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1.
1 植物名称 山葵 (Eutremawasabi)品种达摩(种子购自云南 )。2 材料类别 秋播苗的顶芽或侧芽。3 培养条件 启动培养基 :( 1 )MS + 6 BA 0 .2mg·L- 1 (单位下同 ) +琼脂 7g·L- 1 +蔗糖 2 0 g·L- 1 。增殖培养基 :( 2 )MS + 6 BA 4+NAA 0 .1 +蔗糖 30g·L- 1 ;( 3)MS + 6 BA 0 .2 +KT 0 .2 +NAA 0 .0 5 +琼脂 7g·L- 1 +蔗糖 30g·L- 1 。 ( 4 )MS + 6 BA 4+NAA 0 .1 +琼脂 7g·L- 1 +蔗糖 30g·L- 1 。生根培养基 :( 5 )MS +NAA 0 .1 +琼脂 7g·L- 1 +蔗糖 30g·…  相似文献   

2.
蒟蒻薯的组织培养及植株再生   总被引:1,自引:0,他引:1  
1 植物名称 薯 (Taccachantrieri) ,又名箭根薯、老虎须、黑蝴蝶等。2 材料类别 种子或茎尖。3 培养条件 种子萌发培养基 :( 1 ) 1 /2MS ;( 2 )MS。芽诱导及增殖培养基 :( 3)MS + 6 BA 3.0~5 .0mg·L- 1 (单位下同 ) +NAA 0 .3~ 0 .0 5 ;( 4 )MS+ 6 BA 2 .0~ 1 .0 +KT 2 .0~ 1 .0 +NAA 0 .0 5。生根培养基 :( 5 )MS + 6 BA 0 .5 +IBA 0 .3;( 6)1 /2MS +IBA 0 .5。以上培养基均附加 0 .7%琼脂 ,pH 5 .4~ 5 .6。培养基 ( 1 )、( 2 )、( 6)的糖用量为 2 0g·L- 1 ;培养…  相似文献   

3.
大叶凤尾蕨的离体培养及植株再生   总被引:8,自引:2,他引:6  
1 植物名称 大叶凤尾蕨 (Pteriscretica)品种白斑大叶凤尾蕨 (cv .“Albolineneata”)。2 材料类别 叶背面成熟孢子。孢子囊群沿羽片顶部以下的叶缘连续分布 ,呈狭条形。供试植株来源于荷兰花卉市场的大叶凤尾蕨盆花。3 培养条件  (1 )诱导孢子萌发培养基 :1 / 3MS ;(2 )初代培养基 :MS 6 BA 3 .0mg·L- 1 (单位下同 ) IBA 3 .0 ;(3 )继代培养基 :MS 6 BA 1 .0 IBA 1 .0 ;(4)生根培养基 :1 / 2MS NAA 2 .0 IAA2 .0 ;(5 )复壮培养基 :1 / 2MS 活性炭 2 0 g·L- 1…  相似文献   

4.
罗布麻茎段的组织培养   总被引:11,自引:0,他引:11  
1 植物名称 罗布麻 (Apocynumvene tum)。2 材料类别 茎段。3 培养条件 培养基为 :( 1 )MS 6 BA 1 .5mg·L-1(单位下同 ) NAA 0 .2 ;( 2 )MS 6 BA 2 NAA 0 .2 ;( 3) 1 /2MS IAA 0 .2。培养基 ( 1 )、( 2 )加蔗糖 30 g·L-  相似文献   

5.
龙蒿的组织培养和快繁   总被引:2,自引:0,他引:2  
1 植物名称 龙蒿 (Artemisiadracunculus) ,又名狭叶青蒿。2 材料类别 嫩尖、茎段。3 培养条件 芽诱导培养基 :(1 ) 1 /3MS 6 BA1 .0mg·L- 1 (单位下同 ) IBA 0 .2。增殖培养基 :(2 )MS 6 BA 0 .5 IBA 0 .2 ;(3 )MS 6 BA 0 .2 IBA 0 .2 ;(4)MS。生根培养基 :(5 ) 1 /2MS IBA 0 .5 ;(6 ) 1 /2MS NAA 0 .5 ;(7) 1 /2MS IBA0 .5 NAA 0 .5。以上培养基加入微生物多糖固化剂 4.5 g·L- 1 ,蔗糖 3 % ,培养温度 (2 5± 2 )℃ ,pH5 .86 .0 ,光照度 1 5 0 0…  相似文献   

