人工饲养条件下恒河猴血液学、血液生化正常参考值测定及分析

目的测定人工饲养条件下104只健康恒河猴的血液学、血液生化正常参考值,并分析不同性别、不同年龄阶段(青幼年组∶3～4岁,成年组∶5～10岁,老年组∶11～25岁)恒河猴的血液学、血液生化值的差异及相互关系.方法应用全自动血液细胞分析仪和全自动血液生化分析仪,测定及分析非麻醉状态下健康恒河猴血液学和血液生化正常值.结果血液学值中较明显低 (P<0.05) 的项目为MED.血液生化值中♀猴比♂猴明显低 (P<0.01)的项目有ALT、ALP、GGT、CHOL、APOA、HDL、LDL、P3+、BUN,较明显低 (P<0.05) 的项目为ALB.较明显高 (P<0.05) 的项目为UA.各年龄组之间的分析如下:血液学值中RDW值为青幼年组比成年组及老年组均明显低(P<0.01),MEDIAN值为成年组比青幼年组较明显高 (P<0.05),血液生化值中青幼年组比成年组及老年组均明显高 (P<0.01)的项目有ALT、ALP、GGT、P3+,青幼年组比成年组明显高(P<0.01)的项目为BUN、ALP、AST、,青幼年组比老年组明显高(P<0.01)的项目为CK、TF;成年组比老年组明显低(P<0.01)的项目为CREAT,较明显低 (P<0.05) 的为TRIG.结论本文建立了人工饲养条件下健康恒河猴血液学和血液生化值的正常指标,并讨论了年龄、性别对其正常指标的影响,可供参考.     

无菌大鼠血液学及血液生化指标正常参考值的测定

目的人工培育Wistar无菌大鼠,观测F1和F2代无菌大鼠血液学及血液生化指标差异。方法对清洁级Wistar大鼠进行子宫摘除,隔离器内剥离子宫壁获得大鼠胎仔,人工哺育为无菌大鼠。待其繁殖后,采用全自动血液生化分析仪、全血细胞计数仪测定F1和F2代无菌大鼠的血液学及血液生化指标。结果不同代数无菌大鼠的血液学和血液生化指标存在显著差异,其中有差异的血液学指标包括RBC、HGB、HCT、MCV、MCHC、RDW、MPV、NE%、LYM%、EO%、BA%、NE#、LYM#、EO#、BA#（P〈0.05）;有差异的血液生化指标包括ALT、ALB、LDH、CHO、TBIL、HDL-C、LDL-C、GLO、GLU、ALP、A/G、UA、P（P〈0.05）。同时这些差异又因性别和年龄而不同。结论代数、性别、年龄对无菌大鼠的血液学及血液生化指标有较大影响。     

狼的血液学和血液生化学参考值

本文报道了８只狼４６项血液学和血液生化学有关参考值。结果表明,各项血液学和血液生化学参数值在成年个体间无性别差异（Ｐ＞０．０５）。与其他犬科动物血液生理生化参数值比较,狼除白细胞中的嗜酸性粒细胞和淀粉酶值明显高外,其他参数值虽有差异,但均无统计学意义。淀粉酶值高,提示与狼高强度运动习性所需机体内由葡萄糖提供能量来源的生理特性是相适应的。     

食蟹猴血液学及血清生化指标日间节律性变化

目的研究食蟹猴血液学及血清生化指标日间节律性变化,为正确全面评估血液学及血清生化测定结果提供一定的实验事实依据。方法在9：30（am）～10：30（am）、12：30（pm）～13：30（pm）、15：30（pm）～16：30（pm）及19：30（pm）～20：30（pm）4个时间段对12只食蟹猴（雌雄各半）采血。采用全自动血细胞分析仪及血液生化分析仪分别测定血液学及血清生化指标。利用高效液相色谱（HPLC）方法分析血浆中皮质醇含量。结果雌雄食蟹猴下午白细胞数（WBC）及尿素氮（BUN）显著升高;雌性食蟹猴下午血红蛋白（HGB）及血细胞压积（HCT）显著降低,天门冬氨酸氨基转换酶（AST）及丙氨酸氨基转换酶（ALT）活性显著升高;雄性食蟹猴下午血糖（GLU）及血浆皮质醇显著降低。结论雌雄食蟹猴血液学及血清生化部分指标存在明显的日间节律性。     

