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1.
The pollen genome of Echinacea purpurea ‘Magnus’ (purple coneflower) was amplified using a modified primer extension pre-amplification (PEP) procedures followed
by AFLP (amplified fragment length polymorphism) analyses of individual pollen grains. Twenty-four AFLP primer pairs were
identified that produced 104 scorable markers in eleven linkage groups. This is the first report on the production of AFLP
markers from individual pollen grains for genetic mapping in coneflower. Coneflowers are important to both the ornamental
and medicinal herb industries. 相似文献
2.
Changes in AFLP and SSR DNA polymorphisms induced by short-term space flight of rice seeds 总被引:1,自引:0,他引:1
J. Y. Lu W. L. Zhang H. Xue Y. Pan C. H. Zhang X. H. He M. Liu 《Biologia Plantarum》2010,54(1):112-116
Differences of both amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) polymorphisms were compared
between the 60-d-old rice (Oryza sativa L. cv. DH7) and F3 rice plants (SP3) derived from seed, which endured a 7-d-space flight in March 2002. Total leaf AFLP DNA
bands amplified from 22 primer pairs were 537 in DH7, whereas 562 in SP3. From the total 267 SSR DNA bands generated by 267
primer pairs, 39 were polymorphic with 22 larger (56 %) or 17 smaller (44 %) fragment size bands. The greatest numbers of
AFLP DNA bands were amplified by primer E1M1 in DH7 (33) and E3M1 in SP3 (35), whilst the least by E4M3 in DH7 (14) and E5M2
in SP3 (16). 相似文献
3.
Conversion of AFLP markers to sequence-specific markers for closely related lines in rice by use of the rice genome sequence 总被引:1,自引:0,他引:1
Shirasawa Kenta Kishitani Sachie Nishio Takeshi 《Molecular breeding : new strategies in plant improvement》2004,14(3):283-292
DNA polymorphism between two major japonica rice cultivars, Nipponbare and Koshihikari, was identified by AFLP. Eighty-four polymorphic AFLP markers were obtained by analysis with 360 combinations of primer pairs. Nucleotide sequences of 73 markers, 29 from Nipponbare and 44 from Koshihikari, were determined, and 46 AFLP markers could be assigned to rice chromosomes based on sequence homology to the rice genome sequence. Specific primers were designed for amplification of the regions covering the AFLP markers and the flanking sequences. Out of the 46 primer pairs, 44 amplified single DNA fragments, six of which showed different sizes between Nipponbare and Koshihikari, yielding codominant SCAR markers. Eight primer pairs amplified only Nipponbare sequences, providing dominant SCAR markers. DNA fragments amplified by 13 primer pairs showed polymorphism by CAPS, and polymorphism of those amplified by 13 other primer pairs were detected by PCR-RF-SSCP (PRS). Nucleotide sequences of the other four DNA fragments were determined in Koshihikari, but no difference was found between Koshihikari and Nipponbare. In total, 40 sequence-specific markers for the combination of Nipponbare and Koshihikari were produced. All the SNPs identified by AFLP were detectable by CAPS and PRS. The same method was applicable to a combination of Kokoromachi and Tohoku 168, and 23 polymorphic markers were identified using these two rice cultivars. The procedure of conversion of AFLP-markers to the sequence-specific markers used in this study enables efficient sequence-specific marker production for closely related cultivars. 相似文献
4.
Madhu Koundal D. R. Sharma T. Mohapatra K. R. Koundal 《Journal of plant biochemistry and biotechnology.》2006,15(1):15-19
The present investigation was carried out with an objective of evaluating genetic diversity in brinjal (Solanum melongena) using DNA markers. A total of 38 brinjal accessions including one wild-species, Solanum sisymbrifolium were characterized using random amplified polymorphic DNA (RAP D) and amplified fragment length polymorphism (AFLP) techniques. Out of 45 primers employed to generate RAPD profiles, reproducible patterns were obtained with 32 primers and 30 (93.7%) of these detected polymorphism. A total of 149 bands were obtained, out of which 108 (72.4%) were polymorphic. AFLP analysis was carried out using four primer combinations. Each of these primers was highly polymorphic. Out of 253 fragments amplified from these four primer combinations, 237 (93.6%) were polymorphic. The extent of pair-wise similarity ranged from 0.264 to 0.946 with a mean of 0.787 in RAPD, in contrast to a range of 0.103 to 0.847 with a mean of 0.434 in AFLP. The wild species clustered separately from the brinjal genotypes. In the dendrogram constructed separately using RAPD and AFLP markers, the brinjal genotypes were grouped into clusters and sub-clusters, and the varieties released by IARI remained together on both the dendrograms. All the 30 RAPD primers in combination and each of the four primer pairs in AFLP could distinguish the brinjal accessions from each other. AFLP was thus found to be more efficient than RAPD in estimation of genetic diversity and differentiation of varieties in brinjal. 相似文献
5.
