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1.
To minimize the number of visits required to achieve seed-setfrom hand pollination whilst maintaining full control of thereproductive process in Eucalyptus globulus, we investigatedthe importance of style maturation on the progamic phase ofreproduction. Controlled pollinations were performed in: (1)fully receptive styles; (2) immature cut styles at or afterflower dehiscence; and (3) immature cut styles before flowerdehiscence. We show here that pollen germination and tube growthto the ovule occurred in all three treatments, and all producedviable seeds. Remarkably, tubes growing on immature sliced stylesof non-dehisced flowers reached the ovules by day 7, 12d faster than tubes growing on normal receptive stigmas. Moreover,more tubes entered the ovary in this kind of pollination and,consequently, more viable seeds were produced. We establishedhistochemically that tube growth in immature sliced styles isconcomitant with the presence of lipid secretion. These resultsfully support the efficiency of the so-called one-steppollination method in Eucalyptus globulus, which we nowprove can be applied more successfully to immature non-dehiscedflowers. General application of this procedure is likely tohave beneficial impacts on the planning and efficiency of large-scalegenetic improvement programs. Copyright 2001 Annals of BotanyCompany Eucalyptus globulus, one-step pollination, lipids, pollen, fertilization 相似文献
2.
The development of the pollen grain wall in Xiphidium coeruleum(Haemodoraceae) was studied using TEM and cytochemical stainingtechniques. Microsporocyte ontogeny initiates with the degradationof the cellulosic cell wall and subsequent deposition of a thickcallosic cell wall. Following callose deposition, successivemeiosis occurs, resulting in a tetragonal tetrad of microspores.during meiosis, the cell walls of the tapetum break down, releasingthe syncytial periplasmodium. Irregular non-sporopollenous globularbodies are deposited in this peripheral periplasmodium, whichis rich in ER, golgi bodies, vesicles, and characteristic starchplastids. Within the microspore cytoplasm, vesicles, golgi bodies,and plastids are plentiful during the early tetrad stage. Atthis time the plasma membrane of the microspore develops characteristicevaginations. An extracellular membrane, the white line,is secreted outside the microspore plasma membrane, followedby callose wall degradation. Bead-like deposits of exine orprimexine are deposited at points along the white linesimultaneously on inner and outer surfaces and opposite theoriginal plasma membrane evaginations. The bead-like exine depositscontinue to grow during the release of the microspores and developinto laterally appressed, rod-shaped ektexinous elements havinga tangentially oriented commissure, the vestige of the originalwhite line. The mature intine is two-layered,the outer exintine containing radially oriented vesicular structures,which are apparently derived from plasma membrane extensions.Exine development in Xiphidium is similar to nexine 1development in Lilium and may have evolved from an ancestraltectate-columellate condition by the loss of the sexine. Walldevelopment in members of the Zingiberales is strikingly similarto that reported here for the Haemodoraceaeevidence ofa possible relationship between the two taxa. Xiphidium coeruleum, Haemodoraceae, pollen, tapetum, development, exine 相似文献
3.
Development of seeds following pollination with irradiated pollenwas studied inActinidia deliciosa(kiwifruit) Hayward.Pollinations were carried out using two different sources ofpollen (Tomuri and Matua) irradiatedwith gamma rays at doses of 700 and 900 Gy. Non-irradiated crosseswere used as controls. Pollen irradiation had little effectonin vitropollen germination. Irradiated pollen affected seedset and seed content, and induced the formation of parthenogeneticembryos. In comparison to the control, the embryo growth ratewas slower and the endosperm contained very low amounts of storageproducts. Seed set was significantly reduced following bothdoses of irradiation. Two types of seeds were observed: (1)seeds with endosperm only; and (2) seeds with both embryo andendosperm. The proportion of seeds containing endosperm onlywas almost ten-fold higher than those containing both embryoand endosperm. Embryo production by gamma-irradiated pollenwas genotype- and dose-dependent. The induction of parthenogenesiswas higher following gamma ray doses of 900 Gy than 700 Gy,which suggests the Hertwig Effect; the best efficiencywas obtained with Tomuri pollen. Ploidy levelof parthenogenetic embryos was evaluated by nuclear size (area)with the use of image analysis. There was a large differencein embryo nuclei size between control and parthenogenetic embryos(mean size 90.8 and 49.1 µm2, respectively). It is concludedthat parthenogenetic embryos represent trihaploids.Copyright1998 Annals of Botany Company. Actinidia deliciosa, kiwifruit, pollen irradiation, induced parthenogenesis. 相似文献
4.