人工饲养树鼩血液学及生化指标正常值测定及分析

目的建立人工饲养健康树鼩血液学和血液生化指标的正常值参考范围,并探讨与不同物种的血液学和血液生化值的差异及相互关系。方法应用全自动血细胞仪和生化分析仪测定140只健康成年树鼩的血液学及血液生化指标。结果雌性与雄性比较,LYM％、PLT、RDW％、GLU、CREA的差异具有显著性（JP〈0．05）,11P、CHOL、TG的差异具有极显著性（P〈0．01）,其他指标的差异不具显著性,部分指标与恒河猴、大鼠及人的相应指标存在一定的差异。结论本文建立了健康树鼩的血液学及生化指标的正常值参考范围,可为应用该动物进行科学研究时提供参考。     

妊娠晚期食蟹猴与同龄未孕食蟹猴血液生理和生化指标的比较

目的提供妊娠晚期食蟹猴有关的血液学数据。方法采用Sysmex XE-2100血液分析仪、SysmexCA-7000凝血分析仪,及OL YMPUS AU600型全自动生化分析仪,对15只怀孕晚期食蟹猴及10只正常对照非孕食蟹猴进行血常规、凝血三项及17项血生化指标分析。结果妊娠晚期食蟹猴在WBC、NEU%、LYM%、PT、APTT、Fgb、TP、Alb、BUN、CR、CHOL、Glu、Fe、LDK、HDBH上,与同龄未孕食蟹猴相比差异显著（P〈0.05）,其他指标RBC、HGB、HCT、MCV、MCH、MCHC、PLT、ALT、AST、ALP、T.Bili、TG、P相比,于孕晚期有不同程度改变,但无显著性差异（P〉0.05）。结论初步建立了孕晚期食蟹猴的常规生理学数据,为相关的实验研究提供基础资料。     

白暨豚某些血液生化指标的测定

关于海豚和几种淡水豚类的血液学和血液生化指标国外有过研究。白暨豚(Lipotes vexillifer)血液有形成份已有过报道。本实验对白暨豚血液生化指标进行了研究,以期建立白暨豚血液正常生理生化指标,为白暨豚的临床诊断,健康监测和保健措施提供血液学参数,并且为完善淡水豚类血液学比较研究提供基础资料。     

雌雄金黄地鼠血液生理生化指标的比较分析

目的测定金黄地鼠的血液生理生化参考值,并对两性的参考值进行比较。方法将88只4周龄金黄地鼠,80只8周龄金黄地鼠,采血后,用日本光电MEK-7222K血球分析仪测定血液生理指标,用Olympus AU400全自动生化分析仪测定血清生化指标。结果4周龄金黄地鼠血液生理指标中RBC、HGB、HCT、MCV和RDW性别差异有显著性（P〈0.05）,而WBC、MCH、MCHC、PLT、PCT、MPV和PDW等性别差异无显著性（P〉0.05）;4周龄金黄地鼠血清生化指标中ALB、ALP、GLU、CA、P、CHO和TG性别差异有显著性（P〈0.05）,而ALT、AST、TP、BUN和CREA等性别差异无显著性（P〉0.05）。8周龄金黄地鼠血液生理指标中WBC、LYM、MON、BAS、LYM％、NEUT％、MCH、RBC、HGB、HCT、MCV、PDW和RDW性别差异有显著性（P〈0.05）,而MCHC、PLT、MPV、PCT、NEUT、EOS、BAS、MON％和EOS％等性别差异无显著性（P〉0.05）;8周金黄地鼠血清生化指标中ALT、ALB、BUN、GLU、CREA、P、CHO和TG性别差异有显著性（P〈0.05）,而AST、TP、ALP和CA等性别差异无显著性（P〉0.05）。结论金黄地鼠血液的生理生化值受多种因素影响,部分指标两性问测定值有差异,但多数指标差异不显著。     

