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Objective : Determine the biochemical pathways involved in induction of apoptosis by ajoene, an organosulfur compound from garlic. Research Methods and Procedures : Mature 3T3‐L1 adipocytes were incubated with ajoene at concentrations up to 200 μM. Viability and apoptosis were quantified using an MTS‐based cell viability assay and an enzyme‐linked immunosorbent assay for single‐stranded DNA (ssDNA), respectively. Intracellular reactive oxygen species (ROS) production was measured based on production of the fluorescent dye, dichlorofluorescein. Activation of the mitogen‐activated protein kinases extracellular signal‐regulating kinase 1/2 (ERK) and c‐Jun‐N‐terminal kinase (JNK) was shown by Western blot. Western blot was also used to show activation of caspase‐3, translocation of apoptosis‐inducing factor (AIF) from mitochondria to nucleus, and cleavage of 116‐kDa poly(ADP‐ribose) polymerase (PARP)‐1. Results : Ajoene induced apoptosis of 3T3‐L1 adipocytes in a dose‐ and time‐dependent manner. Ajoene treatment resulted in activation of JNK and ERK, translocation of AIF from mitochondria to nucleus, and cleavage of 116‐kDa PARP‐1 in a caspase‐independent manner. Ajoene treatment also induced an increase in intracellular ROS level. Furthermore, the antioxidant N‐acetyl‐l ‐cysteine effectively blocked ajoene‐mediated ROS generation, activation of JNK and ERK, translocation of AIF, and degradation of PARP‐1. Discussion : These results indicate that ajoene‐induced apoptosis in 3T3‐L1 adipocytes is initiated by the generation of hydrogen peroxide, which leads to activation of mitogen‐activated protein kinases, degradation of PARP‐1, translocation of AIF, and fragmentation of DNA. Ajoene can, thus, influence the regulation of fat cell number through the induction of apoptosis and may be a new therapeutic agent for the treatment of obesity. 相似文献
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Wasting syndrome is one of the hallmark symptoms of poisoning by TCDD (=dioxin), which is associated with the massive loss of adipose tissue and serum hyperlipidemia in vivo. Yet, the most widely used in vitro cell model 3T3-L1 adipocyte has not been useful for studying such an action of TCDD because of the difficulty of inducing their mature adipocytes to respond to TCDD to go through lipolysis. Here, we made efforts to find the right cell culture and treatment conditions to induce mature 3T3-L1 adipocytes to go through lipolysis, which is defined as events leading to reduction of lipids in adipocytes. The optimum condition was found to require 7-day differentiated adipocytes being subjected to DMEM medium containing TCDD (but without insulin) for 5 day incubation with two medium changes (the same composition) on incubation days 2 and 4. After 24 h, the early effect of TCDD on adipocytes was predominantly on inflammation, particularly induction of COX-2 and KC (IL-8), which is accompanied by upregulation of C/EBPbeta and delta. The sign of TCDD-induced lipolysis starts slowly and by incubation day 3, a few markers showed modestly significant changes. By day 5 of incubation, however, many markers show highly significant signs of lipolytic changes. Although this process could take place without exogenous macrophages or their cytokines, addition of exogenous TNFalpha considerably synergized this action of TCDD. In conclusion, under a right condition, 3T3-L1 adipocytes were found to respond to TCDD to go through lipolysis. The early trigger of such a response appears to be activation of COX-2, which is amplified by TNFalpha. 相似文献
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Su‐Jin Park Jae Ho Park Anna Han Munkhtugs Davaatseren Hyun Jin Kim Myung‐Sunny Kim Haeng Jeon Hur Mi‐Jeong Sung Jin‐Taek Hwang Hye Jeong Yang Dae Young Kwon 《Cell biochemistry and function》2015,33(4):220-225
