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水稻土施硅对土壤-水稻系统中镉的降低效果 总被引:7,自引:0,他引:7
水稻中镉的积累造成人类健康的风险,增加水稻硅素能减轻镉中毒症状,降低稻米镉积累,但是硅对重金属的作用机理尚不清楚。主要研究了在中度和高度镉污染的土壤中,通过施用固态和液态的富硅物质对土壤-水稻系统中镉的吸收和转运的影响,探明决定镉和硅在根与芽的质外体和共质体中的作用机理。试验结果表明:(1)在中度和高度污染的土壤中,镉在土壤-作物系统中的转移和积累情况是不同的,可以通过富硅物质中的单硅酸与镉离子的相互作用,增加镉在硅物质表面的吸附来减少镉在土壤中的流动;(2)富硅物质可以降低水稻根和芽中镉的积累,在高度镉污染的情况下,施用硅可以使镉大量积累在水稻根及其共质体中,并降低根及其共质体中镉的转换和积累;(3)新鲜土壤中水萃取态的单硅酸含量与镉在土壤-作物系统中的流动性、转运以及积累等主要参数密切相关。 相似文献
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施硅对抑制植物吸收重金属镉的效应研究进展 总被引:6,自引:0,他引:6
施用化学改良剂是控制土壤重金属污染的有效手段。研究施硅对抑制植物吸收重金属镉的影响及其作用机制,对促进利用硅肥作为改良剂治理重金属污染土壤技术的发展有重要意义。近年来,以硅肥作改良剂对重金属污染的土壤进行治理的研究大量涌现。本文从施硅对抑制植物吸收镉及镉在植物体内的分布、迁移的影响;从植物细胞膜透性、抗氧化物酶系和抗氧化剂等新陈代谢或生理过程及硅-金属复合物的结构组成等方面对植物抗镉胁迫的生理生化效应及其抑制植物吸收重金属镉的机制进行综述,并对今后有待进一步研究的问题提出了建议。 相似文献
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绿藻的镉结合蛋白及其耐镉性初探 总被引:5,自引:0,他引:5
蛋白核小球藻及斜生栅藻经镉处理后,从细胞可溶性成分中,分离出镉结合蛋白,分子量各为12 600及12 000。未经镉处理的藻体中不存在这类蛋白。用聚丙烯酰胺凝胶电泳证明此为纯蛋白,在250 nm处有特征吸收峰,但氨基酸成分分析证明,并非是典型的镉硫蛋白。镉结合蛋白能够结合进入细胞内的40~50%的镉,随镉浓度加大,镉结合量增加,至一定高浓度时,镉结合蛋白增加不明显。镉结合蛋白的形成,使镉以无毒形式存在,限制镉与苹果酸脱氢酶等物质的结合,限制镉以自由态存在。可以认为镉结合蛋白是两种绿藻耐镉的生物化学机制之一,而蛋白与镉结合量的饱和则是藻体出现镉害症状的重要原因。 相似文献
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以不同品种桃(Amygdlus persica(L.)Batsch)"大久保(Okubao)"和"丽春(Lichun)"为试材,研究了不同土壤镉水平下桃树对镉及矿质养分的吸收.结果表明:随着镉浓度增大,品种间镉、大量元素、微量元素的含量差异显著(P<0.05);桃树器官中镉积累量为根>茎>叶>果实;土壤中镉浓度为10 mg·kg-1时,大久保外果皮中镉积累量最大,较对照增加了226.78%,而丽春桃果实组织中镉积累量在处理前后没有明显变化;当土壤镉浓度超过20 mg·kg-1时,2品种均不能正常座果.土壤镉浓度为100 mg·kg-1,胁迫时间为9个月后,大久保桃树体死亡;土壤Cd浓度<20 mg·kg-1时促进大量元素K、N、Ca、Mg 和微量元素Zn、Cu、Mn、Fe吸收,>20 mg·kg-1时抑制其吸收,但丽春根和大久保茎中Fe 元素含量呈现持续上升趋势,且Cd 100 mg·kg-1时Fe的吸收值达最大. 相似文献
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重金属镉对水稻根毛细胞钾离子吸收过程的影响 总被引:1,自引:0,他引:1
重金属镉进入植物体后会引起一系列的毒害反应,然而迄今为止有关镉毒害的机制并不明了。本研究综合使用了膜电势测定、非损伤微测和膜片钳等电生理学实验技术,检测了镉对水稻根毛细胞钾离子吸收过程的影响。研究结果显示,外源施用50μmol·L-1 CdCl2能明显造成水稻根毛细胞膜的去极化,抑制根毛细胞质膜内向K+通道活性,同时诱导外向K+通道开放,导致根系内K+外渗,进而降低了水稻根部K+含量。通过上述影响,镉扰乱了水稻根系对钾离子的吸收过程,造成钾元素缺失,成为植物体镉毒害作用的机理之一。 相似文献
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镉暴露对黑斑蛙精巢ROS的诱导及其蛋白质氧化损伤作用机理 总被引:3,自引:0,他引:3
重金属镉对精巢发育、呼吸及神经系统信号转导等途径均有不良影响,被认为是造成两栖动物种群数量急剧下降的重要原因之一。然而,有关镉对精巢损伤的分子机理还不清楚。通过对镉暴露后的黑斑蛙精巢活性氧自由基(ROS)、蛋白质羰基(PCO)以及DNA蛋白质交联(DPC)等指标的系统分析,探讨了镉对精巢毒害的分子作用机理。随镉浓度的增加,黑斑蛙精巢细胞线粒体ROS随镉暴露浓度的增加而升高,0.5、1.0 mg/L镉染毒组与对照组比较有显著性差异(P<0.05);精巢组织PCO和DPC也随镉暴露浓度的增加而逐渐上升,且均呈明显的浓度-效应关系。结果表明:镉诱导机体产生ROS,进而导致蛋白质氧化损伤以及DNA损伤,说明精巢组织ROS的产生是镉致雄性生殖毒效应机制的重要因素之一。 相似文献
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Fujishiro H Yano Y Takada Y Tanihara M Himeno S 《Metallomics : integrated biometal science》2012,4(7):700-708