猴免疫缺陷病毒（SIV）感染恒河猴血液学和血液生化的动态变化

目的探讨SIVmac239感染中国恒河猴的血液学和血清生物化学的变化,研究艾滋病的发生机制。方法 20只恒河猴感染SIVmac239后分别在感染前和感染后2、6、9、12、15和18个月在猴空腹状态用盐酸氯胺酮麻醉下静脉釆血。抗凝血用全自动血液细胞分析仪检测血液常规,血清用全自动生化分析仪检测生化指标。结果SIV感染猴红白细胞计数呈逐渐的减少。淋巴细胞计数有较明显减/增的波动。血清酶类ALT和AST没有明显的上升,但在15月时均有所下降P〈0.05～P〈0.01。LDH持续降低,原因未明。CK则逐月上升,至12月时达高峰,后又有所下降。血尿素氮逐月上升,并在6～18个月时P〈0.01。总蛋白量上升,白蛋白逐月降低,球蛋白升高,白蛋白/球蛋白比率倒置。SIV感染猴2个月后总胆固醇、甘油三脂、低密度脂蛋白和高密度脂蛋白均呈上升,至12月时略有下降,但到18月又上升。血糖（GLU）在SIV感染后逐月上升,后期上升更为明显,这可能与胰岛的损害有关。结论 SIVmac239感染猴后,通过SIV侵入相关的器官病变和体液调节失衡导致血液学和血液生物化学产生异常,从而提供有关猴艾滋病的一些基础资料,这将对研究人的艾滋病也许有所帮助。     

封闭群草原兔尾鼠正常血液生化指标的测定

利用雅培 (AEROSET)全自动血液生化测定仪对封闭群草原兔尾鼠 2 3项血液正常生化指标进行了测定。与昆明小鼠、BALB c nu小鼠、Wistar大鼠、人的正常值相比较 ,该种实验动物血液正常生化指标具有以下特点 :①总蛋白 (TPROT)、白蛋白 (ALB)、球蛋白 (GLOB)、总胆红素 (TBILI)、直接胆红素 (DBILI)、间接胆红素 (IBILI)、尿素氮 (UREA)、二氧化碳 (CO2 )、甘油三脂 (TG)、胆固醇 (CHOL)、钠 (Na)、氯 (Cl)、镁(Mg)、总钙 (TCA)的测定值 ,五者间无显著性差异 (P >0 0 5 ) ;②封闭群草原兔尾鼠与昆明小鼠相比 ,谷草转氨酶 (AST)、谷丙转氨酶 (ALT)、糖 (GLU)、钾 (K)、磷 (P3 + )存在显著性差异 (P <0 0 1或P <0 0 5 ) ;③封闭群草原兔尾鼠与BALB c nu小鼠相比 ,谷草转氨酶 (AST)、谷丙转氨酶 (ALT)、碱性磷酸酶 (ALP)、糖 (GLU)测定值 ,存在显著性差异 (P <0 0 1或P <0 0 5 ) ;④封闭群草原兔尾鼠与Wistar大鼠相比 ,谷草转氨酶、谷丙转氨酶、钾、糖测定值 ,存在显著性差异 (P <0 0 1或P <0 0 5 ) ;⑤封闭群草原兔尾鼠与人相比 ,谷草转氨酶、谷丙转氨酶、碱性磷酸酶、乳酸脱氢酶 (LDH)、肌肝 (CREAT)、尿酸 (UA)、钾 (K)存在显著性差异 (P <0 0 1或P <0 0 5 )。结果表明 :该种动物可能适合于作为研     

Reproductive seasonality in wild northern pig-tailed macaques (Macaca leonina)

Primates - Macaque reproductive patterns range from strictly seasonal breeding to non-seasonal breeding, but factors explaining this variation are not fully understood. Valid reproductive...     

