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Overexpression of the trehalose-6-phosphate synthase gene <Emphasis Type="Italic">OsTPS1</Emphasis> enhances abiotic stress tolerance in rice 总被引:1,自引:0,他引:1
Trehalose plays an important role in metabolic regulation and abiotic stress tolerance in a variety of organisms. In plants,
its biosynthesis is catalyzed by two key enzymes: trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase
(TPP). The genome of rice (Oryza sativa) contains 11 OsTPS genes, and only OsTPS1 shows TPS activity. To demonstrate the physiological function of OsTPS1, we introduced it into rice
and found that OsTPS1 overexpression improved the tolerance of rice seedling to cold, high salinity and drought treatments without other significant
phenotypic changes. In transgenic lines overexpressing OsTPS1, trehalose and proline concentrations were higher than in the wild type and some stress-related genes were up-regulated,
including WSI18, RAB16C, HSP70, and ELIP. These results demonstrate that OsTPS1 may enhance the abiotic stress tolerance of plants by increasing the amount of trehalose
and proline, and regulating the expression of stress-related genes. Furthermore, we found that overexpression of some Class
II TPSs also enhanced plant tolerance of abiotic stress. This work will help to clarify the role of trehalose metabolism in abiotic
stress response in higher plants. 相似文献
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We describe here the isolation and characterization of OsiSAP8, a member of stress Associated protein (SAP) gene family from rice characterized by the presence of A20 and AN1 type Zinc
finger domains. OsiSAP8 is a multiple stress inducible gene, induced by various stresses, namely heat, cold, salt, desiccation, submergence, wounding,
heavy metals as well as stress hormone Abscisic acid. OsiSAP8 protein fused to GFP was localized towards the periphery of
the cells in the epidermal cells of infiltrated Nicotiana benthamiana leaves. Yeast two hybrid analysis revealed that A20 and AN1 type zinc-finger domains of OsiSAP8 interact with each other.
Overexpression of the gene in both transgenic tobacco and rice conferred tolerance to salt, drought and cold stress at seed
germination/seedling stage as reflected by percentage of germination and gain in fresh weight after stress recovery. Transgenic
rice plants were tolerant to salt and drought during anthesis stage without any yield penalty as compared to unstressed transgenic
plants.
OsiSAP8 is deposited in the Genbank with the Accession number AY345599. 相似文献
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Water movement across cellular membranes is regulated largely by a family of water channel proteins called aquaporins (AQPs).
Since several abiotic stresses such as, drought, salinity and freezing, manifest themselves via altering water status of plant
cells and are linked by the fact that they all result in cellular dehydration, we overexpressed an AQP (tonoplast intrinsic
protein) from Panax ginseng, PgTIP1, in transgenic Arabidopsis
thaliana plants to test its role in plant’s response to drought, salinity and cold acclimation (induced freezing tolerance). Under
favorable conditions, PgTIP1 overexpression significantly increased plant growth as determined by the biomass production, and leaf and root morphology.
PgTIP1 overexpression had beneficial effect on salt-stress tolerance as indicated by superior growth status and seed germination
of transgenic plants under salt stress; shoots of salt-stressed transgenic plants also accumulated greater amounts of Na+ compared to wild-type plants. Whereas PgTIP1 overexpression diminished the water-deficit tolerance of plants grown in shallow (10 cm deep) pots, the transgenic plants
were significantly more tolerant to water stress when grown in 45 cm deep pots. The rationale for this contrasting response,
apparently, comes from the differences in the root morphology and leaf water channel activity (speed of dehydration/rehydration)
between the transgenic and wild-type plants. Plants overexpressed with PgTIP1 exhibited lower (relative to wild-type control) cold acclimation ability; however, this response was independent of cold-regulated
gene expression. Our results demonstrate a significant function of PgTIP1 in growth and development of plant cells, and suggest that the water movement across tonoplast (via AQP) represents a rate-limiting
factor for plant vigor under favorable growth conditions and also significantly affect responses of plant to drought, salt
and cold stresses. 相似文献
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Jiang SY Bachmann D La H Ma Z Venkatesh PN Ramamoorthy R Ramachandran S 《Plant molecular biology》2007,65(4):385-402
The availability of diversified germplasm resources is the most important for developing improved rice varieties with higher
seed yield or tolerance to various biotic or abiotic stresses. Here we report an efficient tool to create increased variations
in rice by maize Ac/Ds transposon (a gene trap system) insertion mutagenesis. We have generated around 20,000 Ds insertion rice lines of which majority are homozygous for Ds element. We subjected these lines to phenotypic and abiotic stress screens and evaluated these lines with respect to their
seed yields and other agronomic traits as well as their tolerance to drought, salinity and cold. Based on this evaluation,
we observed that random Ds insertions into rice genome have led to diverse variations including a range of morphological and conditional phenotypes.