The Importance of Light Intensity for Pollen Tube Growth and Embryo Survival in Wheat x Maize Crosses 总被引:1,自引:0,他引:1
The success of Triticum aestivumxZea mays crosses, used to producewheat doubled haploids, is influenced by light intensity. Toexamine the basis for this response, pollen tube growth, embryosurvival and indicators of photosynthetic rate were measuredin two wheat cultivars (Karamu and Kotuku)crossed with maize at two irradiance levels (250 or 750 µmolm-2s-1, PAR). Pollen tube growth was significantly affectedby light intensity in Karamu plants but not inKotuku plants, despite both cultivars being pollinatedby the same maize source. The percentage of pollen tubes reachingthe cavity between the ovarian wall and integuments, or in themicropyle of Karamu plants at high light intensity(65%) was nearly three-times greater than that at low lightintensity (22%). Thus, either low light intensity can affectthe maternal wheat plant in a way that inhibits pollen tubegrowth and/or high light intensity may promote pollen tube growthin Karamu plants. Significant differences in ratesof electron transport in plants grown at the two light intensitiesindicated that the rate of photosynthesis may also have an effecton pollen tube growth. These results have importance for improvingthe efficiency of wheat x maize crosses and other wide cerealcrosses. Copyright 2001 Annals of Botany Company Intergeneric hybridization, light intensity, pollen tube growth, embryo survival, Triticum aestivum, wheat,Zea mays , maize 相似文献
5.
Dearnaley John D.W.; Levina Natalia N.; Lew Roger R.; Heath I. Brent; Goring Daphne R. 《Plant & cell physiology》1997,38(9):985-999
We have investigated Ca2+-involving cell signaling, plasma membranepotentials and conductances and callose formation during earlystages of pollination of papillae of Brassica napus. Using fluorescenceimaging of calcium green-1, we found that application of a rangeof pollen types and controls all rapidly produced small localizedpeaks in papillar cytoplasmic [Ca2+]. This response was morefrequent in compatible than incompatible interactions and wascorrelated with subsequent hydration of the applied pollen grains,indicating that it may be a differential prerequisite of thecompatible signaling pathway leading to successful pollinations.In contrast, a slight trend to increased plasma membrane conductance(but with no indications of action potential-like responses)and also callose deposition in papillae adjacent to pollen grainsfollowed pollination in both SC and SI interactions, indicatingthat alterations in plasma membrane permeability and callosedeposition during early phases of pollination are not primarydeterminants of the fate of attempted pollinations.
2On leave of absence from A.N. Bach Institute of Biochemistry,Russian Academy of Sciences, Leninsky Pr., 33, Moscow, Russia,117071 相似文献
6.
By means of hand pollination experiments and fluorescence microscopythe breeding systems and pollen tube growth were studied inDolichandra cynanchoides and Tabebuia nodosa, two bignoniaceousspecies of the Chaco woodland of NE Argentina. Both speciesabscised all selfed pistils within 48 d although selfpollen tubes grew successfully to the ovary and penetrated manyovules. Both species therefore show late-acting self-incompatibility(LSI) or ovarian sterility (OS) type control ofselfing. LSI-OS has been reported in 17 species of the Bignoniaceae,and is also clustered in families such as the Bombacaceae-Sterculiaceae,Fabaceae and Myrtaceae. The implications of such family clusteringare discussed, with regard to current hypotheses for the actionof LSI-OS phenomena. Copyright 1999 Annals of Botany Company Bignoniaceae, Dolichandra cynanchoides, Tabebuia nodosa, Chaco, late-acting self-incompatibility, ovarian sterility. 相似文献
7.
