共查询到20条相似文献,搜索用时 15 毫秒
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Joanne L Fothergill Stavroula Panagea Charles A Hart Martin J Walshaw Tyrone L Pitt Craig Winstanley 《BMC microbiology》2007,7(1):45
Background
Some isolates of the Liverpool cystic fibrosis epidemic strain of Pseudomonas aeruginosa exhibit an unusual virulence-related phenotype, characterized by over-production of quorum sensing-regulated exoproducts such as pyocyanin and LasA protease. Our aim was to determine the prevalence of this unusual phenotype amongst isolates of the epidemic strain, and to study other intraclonal phenotypic and genotypic variations. 相似文献3.
Nelson C Soares Maria P Cabral José R Parreira Carmen Gayoso Maria J Barba Germán Bou 《Proteome science》2009,7(1):37-10
Background
Acinetobacter baumannii is a nosocomial pathogen that has been associated with outbreak infections in hospitals. Despite increasing awareness about this bacterium, its proteome remains poorly characterised, however recently the complete genome of A. baumannii reference strain ATCC 17978 has been sequenced. Here, we have used 2-DE and MALDI-TOF/TOF approach to characterise the proteome of this strain. 相似文献4.
Adam Akram Marc Ongena Francéline Duby Jacques Dommes Philippe Thonart 《BMC plant biology》2008,8(1):113
Background
Previous studies showed the ability of Pseudomonas putida strain BTP1 to promote induced systemic resistance (ISR) in different host plants. Since ISR is long-lasting and not conducive for development of resistance of the targeted pathogen, this phenomenon can take part of disease control strategies. However, in spite of the numerous examples of ISR induced by PGPR in plants, only a few biochemical studies have associated the protective effect with specific host metabolic changes. 相似文献5.
Laurent X Nouvel Tiago Dos Vultos Eric Kassa-Kelembho Jean Rauzier Brigitte Gicquel 《BMC microbiology》2007,7(1):39
Background
Previous studies have suggested that variations in DNA repair genes of W-Beijing strains may have led to transient mutator phenotypes which in turn may have contributed to host adaptation of this strain family. Single nucleotide polymorphism (SNP) in the DNA repair gene mutT1 was identified in MDR-prone strains from the Central African Republic. A Mycobacteriumtuberculosis H37Rv mutant inactivated in two DNA repair genes, namely ada/alkA and ogt, was shown to display a hypermutator phenotype. We then looked for polymorphisms in these genes in Central African Republic strains (CAR). 相似文献6.
Background
Saccharomyces cerevisiae is the first eukaryotic organism for which a multi-compartment genome-scale metabolic model was constructed. Since then a sequence of improved metabolic reconstructions for yeast has been introduced. These metabolic models have been extensively used to elucidate the organizational principles of yeast metabolism and drive yeast strain engineering strategies for targeted overproductions. They have also served as a starting point and a benchmark for the reconstruction of genome-scale metabolic models for other eukaryotic organisms. In spite of the successive improvements in the details of the described metabolic processes, even the recent yeast model (i.e., i MM904) remains significantly less predictive than the latest E. coli model (i.e., i AF1260). This is manifested by its significantly lower specificity in predicting the outcome of grow/no grow experiments in comparison to the E. coli model. 相似文献7.
Irene Kouskoumvekaki Zhiyong Yang Svava Ó Jónsdóttir Lisbeth Olsson Gianni Panagiotou 《BMC bioinformatics》2008,9(1):59
Background
In the present investigation, we have used an exhaustive metabolite profiling approach to search for biomarkers in recombinantAspergillus nidulans(mutants that produce the 6- methyl salicylic acid polyketide molecule) for application in metabolic engineering. 相似文献8.
Clifford DL Folmes Grzegorz Sawicki Virgilio JJ Cadete Grant Masson Amy J Barr Gary D Lopaschuk 《Proteome science》2010,8(1):38
Background
During and following myocardial ischemia, glucose oxidation rates are low and fatty acids dominate as a source of oxidative metabolism. This metabolic phenotype is associated with contractile dysfunction during reperfusion. To determine the mechanism of this reliance on fatty acid oxidation as a source of ATP generation, a functional proteomics approach was utilized. 相似文献9.
Hsiang Ho Tijana Milenković Vesna Memišević Jayavani Aruri Nataša Pržulj Anand K Ganesan 《BMC systems biology》2010,4(1):84
Background
RNA-mediated interference (RNAi)-based functional genomics is a systems-level approach to identify novel genes that control biological phenotypes. Existing computational approaches can identify individual genes from RNAi datasets that regulate a given biological process. However, currently available methods cannot identify which RNAi screen "hits" are novel components of well-characterized biological pathways known to regulate the interrogated phenotype. In this study, we describe a method to identify genes from RNAi datasets that are novel components of known biological pathways. We experimentally validate our approach in the context of a recently completed RNAi screen to identify novel regulators of melanogenesis. 相似文献10.
Xiao-Jing Zhu Jie-Qiong Dai Xin Tan Yang Zhao Wei-Jun Yang 《BMC developmental biology》2009,9(1):21-8
Background
Cysts of Artemia can remain in a dormant state for long periods with a very low metabolic rate, and only resume their development with the approach of favorable conditions. The post-diapause development is a very complicated process involving a variety of metabolic and biochemical events. However, the intrinsic mechanisms that regulate this process are unclear. 相似文献11.
