% of the cross sections. This would indicate that the percentage of membrane junctions in the entire mitochondrion is far greater. Mitochondria isolated from heart tissue which was stored for 1 h at 0°–4°C showed an increased number of membrane junctions, so that 80% of the mitochondrial cross sections show membrane junctions. No membrane junctions are observed in mitochondria in rapidly fixed fresh tissue or in mitochondria isolated from tissue disrupted in fixative. Thus, the visualization of junctions in the intermembrane space of mitochondria appears to be dependent upon the storage of tissue after death. Membrane junctions can also be observed in mitochondria from other stored tissues such as skeletal muscle, kidney, and interstitial cells from large and small intestine. In each case, no such junctions are observed in these tissues when they are fixed immediately after removal from the animal. It would appear that most studies in the literature in which isolated mitochondria from tissues such as heart or kidney were used were carried out on mitochondria which contained membrane junctions. The presence of such structures does not significantly affect normal mitochondrial function in terms of respiratory control and oxidative phosphorylation.  相似文献   

8.
The fine structure of Botrytis fabae conidia was studied usinga variety of electron-microscope techniques. The spore walllacks conspicuous ornamentation and consists of microfibrilsembedded in a granular matrix. The two distinct wall layersseen in chemically fixed sections cannot be detected in cross-fracturedreplicas; the two layers are probably structurally similar.The outer surface of the plasmalemma is covered with branchedinvaginations and two kinds of particles. Three distinct typesof particles are present on the inner surface of the plasmalemma.In freeze-etched replicas nuclei, vacuoles, and other organellesalways appear smoothly rounded. Small vesicles pass throughthe plasmalemma into the cell wall. Particles approximately10 nm in diameter occur in compact rows on the cristae of cross-fracturedmitochondria: dense spherical particles, probably of calciumphosphate, are present in chemically fixed mitochondria. Prevacuolesand vesicles with membranous inclusions can be seen in bothcross-fractured replicas and chemically fixed sections. In cross-fracturedreplicas vacuoles and lipid bodies are frequently joined bystrands of endoplasmic reticulum.  相似文献   

9.
The ultrastructure and respiratory activity of mitochondria in rice coleoptile grown in the presence and in the absence of an inhibitor of mitochondrial protein synthesis (chloramphenicol) have been studied. It is shown that during the first 48 h of germination a rapid development of mitochondrial cristae takes place without notable influence of chloramphenicol on biogenesis of mitochondria. But the presence of the inhibitor has a significant effect in the subsequent period (48–144 h): a gradual and almost complete reduction of mitochondrial cristae is observed. These unusual “noncristate” mitochondria, although greatly lacking cytochrome oxidase, have a high respiratory activity. The respiration of “noncristate” mitochondria is resistant to KCN. It is supposed that chloramphenicol-induced rice coleoptile can be used as a new convenient object for studies of the nature of alternative oxidase as well as the biogenesis of mitochondria with cyanide-insensitive respiration.  相似文献   

10.
The submicroscopic structure of the growing surface of the shell of the oyster, Crassostrea virginica, was studied by means of shadowed replicas. The outer edge of the prismatic region consists of a fine grained matrix enclosing crystals, the surfaces of which show a finely pebbled structure. Crystal size varies continously from 0.01 µ to 8 µ. The matrix surface shows no evidence of fibrous structure. The outer portions of the prismatic region exhibit a tile-like arrangement of large crystals separated by granular matrix 0.02 to 0.08 µ in thickness. The exposed crystal surfaces have indentations of varying form which appear as roughly parallel grooves spaced at intervals of approximately 0.3 µ. The final form of this region is believed to result from the random distribution of crystal seeds, which grow without orientation and through coalescence and growth come into contact, producing polygonal areas. The crystal arrangement of the nacreous region is one of overlapping rows of crystals in side to side contact, and with one end of each crystal free, permitting continued increase in length. Crystal angles and plane indices are presented.  相似文献   

11.
Following the application of high pressure to skeletal muscle for extended periods, intracristal structures are found in the mitochondria. In addition, electron dense granules of 70–170 nm diameter are found in the matrix of these mitochondria. In contrast, pressure-treated liver mitochondria show only large (300–400 nm) matrix granules but not the intracristal structures. Both the inner and outer mitochondrial membranes appear intact after pressure-treatment. Short periods of pressuretreatment have little effect on either the morphology of mitochondria or the pH of the tissues. It is suggested that the formation of the intracristal structures may be due to the effects of pH rather than pressure alone. This finding raises the possibility that intracristal structures may occur as a preparative artefact particularly where the tissue has undergone considerable manipulation.  相似文献   