The Pollen-stigma Interaction: Pollen-tube Penetration in Crocus 总被引:6,自引:0,他引:6
In a compatible pollination in Crocus, pollen tube tips enterthe stigma papillae after the enzymic erosion of the cuticle,and the tubes continue downward growth towards the ovary betweenthe cuticle and the underlying pectocellulosic wall. The cuticleof the receptive zone of the stigma papilla is chambered, thechambers containing a secretion accumulated during the maturationof the stigma. Pollen exudates contain various acid hydrolases,but are incapable alone of eroding stigma cutin. Furthermore,there is no penetration when the proteins of the wall-held stigmasecretions are degraded enzymically. These facts are taken toindicate that the pollen contributes a cutinaseprecursor which is activated by a factor or factors held inthe stigma secretion. Pollens of certain Cruciferae producetubes capable of penetrating the Crocus stigma cuticle, suggestingthat notwithstanding the taxonomic remoteness of Cruciferaeand Iridaceae the enzyme activation systems are quite similar. 相似文献
8.
MARGINSON ROBYN; SEDGLEY MARGARET; KNOX R. BRUCE 《Journal of experimental botany》1985,36(10):1660-1668
Marginson, R., Sedgley, M. and Knox, R. B. 1985. Physiologyof post-pollination exudate production in AcaciaJ. exp.Bot. 36: 16601668. Stigmas of A. wattsiana produced 1260 nl of secretion by 90min after pollination. The optimum temperature for post-pollinationsecretion in A. baileyana, A. brownii and A. iteaphylla was20°C. Both self and cross intraspecific, interspecific andintergeneric pollinations produced a similar positive responsein A. iteaphylla and A. baileyana. In all cases aged pollenwas as effective as fresh pollen despite reduced fluorescencein the fluorescein diacetate test. Live yeast cells, fixed chickenerythrocytes, glass beads, talc and Biogel P150 did not stimulatesecretion, nor did pollen which had been washed in water orethanol despite high retention of viability in some cases Pollendiffusates dried on to glass beads produced a positive responsein A. iteaphylla and A. gracifolia. Intraspecific and interspecificpollinations involving fresh Acacia pollen resulted in pollengermination on the stigma and pollen tube growth in the styleof A. iteaphylla and A. baileyana. In contrast washed pollenfailed to germinate and pollen germination and tube growth werereduced at 35 °C as compared with 20 °C. Key words: Acacia, pollination, secretion 相似文献
9.
Pollen released at 1100 h has the highest viability (92.2%)but is no longer viable 3 d (84 h) after anthesis.In vitropollen-tubegrowth is fast (140 µm h-1) and increases significantlywithin the first 8 h.In vivopollen tubes also grow quickly andreach the base of the style within 2 h after pollination andenter the micropyle 8 h after pollination. There is no significantdifference between self- and cross-pollination in either therate and the number of pollen tubes in the pistil and the numberof ovules penetrated by a pollen tube. Teak has late-actinggametophytic self-incompatibility; the majority of pollen tubesgrow through the style but some do not continue to grow fromthe style towards the embryo sacs. Pollen-tube abnormalitiesinclude swollen, reversed, forked and tapered tips and irregularand spiralling tubes. These are most prevalent in self-pollination(20.4%). The index of self-incompatibility of 0.17 and low fruitset following self-pollination (2.49%) indicates that teak ismostly self-incompatible. Drastic fruit abortion occurs withinthe first week following controlled pollination. Within 14 d,fruit size and fruit set from cross-pollination is generallymuch greater than from self-pollination. Tectona grandis; pollen viability; pollen-tube growth; pollination; controlled pollinations; incompatibility. 相似文献
10.
The microfibrillar polysaccharide component of the pollen intinecan be isolated by progressive chemical digestion of the exineand the cellular contents and the extraction of the matrix materials.The resulting intine ghosts reveal various characteristicstructural features. The microfibrils have apparent individualdiameters in the range of 515 nm, but they are commonlyassociated laterally to form ribbons, or aggregated in strandsor cables of dimensions great enough to be resolved with theoptical microscope. These often show preferred orientations,which can be associated with pollen grain shape and with thedisposition-of the germination apertures. The apertural intinemay be structurally complex, as in Abutilon hybridum, where,after the removal of the exine, the polysaccharide caps whichoverlie the protein storage sites of the pollen grain wall retainthe elaborate patterning of the original cytoplasmic evaginationsfrom the vegetative cell. Secale cereale, Narcissus pseudonarcissus, Abutilon hybridum, Crocus vernus, pollen grain, intine, exine, wall pattern, germination apertures, polysaccharide microfibrils, fluorescence microscopy 相似文献
11.