The purpose of this study is to investigate the effects of euphorbiasteroid, a component of Euphorbia lathyris L., on adipogenesis of 3T3‐L1 pre‐adipocytes and its underlying mechanisms. Euphorbiasteroid decreased differentiation of 3T3‐L1 cells via reduction of intracellular triglyceride (TG) accumulation at concentrations of 25 and 50 μM. In addition, euphorbiasteroid altered the key regulator proteins of adipogenesis in the early stage of adipocyte differentiation by increasing the phosphorylation of AMP‐activated protein kinase (AMPK) and acetyl‐CoA carboxylase. Subsequently, levels of adipogenic proteins, including fatty acid synthase, peroxisome proliferator‐activated receptor‐γ and CCAAT/enhancer‐binding protein α, were decreased by euphorbiasteroid treatment at the late stage of adipocyte differentiation. The anti‐adipogenic effect of euphorbiasteroid may be derived from inhibition of early stage of adipocyte differentiation. Taken together, euphorbiasteroid inhibits adipogenesis of 3T3‐L1 cells through activation of the AMPK pathway. Therefore, euphorbiasteroid and its source plant, E. lathyris L., could possibly be one of the fascinating anti‐obesity agent. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
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Atsuko Kitahara Kazuto Takahashi Rie Moriya Hirohisa Onuma Keiko Handa Yoshikazu Sumitani Toshiaki Tanaka Hidenori Katsuta Susumu Nishida Takuya Sakurai Kouichi Inukai Hideki Ohno Hitoshi Ishida 《Journal of cellular physiology》2015,230(1):199-209
Ghrelin is a physiological‐active peptide with growth hormone‐releasing activity, orexigenic activity, etc. In addition, the recent study has also suggested that ghrelin possesses the pathophysiological abilities related with type 2 diabetes. However, the ghrelin‐direct‐effects implicated in type 2 diabetes on peripheral tissues have been still unclear, whereas its actions on the central nervous system (CNS) appear to induce the development of diabetes. Thus, to assess its peripheral effects correlated with diabetes, we investigated the regulatory mechanisms about adipokines, which play a central role in inducing peripheral insulin resistance, secreted from mature 3T3‐L1 adipocytes stimulated with ghrelin in vitro . The stimulation with 50 nmol/L ghrelin for 24 h resulted in the significant 1.9‐fold increase on vascular endothelial growth factor‐120 (VEGF120) releases (p < 0.01) and the 1.7‐fold on monocyte chemoattractant protein‐1 (MCP‐1) (p < 0.01) from 3T3‐L1 adipocytes, respectively, while ghrelin failed to enhance tumor necrosis factor‐α (TNF‐α), interleukin‐1β (IL‐1β), IL‐6, IL‐10 and adiponectin secretions. In addition, Akt phosphorylation on Ser473 and c‐Jun NH2‐terminal protein kinase (JNK) phosphorylation on Thr183/Tyr185 were markedly enhanced 1.4‐fold (p < 0.01) and 1.6‐fold (p < 0.01) in the ghrelin‐stimulated adipocytes, respectively. Furthermore, the treatment with LY294002 (50 μmol/L) and Wortmannin (10nmol/L), inhibitors of phosphatidylinositol 3‐kinase (PI3K), significantly decreased the amplified VEGF120 secretion by 29% (p < 0.01) and 28% (p < 0.01) relative to the cells stimulated by ghrelin alone, respectively, whereas these inhibitors had no effects on increased MCP‐1 release. On the other hand, JNK inhibitor SP600125 (10 μmol/L) clearly reduced the increased MCP‐1, but not VEGF120, release by 35% relative to the only ghrelin‐stimulated cells (p < 0.01). In conclusion, ghrelin can enhance the secretions of proinflammatory adipokines, VEGF120 and MCP‐1, but fails to affect IL‐10 and adiponectin which are considered to be anti‐inflammatory adipokines. Moreover, this augmented VEGF120 release is invited through the activation of PI3K pathways and the MCP‐1 is through JNK pathways. Consequently, our results strongly suggest that ghrelin can induce the development of diabetes via its direct‐action in peripheral tissues as well as via in CNS. J. Cell. Physiol. 230: 199–209, 2015. © 2014 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals, Inc. 相似文献