12.
泥螺卵黄发生过程中线粒体的变化   总被引:11,自引:1,他引:10  
应雪萍  杨万喜 《动物学研究》2001,22(5):T001-T002
利用透射电镜(TEM)技术研究了泥螺卵黄发生过程中线粒体的形态结构的变化特点,结果表明,从卵黄发生早期到晚期,卵母细胞内线粒体经历了从外部形态到内部结构的一系列变化。卵黄合成初期的卵母细胞内,线粒体多,结构典型,仅部分线粒体外膜破裂,嵴 和内膜逐渐消失,卵黄发生中期,线粒体基质空泡化,嵴和内膜消失,腔内充满颗粒状物质,最后演变成卵黄颗粒,随着卵母细胞的发育,卵黄颗粒的数量和直径逐渐增加,卵黄发生后期,卵质中胞器不发达,细胞质中充满卵黄颗粒,在卵黄颗粒之间仅有少量线粒体存在,提供细胞代谢所需的能量,此外,对线粒体在卵黄形成中的功能,去向及行为变化等 进行了讨论。  相似文献   

13.
SYNOPSIS. Comparisons are made between sectioned and negatively-stained mitochondria of the ciliate Spirostomum ambiguum. Particulate elements 70–80 A in diameter are associated with the surface of tubular cristae of negatively-stained and disrupted mitochondria; such particles are not seen in sectioned mitochondria fixed in various ways. As measured in sectioned material, the inner mitochondrial membrane forming the tubular cristae is about 100 A thick, while the outer mitochondrial membrane is about 50 A thick and is the more labile of the 2.  相似文献   

14.
Summary

Within the unpaired testis, spermatogonia, spermatocytes, spermatids and spermatozoa were found. In early spermatids, mitochondria take perinuclear positions and centrioles a diplosomal arrangement. Rootlet-like striated differentiations occur in slightly more advanced stages. Then a conical cytoplasmic projection develops, supported by a single row of closely spaced microtubules. At this stage of maturation, giant Golgi stacks occur within the cytoplasm of the cytophore which is rich in different elongate structures and oval dense bodies. With progressive differentiation, the nucleus elongates and its chromatin condenses into twisted lamellae. Two centrioles, which change their diplosomal configuration and come to lie in line to each other, and rootlet-like structures remain near the tip of the median cytoplasmic outgrowth. Mitochondria start to fuse into a single long cylindrical mitochondrial rod extending beside the lengthening nucleus. Bone-shaped rods, smaller dense sticks and dense bodies migrate into the outgrowth. Spermatozoa are totally ensheathed by cortical microtubules. These tubules show different arrangements along the cell body. The thread-like nucleus extends along the cell, the first quarter excepted, whereas the single mitochondrion extends over two thirds of the cell. Two strings with linearly arranged oval dense bodies run in the median to post-median cell segment; four rows of bone-shaped rods and two rows of smaller electron-dense sticks extend from the frontal end up to the beginning of the last third of the cell. All the different longitudinal cords run in the gaps between 4 sets of microtubules. Ciliary axonemes or lateral bristles were not observed. The present findings substantiate the hypotheses, that spermatozoa in the Macrostomida are aciliate and that Myozona takes an isolated position within the Macrostomidae. The occurrence of two centrioles, which come to lie in line to each other and which stay in the tip of the cytoplasmic outgrowth in spermatids, may indicate that biciliate spermatozoa are characteristic for the Rhabditophora (= Macrostomorpha+Trepaxonemata) and not an evolutionary novelty of the Trepaxonemata.  相似文献   

15.
Summary Coated membranes in two types of gill epithelial cell of adult lamprey, Lampetra japonica, were studied by electron microscopy. The type 3 gill epithelial cells possess well-developed microvilli or microfolds, apical vesicles and abundant mitochondria. The cytoplasmic surface of the microvillous plasma membrane is covered by a coat of regularly spaced particles with a center-to-center distance of about 15 nm. Each particle consists of a bulbous free end, about 10 nm in diameter, and a connecting piece, about 5 nm long. Apical vesicles are covered by a surface coat which consists of fine filamentous material but lack any special coating on their cytoplasmic surface.The type 4 cells (chloride cells) are characterized by apical vesicles, abundant mitochondria and cytoplasmic tubules. These tubules possess a coat on their luminal surface which consists of spirally wound parallel rows of electron-dense materials. The rows are about 16 nm apart and wound at a pitch of about 45°. The cytoplasmic surface of these tubules does not display a special coat. These coated membranes are assumed to be the sites of active ion transport across the plasma membrane. In particular, particles in type 3 cells and linear coat materials in chloride cells may be either loci of transport enzymes or energy generating systems. Apical vesicles lack any coating on their cytoplasmic surface but a fine filamentous coat is present on their luminal surface. They contain intraluminal vesicles and are continuous with apical ends of cytoplasmic tubules.  相似文献   