Chronic exposure to cadmium causes preferential accumulation of cadmium in the kidney, leading to nephrotoxicity. In the process of renal cadmium accumulation, the cadmium bound to a low-molecular-weight metal-binding protein, metallothionein, has been considered to play an important role in reabsorption by epithelial cells of proximal tubules in the kidney. However, the role and mechanism of the transport of Cd(2+) ions in proximal tubule cells remain unclear. Zinc transporters such as Zrt, Irt-related protein 8 (ZIP8) and ZIP14, and divalent metal transporter 1 (DMT1) have been reported to have affinities for Cd(2+) and Mn(2+). To examine the roles of these metal transporters in the absorption of luminal Cd(2+) and Mn(2+) into proximal tubule cells, we utilized a cell culture system, in which apical and basolateral transport of metals can be separately examined. The uptake of Cd(2+) and Mn(2+) from the apical side of proximal tubule cells was inhibited by simultaneous addition of Mn(2+) and Cd(2+), respectively. The knockdown of ZIP8, ZIP14 or DMT1 by siRNA transfection significantly reduced the uptake of Cd(2+) and Mn(2+) from the apical membrane. The excretion of Cd(2+) and Mn(2+) was detected predominantly in the apical side of the proximal tubule cells. In situ hybridization of these transporters revealed that ZIP8 and ZIP14 are highly expressed in the proximal tubules of the outer stripe of the outer medulla. These results suggest that ZIP8 and ZIP14 expressed in the S3 segment of proximal tubules play significant roles in the absorption of Cd(2+) and Mn(2+) in the kidney. 相似文献
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木本植物对大气重金属污染物——铅、镉、铜、锌吸收积累作用的研究 总被引:2,自引:0,他引:2
对环境污染除必须采取人工治理措施外,还必须充分发挥自然净化作用,才能取得经济合理和维护生态系统动态平衡的目的。 绿色高等植物特别是树木,对环境污染起着明显的净化和改善作用。因为它们的生物产量高,占有很大的空间,有巨大的根、茎、叶面积作用于环境,并对污染物具有一定的吸收积累或降解作用;生活周期长,有较长的时间与周围环境相互作用与影响,同时吸收积累的污染物质不会在短期内转移释放到环境中;此外,大部分树木是以保护环境或生产木材为主要目的,因而吸收积累的污染物不进入食物链对人产生危害。所以树木在保持和维护生态系 相似文献
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Giuseppe Cannino Elisa Ferruggia Claudio Luparello Anna Maria Rinaldi 《Mitochondrion》2009,9(6):377-384
The heavy metal cadmium (Cd) a pollutant associated with several modern industrial processes, is absorbed in significant quantities from cigarette smoke, water, food and air contaminations.It is known to have numerous undesirable effects on health in both experimental animals and humans, targeting kidney, liver and vascular system.The molecular mechanism accounting for most of the biological effects of Cd are not well-understood and the toxicity targets are largely unidentified.The present review focuses on important recent advances about the effects of cadmium on mitochondria of mammalian cells.Mitochondria are the proverbial powerhouses of the cell, running the fundamental biochemical processes that produce energy from nutrients using oxygen. They are among the key intracellular targets for different stressors including Cd.This review provides new additional informations on the cellular and molecular aspects of the interaction between Cd and cells, emphasizing alterations of mitochondria as important events in Cd cytotoxicity, thus representing an important basis for understanding the mechanisms of cadmium effect on the cells. 相似文献