Jun Hyoung Lee Bong Hyun Sung Mi Sun Kim Frederick R Blattner Byoung Hoon Yoon Jung Hoe Kim Sun Chang Kim 《Microbial cell factories》2009,8(1):2-12
Background
Deletion of large blocks of nonessential genes that are not needed for metabolic pathways of interest can reduce the production of unwanted by-products, increase genome stability, and streamline metabolism without physiological compromise. Researchers have recently constructed a reduced-genome Escherichia coli strain MDS42 that lacks 14.3% of its chromosome. 相似文献12.
Adelfo Escalante Rocío Calderón Araceli Valdivia Ramón de Anda Georgina Hernández Octavio T Ramírez Guillermo Gosset Francisco Bolívar 《Microbial cell factories》2010,9(1):21
Background
Shikimic acid (SA) is utilized in the synthesis of oseltamivir-phosphate, an anti-influenza drug. In this work, metabolic engineering approaches were employed to produce SA in Escherichia coli strains derived from an evolved strain (PB12) lacking the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS-) but with capacity to grow on glucose. Derivatives of PB12 strain were constructed to determine the effects of inactivating aroK, aroL, pykF or pykA and the expression of plasmid-coded genes aroG fbr, tktA, aroB and aroE, on SA synthesis. 相似文献13.
Analysis of optimal phenotypic space using elementary modes as applied to Corynebacterium glutamicum
Background
Quantification of the metabolic network of an organism offers insights into possible ways of developing mutant strain for better productivity of an extracellular metabolite. The first step in this quantification is the enumeration of stoichiometries of all reactions occurring in a metabolic network. The structural details of the network in combination with experimentally observed accumulation rates of external metabolites can yield flux distribution at steady state. One such methodology for quantification is the use of elementary modes, which are minimal set of enzymes connecting external metabolites. Here, we have used a linear objective function subject to elementary modes as constraint to determine the fluxes in the metabolic network of Corynebacterium glutamicum. The feasible phenotypic space was evaluated at various combinations of oxygen and ammonia uptake rates. 相似文献14.
Miyako Kusano Atsushi Fukushima Masanori Arita Pär Jonsson Thomas Moritz Makoto Kobayashi Naomi Hayashi Takayuki Tohge Kazuki Saito 《BMC systems biology》2007,1(1):53-17
Background
Metabolites are not only the catalytic products of enzymatic reactions but also the active regulators or the ultimate phenotype of metabolic homeostasis in highly complex cellular processes. The modes of regulation at the metabolome level can be revealed by metabolic networks. We investigated the metabolic network between wild-type and 2 mutant (methionine-over accumulation 1 [mto1] and transparent testa4 [tt4]) plants regarding the alteration of metabolite accumulation in Arabidopsis thaliana. 相似文献15.
Erik L Hendrickson Qiangwei Xia Tiansong Wang Richard J Lamont Murray Hackett 《BMC microbiology》2009,9(1):185
Background
Porphyromonas gingivalis is a Gram-negative intracellular pathogen associated with periodontal disease. We have previously reported on whole-cell quantitative proteomic analyses to investigate the differential expression of virulence factors as the organism transitions from an extracellular to intracellular lifestyle. The original results with the invasive strain P. gingivalis ATCC 33277 were obtained using the genome sequence available at the time, strain W83 [GenBank: AE015924]. We present here a re-processed dataset using the recently published genome annotation specific for strain ATCC 33277 [GenBank: AP009380] and an analysis of differential abundance based on metabolic pathways rather than individual proteins. 相似文献16.
Rafael Balaña-Fouce Carlos García-Estrada Yolanda Pérez-Pertejo Rosa M Reguera 《BMC microbiology》2008,8(1):113
Background
The unusual heterodimeric leishmanial DNA topoisomerase IB consists of a large subunit containing the phylogenetically conserved "core" domain, and a small subunit harboring the C-terminal region with the characteristic tyrosine residue in the active site. RNAi silencing of any of both protomers induces a non-viable phenotype in the hemoflagelateTrypanosoma brucei. Unfortunately, this approach is not suitable inLeishmaniawhere gene replacement with an antibiotic marker is the only approach to generate lack-of-function mutants. In this work, we have successfully generated null mutants in the small subunit of theL. majorDNA topoisomerase IB using two selection markers, each conferring resistance to hygromycin B and puromycin, respectively. 相似文献17.
Liang Ma Stephanie Taylor Jørgen S Jensen Leann Myers Rebecca Lillis David H Martin 《BMC microbiology》2008,8(1):130
Background
Several methods have been reported for strain typing of Mycoplasma genitalium. The value of these methods has never been comparatively assessed. The aims of this study were: 1) to identify new potential genetic markers based on an analysis of short tandem repeat (STR) sequences in the published M. genitalium genome sequence; 2) to apply previously and newly identified markers to a panel of clinical strains in order to determine the optimal combination for an efficient multi-locus genotyping system; 3) to further confirm sexual transmission of M. genitalium using the newly developed system. 相似文献18.
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Jingshan?Ren Sarah?Sainsbury Nick?S?Berrow David?Alderton Joanne?E?Nettleship David?K?Stammers Nigel?J?Saunders Raymond?J?Owens
Background
The NMB0736 gene of Neisseria meningitidis serogroup B strain MC58 encodes the putative nitrogen regulatory protein, IIANtr (abbreviated to NM-IIANtr). The homologous protein present in Escherichia coli is implicated in the control of nitrogen assimilation. As part of a structural proteomics approach to the study of pathogenic Neisseria spp., we have selected this protein for structure determination by X-ray crystallography. 相似文献20.
Bo Chang Richard S Smith Maureen Peters Olga V Savinova Norman L Hawes Adriana Zabaleta Steven Nusinowitz Janice E Martin Muriel L Davisson Constance L Cepko Brigid LM Hogan Simon WM John 《BMC genetics》2001,2(1):1-12