6.
樱桃番茄的组织培养与植株再生   总被引:6,自引:0,他引:6  
1 植物名称 樱桃番茄 (Lycopersicumesculentumvar.cerasiforme)。2 材料类别 无菌种子苗下胚轴。3 培养条件 诱导愈伤组织的培养基 :( 1 )MS +6 BA 2mg·L- 1 (单位下同 ) +IAA 0 .2 ;( 2 )MS +6 BA 2 +NAA 0 .2 ;( 3)MS + 6 BA 1 .0 +NAA 0 .1 ;( 4 )MS + 6 BA 1 .0 +IAA 0 .1。诱导分化的培养基 :( 5 )MS + 6 BA 1 .0。生根培养基 :( 6)MS +NAA 0 .1。各种培养基均附加 3%蔗糖 ,0 .6%琼脂 ,pH 5 .8,培养温度为 ( 2 5± 2 )℃ ,每天光照 1 6h ,光…  相似文献   

7.
大白菜真叶的组织培养及植株再生   总被引:15,自引:2,他引:13  
1 植物名称 大白菜 (Brassicacampestrisssp .pekinensis)北京 80号和No .1 0。2 材料类别 无菌苗真叶。3 培养条件  (1 )种子萌发培养基 :MS0 (即MS) ;(2 )芽分化培养基 :MS 6 BA 2mg·L- 1 (单位下同 ) NAA 1 AgNO33 ;(3 )芽伸长及生根培养基 :MS0 。以上培养基均加 3 0 g·L- 1 蔗糖 ,1 0 g·L- 1 琼脂 ,pH 5 .8。AgNO3事先配成 1mg·ml- 1 母液 ,过滤灭菌 ,温度降到 5 0℃左右时加入。光照度为2 0 0 0lx ,光周期 1 6h/8h ,温度 (2 5± 1 )℃。4 生…  相似文献   

8.
植物材料、外植体 培养基 (mg·L- 1 ) 结  果绿梗型和红梗型叶用甘薯 (Ipomoeabatatas) ,由台湾引进。茎尖分生组织(1)初代培养基 :MS +TDZ0 .0 5 +IBA 0 .1,3 %白糖 ;(2 )绿梗型增殖、生根培养基 :MS +TDZ 0 .0 2 +IBA 0 .4,2 %白糖 ;(3 )红梗型增殖、生根培养基 :MS +TDZ 0 .0 1+IBA 0 .2 ,2 %白糖。以上培养基中附加 6g·L- 1 琼脂 ,pH值为 5 .8。光照时间 12h·d- 1 ,光照度 15 0 0~2 0 0 0lx ,培养温度 (2 6± 2 )℃。  健壮种薯在室温 2 5℃以上进行催芽 ,当幼苗长到 3 0cm…  相似文献   

9.
大花蕙兰的组织培养和快速繁殖   总被引:18,自引:0,他引:18  
1 植物名称 大花蕙兰 (Cymbidium grandiflo rum)。2 材料类别 茎段。3 培养条件  (1 )原球茎诱导增殖培养基 :MS +6 BA 0 .5mg·L- 1 (单位下同 ) +NAA 1 .0。 (2 )原球茎分化培养基 :MS +6 BA 2 .0 +NAA 1 .0 +香蕉泥 1 5 0 g·L- 1 。 (3 )生根培养基 ,1 /2MS +NAA1 .0。pH 5 .6~ 5 .8,蔗糖 3 0 g·L- 1 、琼脂 5 g·L- 1固化培养 ,培养温度 (2 5± 2 )℃ ,环境湿度 6 0 %~80 % ,光照度 2 5 0 0lx ,每天光照 1 2h。4 生长与分化情况4.1 启动培养 取大花蕙兰茎段 ,流…  相似文献   