《Journal of Molluscan Studies》1953,29(6):258
Part 1, under the frontispiece portrait of Dr. N. B. Eales,the words President 19481951 should havebeen added. Page 103, line 49, for Newton Collection readNorman Collection (Canon Norman). 185, line 37, for capillaris read capillacca. 188, Table 1, for bemoralis. read nemoralis. 188, Table 2, for Cochlicella acuta (Müll)? ventrosa(Fér.) read Cochlicella ventrosa (Fér.). 191, line 24, for araheo- read archeo-. 相似文献
12.
The effects of supplementary pollination on initial and finalnut set and nut weight of theMacadamia cultivars, HawaiiAgricultural Experiment Station 246 and HiddenValley A4, were assessed over 3 years at an orchard ineastern Australia. The final nut sets of racemes bagged to excludeinsect pollinators were low in all 3 years of the study forboth cultivars. This demonstrated the importance of exposureto insect visitors to increase nut set of these cultivars. However,the improved initial nut set by supplementary cross pollinationof 246 in all 3 years of the study showed that insect pollinationis inefficient compared to hand pollination. Furthermore, supplementarycross pollination of 246 increased final nut set by 5797%in 1989 and increased nut weight by 15.0% and kernel weightby 20.0% in 1991. Cross pollination of A4 did not result inany consistent pattern of initial nut set and did not improvefinal nut set, but increased nut weight by 11.6% and kernelweight by 18.4%, with a higher percentage kernel recovery in1991. In addition, supplementary self pollination of A4 increasednut sets in both 1989 and 1990. Yield and quality of both cultivarsmay benefit from increasing pollen transfer in the orchard. Macadamia integrifolia Maiden and Betche; Macadamia tetraphylla L. A. S. Johnson; macadamia; Proteaceae; cross pollination; self pollination; Apis mellifera ; Trigona ; nut set 相似文献
13.
Haploids (2n =24) of the common tetraploid (2n=48) potato (SolanumtuberosumL.) provide promising material for attacking many problemsconcerned with the genetics, cytogenetics and breeding of thisspecies. Interspecific 4xx2xcrosses betweenSolanum tuberosumgp.Andigenaorgp.Tuberosumcultivars as pistillate parents andSolanum tuberosumgp.Phurejaassource of pollen (hereafter pollinator) have beenused to produce maternally derived haploids through parthenogenesis.This paper discusses the nature of the pollinatoreffect in haploid extraction. The pollinator hada significant effect on haploid frequencies following 4xx2xcrosses.The pollinator effect seems to operate via theendosperm, in which haploid (n=2x) embryos are associated withhexaploid endosperm. A superior pollinator appearsto have its effect by contributing two haploid (n) gametes tothe central cell. 2n pollen; double fertilization; endosperm; ploidy manipulations; Solanum tuberosum 相似文献
14.
Pollen-wall Proteins: 'Gametophytic' and 'Sporophytic' Fractions in the Pollen Walls of the Malvaceae 总被引:4,自引:0,他引:4
Proteins are stored in two sites in the pollen grain walls ofthe Malvaceae, (a) in the cellulosic intine, mainly in the vicinityof the circular apertures, and (b) in cavities in the sculpturedlayer of the exine. The intine-held materials are incorporatedduring the growth of the wall. The exine materials are derivedfrom the tapetum, which during dissolution releases cistemaewith a granular-fibrillar content bounded by ribosomal membranes.This fraction is injected into the exine cavities after thecompletion of wall growth through micropores in the tectum.PAS-reacting material is associated with the injected protein.Another tapetal fraction, lipid in nature and commonly containingcarotenoids, remains on the surface of the pollen grains toform the Pollenkitt. While protein can be detected cytochemically in both intineand exine sites, acid phosphatase and ribonuclease activitywas found to be associated only with the former. Immunofluorescencemethods using antiserum to total pollen leachates showed thatantigens are present in both sites. When the pollen grains are moistened, the exine-held proteinsbegin to pass out through the micro-pores in the tectum within30 s of moistening, while the main discharge from the aperturalintine follows in 45 min. These observations, together with evidence from other families,suggest that the intine-held proteins of angiosperm pollen grainsare always produced by the male gametophyte, while those heldin exine cavities are sporophytic in origin, being derived fromthe tapetum. As previously proposed, it seems probable thatin intraspecific incompatibility systems of the gametophytictype control is mediated through intine-held recognitionsubstances, whereas in sporophytic systems the exine-heldmaterials are concerned. 相似文献
15.
