中国围盾蚧属一新种（半翅目：蚧总科：盾蚧科）

记述中国围盾蚧属Fiorinia Targioni-Tozzetti 1新种:南宁围盾蚧Fiorinia nanningensis Zhang&Feng sp.nov.。该种近似于钩樟围盾蚧F.linderaeTakagi,1969,二者臀板均无腺刺分布,并且第2臀叶退化,略突起或全无,但是可以从以下特征区别:1)新种中臀叶狭小,末端截型,内缘无锯齿(钩樟围盾蚧中臀叶大而内缘多齿);2)躯干上无腺刺或腺瘤(钩樟围盾蚧后胸有1-3个腺瘤,第1腹节有3或4个腺瘤,第2腹节有1-2个腺刺);3)无触角间突(钩樟围盾蚧触角间突小圆锥形)。对其形态特征进行了详细描述,提供了玻片绘图。研究标本保存在西北农林科技大学昆虫博物馆。     

中国片盾蚧属一新种记述(半翅目:盾蚧科)

记述中国片盾蚧属1新种——勐仑片盾蚧Parlatoria menglunensis,sp.nov．。模式标本保存于西北农林科技大学昆虫博物馆。     

中国齿盾蚧属一新种(半翅目:蚧总科:盾蚧科)(英文)

记述中国齿盾蚧属Odonaspis Leonardi1新种——龙岩齿盾蚧Odonaspis longyanensis Wang et Feng,sp.nov.,编制了中国齿盾蚧属分种检索表,模式标本保存于西北农林科技大学昆虫博物馆。     

中国片盾蚧属一新种记述(半翅目:盾蚧科)

记述中国片盾蚧属1新种--蔷薇片盾蚧Parlatoria rosia,sp.nov..模式标本保存于中国科学院上海植物生理生态研究所昆虫标本馆.     

中国新栎盾蚧属一新种及二新纪录种(同翅目:蚧总科:盾蚧科)

记述新栎盾蚧属Neoquernaspis Howell et Takagi,1981一新种,即细管新栎盾蚧N.leptosipha sp.nov和2种中国分布新纪录种,即石柯新栎盾蚧N.beshearae Liu et Tippins,1988和尼泊尔新栎盾蚧N.nepalensis (Takagi,1977),并编制了该属中国已知种类检索表,新种模式标本和新纪录标本保存于西北农业大学昆虫博物馆。     

中国片盾蚧属一新种及一新纪录种(半翅目,盾蚧科)

记述中国片盾蚧属1新种芦苇片盾蚧Parlatoria reedia sp.nov和1新纪录种加氏片盾蚧Parlatoria ghanii Hall ＆ Williams,模式标本保存在中国科学院上海植物生理生态研究所昆虫标本馆.     

中国盾蚧科二新种记述(同翅目:盾蚧总科)

记述中国盾蚧科2新种：拟额瘤并盾蚧Pinnaspis pseudotuberculatua sp．nov．和云南兜盾蚧Duplachionaspis yunnanensis sp．nov．。模式标本保存在西北农林科技大学昆虫博物馆。     

美盾蚧属一新种记述（同翅目：蚧总科：盾蚧科）

记述美盾蚧属Formosaspis1新种：王朗美盾蚧F.wanglangensis,sp.nov.,模式标本采自四川省平武县王朗自然保护区的箭竹（hyllostachys bambusoides Sieb et Zacc）竹杆和分枝上。鉴别特征是雌成虫臀板背面有8个高度骨化的硬疤,围阴腺5群,每群数量远远多于同属其它种。二龄若虫的中臀叶与第3臀叶之间有缘管腺2对,第3臀叶的上侧有缘管腺3枚。     

安徽省盾蚧科一新属一新种记述(半翅目:蚧总科)

记述盾蚧科1新属——钝刺盾蚧属Mammilla,gen.nov.,并记述1新种:金寨钝刺盾蚧M.jinzhaiensis,sp.nov..模式标本保存于安徽工业大学.     

枝圆盾蚧属新种记述（同翅目：蚧总科：盾蚧科）

本文记述了枝圆盾蚧属Ｄｉａｓｐｉｄｉｏｔｕｓ Ｃｏｃｋｅｒｅｌｌ一新种,危枝圆盾蚧Ｄｉａｓｐｉｄｉｏｔｕ ｐｅｒｎｉｃｉａｂｉｌｕｓ ｓｐ．ｎｏｖ．,该属是盾蚧科Ｄｉａｓｐｉｄｉｄａｅ中的一个小属。新种的模式标本保存在中国科学院动物研究所。     

The identity of Amaranthus taishanensis F. Z. Li et C. K. Ni

Examination of the type specimens of Amaranthus taishanensis F. Z. Li et C. K. Ni shows that it is actually conspecific with A. polygonoides L., a North American species.     