Cleaved AFLP (cAFLP), a modified amplified fragment length polymorphism analysis for cotton 总被引:3,自引:0,他引:3
Zhang J Lu Y Yu S 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2005,111(7):1385-1395
In certain plant species including cotton (Gossypium hirsutum L. or Gossypium barbadense L.), the level of amplified fragment length polymorphism (AFLP) is relatively low, limiting its utilization in the development
of genome-wide linkage maps. We propose the use of frequent restriction enzymes in combination with AFLP to cleave the AFLP
fragments, called cleaved AFLP analysis (cAFLP). Using four Upland cotton genotypes (G. hirsutum) and three Pima cotton (G. barbadense), we demonstrated that cAFLP generated 67% and 132% more polymorphic markers than AFLP in Upland and Pima cotton, respectively.
This resulted in 15.5 and 25.5 polymorphic cAFLP markers per AFLP primer combination, as compared to 9.1 and 11.0 polymorphic
AFLP. The cAFLP-based genetic similarity (GS) is generally lower than the AFLP-based GS, even though both marker systems are
overall congruent. In some cases, cAFLP can better resolve genetic relationships between genotypes, rendering a higher discriminatory
power. Given the high-resolution power of capillary-based DNA sequencing system, we further propose that AFLP and cAFLP amplicons
from the same primer combination can be pooled as one sample before electrophoresis. The combination produced an average of
18.5 and 31.0 polymorphic markers per primer pair in Upland and Pima cotton, respectively. Using several restriction enzyme
combinations before pre-selective amplification in combination with various frequent 4 bp-cutters or 6 bp-cutters after selective
amplification, the pooled AFLP and cAFLP will provide unlimited number of polymorphic markers for genome-wide mapping and
fingerprinting. 相似文献
6.
用SSR和AFLP技术分析花生抗青枯病种质遗传多样性的比较 总被引:10,自引:0,他引:10
由Ralstonia solanacearum E.F.Smith引起的青枯病是若干亚洲和非洲国家花生生产的重要限制因子,利用抗病品种是防治这一病害最好的措施。虽然一大批抗青枯病花生种质资源材料已被鉴定出来,但对其遗传多样性没有足够的研究,限制了在育种中的有效利用。本研究以31份对青枯病具有不同抗性的栽培种花生种质为材料,通过简单序列重复(SSR)和扩增片段长度多态性(AFLP)技术分析了它们的遗传多样性。通过78对SSR引物和126对AFLP引物的鉴定,筛选出能显示抗青枯病种质多态性的SSR引物29对和AFLP引物32对。所选用的29对多态性SSR引物共扩增91条多态性带,平均每对引物扩增3.14条多态性带;32对多态性AFLP引物共扩增72条多态性带,平均扩增2.25条多态性带。在所筛选引物中,4对SSR引物(14H06,7G02,3A8,16C6)和1对AFLP引物(P1M62)检测花生多态性的效果优于其他引物。SSR分析获得的31个花生种质的遗传距离为0.12-0.94,平均为0.53,而AFLP分析获得的遗传距离为0.06~0.57,平均为0.25,基于SSR分析的遗传距离大于基于AFLP分析的遗传距离,疏枝亚种组的遗传分化相对大于密枝亚种组。基于两种分析方法所获得的聚类结果基本一致,但SSR数据聚类结果与栽培种花生的形态分类系统更为吻合。根据分析结果,对构建青枯病抗性遗传图谱群体的核心亲本和抗性育种策略提出了建议。 相似文献
7.