10.
1 植物名称 节瓜 (Benincasahispidavar.chieh qua)品种“四号江心节”。2 材料类别 种子萌发的无菌苗茎尖。3 培养条件  ( 1 )种子萌发培养基 :1 /2MS0 ;( 2 )芽诱导和增殖培养基 :MS + 6 BA 4.0mg·L- 1 (单位下同 ) +IAA 0 .2 ;( 3)伸长培养基 :MS + 6 BA1 .0 +IAA 0 .5 ;( 4 )生根培养基 :MS +IAA 0 .5。培养基 ( 2 )~ ( 4 )均附加 0 .7%琼脂和 3%蔗糖 ,pH5 .8~ 6.0 ,培养温度为 ( 2 5± 2 )℃ ,光照度为 2 0 0 0lx ,光照时间 1 2h·d- 1 。4 生长与分化情况4.1 无…  相似文献   

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Although the importance of the endosperm as a food store inmany angiosperm seeds is well known, its significance duringearly embryogenesis has been neglected. In many interspecifichybrids, and in some other situations, embryos do not developfully and abort. It has often been stated that this is causedby the endosperm failing to conduct sufficient nutrients tothe embryo, but seldom has it been suggested that the endospermactively controls most of the early stages of morphogenesisof the embryo. Information gleaned from a broad survey of theliterature, combined with additional evidence presented here,obtained fromSolanum incanumand interspecific hybrids, indicatethat the endosperm is dynamic and very active in regulatingearly embryo development. This requires highly integrated geneticcontrol of rapidly changing metabolism in the endosperm. Ininterspecific hybrids, lack of coordination may cause unbalancedproduction of growth regulating substances by the endospermand hence abortion of the embryo, or even unregulated productionof nucleases and proteases resulting firstly in autolysis ofthe endosperm and then digestion of the embryo. The endospermmay thus serve to detect inappropriate hybridization of speciesor ploidy levels and so prevent waste of resources by producingseeds that would result in sterile hybrids or unthrifty subsequentgenerations. This discriminatory function of the endosperm hasdiminished during evolution and domestication of the crop plantSolanummelongenaL.Copyright 1998 Annals of Botany Company Solanum, embryo morphogenesis, endosperm, hybrid, seed development.  相似文献   

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14.
Aegidius 《CMAJ》1972,107(3):185
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15.
For most organisms oxygen is essential fo life. When oxygen levels drop below those required to maintain the minimum physiological oxygen requirement of an organism or tissue it is termed hypoxia. To counter act possible deleterious effects of such a state, an immediate molecular response is initiated causing adaptation responses aimed at cell survival. This response is mediated by the hypoxia-inducible factor-1 (HIF-1), which is a heterodimer consisting of an alpha- and a beta-subunit. HIF-1 alpha protein is stabilized under hypoxic conditions and therefore confers selectivity to this response. Hypoxia is characteristic of tumors, mainly because of impaired blood supply resulting from abnormal growth. Over the past few years enormous progress has been made in the attempt to understand how the activation of the physiological response to hypoxia influences neoplastic growth. In this review some aspects of HIF-1 pathway activation in tumors and the consequences for pathophysiology and treatment of neoplasia are discussed.  相似文献   

16.
Pragmatic Women and Body Politics. Margaret Lock and Patricia A. Kaufert. eds. Cambridge, UK: Cambridge University Press, 1998. xii +364 pp.
Maternities and Modernities: Colonial and Postcolonial Experiences in Asia and the Pacific. Kalpana Ram and Margaret Jolly. eds. Cambridge, UK: Cambridge University Press, 1998. xiv. 305 pp.  相似文献   

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Studies of human constitution and mental features are traditionally among the main concepts of anthropological research. In this research, we attempted to discover connections between morphological and constitutional features and self-esteem in children and adolescents. Such a connection was discovered in some studied age groups. The obtained results could serve as a basis for further studies in this field.  相似文献   

20.
《环境昆虫学报》2014,(5):790-804
综述了白蚁螱客的主要种类、共生关系及相关机制的研究进展。白蚁螱客中,已报道的动物种类达170种。在与动物的共生关系中存在偏利共生(宾主共栖和异种共栖)、互利共生和无关共生三种;在与微生物的共生关系中,存在与内生菌(原生动物、细菌、真菌和放线菌)和外生菌(蚁巢伞菌等)间的互利关系。指出了白蚁与螱客研究中存在的问题,给出了解决方案,并提出了今后可能的研究热点或方向,为白蚁的综合利用(如纤维素酶)及今后研究物种间的协同进化提供了基础资料。  相似文献   

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