泰山苋的名实问题

发现泰山苋 A．taishanensis F．Z．Li et C．K．Ni即为源于美洲的合被苋 A．Polygonoides L .,故于归并。     

Craniological differentiation between European wildcats (Felis silvestris silvestris), African wildcats (F. s. lybica) and Asian wildcats (F. s. ornata): implications for their evolution and conservation

Intraspecific diversification of the wildcat (Felis silvestris), including the European wildcat (F. s. silvestris), the Asian wildcat (F. s. ornata) and the African wildcat (F. s. lybica), was examined based on 39 cranial morphology variables. The samples of free‐ranging cats originated from Britain, Europe, Central Asia and southern Africa, consisting of both nominal wildcat specimens (referred to henceforth as ‘wildcats’) and nominal non‐wildcat specimens (‘non‐wildcats’) based on museum labels. The skull morphology of ‘wildcats’ from Britain and Europe is clearly different from that of ‘wildcats’ of Central Asia and southern Africa. The latter are characterized especially by their proportionately larger cheek teeth. On the basis of principal component, discriminant function and canonical variate analyses, the skull morphology of British ‘non‐wildcats’ is less distinct than is that of British ‘wildcats’ from the skull morphologies of ‘wildcats’ of Central Asia and southern Africa. On the other hand, the skull morphology of southern African ‘non‐wildcats’ is as distinct from those of ‘wildcats’ of Britain and Europe as is that of southern African ‘wildcats’. We suggest that the evolution of the modern wildcat probably consisted of at least three different distribution expansions punctuated by two differentiation events: the exodus from Europe during the late Pleistocene, coinciding with the emergence of the steppe wildcat lineage (phenotype of Asian–African wildcat), followed by its rapid range expansion in the Old World. The second differentiation event was the emergence of the domestic cat followed by its subsequent colonization of the entire world with human assistance. Considering the recent evolutionary history of, and morphological divergence in, the wildcat, preventing hybridization between the European wildcat and the domestic cat is a high conservation priority. © 2004 The Linnean Society of London, Biological Journal of the Linnean Society, 2004, 83 , 47–63.     

Purification and characterization of a thermostable cellobiohydrolase from Fomitopsis pinicola

A screening for cellobiohydrolase (CBH) activity was performed and Fomitopsis pinicola KMJ812 was selected for further characterization as it produced a high level of CBH activity. An extracellular CBH was purified to homogeneity by sequential chromatography of F. pinicola culture supernatants. The molecular mass of the F. pinicola CBH was determined to be 64 kDa by SDS-PAGE and by size-exclusion chromatography, indicating that the enzyme is a monomer. The F. pinicola CBH showed a t1/2 value of 42 h at 70 degrees C and catalytic efficiency of 15.8 mM-1 S-1 (kcat/ Km) for p-nitrophenyl-beta-D-cellobioside, one of the highest levels seen for CBH-producing microorganisms. Its internal amino acid sequences showed a significant homology with hydrolases from glycoside hydrolase family 7. Although CBHs have been purified and characterized from other sources, the F. pinicola CBH is distinguished from other CBHs by its high catalytic efficiency and thermostability.     

Molecular cloning of functional genes for high growth-temperature and salt tolerance of the basidiomycete Fomitopsis pinicola isolated in a mangrove forest in Micronesia

Several functional genes encoding putative proteins, heat shock protein 70, sphingosine phosphate lyase, and Na+/H+ antiporter, were cloned from the basidiomycete Fomitopsis pinicola, a wood-rotting fungus isolated in the tropical mangrove forest of Pohnpei Island of the Federated States of Micronesia. The deduced amino acid sequences of the obtained genes involved in heat shock resistance, lipid synthesis, and salt tolerance showed diverse similarities to other homologous proteins. Molecular phylogenetic trees of these proteins suggested that encoded proteins of the cloned genes of F. pinicola differed remarkably from other homologs in various organisms, even fungal proteins. Putative candidates for other genes related to several cellular metabolisms were also amplified, implying the possible existence of those genes in F. pinicola. This is the first report of possibly functional genes derived from a basidiomycetous mushroom growing in tropical islands such as Micronesia. The genes found in this study might play important roles in the cellular survival of the basidiomycete F. pinicola under severe environmental conditions.     