Such differences in phenotype among these lines were accompanied by differential gene expression revealed by GUS histochemical
staining of gene trapped lines. Among the various phenotypes identified, some Ds lines showed significantly higher grain yield compared to wild-type plants under normal growth conditions indicating that
rice could be improved in grain yield by disrupting certain endogenous genes. In addition, several 1,000s of Ds lines were subjected to abiotic stresses to identify conditional mutants. Subsequent to these screens, over 800 lines responsive
to drought, salinity or cold stress were obtained, suggesting that rice has the genetic potential to survive under abiotic
stresses when appropriate endogenous genes were suppressed. The mutant lines that have higher seed yielding potential or display
higher tolerance to abiotic stresses may be used for rice breeding by conventional backcrossing combining with molecular marker-assisted
selection. In addition, by exploiting the behavior of Ds to leave footprints upon remobilization, we have shown an alternative strategy to develop new rice varieties without foreign
DNA sequences in their genome.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Overexpression of ethylene response factor <Emphasis Type="Italic">TERF2</Emphasis> confers cold tolerance in rice seedlings 总被引:1,自引:0,他引:1
Rice (Oryza sativa L.) is a warm-season plant exposed to various stresses. Low temperature is an important factor limiting extension of rice
cultivation areas and productivity. Previously, we have demonstrated that tomato ERF protein TERF2 enhances freezing tolerance
of transgenic tobacco and tomato plants. Herein, we report that overexpression of TERF2 enhances transgenic rice tolerance to cold without affecting growth or agronomic traits. Physiological assays revealed that
TERF2 could not only increase accumulation of osmotic substances and chlorophyll, but also reduce reactive oxygen species
(ROS) and malondialdehyde (MDA) content and decrease electrolyte leakage in rice under cold stress. Further analysis of gene
expression showed that TERF2 could activate expression of cold-related genes, including OsMyb, OsICE1, OsCDPK7, OsSODB, OsFer1, OsTrx23, and OsLti6, in transgenic rice plants under natural condition or cold stress. Thus, our findings demonstrated that TERF2 modulated expression
of stress-related genes and a series of physiological adjustments under cold stress, indicating that TERF2 might have important
regulatory roles in response to abiotic stress in rice and possess potential utility in improving crop cold tolerance. 相似文献
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Shunwu Yu Aona Huang Jia Li Lin Gao Yanni Feng Erinn Pemberton Chunli Chen 《Plant Growth Regulation》2018,84(3):519-531
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Kumar R Mustafiz A Sahoo KK Sharma V Samanta S Sopory SK Pareek A Singla-Pareek SL 《Plant molecular biology》2012,79(6):555-568
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Coping with different kinds of biotic and abiotic stresses is the foundation of sustainable agriculture. Although conventional breeding and marker-assisted selection are being employed in mulberry (Morus indica L.) to develop better varieties, nonetheless the longer time periods required for these approaches necessitates the use of precise biotechnological approaches for sustainable agriculture. In an attempt to improve stress tolerance of mulberry, an important plant of the sericulture industry, an encoding late embryogenesis abundant gene from barley (HVA1) was introduced into mulberry plants by Agrobacterium-mediated transformation. Transgenic mulberry with barley Hva1 under a constitutive promoter actin1 was shown to enhance drought and salinity tolerance. Here, we report that overexpression of barley Hva1 also confers cold tolerance in transgenic mulberry. Further, barley Hva1 gene under control of a stress-inducible promoter rd29A can effectively negate growth retardation under non-stress conditions and confer stress tolerance in transgenic mulberry. Transgenic lines display normal morphology to enhanced growth and an increased tolerance against drought, salt and cold conditions as measured by free proline, membrane stability index and PSII activity. Protein accumulation was detected under stress conditions confirming inductive expression of HVA1 in transgenics. Investigations to assess stress tolerance of these plants under field conditions revealed an overall better performance than the non-transgenic plants. Enhanced expression of stress responsive genes such as Mi dnaJ and Mi 2-cysperoxidin suggests that Hva1 can regulate downstream genes associated with providing abiotic stress tolerance. The investigation of transgenic lines presented here demonstrates the acquisition of tolerance against drought, salt and cold stress in plants overexpressing barley Hva1, indicating that Arabidopsis rd29A promoter can function in mulberry. 相似文献
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Asadulghani Nitta K Kaneko Y Kojima K Fukuzawa H Kosaka H Nakamoto H 《Archives of microbiology》2004,182(6):487-497
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OsRMC, a negative regulator of salt stress response in rice, is regulated by two AP2/ERF transcription factors 总被引:1,自引:0,他引:1
Tânia S. Serra Duarte D. Figueiredo André M. Cordeiro Diego M. Almeida Tiago Lourenço Isabel A. Abreu Alvaro Sebastián Lisete Fernandes Bruno Contreras-Moreira M. Margarida Oliveira Nelson J. M. Saibo 《Plant molecular biology》2013,82(4-5):439-455