Brassica plants, with a combination of chloroplasts encodingtriazine resistance and mitochondria encoding cytoplasmic malesterility, offer new opportunities for hybrid seed production.Such plants can be grown as a random mixture with a male parent,thereby allowing more effective pollen transfer for greaterefficiency of hybrid seed production. Harvested seed consistsof hybrid seed from the female parent, which istriazine-resistant, and non-hybrid, triazine-sensitive seedresulting from self pollination of the male parent. This study demonstrates that triazine-sensitive broccoli (BrassicaoleraceaL. var.italica) progeny can easily be eliminated byallowing the seed to imbibe a solution of 10 g l-1atrazine overnightthen drying-back the seed for subsequent germination. Such atreatment prevents triazine-sensitive broccoli from developingbeyond the cotyledon stage following germination, and remainseffective for at least 3.5 years after seed treatment. The growthof triazine-resistant broccoli plants is unaffected by thisseed treatment. Atrazine; Brassica oleracea; broccoli; hybrid seed; triazine resistance; seed treatment 相似文献
16.
A survey of pollen- and stigma-dimorphism and monomorphism inthe genus Limonium (as understood at present) reveals considerableheterogeneity which is useful for the solution of taxonomic,phylogenetic, and phytogeographic problems. Two main pollen types (A and B) and two main stigma types (coband papillate) occur in the genus. Capitate stigmata also occur. Type A pollen has frequently been found in conjunctionwith papillate stigmata as a secondarily monomorphic(self-compatible) combination and this must have arisen at leastfour times in the genus as well as in Armeria. Type Bpollen and cob stigmata are recorded togetherfor the first time (also as a secondarily monomorphic, self-compatiblecombination) in L. echioides. Apomixis, already discovered insubsections Densiflorae and Dissitiflorae, has been found inL. cosyrense of the subsection Steirocladae. The taxonomic and phylogenetic significance of the facts indiscussed. 相似文献
17.
Exploring the 'Most Effective Pollinator Principle' with Complex Flowers: Bumblebees and Ipomopsis aggregata 总被引:1,自引:0,他引:1
The most effective pollinator principle impliesthat floral characteristics often reflect adaptation to thepollinator that transfers the most pollen, through a combinationof high rate of visitation to flowers and effective depositionof pollen during each visit. We looked for the expected positivecorrelation between quantity and quality of visits in Ipomopsisaggregata, whose red, tubular flowers are considered to be adaptedto hummingbirds. Hummingbirds were indeed the most common floralvisitors in 5 years of observation. However, long-tongued bumblebeesdeposited on average three-times as much outcross pollen pervisit to virgin flowers, and elicited four-times as much seedproduction, as did hummingbirds. Hence visitors that are relativelyinfrequent, and unexpected given the pollination syndromeof the plant, can be surprisingly good pollinators. One interpretationof this observation is that natural selection favours a specializedfloral morphology that excludes all but a single type of visitor,but that there are constraints on achieving this outcome. Analternative is that selection favours some degree of floralgeneralization, but that flowers can retain features that adaptthem to a particular type of pollinator in spite of this generalization.Copyright 2001 Annals of Botany Company Bumblebees, floral evolution, hummingbirds, Ipomopsis aggregata, pollination syndromes, pollinator effectiveness, scarlet gilia, seed set, specialization, temporal variation 相似文献
18.