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The high incidence of obesity-related pathologies, led to the study of the mechanisms involved in preadipose cell proliferation and differentiation. Here, we demonstrate that modulation of erbB2, plays a fundamental role during proliferation and adipogenic induction of preadipocytes. Using 3T3-L1 cells as model, we demonstrate that EGF (10 nM, 5 min) in addition to stimulate receptor tyrosine phosphorylation of both erbB2 and EGFR, is able to induce the heterodimer erbB2-EGFR. We treated proliferating 3T3-L1 cells with two inhibitors, AG 825 (IC(50) 0.35 microM, 54 times more selective for erbB2 than for EGFR, IC(50) 19 microM), and AG 879 (IC(50) of 1 microM for erbB2 versus 500 microM for EGFR). We found that both inhibited the proliferation on a dose-dependent basis, reaching a 30% maximal inhibition at 100 microM (P < 0.001) for AG825, and a 20% maximal inhibition at 10 microM (P < 0.001) for AG 879. These results involve erbB2 in 3T3-L1 proliferation. When studying the differentiation process, we found that the action of MIX-Dexa immediately activates MEK, JNK and p38 kinases. We observed that PD98059 and SP600125 (MEK-ERK and JNK inhibitors, respectively) added 1 h prior to the MIX-Dexa induction produced a decrease in erbB2 expression after 6 h, which is even greater than the one produced by the inducers, MIX-Dexa. This work supports erbB2 as a key factor in 3T3-L1 adipogenesis, acting mostly and not only during the proliferative phase but also during the differentiation through modulation of both its expression and activity. 相似文献
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Recent epidemiologic studies pointed out a significant correlation between dietary monosodium glutamate (MSG) and increased body mass index. Corroborating evidences came from animal studies depicting a clear association between dietary MSG intake and increased abdominal fat, dyslipidemia, adipocyte hypertrophy, and total body weight gain. Taken together with the inferred absence of conspicuous hypothalamic neuropathies the hallmark of disease etiopathogenesis in MSG‐obese animals, these animal studies with dietary MSG strongly argue for the presence of an alternative non‐neuronal route for MSG to mediate its adipose tissue‐specific phenotype and body weight gain. On the basis of this hypothesis, we investigated the direct effect of physiologically relevant low (100 µM), moderate (250 µM), and high dosages (2.5 and 25 mM) of MSG on distinct phases of adipocyte differentiation. MSG‐dependent changes in cell proliferation and lipid accumulation were analyzed by cell proliferation assays, flow cytometry, and biochemical methods, respectively. Physiologically relevant high dosages MSG demonstrated a significant potential in reducing MCE and thereof adipogenic capacity of preadipocytes in a dose‐dependent manner by restricting the availability of critical mitogenic proteins, CCAAT/enhancer‐binding protein β (CEBPβ), and the mitotic cyclin B. Our findings warrant further investigations to unravel the effect of long‐term dietary MSG intake on capacity of preadipocytes in different fat depots to undergo mitotic clonal expansion and hyperplasia in rodent models and human subjects, respectively. 相似文献
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Biogenesis,characterization, and the effect of vicenin‐gold nanoparticles on glucose utilization in 3T3‐L1 adipocytes: A bioinformatic approach to illuminate its interaction with PTP 1B and AMPK 
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下载免费PDF全文 Shivashri Chockalingam Rajarajeshwari Thada Ramesh Kumar Dhandapani Rajasekar Panchamoorthy 《Biotechnology progress》2015,31(4):1096-1106