L.-H. Zhang P. Ozias-Akins G. Kochert S. Kresovich R. Dean W. Hanna 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(6-7):895-902
Bermudagrasses (Cynodon spp.) are major turfgrasses for home lawns, public parks, golf courses and sport fields, and are widely adapted to tropical
and warmer temperate climates. Morphological and physiological characteristics are not sufficient to differentiate some bermudagrass
genotypes because the differences between them are often subtle and subject to environmental influence. In this study, a DNA-typing
technique, amplified fragment length polymorphism (AFLP), was used to differentiate bermudagrass genotypes and to explore
their genetic relationships. Twenty seven bermudagrass cultivars and introductions, mostly from the Coastal Plain Experiment
Station in Tifton, Ga., were assayed by the radioactive (32P) and the fluorescence-labeled AFLP methods. The AFLP technique produced enough polymorphism to differentiate all 27 bermudagrass
genotypes, even the closely related ones. An average of 48–74 bands in the 30–600-bp size range was detected by the 32P-labeled AFLP method. The results indicated that most of the 14 primer combinations tested in this study could be used to
distinguish bermudagrass genotypes, and that some single primer-pairs could differentiate all 27 of them. To test the reliability
and reproducibility of the AFLP procedure, three DNA isolations (replications) of the 27 bermudagrass genotypes were assayed
using five primer pairs. Only 0.6% of the bands were evaluated differently among the three replications. One replication of
one genotype (which was most likely a planting contaminant) was grouped in an unexpected cluster using the Unweighted Pair
Group Mean Average (UPGMA) method. A one- or two-band difference in scoring did not change the clustering of genotypes or
the replications within genotypes. The 27 genotypes were grouped into three major clusters, many of which were in agreement
with known pedigrees. Trees constructed with different primer combinations using 32P- and fluorescence-labelling formed similar major groupings. The semi-automated fluorescence-based AFLP technique offered
significant improvements on fragment sizing and data handling. It was also more accurate for detection and more efficient
than the radioactive labelling method. This study shows that the AFLP technique is a reliable tool for differentiating bermudagrass
genotypes and for determining genetic relationships among them.
Received: 28 July 1998 / Accepted: 3 November 1998 相似文献
8.
Data on genetic similarity among crop cultivars is of vital importance for the plant breeder. The objectives of this study
were to group pepper (Capsicum annuum L.) genotypes into clusters according to their distances as estimated by morphological traits and amplified fragment length
polymorphism (AFLP) markers and to assess the relationships between the two. Thirty-nine pepper genotypes obtained from different
countries were grown in the greenhouse at University of the Free State, South Africa, during 2001 and 2002 in a randomized
complete block design with three replications. A total of 20 different morphological traits were measured and six AFLP primer
pairs were used to estimate pairwise genetic distances. Both datasets showed high genetic distances among the different genotypes,
indicating high genetic diversity among them. The mean genetic distance among Ethiopian pungent elongated-fruit genotypes,
was lower than that between them and the introduced ones. Morphological and AFLP distance estimations generally clustered
together genotypes with similar fruit sizes. Significant, positive correlation was observed between morphological and AFLP
diversity estimations. The narrow genetic basis among the Ethiopian pungent elongated-fruit cultivars suggests that the pepper
breeding program of Ethiopia should focus on enriching its germplasm through local collection and introductions from other
parts of the world. 相似文献
9.
Jian Wei Sun Min De Jin Chun Jiang Zhou Qing Kai Yang Man Li Weng Lin De Duan Pu Xu Jia Hai Ma Bin Wang 《Plant Molecular Biology Reporter》2005,23(3):251-262
Twenty-sevenPorphyra lines from 5 classes, including lines widely used in China, wild lines, and lines introduced to China from abroad in recent
years, were screened by means of amplified fragment length polymorphism (AFLP) with 24 primer pairs. From the generated AFLP
products, 13 bands that showed stable and repeatable AFLP patterns amplified by primer pairs M-CGA/E-AA and M-CGA/E-TA were
scored and used to develop the DNA fingerprints of the 27Porphyra lines. Moreover, the DNA fingerprinting patterns were converted into computer language expressed with digitals 1 and 0, which
represented the presence (numbered as 1) or absence (numbered as 0) of the corresponding band. On the basis of these results,
computerized AFLP DNA fingerprints were constructed in which each of the 27Porphyra lines has its unique AFLP fingerprinting pattern and can be easily distinguished from others. Software called PGI-AFLP (Porphyra germ-plasm identification-AFLP) was designed for identification of the 27Porphyra lines. In addition, 21 specific AFLP markers from 15Porphyra lines were identified; 6 AFLP markers from 4Porphyra lines were sequenced, and 2 of them were successfully converted into SCAR (sequence characterized amplification region) markers.