一种富集高温栅藻Desmodesmus sp. F51的新型生物絮凝剂

[背景]海科贝特氏菌(Cobetia marina)可产生大量具有絮凝活性的胞外产物,可视为一种新型的生物絮凝剂。高温栅藻(Desmodesmus sp.F51)是一种具有较高叶黄素含量的微藻,被认为是一种新兴的叶黄素来源,但利用该生物絮凝剂高效富集高温栅藻的相关研究迄今尚未见报道。[目的]以高温栅藻为对象,研究该新型生物絮凝剂的絮凝效率,并对絮凝机理进行初步探讨。[方法]探索在不同生长阶段微藻培养液添加生物絮凝剂、添加量、絮凝时间、pH对絮凝效率的影响,分析生物絮凝剂的功能基团,并测定在不同pH条件下添加生物絮凝剂前后高温栅藻的Zeta电位变化,以及在显微镜下分析藻细胞在添加生物絮凝剂前后的形态。[结果]在高温栅藻生长至稳定期(pH 8.0)添加2 mL生物絮凝剂,絮凝15 min絮凝效果最佳,达82.1%。傅里叶红外光谱(fourier transform infrared spectroscopy,FTIR)显示了多糖及酰胺结构的特征吸收峰,由此推测生物絮凝剂主要是多糖的混合物,含有少量蛋白质。根据Bradford法测定絮凝剂中蛋白含量约为0.4%(质量比),通过苯酚-硫酸法测定总糖质量分数约为34.5%(质量比),与FTIR分析结果基本相符。生物絮凝剂在pH 4.0-11.0保持60%以上的絮凝效率,说明无论是酸性或是碱性条件下絮凝效率都较高,结合Zeta电位的分析表明,推测生物絮凝剂对高温栅藻的絮凝机理中占主导地位的可能是吸附架桥作用。[结论]该研究对微藻生物絮凝具有重要的理论和实践意义。     

Characterization of microsatellite loci in the marine seaweeds,Fucus serratus and F. evanescens (Heterokontophyta; Fucaceae)

Fucus serratus and F. evanescens commonly occur on Northern European shores. Nine microsatellite loci were developed for F. serratus (8–22 alleles, observed heterozygosities = 0.367–0.850) and one for F. evanescens (seven alleles, observed heterozygosity = 0.804). Cross‐amplification was apparent, as five F. serratus loci were polymorphic in F. evanescens and 2–5 were polymorphic in F. vesiculosus, F. distichus, and F. spiralis.     

薜荔和爱玉及其传粉昆虫繁殖特性

薜荔(Ficus pumila L．var．pumila)隶属桑科榕属,爱玉(F．pumila L．var．awkeotsanmg Corner)为其变种,它们的花是单性的,雌雄异株。雌花序中着生雌花,雄花序中有瘿花和雄花,每个花序中花的数量极多,达4000～6000朵。薜荔榕小蜂是唯一能进入薜荔和爱玉的隐头花序中产卵或传粉的共生昆虫,自然状态下雌花的结实率分别为82％、83．52％;瘿花的成虫瘿率分别为58．71％、51．32％,因此可形成大量的果实和虫瘿。物候观察表明薜荔和爱玉花期不遇,它们花序中的榕小蜂种群已经生殖隔离。人为的放蜂实验表明,生活于爱玉花序中的榕小蜂,已无法在薜荔花序中繁殖,生殖隔离进一步得到证实;实验同时表明爱玉的花粉亦不能使薜荔雌花结实,宿主两变种间生理上已不亲和。本文从共生双方协同进化的角度出发,探讨了榕树2变种间与传粉昆虫繁殖特性的差异,以及变种产生的主要原因。     

Thiamine increases β-glucosidase production in the newly isolated strain of Fomitopsis pinicola

Aims:  To isolate a high β-glucosidase (BGL)-producing strain and to optimize BGL production in the isolated strain.
Methods and Results:  A high BGL-producing strain was isolated and identified as Fomitopsis pinicola KMJ812 based on its morphology and a comparison of sequence of its internal transcribed spacer rDNA gene. To increase BGL production, F. pinicola was supplemented with various vitamins. Supplementation with thiamine (20 mg l⁻¹) improved BGL production in F. pinicola cultures by 3·7-fold to give a specific activity of 114·4 μmol min⁻¹ mg⁻¹ protein, one of the highest among BGL-producing micro-organisms. The increased production of BGL in the thiamine-supplemented culture was confirmed by 2D electrophoresis followed by MS/MS sequencing. The BGL purified from F. pinicola culture showed the highest catalytic efficiency ever reported.
Conclusion:  Supplemental thiamine remarkably increased BGL production by a novel BGL-producing strain, F. pinicola KMJ812.
Significance and Impact of the Study:  Our results provide a high BGL-producing strain and the production media for BGL production, and should contribute to better industrial production of glucose via biological processes.     

红缘拟层孔菌化学成分与药理活性研究概述

对红缘拟层孔菌的化学成分、药理活性、菌丝发酵和木质素降解作用等方面的研究进行了综述。化学成分研究表明,红缘拟层孔菌含有多种三萜类、多糖类、脑苷脂类、挥发性等成分;药理活性研究表明,红缘拟层孔菌具有抗肿瘤、抗氧化、抗糖尿病、抗炎镇痛、抑菌、保肝等药理作用;菌丝发酵研究表明,红缘拟层孔菌菌丝发酵的最佳碳源为葡萄糖,氮源为酵母膏和麦芽汁。此外,红缘拟层孔菌对纤维素有降解作用。