This study reported the synthesis of Vicenin‐2 gold nanoparticles (VN‐AuNPs) and evaluated their effect on the glucose utilization efficiency of 3T3‐L1 adipocytes. The VN‐AuNPs were characterized by microscopic, DLS and spectral analysis. The bio‐reducing efficiency of Vicenin‐2 (VN) was computed and confirmed by HPLC analysis. The stability of VN‐AuNPs in various physiological media was explored. The cytotoxicity and glucose uptake assays were performed in 3T3‐L1 adipocytes. The docking of VN with PTP1B and AMPK was also performed. The color change and UV absorption at 537 nm preliminarily confirmed the VN reduced gold nanoparticles. The VN‐AuNPs appeared as spherical particles (57 nm) and face centered cubic crystals under TEM and XRD analysis, respectively. Its zeta potential was found to be ?6.53 mV. The FT‐IR spectra of VN and its AuNPs confirmed its stability. The computed reducing potential of VN was similar to the extent of VN utilized during the synthesis of VN‐AuNPs. The VN‐AuNPs showed a remarkable stability in different physiological media. At 100 µM concentration, VN‐AuNPs displayed 78.21% cell viability. A concentration dependent increase in glucose uptake was noted in 3T3‐L1 adipocytes when incubated with VN‐AuNPs. The docking data revealed a strong interaction of VN with the binding pockets of PTP1B and AMPK. This demonstrates that the fabricated VN‐AuNPs might enhance the intracellular VN availability mediated cellular glucose utilization and this would serve as a novel nanodrug for the management of diabetes. © 2015 American Institute of Chemical Engineers Biotechnol. Prog., 31:1096–1106, 2015 相似文献
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Zhu Zhu Lirong Xu Tingting Cai Gongsheng Yuan Ning Sun Chao Lu Ruizhe Qian 《Journal of cellular physiology》2018,233(8):6028-6040
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Ok‐Hwan Lee Kye‐Yoon Yoon Kui‐Jin Kim SangGuan You Boo‐Yong Lee 《Journal of phycology》2011,47(3):548-556
Recent studies suggest that seaweed extracts are a significant source of bioactive compounds comparable to the dietary phytochemicals such as onion and tea extracts. The exploration of natural antioxidants that attenuate oxidative damage is important for developing strategies to treat obesity‐related pathologies. The objective of this study was to screen the effects of seaweed extracts of 49 species on adipocyte differentiation and reactive oxygen species (ROS) production during the adipogenesis in 3T3‐L1 adipocytes, and to investigate their total phenol contents and 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging activities. Our results show that high total phenol contents were observed in the extracts of Ecklonia cava (see Table 1 for taxonomic authors) (681.1 ± 16.0 μg gallic acid equivalents [GAE] · g?1), Dictyopteris undulata (641.3 ± 70.7 μg GAE · g?1), and Laurencia intermedia (560.9 ± 48.1 μg GAE · g?1). In addition, DPPH radical scavenging activities were markedly higher in Sargassum macrocarpum (60.2%), Polysiphonia morrowii (55.0%), and Ishige okamurae (52.9%) than those of other seaweed extracts (P < 0.05). Moreover, treatment with several seaweed extracts including D. undulata, Sargassum micracanthum, Chondrus ocellatus, Gelidium amansii, Gracilaria verrucosa, and Grateloupia lanceolata significantly inhibited adipocyte differentiation and ROS production during differentiation of 3T3‐L1 preadipocytes. Furthermore, the production of ROS was positively correlated with lipid accumulation (R2 = 0.8149). According to these preliminary results, some of the seaweed extracts can inhibit ROS generation, which may protect against oxidative stress that is linked to obesity. Further studies are required to determine the molecular mechanism between the verified seaweeds and ROS, and the resulting effects on obesity. Table 1. List of Korean seaweed extracts of 49 species evaluated in this experiment.
| Type | No. | Scientific name | Collection time | TP1 (μg GAE · g?1) |
|---|---|---|---|---|
| Brown macroalgae | SE‐1 | Chondracanthus tenellus (Harv.) Hommers. | April 27, 2006 | 112.8 ± 15.1lm |
| SE‐2 | Colpomenia sinusa (F. C. Mertens ex Roth) Derbes et Solier in Castagne | May 11, 2006 | 44.0 ± 4.1opqrs | |