The developed AFLP DNA fingerprinting and specific molecular markers provide useful ways for the identification, classification,
and resource protection of thePorphyra lines. 相似文献
10.
AFLP-based genetic diversity assessment amongst agronomically important natural and some newly synthesized lines of Brassica juncea 总被引:1,自引:0,他引:1
A. Srivastava V. Gupta D. Pental A. K. Pradhan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,102(2-3):193-199
AFLP markers were employed to assess the genetic diversity amongst 21 established natural and nine synthetic varietes and
lines of Brassica juncea originating from Asia, Australia, Canada, Eastern Europe and Russia. Six of the synthetics used for diversity studies have
been developed recently. Twenty one EcoRI/MseI-based AFLP primer pairs generated a total of 1251 scorable fragments among the 30 genotypes studied, of which 778 bands
were polymorphic with an average of 37 polymorphic bands per primer pair. On the basis of the similarity coefficients (F value), cluster analysis was performed using the UPGMA method. The 30 B. juncea lines could be grouped into three distinct clusters. All the Indian, Chinese and previously developed synthetics formed one
cluster (cluster A), the recently developed synthetics formed a separate cluster (cluster B) and the lines from Australia,
Canada, Eastern Europe and Russia formed the third cluster (cluster C). A majority of the lines were uniquely identified by
one or more primer pairs due to the presence or absence of variety specific band(s). Four primer pairs were found to be most
informative, since these uniquely identified all the genotypes assayed. These four primer pairs, could therefore be used as
fingerprinting primers for varietal identification.
Received: 1 November 1999 / Accepted: 8 May 2000 相似文献
11.
Enrico Noli Maria Soccorsa Teriaca Sergio Conti 《Molecular breeding : new strategies in plant improvement》2012,29(3):687-698
Essentially derived varieties (EDV) are considered to be those that are obtained from an original variety by means of methods
that do not substantially modify its genetic structure, and whose characteristics are therefore derived from the original.
The identification of EDV requires the definition of a threshold value of genetic similarity between the new and the original
variety that if exceeded would suggest derivation. Although protocols for estimating genetic similarities based on molecular
markers have been proposed for EDV identification in some species, no information was available for durum wheat (Triticum durum). A set of 60 genotypes (F8 or F9 lines and their parents) representing different levels of relatedness were profiled using
14 (later reduced to 13 by excluding the most deviant one) amplified fragment length polymorphism (AFLP) primer combinations
and 109 simple sequence repeat (SSR) loci evenly distributed in the genome. For both marker types, an EDV threshold was calculated
according to the “tail principle” on the distributions of Jaccard similarities among the subset of 39 independent genotypes.
For all pairs of closely related lines branched in advanced generations (F7–F8), or for all but one pairs of lines deriving
from the same F4 family, similarities exceeded the thresholds for both marker types, indicating a very good agreement in showing
cases of suspected derivation. Compared to SSR markers, AFLP markers appear more suitable for assessing essential derivation
because of their superior cost-efficiency. Based on 13 AFLP primer combinations, a threshold of 0.96 Jaccard similarity is
proposed, below which a variety should be considered to be independently derived. 相似文献
12.
DNA fingerprinting of Cryptosporidium parvum isolates using amplified fragment length polymorphism (AFLP) 总被引:3,自引:0,他引:3
Blears MJ Pokorny NJ Carreno RA Chen S De Grandis SA Lee H Trevors JT 《The Journal of parasitology》2000,86(4):838-841
The genetic variability of 10 Cryptosporidium parvum isolates of human and animal origin was investigated using amplified fragment length polymorphism (AFLP). Analysis of fluorescent dye-labeled amplified products was carried out using an ABI PRISMS 377 DNA sequencer and ABI PRISMS GeneScan software. One-hundred and twelve primer combinations were evaluated using a single C. parvum isolate. The patterns generated were highly reproducible. For subsequent study, a subset of 9 primer pairs that yielded 30-90 DNA fragments after the polymerase chain reaction, within the size range of 50-500 bp, was used to screen the 10 C. parvum isolates, including 7 bovine, 1 equine, and 2 of human origin. The animal isolates produced identical fingerprint patterns with every primer combination tested. Of the 2 human isolates tested, 1 of the isolates, passaged in calves, generated the same AFLP DNA banding patterns as the animal isolates, whereas the other isolate, obtained directly from human feces, produced unique patterns. Polymorphism, detected by comparison of the fingerprint patterns of the latter human isolate with the common pattern shared by all other isolates, ranged from 17 to 35% for the 9 primer pairs. The results show that AFLP is a useful method for differentiating C. parvum isolates into 2 distinct genotypes. 相似文献
13.
