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1.
《Molecular & cellular proteomics : MCP》2019,18(10):2003-2017
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- •Identification of the first evolutionary divergent sirtuin ScCobB2 in bacteria.
- •Implementing a global quantitative succinylome between ΔScCobB2 and WT cells.
- •ScCobB2 regulates S. coelicolor protein biosynthesis and carbon metabolism pathways.
- •The divergent sirtuin enzymes are prevalent in other groups of Actinobacteria.
2.
《Molecular & cellular proteomics : MCP》2019,18(6):1210-1226
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- •Quantitative global proteome, acetylome and succinylome of phytoplasma-infected Paulownia tomentosa seedlings.
- •Acetylation may be more important than succinylation in response to phytoplasma infection.
- •Acetylation modified the activities of POR and RuBisCO.
- •Possible model to elucidate the molecular mechanism responses to PaWB from proteome and PTMs.
3.
《Molecular & cellular proteomics : MCP》2019,18(7):1410-1427
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- •Quantitative (phoshpo)proteome of primary cell cultures of patient-matched prostate CAF and NPF.
- •Key CAF-associated proteins validated using orthogonal methodologies.
- •LOXL2 inhibitors D-penicillamine and PXS-S2A impaired CAF migration and ECM alignment.
- •Pre-treatment with LOXL2 inhibitors impaired migratory capacity of RWPE-2 cells in co-culture.
4.
《Molecular & cellular proteomics : MCP》2019,18(1):65-85
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- •Natural substrates of FAP were identified using degradomic and proteomic techniques and FAP gene knockout mouse derived embryonic fibroblasts stably transduced with enzymatically active or inactive FAP.
- •Terminal amine isotopic labelling of substrates (TAILS) based degradomics identified cleavage sites in collagens, and many other extracellular matrix (ECM) and associated proteins.
- •Cleavages of lysyl oxidase-like-1, CXCL-5, CSF-1 and C1qT6 by FAP were confirmed in vitro.
- •Differential metabolic labelling coupled with quantitative proteomic analysis implicated FAP in regulating proteins that are associated with ECM, ECM-cell interactions, coagulation, metabolism and wound healing.
5.
《Molecular & cellular proteomics : MCP》2019,18(3):571-575
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- •Incrementally build mzML 1.1, and mzIdentML 1.2 files in Python over a file stream.
- •Traverse controlled vocabularies using common mapping patterns.
- •Generate byte offset index as the document streams.
- •Manage referential integrity on-the-fly.
6.
《Molecular & cellular proteomics : MCP》2019,18(4):642-656
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- •Profiling antibody responses of patients with naturally acquired malaria immunity.
- •High-density peptide arrays featuring linear epitopes.
- •Epitope mapping of known and potential novel vaccine candidates.
- •Novel immunogenic epitopes discovered, and known antibody target motifs confirmed.
7.
Intact Transition Epitope Mapping – Targeted High-Energy Rupture of Extracted Epitopes (ITEM-THREE),
《Molecular & cellular proteomics : MCP》2019,18(8):1543-1555
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- •Multiplex epitope mapping/antigenic determinant identification in the gas phase.
- •Intact transition and controlled dissociation of immune complexes by MS.
- •Simultaneous identification and amino acid sequence determination of epitopes.
- •Simplified in-solution sample handling because of ion manipulation and filtering by MS.
8.
《Molecular & cellular proteomics : MCP》2019,18(11):2138-2148
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- •Highly glycosylated matrisome proteins and their binding partners comprise extracellular networks that mediate tissue-specific cellular microenvironments.
- •Elucidation of roles of matrisome molecules in disease mechanisms requires detailed mapping of matrisome glycosylation and other post-translational modifications.
- •We review tissue workup methods for matrisome proteomics, glycomics and glycoproteomics.
- •The combination of proteomics, glycomics and glycoproteomics profiles matrisome protein modifications distinct from those studied by immunohistochemistry.
9.