| SE‐3 | Dictyopteris divaricata (Okamura) Okamura | April 6, 2006 | 41.5 ± 5.6pqrs | |
| SE‐4 | Dictyopteris pacifica (Yendo) I. K. Hwang, H.‐S. Kim et W. J. Lee | April 27, 2006 | 80.9 ± 8.3mno | |
| SE‐5 | Dictyopteris prolifera (Okamura) Okamura | November 26, 2007 | 48.4 ± 3.0nopqrs | |
| SE‐6 | Dictyopteris undulata Holmes | July 28, 2007 | 641.3 ± 70.7b | |
| SE‐7 | Dictyota asiatica I. K. Hwang | April 6, 2006 | 52.9 ± 7.6nonopqr | |
| SE‐8 | Ecklonia cava Kjellm. | October 22, 2006 | 681.1 ± 16.0a | |
| SE‐9 | Ecklonia stolonifera Okamura | November 26, 2007 | 36.5 ± 3.4pqrs | |
| SE‐10 | Endarachne binghamiae J. Agardh | March 10, 2006 | 50.4 ± 2.6nopqrs | |
| SE‐11 | Hizikia fusiformis (Harv.) Okamura | July 23, 2006 | 16.4 ± 1.2rs | |
| SE‐12 | Hydroclathrus clathratus (C. Agardh) M. Howe | May 11, 2006 | 18.1 ± 0.9rs | |
| SE‐13 | Ishige okamurae Yendo | May 26, 2006 | 237.4 ± 1.6h | |
| SE‐14 | Lethesia difformis (L.) Aresch. | May 11, 2006 | 11.2 ± 1.9s | |
| SE‐15 | Myelophycus simplex (Harv.) Papenf. | April 27, 2006 | 39.5 ± 3.2pqrs | |
| SE‐16 | Padina arborescens Holmes | July 29, 2007 | 172.9 ± 23.1ij | |
| SE‐17 | Sargassum fulvellum (Turner) C. Agardh | April 27, 2006 | 119.1 ± 5.6kl | |
| SE‐18 | Sargassum micracanthum (Kütz.) Endl. | December 21, 2006 | 468.0 ± 22.7e | |
| SE‐19 | Sargassum patens C. Agardh | January 21, 2007 | 41.5 ± 5.7pqrs | |
| SE‐20 | Sargassum confusum C. Agardh f. validum Yendo | March 8, 2008 | 110.9 ± 3.5lm | |
| SE‐21 | Sargassum horneri (Turner) C. Agardh | March 1, 2006 | 84.8 ± 9.4lmn | |
| SE‐22 | Sargassum macrocarpum C. Agardh | January 21, 2007 | 353.9 ± 59.1g | |
| SE‐23 | Sargassum muticum (Yendo) Fensolt | January 21, 2007 | 72.1 ± 14.9nop | |
| SE‐24 | Sargassum nipponium Yendo | April 6, 2006 | 54.0 ± 3.5nopqr | |
| SE‐25 | Sargassum sagamianum Yendo | March 8, 2008 | 41.0 ± 6.7pqrs | |
| SE‐26 | Sargassum thunbergii (Mertens ex Roth) Kuntze | July 23, 2006 | 27.7 ± 0.8qrs | |
| SE‐27 | Scytosiphon gracilis Kogame | May 26, 2006 | 30.2 ± 5.6qrs | |
| SE‐28 | Scytosiphon lomentaria (Lyngb.) Link | May 11, 2006 | 66.5 ± 8.9nopq | |
| Red macroalgae | SE‐29 | Bonnemaisonia hamifera Har. | April 27, 2006 | 44.1 ± 2.3opqrs |
| SE‐30 | Callophyllis crispata Okamura | May 11, 2006 | 37.6 ± 12.6pqrs | |
| SE‐31 | Chondria crassicaulis Harv. | May 11, 2006 | 45.4 ± 4.4opqrs | |
| SE‐32 | Chondrus crispus Stackh. | May 26, 2006 | 40.7 ± 8.0pqrs | |
| SE‐33 | Chondrus ocellatus Holmes | May 11, 2006 | 47.2 ± 1.7nopqrs | |
| SE‐34 | Gelidium amansii (J. V. Lamour.) J. V. Lamour. | April 27, 2006 | 525.3 ± 35.9d | |
| SE‐35 | Gloioperltis furcata (Postels et Rupr.) J. Agardh | May 26, 2006 | 147.7 ± 6.4jk | |
| SE‐36 | Gloioperltis complanta (Harv.) Yamada | May 26, 2006 | 58.2 ± 6.4nopq | |
| SE‐37 | Gracilaria verrucosa (Hudson) Papenf. | March 6, 2008 | 55.1 ± 7.5nopqr | |
| SE‐38 | Grateloupia elliptica Holmes | May 26, 2006 | 154.4 ± 12.9j | |
| SE‐39 | Grateloupia filicina (J. V. Lamour.) C. Agardh | May 11, 2006 | 38.2 ± 2.2pqrs | |
| SE‐40 | Grateloupia lanceolata (Okamura) Kawag. | July 23, 2006 | 32.7 ± 3.0pqrs | |
| SE‐41 | Laurencia intermedia J. V. Lamour. | May 11, 2006 | 560.9 ± 48.1c | |
| SE‐42 | Laurencia intricata J. V. Lamour. | April 27, 2006 | 35.4 ± 4.0pqrs | |
| SE‐43 | Laurencia okamurae Yamada | May 11, 2006 | 193.2 ± 41.9i | |
| SE‐44 | Lomentaria hakodatensis Yendo | April 27, 2006 | 165.2 ± 15.1ij | |
| SE‐45 | Polyopes affinis (Harv.) Kawag. et H.‐W. Wang | May 26, 2006 | 42.9 ± 2.3opqrs | |
| SE‐46 | Polysiphonia morrowii Harv. | May 11, 2006 | 392.4 ± 40.3f | |
| SE‐47 | Prionitis cornea (Okamura) E. Y. Dawson | October 22, 2006 | 47.9 ± 3.6nopqrs | |
| Green macroalgae | SE‐48 | Enteromorpha prolifera (O. F. Müll.) J. Agardh | March 26, 2006 | 42.0 ± 5.3pqrs |
| SE‐49 | Ulva pertusa Kjellm. | April 27, 2006 | 48.3 ± 3.8nopqrs |
- GAE, gallic acid equivalents; SE, seaweed extracts.
