共查询到20条相似文献，搜索用时 15 毫秒
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Kaushik Ghosal Scott P. Naples Alexander R. Rabe Kathleen A. Killian 《Archives of insect biochemistry and physiology》2010,74(1):38-51
Immediate early genes (IEG) such as c‐Fos and Fos‐related antigens (FRA) have been used as markers of neuronal activation. In this study, we determined whether the expression of c‐Fos/FRAs is increased in the brains of adult male Acheta domesticus crickets following agonistic interactions. We looked for c‐Fos/FRA proteins in the brain of un‐fought, control male crickets and of dominant and subordinate male crickets sacrificed at different time periods following an agonistic interaction. Using immunoblot analysis, we found four different c‐Fos/FRA‐like proteins in the adult cricket brain. Continuous agonistic interaction increased c‐Fos/FRA protein expression in the brains of subordinate males compared to control and dominant males. In addition, direct electrical stimulation of the male cricket antennae increased c‐Fos/FRA‐like protein in the brain. We identified the specific brain regions that exhibit c‐Fos/FRA‐like immunoreactivity in crickets. We detected c‐Fos/FRA‐like cellular immunoreactivity in different functional regions of the adult brain including the pars intercerebralis, protocerebrum, deutocerebrum, and the cortex of the mushroom bodies. © 2010 Wiley Periodicals, Inc. 相似文献
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This study investigated sex‐specific effects of repeated stress and food restriction on food intake, body weight, corticosterone plasma levels and expression of corticotropin‐releasing factor (CRF) in the hypothalamus and relaxin‐3 in the nucleus incertus (NI). The CRF and relaxin‐3 expression is affected by stress, and these neuropeptides produce opposite effects on feeding (anorexigenic and orexigenic, respectively), but sex‐specific regulation of CRF and relaxin‐3 by chronic stress is not fully understood. Male and female rats were fed ad libitum chow (AC) or ad libitum chow and intermittent palatable liquid Ensure without food restriction (ACE), or combined with repeated food restriction (60% chow, 2 days per week; RCE). Half of the rats were submitted to 1‐h restraint stress once a week. In total, seven weekly cycles were applied. The body weight of the RCE stressed male rats significantly decreased, whereas the body weight of the RCE stressed female rats significantly increased compared with the respective control groups. The stressed female RCE rats considerably overate chow during recovery from stress and food restriction. The RCE female rats showed elevated plasma corticosterone levels and low expression of CRF mRNA in the paraventricular hypothalamic nucleus but not in the medial preoptic area. The NI expression of relaxin‐3 mRNA was significantly higher in the stressed RCE female rats compared with other groups. An increase in the expression of orexigenic relaxin‐3 and misbalanced hypothalamic‐pituitary‐adrenal axis activity may contribute to the overeating and increased body weight seen in chronically stressed and repeatedly food‐restricted female rats . 相似文献
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V. Reghunandanan R. D. Badgaiyan R. K. Marya R. Reghunandanan B. K. Maini 《Chronobiology international》1989,6(2):123-129
Effect of lithium injections through chronically implanted cannulae into the bilateral suprachiasmatic nuclei (SCN) on the circadian rhythm of food intake was investigated in the rat. It was observed that the circadian rhythm was disrupted by injections of lithium at the beginning of the light as well as the dark phase of the LD cycle. In either case the percentage of the food consumed during the 12-hr light period increased while that during the dark period decreased without any significant change in the total daily intake. Disruptions in the circadian rhythm of food intake failed to show any dose-response relation. Injections of saline into the SCN or lithium into the nearby SCN area did not produce a disruption of the circadian rhythm of food intake. 相似文献
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J. Peter H. Burbach Roger A. H. Adan Stephen J. Lolait Fred W. van Leeuwen Eva Mezey Miklos Palkovits Claude Barberis 《Cellular and molecular neurobiology》1995,15(5):573-595