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Cédric Astier Vincent Gloaguen Céline Faugeron 《International journal of phytoremediation》2014,16(7-8):790-803
Douglas fir trees grown on an artificially Cd-contaminated soil, can tolerate this trace element (up to 68 mg/kg in soil) during several months. Most of the absorbed Cd is retained in roots (25 mg/kg DM), but transfer to aerial part is also effective. Showing the highest content, up to 6 mg/kg DM, among all the aboveground parts, barks seem to be a preferred storage compartment. However, the transfer factor is quite low, about 0.3. Another objective of this study was to compare the cell wall components of trees exposed to increasing Cd amounts in soil. A decrease in lignin and an increase in pectin contents were observed in response to increasing soil cadmium concentration. A concurrent reduction in methyl-esterification of pectin suggests than the structure of this major binding site could therefore be modified as a reaction to cadmium contamination. Future prospects will focus on the modulation of pectin composition in response to Cd exposure. 相似文献
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The purpose of the present study was to evaluate the effect of cadmium on some protein digestive and absorption enzymes in
rats. Thirty-six rats were grouped into three groups of 12 animals each; one group received deionised water and acted as control.
One group received 445 μM Cd and the last group received 890 μM Cd in their drinking water for a period of one month. The
results obtained indicate that increasing the level of cadmium from 445 μM to 890 μM in the drinking water of the rats led
to 29 and 23 increase in accumulated cadmium in the proximal and distal small intestine respectively. The body weight gain
of rats exposed to 445 μM and 890 μMCd was decreased by about 24 and 43 respectively when compared with the control. The activities
of carboxypeptidase A, dipeptidase and Na+/K+ ATPase were reduced in the mucosa of the proximal end of the small intestine
of cadmium exposed rats. The reduction was dose dependent; with the 890 μM Cd exposed rats displaying the least activities.
In the distal small intestine, the activities of these enzymes were restored in the 445 μM Cd exposed rats to levels that
were not statistically different (P>0.05) from those observed in the controls. In the 890 μMCd exposed rats, dipeptidase activity
improved by about 80 compared with the activity of the enzyme in the proximal small intestine. Likewise, Na+/K+ ATPase activity
increased by about 125 compared with the observed level in the proximal small intestine. The study suggests that cadmium given
to rats in drinking water compromise protein digestion and absorption of nutrients particularly in the proximal region of
small intestine and could account for weight reduction associated with cadmium toxicity.