- 1TP, total phenol content is micrograms of total phenol contents per gram of seaweed extract based on gallic acid as standard. The values are means ± SD from three replications.
- a–sMeans in the same column not sharing a common letter are significantly different (P < 0.05) by Duncan’s multiple test.
Citing Literature
Number of times cited according to CrossRef: 21
- Kas?m Cemal Güven, Burak Coban, Osman Özdemir, Pharmacology of Marine Macroalgae, Encyclopedia of Marine Biotechnology, 10.1002/9781119143802, (585-615), (2020). Wiley Online Library
- Giovanna Bermano, Teodora Stoyanova, Franck Hennequart, Cherry L. Wainwright, Seaweed-derived bioactives as potential energy regulators in obesity and type 2 diabetes, , 10.1016/bs.apha.2019.10.002, (2019). Crossref
- Ana Rocío Múzquiz de la Garza, Mireya Tapia-Salazar, Maribel Maldonado-Muñiz, Julián de la Rosa-Millán, Janet Alejandra Gutiérrez-Uribe, Liliana Santos-Zea, Bertha Alicia Barba-Dávila, Denis Ricque-Marie, Lucía Elizabeth Cruz-Suárez, Nutraceutical Potential of Five Mexican Brown Seaweeds, BioMed Research International, 10.1155/2019/3795160, 2019 , (1-15), (2019). Crossref
- M. Lynn Cornish, Alan T. Critchley, Ole G. Mouritsen, A role for dietary macroalgae in the amelioration of certain risk factors associated with cardiovascular disease, Phycologia, 10.2216/15-77.1, 54 , 6, (649-666), (2019). Crossref
- Carolina Gonçalves-Fernández, Jorge Sineiro, Ramón Moreira, Oreste Gualillo, Extraction and characterization of phlorotannin-enriched fractions from the Atlantic seaweed Bifurcaria bifurcata and evaluation of their cytotoxic activity in murine cell line, Journal of Applied Phycology, 10.1007/s10811-018-1729-2, (2019). Crossref
- Noelia Flórez‐Fernández, María P Casas, María Jesús González‐Muñoz, Herminia Domínguez, Microwave hydrogravity pretreatment of Sargassum muticum before solvent extraction of antioxidant and antiobesity compounds, Journal of Chemical Technology & Biotechnology, 10.1002/jctb.5771, 94 , 1, (256-264), (2018). Wiley Online Library
- Yannick Lerat, M. L. Cornish, Alan T. Critchley, Stéphane La Barre, Stephen S. Bates, Applications of Algal Biomass in Global Food and Feed Markets: From Traditional Usage to the Potential for Functional Products, Blue Biotechnology, 10.1002/9783527801718, (143-189), (2018). Wiley Online Library
- Gabriele Andressa Zatelli, Ana Cláudia Philippus, Miriam Falkenberg, An overview of odoriferous marine seaweeds of the Dictyopteris genus: insights into their chemical diversity, biological potential and ecological roles, Revista Brasileira de Farmacognosia, 10.1016/j.bjp.2018.01.005, 28 , 2, (243-260), (2018). Crossref
- Cyr Abel Maranguy Ogandaga, Yeon Ju Na, Sang-Rae Lee, Young Sik Kim, Han Gil Choi, Ki Wan Nam, Wart-like spot formation on the fronds of Chondrus ocellatus (Gigartinales) by a brown alga, Mikrosyphar zosterae (Ectocarpales) in Korea, Journal of Applied Phycology, 10.1007/s10811-016-1028-8, 29 , 5, (2539-2546), (2017). Crossref
- Fook Yee Chye, Birdie Scott Padam, Seah Young Ng, Innovation and Sustainable Utilization of Seaweeds as Health Foods, Sustainability Challenges in the Agrofood Sector, 10.1002/9781119072737, (390-434), (2017). Wiley Online Library
- Gaurav Rajauria, Lynn Cornish, Francesco Ometto, Flower E. Msuya, Raffaella Villa, Identification and selection of algae for food, feed, and fuel applications, Seaweed Sustainability, 10.1016/B978-0-12-418697-2.00012-X, (315-345), (2015). Crossref