Summary 1. VP and OT mediate their wealth of effects via 4 receptor subtypes V1a, V1b, V2, and OT receptors.2. We here review recent insights in the pharmacological properties, structure activity relationships, species differences in ligand specificity, expression patterns, and signal transduction of VP/OT receptor.3. Furthermore, the existence of additional VP/OT receptor subtypes is discussed. 相似文献
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Bingjin Li Katsuya Suemaru Yoshihisa Kitamura Yutaka Gomita Hiroaki Araki Ranji Cui 《Journal of biochemical and molecular toxicology》2013,27(11):486-491
Previous studies have shown that the antidepressive‐like effect of tricyclic antidepressants is blocked by repeated treatments with adrenocorticotropic hormone (ACTH). However, little is known about the neuroanatomy underlying the mechanism of the imipramine treatment‐resistant depression model. In the present study, first experimental evidence showed no significant difference of the serum imipramine concentrations between the saline and ACTH‐treated rats. In further study, imipramine produced significant increases in the c‐Fos expression in the medial prefrontal cortex (mPFC), the dentate gyrus of the hippocampus (DGH), and the central nucleus of the amygdala (CeA), in rats repeatedly treated with saline. The imipramine‐increased c‐Fos immunoreactivity was suppressed in the mPFC of rats repeatedly treated with ACTH. However, there was no significant difference in c‐Fos expression in the DGH and CeA between ACTH‐ and saline‐treated rats. These results suggest that the mPFC is maybe involved in effects of the imipramine in the ACTH‐treated rats. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:486‐491, 2013; View this article online at wileyonlinelibrary.com . DOI 10.1002/jbt.21510 相似文献
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Ildik Rcz Astrid Markert Daniela Mauer Birgit Stoffel‐Wagner Andreas Zimmer 《Addiction biology》2013,18(4):678-688
The brain stress‐response system is critically involved in the addiction process, stimulating drug consumption and the relapse to drug taking in abstinent addicts. At the same time, its functioning is affected by chronic drug exposure. Here, we have investigated the role of the endogenous opioid peptide dynorphin as a modulator of effects of long‐term ethanol consumption on the brain stress‐response system. Using the two‐bottle choice paradigm, we demonstrate an enhanced ethanol preference in male dynorphin knockout mice. Exposure to mild foot shock increased ethanol consumption in wild‐type control littermates, but not in dynorphin‐deficient animals. Blood adrenocorticotropic hormone levels determined 5 minutes after the shock were not affected by the genotype. We also determined the neuronal reactivity after foot shock exposure using c‐Fos immunoreactivity in limbic structures. This was strongly influenced by both genotype and chronic ethanol consumption. Long‐term alcohol exposure elevated the foot shock‐induced c‐Fos expression in the basolateral amygdala in wild‐type animals, but had the opposite effect in dynorphin‐deficient mice. An altered c‐Fos reactivity was also found in the periventricular nucleus, the thalamus and the hippocampus of dynorphin knockouts. Together these data suggest that dynorphin plays an important role in the modulation of the brain stress‐response systems after chronic ethanol exposure. 相似文献
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Plasma ACTH and/or corticosterone levels were measured in conscious rats 30 min after subcutaneous administration of arginine vasopressin (AVP), oxytocin (OT) and various analogs with a large range of activity on the vasopressor (V1), antidiuretic (V2) or oxytocic receptors. The comparison of their dose-response curves indicated that two different mechanisms are involved in the release of ACTH by neurohypophysial peptides and their analogs. AVP itself and a specific vasopressor agonist (Phe2, Orn8, OT) displayed a similar, high slope dose-response curve. Non-vasopressor analogs, such as dDAVP were characterized by a low slope dose-response curve. Furthermore, dDAVP potentiated CRF and neither its own ACTH-releasing action nor its potentiation of CRF were sensitive to previous VI- or V2-receptor blockade. These results, together with other available data, are interpreted as indicative of the existence of two mechanisms of action for ACTH release by AVP and its analogs in vivo: an indirect action via endogenous CRF release, mediated by a VI receptor mechanism, and a direct action on the pituitary, shared by dDAVP and other non-vasopressor analogs, with receptor characteristics different to both the V1 and the V2 classical types. 相似文献