Parasites may exit immunocompromised northern pig-tailed macaques(Macaca leonina) infected with SIVmac239

Parasites can increase infection rates and pathogenicity in immunocompromised human immunodeficiency virus(HIV) patients. However, in vitro studies and epidemiological investigations also suggest that parasites might escape immunocompromised hosts during HIV infection.Due to the lack of direct evidence from animal experiments, the effects of parasitic infections on immunocompromised hosts remain unclear. Here,we detected 14 different parasites in six northern pig-tailed macaques(NPMs) before or at the 50 th week of simian immunodeficiency virus(SIV) infection by ELISA. The NPMs all carried parasites before viral injection. At the 50 th week after viral injection, the individuals with negative results in parasitic detection(i.e., 08247 and 08287) were characterized as the Parasites Exit(PE) group, with the other individuals(i.e., 09203, 09211, 10205, and 10225) characterized as the Parasites Remain(PR) group. Compared with the PR group, the NPMs in the PE group showed higher viral loads, lower CD4~+T cells counts, and lower CD4/CD8 rates. Additionally, the PE group had higher immune activation and immune exhaustion of both CD4~+and CD8~+T cells. Pathological observation showed greater injury to the liver, cecum, colon,spleen, and mesenteric lymph nodes in the PE group.This study showed more seriously compromised immunity in the PE group, strongly indicating that parasites might exit an immunocompromised host.     

Analysis of immunoglobulin,complements and CRP levels in serum of captive northern pig-tailed macaques (Macaca leonina)

The northern pig-tailed macaque(NPM,Macaca leonina) has become a widely used animal model in biomedical research. In this study, we measured serum immunoglobulin IgG, IgM, IgA, complement C3, C4 and CRP levels in 3-11 year old captive northern pig-tailed macaques using HITACHI 7600-20 automated chemistry analyzer in order to determine the influences of age and gender on these items. The results showed that serum IgA, IgM, C3 and C4 levels were not correlated with age(P0.05), while serum IgG levels increased progressively with age(r=0.202;P=0.045). Serum IgG, IgA, IgM and C3 levels were higher in females than in males(P0.05). Moreover, serum C3 concentration was both positively and strongly correlated with that of C4(r=0.700; P0.0001). This study provides basic serum immunoglobulin and complement data of captive northern pig-tailed macaques, which may prove useful for future breeding efforts and biomedical research.     

Changes in blood chemistry and hematology variables during aging in captive rhesus macaques (Macaca mulatta)

Identifying changes with age in physiological variables of captive nonhuman primates will aid in the proper treatment and clinical diagnosis of these animals, as well as enhance our understanding of nonhuman primate models for human aging. Information for 33 physiological variables was obtained from the Primate Aging Database, a multi-centered database being developed for clinical and research use. Using multiple regression analyses, we investigated the relationship of age to hematological variables, blood chemistry and body weight in 345 captive rhesus monkeys (age range 7-30 years) from three different primate research facilities. The analyses revealed that 15 of these variables show a significant relationship with chronological age and are altered in older as compared with adult animals. Here we present the first phase of a project that will: a) identify changes with age in physiological variables among adult captive rhesus macaques; and b) characterize normative values for the aging rhesus population.     

Blood groups of pig-tailed macaques (Macaca nemestrina).

The human-type A-B-O blood groups of 57 pig-tailed macaques (Macaca nemestrina) were determined and the calculated gene frequencies, O = 0.8908, A = 0.0825 and B = 0.0267, gave excellent fit with the hypothesis of inheritance by triple allelic genes. In tests for simian-type blood groups with rhesus, baboon and crab-eating macaque immune antisera, it was shown that the red cells of pig-tailed macaques are polymorphic for several simian-type specificities defined by those cross-reacting sera. Pig-tailed macaques share with other macaque species the complex Drh-graded blood group system, which seems to occupy a special role among red cell antigens of macaques. Normal sera of three female macaques contained spontaneous isoagglutinins which selectively agglutinated the red cells of some pig-tailed as well as stump-tailed macaques.