- Jatinder Sangha, Owen Wally, Arjun Banskota, Roumiana Stefanova, Jeff Hafting, Alan Critchley, Balakrishnan Prithiviraj, A Cultivated Form of a Red Seaweed (Chondrus crispus), Suppresses β-Amyloid-Induced Paralysis in Caenorhabditis elegans, Marine Drugs, 10.3390/md13106407, 13 , 10, (6407-6424), (2015). Crossref
- Jung-Ae Kim, Fatih Karadeniz, Byul-Nim Ahn, Myeong Sook Kwon, Ok-Ju Mun, Mihyang Kim, Sang-Hyeon Lee, Ki Hwan Yu, Yuck Yong Kim, Chang-Suk Kong, Sargassum sp. Attenuates Oxidative Stress and Suppresses Lipid Accumulation in vitro, Journal of Life Science, 10.5352/JLS.2014.24.3.274, 24 , 3, (274-283), (2014). Crossref
- Georgia M. Hart, Tamara Ticktin, Dovi Kelman, Anthony D. Wright, Nicole Tabandera, Contemporary Gathering Practice and Antioxidant Benefit of Wild Seaweeds in Hawai’i, Economic Botany, 10.1007/s12231-014-9258-7, 68 , 1, (30-43), (2014). Crossref
- Zahid Manzoor, Vivek Bhakta Mathema, Doobyeong Chae, Eun-Sook Yoo, Hee-Kyoung Kang, Jin-Won Hyun, Nam Ho Lee, Mi-Hee Ko, Young-Sang Koh, Extracts of the seaweed Sargassum macrocarpum inhibit the CpG-induced inflammatory response by attenuating the NF-κB pathway, Food Science and Biotechnology, 10.1007/s10068-014-0041-4, 23 , 1, (293-297), (2013). Crossref
- Jatinder Singh Sangha, Di Fan, Arjun H. Banskota, Roumiana Stefanova, Wajahatullah Khan, Jeff Hafting, James Craigie, Alan T. Critchley, Balakrishnan Prithiviraj, Bioactive components of the edible strain of red alga, Chondrus crispus, enhance oxidative stress tolerance in Caenorhabditis elegans, Journal of Functional Foods, 10.1016/j.jff.2013.04.001, 5 , 3, (1180-1190), (2013). Crossref
- Areum Daseul Kim, Mei Jing Piao, Yu Jae Hyun, Hee Kyoung Kang, In Soo Suh, Nam Ho Lee, Jin Won Hyun, Photo-protective properties of Lomentaria hakodatensis yendo against ultraviolet B radiation-induced keratinocyte damage, Biotechnology and Bioprocess Engineering, 10.1007/s12257-012-0336-3, 17 , 6, (1223-1231), (2013). Crossref
- Min‐Jung Seo, Hyeon‐Son Choi, Ok‐Hwan Lee, Boo‐Yong Lee, Grateloupia lanceolata (Okamura) Kawaguchi, the Edible Red Seaweed, Inhibits Lipid Accumulation and Reactive Oxygen Species Production During Differentiation in 3T3‐L1 Cells, Phytotherapy Research, 10.1002/ptr.4765, 27 , 5, (655-663), (2012). Wiley Online Library
- Mi‐Seon Woo, Hyeon‐Son Choi, Ok‐Hwan Lee, Boo‐Yong Lee, The Edible red Alga, Gracilaria verrucosa, Inhibits Lipid Accumulation and ROS Production, but Improves Glucose Uptake in 3T3‐L1 Cells, Phytotherapy Research, 10.1002/ptr.4813, 27 , 7, (1102-1105), (2012). Wiley Online Library
- Young-Jun Lee, Bo-Ra Yoon, Hyeon-Son Choi, Boo-Yong Lee, Ok-Hwan Lee, Effect of Sargassum micracanthum extract on Lipid Accumulation and Reactive Oxygen Species (ROS) Production during Differentiation of 3T3-L1 Preadipocytes, Korean Journal of Food Preservation, 10.11002/kjfp.2012.19.3.455, 19 , 3, (455-461), (2012). Crossref
- Mei Piao, Yu Hyun, Suk Cho, Hee Kang, Eun Yoo, Young Koh, Nam Lee, Mi Ko, Jin Hyun, An Ethanol Extract Derived from Bonnemaisonia hamifera Scavenges Ultraviolet B (UVB) Radiation-Induced Reactive Oxygen Species and Attenuates UVB-Induced Cell Damage in Human Keratinocytes, Marine Drugs, 10.3390/md10122826, 10 , 12, (2826-2845), (2012). Crossref
Volume 47 , Issue 3 June 2011
Pages 548-556 相似文献
