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Sequence signals in eukaryotic upstream regions 总被引:3,自引:0,他引:3
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Structure of the arylsulfatase A gene 总被引:17,自引:0,他引:17
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Structural organization of the human CaMIII calmodulin gene 总被引:4,自引:0,他引:4
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An inspection of the domain between putative TATA box and translation start site in 79 plant genes. 总被引:95,自引:8,他引:87
C P Joshi 《Nucleic acids research》1987,15(16):6643-6653
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Characterisation of sequences required for RNA initiation from the PGK promoter of Saccharomyces cerevisiae. 总被引:4,自引:1,他引:3
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In the phosphoglycerate kinase (PGK) gene of yeast, as in other highly expressed yeast genes, the sequences surrounding the site of RNA initiation have a loosely conserved structure of a CT rich stretch followed by the tetranucleotide CAAG. Using internal deletions and insertions we have identified the elements in the PGK promoter which are required for correct RNA initiation at the CAAG sequence at -39. The results indicate that two different components of the PGK promoter contribute to correct RNA initiation, the TATA homologies, located at -152 and -113, and the sequences at the site of initiation. Both TATA elements can function in RNA initiation. Deletion of the upstream TATA element, TATAI, results in slightly heterogeneous RNA initiation, but the majority of the RNA initiates correctly. Deletion of both the PGK TATA elements results in the majority of the RNA initiating at sites downstream from the wild-type I site, within the structural gene between +40 to +80. The CT rich box is not essential for correct mRNA initiation as shown by deletion analysis. The site of RNA initiation in the PGK promoter appears to be determined by sequences located immediately 5' of the CAAG sequence motif. This short sequence, ACAGATC, when located the correct distance from the TATA elements may be sufficient to determine a discrete initiation site. 相似文献
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Sp1, a CAAT-binding factor, and the adenovirus major late promoter transcription factor interact with functional regions of the gamma-fibrinogen promoter. 总被引:12,自引:7,他引:5
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J G Morgan G Courtois G Fourel L A Chodosh L Campbell E Evans G R Crabtree 《Molecular and cellular biology》1988,8(6):2628-2637
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A 14 kilobase (kb) genomic clone of the gene for bovine elastin, containing exons 1 and 2, has been characterized. This clone extends about 6.5 kb in the 5' direction from the initiation codon and 978 nucleotides in the 3' direction from exon 2. The size of the first intron is about 6.4 kb. The sequence immediately 5' to the initiation codon is highly conserved between the genes for bovine and human elastins and contains a TATA box consensus sequence (ATAAA), CAAT, and Sp1 binding sites. Several putative AP-2 binding sites are also present. Comparative analysis of the sequences flanking the first exon in the genes for bovine and human elastins identified conserved sequences that may be regulatory control elements. A putative enhancer core sequence is present in the first intron of the genes for bovine and human elastins. 相似文献
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