16.
MICROCYLINDERS WITHIN MITOCHONDRIAL CRISTAE IN THE RAT PINEALOCYTE   总被引:2,自引:2,他引:0       下载免费PDF全文
A minute cylindrical structure with a dense core, designated as "microcylinder," has been observed within enlarged spaces of mitochondrial cristae in pinealocytes of some adult rats (osmium tetroxide fixed, methacrylate embedded). The microcylinders are 270 to 330 A in diameter and of indeterminate length. Their wall is found to be made up of slender filamentous subunits, probably 6 in number, and surrounds a central filament. The microcylinders are arranged parallel to one another, forming monolayered or, more frequently, multilayered aggregates. Their number within a crista varies considerably. Packets of microcylinders may be seen located in the outer mitochondrial chamber, but are never found in the mitochondrial matrix. They have been observed neither in other cell types of the pineal gland nor in neurons, ependymal, and glial cells of the nearby epithalamic tissue. The origin and nature of the microcylinders are unknown. Glycogen-like particles have been encountered, though very infrequently, accompanying bundles of microcylinders within mitochondrial cristae.  相似文献   

17.
Physical fixation by rapid freezing followed by freeze-fracture and deep-etching has provided the means for potentially seeing the three-dimensional arrangement in the native state of particles on mitochondrial inner membranes. We have used these techniques to study the tubular cristae of Paramecium in the hope of determining the arrangement of F1 complexes, their abundance, and location in the membranes. We also sought information regarding other respiratory complexes in these membranes. Our results, supported by stereo pairs, show that F1 complexes are arranged as a double row of particles spaced at 12 nm along each row as a zipper following the full length of the outer curve of the helically shaped tubular cristae. There are an average of 1,500 highly ordered F1 complexes per micrometer squared of 50-nm tubular cristae surface. The F1 complexes definitely lie outside the membranes in their native state. Other particle subsets, also nonrandomly arrayed, were seen. One such population located along the inner helical curve consisted of large 13-nm-wide particles that were spaced at 30 nm center-to-center. Such particles, because of their large size and relative abundance when compared to F1 units, resemble complex I of the respiratory complexes. Any models attempting to understand the coupling of respiratory complexes with F0F1 ATPase in Paramecium must take into account a relatively high degree of order and potential immobility of at least some of these integral membrane complexes.  相似文献   

18.
Some characteristics of the mitochondria of hepatocytes and of three hepatoma cell lines have been compared. By means of stereologic analysis of electron micrographs of cross-sections through cells the volume of mitochondria per unit volume of cell cytoplasm and the surface areas of the mitochondrial envelope and cristae membranes have been measured. The relative mitochondrial volume in the cytoplasm decreases with increasing growth rate but the surface area of outer and cristae membranes per unit volume of mitochondria is not altered. The internal organization of hepatoma mitochondria, however, differs distinctly from that of normal liver mitochondria as evident from electron micrographs; the hepatoma cells contain mitochondria in which parallel cristae appear to cross the whole mitochondrial profile unlike the irregular, short cristae seen in normal liver mitochondria. Furthermore, in the fast-growing hepatoma cells the mitochondrial matrix appears less dense than in the hepatocyte. Hepatoma cells contain less organized rough endoplasmic reticulum than normal liver cells and the spatial relationship of the mitochondria to the rough cisternae, seen in the hepatocyte, is absent in the fast-growing hepatoma cell lines. It is concluded that hepatoma cells have fewer mitochondria than normal liver cells, but that the organelles have a normal content of inner membranes.  相似文献   

19.
Summary Isolated rat liver and kidney mitochondria were swollen in hypotonie sucrose solutions and then examined in the electron microscope. The results were consistent with the hypothesis that unfolding of the cristae plays some part in swelling since cristae of swollen mitochondria appeared less abundant and with increased intercristal distances. The matrix became more electron-transparent. The fate of the outer membrane is uncertain.This investigation was supported by Public Health Service Research Grants GM-08900, NB-02145, Interdisciplinary Grant 5T1-MH-6418 and a Lederle Medical Faculty Award.  相似文献   

20.
This review summarizes recent findings from electron tomography about the three-dimensional shape of mitochondrial membranes and its possible influence on a range of mitochondrial functions. The inner membrane invaginations called cristae are pleomorphic, typically connected by narrow tubular junctions of variable length to the inner boundary membrane. This design may restrict intra-mitochondrial diffusion of metabolites such as ADP, and of soluble proteins such as cytochrome c. Tomographic images of a variety of mitochondria suggest that inner membrane topology reflects a balance between membrane fusion and fission. Proteins that can affect cristae morphology include tBid, which triggers cytochrome c release in apoptosis, and the dynamin-like protein Mgm1, involved in inter-mitochondrial membrane fusion. In frozen-hydrated rat-liver mitochondria, the space between the inner and outer membranes contains 10-15 nm particles that may represent macromolecular complexes involved in activities that span the two membranes.  相似文献   

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