《Molecular & cellular proteomics : MCP》2019,18(1):41-50
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- •Proteome of mature boar spermatozoa from cauda epididymal and ejaculated sources were analyzed by iTRAQ-based LC-MS/MS.
- •1,723 sperm proteins identified (974 of Sus scrofa taxonomy).
- •1,602 sperm proteins quantified (960 of Sus scrofa taxonomy).
- •32 Sus scrofa sperm proteins were differentially expressed among sperm sources.
- •The proteome of boar spermatozoa is remodelled during ejaculation.
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11.
《Molecular & cellular proteomics : MCP》2019,18(1):115-126
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- •Application of Sortase A to label protein N-termini across the whole proteome.
- •Novel gel, proteomic and ELISA-based methods to determine N-myristoylation of proteins in cells, without metabolic labelling.
- •Side by side mass spectrometric quantification of changes in protein N-myristoylation by two complementary methods.
- •Improved Biotin-Neutravidin affinity enrichment protocol.
12.
《Molecular & cellular proteomics : MCP》2019,18(8):1572-1587
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- •Protein acetylation at Lys residues and the N terminus occurs widely in Sulfolobus.
- •SisPat preferentially acetylates a group of acyl-CoA synthetases.
- •SisArd1 acetylates the majority of the acetylated N termini identified in the cell.
- •SisArd1, but not SisPat, is required for the optimal growth of the organism.
13.
《Molecular & cellular proteomics : MCP》2019,18(2):169-181
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- •Quantitative proteomics of mitotic chromosome scaffold isolated from chicken DT40 cells.
- •BAZ1B identified in the isolated mitotic chromosome scaffold localizes to mitotic chromosome axes.
- •BAZ1B knockout caused prophase delay because of altered chromosome condensation timing and impaired mitosis progression.
- •BAZ1B knockout did not affect prometaphase chromosome structure.
14.
《Molecular & cellular proteomics : MCP》2019,18(4):773-785
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- •The developed Ac-LysargiNase showed higher stability and activity than before.
- •The merged spectra of the mirror peptides achieved nearly complete ion coverage.
- •pNovoM obviously increased the efficiency and accuracy of peptide sequencing.
- •The mirror enzymatic strategy achieved precision de novo sequencing on proteome scales.
15.
《Molecular & cellular proteomics : MCP》2019,18(8):1630-1650
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- •Our search identifies 2,134 kinase-substrate phosphosite pairs in breast cancer.
- •CDKs and MAPKs are dominant regulators of trans substrate-phosphorylation.
- •Druggability, outcomes, and immune signatures related to kinase-substrates.
- •Experimentally validated activated phosphosites of ERBB2, EIF4EBP1, and EGFR.
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17.
《Molecular & cellular proteomics : MCP》2019,18(2):182-199
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- •An integrated proteome, phosphoproteome and glycoproteome analysis was applied to past P. falciparum-infected placentas.
- •A total of 2946 proteins, 1733 N-linked glycosites and 4100 phosphosites were identified and quantified in the human placentas.
- •AKT and ERK signaling pathways are associated to an increased apoptosis in past P. falciparum-infected placentas.
18.
《Molecular & cellular proteomics : MCP》2018,17(12):2358-2370
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- •First comparative proteomic analysis of white and brown adipocyte secretomes.
- •100 novel adipokines differentially secreted from white versus brown adipocytes.
- •Functionally enriched protein class changes in white and brown adipocytes.
- •200 novel, NE-responsive adipokines from brown adipocytes.
19.
《Molecular & cellular proteomics : MCP》2019,18(11):2191-2206
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- •Quantitative high-throughput glycoanalytical technology as a diagnostic tool for ovarian cancer detection.
- •Multiplexed approach harnessing N-glycan data for six glycoproteins from a single biological sample.
- •Detailed characterization of human serum N-glycans from antibodies IgG, IgM and IgA and acute phase proteins transferrin, haptoglobin and alpha-1-antitrypsin.
- •Structural differences in antibody and acute phase protein glycosylation for mechanistic insights.