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Silybin, the major flavonoid of Silybum marianum, is widely used to treat liver diseases such as hepatocellular carcinoma and cirrhosis-associated insulin resistance. Research so far has focused on its anti-oxidant properties. Here, we demonstrate that silybin and its derivative dehydrosilybin inhibit glucose uptake in several model systems. Both flavonoids dose-dependently reduce basal and insulin-dependent glucose uptake of 3T3-L1 adipocytes, with dehydrosilybin showing significantly stronger inhibition. However, insulin signaling was not impaired, and immunofluorescence and subcellular fractionation showed that insulin-induced translocation of GLUT4 to the plasma membrane is also unchanged. Likewise, hexokinase activity was not affected suggesting that silybin and dehydrosilybin interfere directly with glucose transport across the PM. Expression of GLUT4 in CHO cells counteracted the inhibition of glucose uptake by both flavonoids. Moreover, treatment of CHO cells with silybin and dehydrosilybin reduced cell viability which was partially rescued by GLUT4 expression. Kinetic analysis revealed that silybin and dehydrosilybin inhibit GLUT4-mediated glucose transport in a competitive manner with K(i)=60 and 116 μM, respectively. We conclude that silybin and dehydrosilybin inhibit cellular glucose uptake by directly interacting with GLUT transporters. Glucose starvation offers a novel explanation for the anti-cancer effects of silybin. 相似文献
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Ross SA Song X Burney MW Kasai Y Orlicky DJ 《Biochemical and biophysical research communications》2003,302(2):354-358
3T3-L1 adipocytes have proven difficult to transfect with plasmid-encoded cDNAs or even infect with virally-derived cDNAs. We have developed and characterized a 3T3-L1 adipocyte cell line stably expressing the truncated receptor for coxsackievirus and adenovirus receptor (CAR) for its ability to be infected with adenoviruses at a low multiplicity of infection (m.o.i.). Using green fluorescent protein driven by the cytomegalovirus promoter in adenovirus fiber type 5 we compared infection efficiencies of CAR adipocytes versus the parental 3T3-L1 adipocytes. As assessed by immunofluorescence, CAR adipocytes were infected at approximately 100-fold greater efficiency than regular 3T3-L1 adipocytes. The efficiency of transduction for the CAR adipocytes was >90% at multiplicities of infection of 50 whereas standard adipocytes were poorly transduced even at an m.o.i. of 2000. Since many investigators studying insulin action use 3T3-L1 adipocytes, we compared CAR adipocytes versus regular adipocytes and showed that the two cell lines were similar with respect to insulin stimulation of insulin receptor, MAPK, and Akt phosphorylation and basal- and insulin-stimulated glucose transport. In addition, CAR adipocytes accumulated GLUT4 and SCD1 proteins during the adipogenesis program with the same time course as regular 3T3-L1 adipocytes. Lastly, CAR adipocytes produced and secreted the adipose-specific hormone Acrp30. These data suggest 3T3-L1CARDelta1 adipocytes are virtually indistinguishable from their parental cells, but demonstrate a significant advantage with improved efficiency of adenoviral transduction for gain or deletion of function studies. 相似文献
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目的：观察体外培养条件下3T3-L1脂肪前体细胞诱导分化成的成熟脂肪细胞中解偶联蛋白2（UCP2）mRNA表达水平及黄体酮对其表达的影响。方法：体外培养3T3-L1脂肪细胞,在诱导3T3．L1脂肪细胞分化成熟后,经不同黄体酮浓度10μm／25μM／50μM／75μM/100μM刺激后,抽提总RNA,用RT—PCR检测UCP2mRNA的表达。结果：黄体酮会促进成熟脂肪细胞中UCP2mRNA的表达,（P〈0．05）其中25μM浓度刺激下UCP2mRNA表达量最高。结论：体外培养中,黄体酮对成熟脂肪细胞中UCP2mRNA的表达与调控具有一定的影响。 相似文献