Published online December 2004 相似文献
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Mousa SA 《Life sciences》2004,75(1):93-105
Inflammatory processes play a major role in the secondary injury of the liver produced by cadmium (Cd), and infiltration of neutrophils at the site of necrosis is a common observation. Although the infiltration of leukocytes (mainly neutrophils) into sites of injuried tissue within liver during Cd toxicity is mediated by adhesion molecules, little is known about expression of these adhesion molecules during Cd hepatotoxicity. In the present study, the expression of E-, P-selectin, intracellular adhesion molecule-1 (ICAM-1) and platelet-endothelial adhesion molecule-1 (PECAM-1) was analyzed by immunohistochemistry and immunofluoresence during Cd-induced hepatotoxicity in male rats. In contrast to E-, and P-selectin, ICAM-1 and PECAM-1 were constitutively expressed on sinusoidal endothelial cells of control liver. However, P-selectin was not induced within the liver by Cd administration, whereas E-selectin expression was induced in the liver with a marked increase in immunostaining on sinusoidal endothelial cells from 12 h to 7 days. Also, there was an upregulation in ICAM-1 immunostaining on sinusoidal endothelial cells from 12 h to 7 days after Cd administration, whereas there was no obvious change of PECAM-1 immunostaining on sinusoidal endothelial cells until 24 h. However, PECAM-1 expression was markedly decreased at 48 h but significantly increased at 7 days after Cd administration compared to control liver. Taken together, upregulation of E-selectin and ICAM-1 with biphasic changes in PECAM-1 expression within liver after Cd administration suggests an important role for these adhesion molecules during Cd hepatoxicity. 相似文献
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超积累植物伴矿景天镉耐受基因SpMT2的分离及功能鉴定 总被引:1,自引:0,他引:1
超积累植物由于其对重金属具有地上部超积累以及超耐受等特性,不仅是研究植物离子转运及毒性耐受的理想模式,而且在植物修复的发展和应用中具有不可替代的作用。伴矿景天是近年在我国境内发现的一种景天科镉(Cd)/锌(Zn)超积累植物。为鉴定其富集和耐受Cd的关键基因,笔者构建了其酵母表达cDNA文库,利用酵母的遗传互补系统筛选到一个极大提高了酵母抗Cd能力的基因SpMT2。SpMT2属于富含半胱氨酸(Cys)的金属硫蛋白(Metallothionein)家族。亚细胞定位表明SpMT2表达于酵母细胞质中,并特异地提高酵母对Cd的抗性。进一步研究发现SpMT2的表达显著降低了酵母液泡中Cd含量,但酵母吸收的总Cd含量无显著变化。推测抗性增加是由于SpMT2在酵母细胞质中通过螯合Cd从而降低Cd对酵母的毒害。qRT-PCR分析表明SpMT2在伴矿景天的根和地上部都高丰度表达,且不受Cd诱导变化。鉴于SpMT2也定位于植物细胞质中,结合上述结果,推测SpMT2可能在伴矿景天细胞质中螯合Cd,在降低Cd毒害的同时可能还保持Cd在细胞质中的流动性,从而在Cd长途转运过程中也发挥重要作用。 相似文献
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Achard-Joris M van den Berg van Saparoea HB Driessen AJ Bourdineaud JP 《Biochemistry》2005,44(15):5916-5922
The human MDR1 gene is induced by cadmium exposure although no resistance to this metal is observed in human cells overexpressing hMDR1. To access the role of MDR proteins in cadmium resistance, human MDR1, Lactococcus lactis lmrA, and Oenococcus oeni omrA were expressed in an Escherichia coli tolC mutant strain which proved to be hypersensitive to cadmium. Both the human and bacterial MDR genes conferred cadmium resistance to E. coli up to 0.4 mM concentration. Protection was abolished by 100 microM verapamil. Quantification of intracellular cadmium concentration by atomic absorption spectrometry showed a reduced cadmium accumulation in cells expressing the MDR genes. Inside-out membrane vesicles of L. lactis overexpressing lmrA displayed an ATP-dependent (109)Cd(2+) uptake that was stimulated by glutathione. An evolutionary model is discussed in which MDR proteins have evolved independently from an ancestor protein displaying both organic xenobiotic- and divalent metal-extrusion abilities. 相似文献