羊草种质基因组DNA的AFLP多态性研究 总被引:15,自引:0,他引:15
羊草是禾本科牧草之王 ,在当前我国西部生态建设和草原畜牧业发展中发挥着重要作用。用AFLP方法对2 7份我国不同地区分布的羊草 (Leymuschinensis (Trin .)Tzvel)材料进行了基因组DNA多态性分析 ,8对AFLP引物组合在 2 7个不同羊草基因型中共扩增出 5 37条带 ,产生出的DNA片段大小分布在 75bp - 5 30bp之间。其中单态性带 89条 ,占 16 .6 % ,多态性带 32 9条 ,占 6 1.3%。平均每对引物组合扩增的DNA带数为 6 6 .13,总的多态性比率为 78.84%。AFLP多态信息含量PIC值分布于 0 .0 - 0 .5之间 ,平均PIC值为 0 .2 16 ,出现的PIC最大值 (0 .5 )约占AFLP标记的 8.5 % ,说明羊草基因组DNA的多态性比较丰富。以 5 37个AFLP标记为原始数据 ,根据Nei和Li的方法对 2 7份羊草材料进行遗传变异和聚类分析的结果表明 :羊草种内有高频率的遗传变异发生 ,且与地理分布和生态环境密切相关 ;2 7份羊草不同基因型被划分为四大类群 ,不同类群相互间的遗传距离相对较大 ,在树状图中表现为较远的亲缘关系。对羊草种内遗传变异发生的原因和品种的形成进行了初步讨论。 相似文献
14.
利用AFLP技术对26个竹子种类进行了多样性分析, 以探索引物组合数量对准确研究竹子类群系统关系的影响。实验共随机选取10对AFLP引物, 并对所得10组AFLP标记数据随机组合后进行Nei氏遗传距离/UPGMA聚类分析。每对AFLP引物
扩增数据为一组, 随着用于聚类统计的AFLP标记数据随机组合数量的增加, 26个竹子种类的聚类关系趋向一致。这提示我们,在系统学研究中, 足够数量的引物组合是获得供试材料间准确聚类关系的基础, 应采用对各AFLP引物组合数据随机累加后进行聚类分析的方法, 以聚类关系为标准来确定用于分析供试品种的最少引物组合数量。 相似文献
15.
To validate strain typing by amplified fragment length polymorphism (AFLP) analysis in shiitake (Lentinula edodes) cultivars, the reproducibility of AFLP markers with DNA extracted from the heat-dried fruiting body was evaluated. DNAs were extracted from three different portions of the heat-dried fruiting body – the stipe, pileus, and gill – and AFLP analysis of all parts was carried out using two combinations of selected amplification primer pairs. AFLP profiles of DNA from the gill tissue of heat-dried fruiting body were almost identical to those of cultured mycelia in the same strains, although it was difficult to detect reproducible AFLP profiles from stipe and pileus DNA. These results indicated that AFLP analysis would be applicable for strain typing with heat-dried fruiting bodies of L. edodes by using the DNA extracted from gills.Contribution No. 364 of the Tottori Mycological Institute 相似文献
16.
P. F. Bert G. Charmet P. Sourdille M. D. Hayward F. Balfourier 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(3-4):445-452
AFLP markers have been successfully employed for the development of a high-density linkage map of ryegrass (Lolium
perenne L.) using a progeny set of 95 plants from a testcross involving a doubled-haploid tester. This genetic map covered 930 cM
in seven linkage groups and was based on 463 amplified fragment length polymorphism (AFLP) markers using 17 primer pairs,
three isozymes and five EST markers. The average density of markers was approximately 1 per 2.0 cM. However, strong clustering
of AFLP markers was observed at putative centromeric regions. Around these regions, 272 markers covered about 137 cM whereas
the remaining 199 markers covered approximately 793 cM. Most genetic distances between consecutive pairs of markers were smaller
than 20 cM except for five gaps on groups A, C, D, F and G. A skeletal map with a uniform distribution of markers can be extracted
from this high-density map, and can be applied to detect and map QTLs. We report here the application of AFLP markers to genome
mapping, in Lolium as a prelude to quantitative trait locus (QTL) identification for diverse agronomic traits in ryegrass and for marker-assisted
plant breeding.