Reproduction in Seagrasses: Nature of the Pollen and Receptive Surface of the Stigma in the Hydrocharitaceae 总被引:1,自引:0,他引:1
This paper describes the characteristics of the pollen and thereceptive surfaces of the stigmas in the three marine angiospermsincluded in the Hydrocharitaceae. The pollen in Enhalus acoroidesand Thalassia hemprichii is spherical and has an ornamentedexine. An exine layer is not found in Halophila stipulacea wherereniform pollen grains are contained within transparent moniliformtubes. Cytochemical tests show that the pollen wall in the threespecies contains acidic and neutral polysaccharides and acidhydrolase acitivity is detected in the intine of H. stipulaceaand T. hemprichii. In Thalassia, one of the intine enzymes,acid phosphatase, is unambiguously associated with cytoplasmicinclusions. Flowering in Thalassia is coincident with the spring tides andthe pollen is released as a mass suspended in a thecal slimewhich contains approximately 5 per cent by weight carbohydrate,the principal mono-saccharide being mannose. Electrophoreticanalysis of the pollen-free slime shows a single glycoproteincomponent. The stigmas of the three seagrasses are papillate and of thedry type possessing a continuous protein-aceouspellicle subtended by a cuticle. The stigma pellicle exhibitscytochemically detectable esterase activity and binds the lectinconcanavalin A. Acid phosphatase activity is localized beneaththe cuticle at the tips of the stigma papillae. The discoveries show that the characteristics of the pollenand stigmas in the seagrasses are comparable with those foundin terrestrial flowering plants. The similarity in enzymaticproperites of the pollen wall and stigma pellicle suggests that,intriguingly, a similar mechanism of cuticle erosion might wellfollow compatible pollination both on land and in the sea. Enhalus acoroides, Thalassia hemprichii, Halophila stipulacea, Halophila decipiens, seagrasses pollen wall, stigma surface, hydrolytic enzymes 相似文献
19.
The various pathways of pollen development were investigatedin cultured anthers of Lolium temulentum, Festuca pratensisand the L. multiflorum x F. pratensis hybrid Elmet.In all three, development from the vegetative cell was the predominantpathway of pollen callus development. However, there were characteristicdifferences in the behaviour of the generative cell. In L. temulentumit remained attached to the pollen wall and degenerated, whereasin F. pratensis it divided. In Elmet it detachedfrom the pollen wall and remained undivided. Both polarizedand unpolarized partitioned calluses were observed. Developmentof the fusion product of the vegetative and generative nucleiwere also observed in anthers of L. temulentum. Anomalous grainswere not found to be major source of pollen calluses. Sections of anthers of L. temulentum were used to investigatethe origin of S pollen grains, the small pale-staining grainswhich denote pollen dimorphism. Such grains form out of contactwith the tapetum and are therefore determined before or duringmeiosis (i.e. before harvest of anthers for culture). Sectionswere also used to demonstrate the influence of the durationof pretreatment on the development of the middle layer of theanther wall. Festuca pratensis, Lolium temulentum, Lolium x Festuca, anther culture, haploid, microspore, pollen 相似文献
20.
- A close parallelism in the drift of the rate of respirationand the protein-N/ non-protein-N ratio is shown to occur bothin apple fruits attached to the tree and when detached fromthe tree at various stages of development and stored for severalmonths at 12 C.
- In detached fruits the fall in respirationwhich occurs immediately(during the first 48 hours) after pickingis only accompaniedby a concomitant fall in net protein invery young fruits inwhich active cell division is taking place.Subsequently, infruit of all ages when a climacteric rise inrespiration occursit is accompanied by a net increase in protein.
- It is argued that the climacteric rise in respiration is aresultof increase in the level of protein which will be expectedtoreduce the ATP/ADP ratio.
- Over the climacteric period,although rate of respiration andnet protein content both rise,R rises more rapidly than proteinand, subsequently, falls ata faster rate than P. It is suggestedthat this may be due tothe new protein containinga higher proportionof enzyme(s) directly involved in respirationand leading, forexample, to a reduction in the ATP/ADP ratio.