Received: 4 November 1998 / Accepted:15 March 1999 相似文献
17.
M. A. Filjushin O. A. Kholda E. Z. Kochieva N. N. Ryzhova 《Russian Journal of Genetics》2011,47(4):492-496
The results of the AFLP analysis of 16 leek (Allium porrum) accessions and related species of the sections of the genus Allium are presented. Restriction enzymes and primer combinations for the identification of the genotypes of the A. porrum accessions were chosen. As a result, 265 polymorphic AFLP fragments were amplified for 25 analyzed genotypes, and specific
spectra of DNA fragments were obtained for each accession. A total of 24 fragments specific for the A. porrum genome was detected, of which only two characterized the genotypes of individual accessions. A wide range of genetic diversity
(0.11–0.32) was revealed for the A. porrum varieties and lines used in the analysis. The highest level of similarity in the analyzed set of accessions was found between
A. porrum and sand leek (A. scorodoprasum). 相似文献
18.
Mukesh K. Dubey Ajit K. Shasany Omp. Dhawan Ashutosh K. Shukla Suman P. S. Khanuja 《Journal of genetics》2010,89(1):9-19
Downy mildew (DM) caused by Peronospora arborescens, is a serious disease in opium poppy (Papaver somniferum), which has a world-wide spread. The establishment of DM-resistant cultivars appears to be a sustainable way to control the
disease. In this paper, we present the results of a study aimed at the identification of amplified fragment length polymorphism
(AFLP) markers for DM-resistance in opium poppy. Three opium poppy genotypes (inbred over about 10 years): Pps-1 (DM-resistant),
Jawahar-16 (DM-susceptible) and H-9 (DM-susceptible) were crossed in a diallel manner and the F1 progeny along with the parents
were subjected to AFLP analysis of chloroplast (cp) and nuclear DNA with seven and nine EcoRI / MseI primer combinations, respectively. cpDNA AFLP analysis identified 24 Pps-1 (DM-resistant)-specific unique fragments that
were found to be maternally inherited in both the crosses, Pps-1 × Jawahar-16 and Pps-1 × H-9. In the case of nuclear DNA
AFLP analysis, it was found that 17 fragments inherited from Pps-1 were common to the reciprocal crosses of both (i) Pps-1
and Jawahar-16 as well as (ii) Pps-1 and H-9. This is the first molecular investigation on the identification of polymorphism
between DM-resistant and DM-susceptible opium poppy genotypes and development of DM-resistant opium poppy genotype-specific
AFLP markers. These AFLP markers could be used in future genetic studies for analysis of linkage to the downy mildew resistance
trait. 相似文献
19.
Both morphological characteristics and amplified fragment length polymorphism (AFLP) markers were used to validate the genetic
fidelity of 1 080 field-grown Echinacea purpurea plants regenerated from leaf explants of donor T5-9. Morphological diagnosis revealed that 1 067 out of 1 080 regenerants
were normal, while 13 regenerants were aberrant. AFLP analysis was further performed to assess DNA variations among donor,
43 sampled normal regenerants and all 13 aberrant regenerants. Seven primer combinations generated 471 fragments among donor
and normal regenerants, of which 9 fragments were polymorphic. The same primer pairs generated 484 fragments for aberrant
regenerants, of which 417 fragments were polymorphic. UPGMA clustering indicated that 42 normal regenerants and donor fell
into same cluster at similarity scale of > 0.99, while all 13 aberrant regenerants and one morphologically normal regenerant
comprised the other clusters. AFLP analysis indicated that these 14 regenerants are off-types. 相似文献
20.
Amplified Fragment Length Polymorphism (AFLP) as a source of genetic markers for red algae 总被引:7,自引:0,他引:7
A recent PCR-based fingerprinting technique, amplified fragment length polymorphism (AFLP), was successfully applied to the
red alga Chondrus crispus Stackh. This is apparently the first account to describe the application of AFLP methodology to
an alga. Six isolates of C. crispus were analyzed by AFLP. A total of twenty-five primer pairs were screened and six primer
pairs were selected for further investigation. Both conservative and variable markers were identified within and between populations;
some markers were unique to individuals. As such, AFLP should prove useful as a source of genetic markers in algae for applications
as diverse as genome mapping to population genetic investigations.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献