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Ji‐Jun Zhao Zhao‐Feng Wu Ying‐Hao Yu Ling Wang Li Cheng 《Journal of cellular physiology》2018,233(9):7182-7194
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《Animal cells and systems.》2012,16(2):157-161
Mice homozygous for the lethal autosomal recessive anorexia mutation (anx) present with premature death around postnatal day 22. The anorexia mutant mice also present phenotypes such as reduced body weight, decreased food intake, and abnormal behavior characteristics such as body tremors, hyperactivity, uncoordinated gait, and head weaving. In order to investigate the expression of c‐Fos in the hippocampus of anorexia mutant mice, the immunohistochemistry was performed in this study. The anorexia mutant mice exhibited lower expression of c‐Fos in the hippocampus regions than the control group. In the CA3 and dentate gyrus, the number of c‐Fos‐positive cells in anorexia mutant mice was noticeably lower than that in control mice. However, no significant difference was found in the number of c‐Fos‐positive cells in CA1 of the two groups. The result suggests that the phenotypic characteristics of anorexia mutant mice may be associated with the hippocampal deficits of c‐Fos expression. 相似文献
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Helen D. Nicholson R.W. Swann G.D. Burford D.Claire Wathes D.G. Porter B.T. Pickering 《Regulatory peptides》1984,8(2):141-146
Oxytocin, vasopressin and neurophysin-like immunoreactivity have been identified and measured by radioimmunoassay in extracts of human and rat testis and human fetal adrenal tissue. The authenticity of these polypeptides has been confirmed by their behaviour on high performance liquid chromatography. The concentrations of the hormone were too great to be explained by known circulating levels of the polypeptides, and their presence in steroid secreting organs suggests a possible role for them in steroidogenesis. The peptides may be taken up and concentrated by the tissues but the co-localisation of neurophysins with the hormones points towards local synthesis. 相似文献
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脑室内注射一氧化氮供体及一氧化氮合酶抑制剂对室旁核大细胞自发电活动的作用 总被引：5，自引：0，他引：5
在乌拉坦麻醉的成年ＳＤ大鼠上,用玻璃微电极细胞外记录的方法,观察了脑室内注射一氧化氮供体及一氧化氮合酶抑制剂对室旁核大细胞自发电活动的作用。结果发现：脑室内注射一氧化氮供体硝普钠对下丘脑室旁核中的加压素神经元产生剂量依赖性抑制作用;脑室内注射一氧化氮合酶抑制剂对加压素神经元也产生抑制作用。上述两种药物对催产素神经元均无作用。这些结果提示：一氧化氮可能在调节加压素和催产素神经元活动中起着不同的作用。 相似文献
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用细胞外记录的方法研究了猕猴在执行图形辨认引导的有序运动（ＦＲＳ）时大脑皮层弓形沟距周围背外侧运动前皮层（ＰＭｄ）Ｆ２区和腹外侧运动前皮层（ＰＭｖ）Ｆ４区的放电活动在ＦＲＳ的暗示期,Ｆ２和Ｆ４区中分别有５２％（３９／７５）和１６．９％（１３／７７）的细胞发生放电变化;在触摸反应期,各有５１％（３８／７５）和８７％（６７／７７）的细胞发生放电变化,经统计检验,均有显著差异。Ｆ２区比Ｆ４区有更多细胞对 相似文献
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Physiological dependence and associated withdrawal episodes are thought to constitute a motivational force that sustains alcohol use and abuse and may contribute to relapse in dependent individuals. Although no animal model duplicates alcoholism, models for specific factors, like withdrawal, are useful for identifying potential genetic and neural determinants of liability in humans. Previously, we identified a quantitative trait locus (QTL) and gene (Mpdz, which encodes the multi‐PDZ domain protein) on chromosome 4 with a large effect on alcohol withdrawal in mice. Using congenic mice that confirm this QTL and c‐Fos expression as a high‐resolution marker of neuronal activation, we report that congenic mice show significantly less neuronal activity associated with alcohol withdrawal in the rostroventral caudate putamen (rvCP), but not other parts of the striatum, compared with background strain mice. Moreover, bilateral rvCP lesions significantly increase alcohol withdrawal severity. Using retrograde (fluorogold) and anterograde (Texas Red conjugated dextran amine) tract tracing, we found that ～25% of c‐Fos immunoreactive rvCP neurons project to caudolateral substantia nigra pars reticulata (clSNr), which we previously found is crucially involved in withdrawal following acute and repeated alcohol exposure. Our results expand upon work suggesting that this QTL impacts alcohol withdrawal via basal ganglia circuitry associated with limbic function, and indicate that an rvCP‐clSNr projection plays a critical role. Given the growing body of evidence that the syntenic region of human chromosome 9p and human MPDZ gene are associated with alcohol abuse, our results may facilitate research on alcohol dependence and associated withdrawal in clinical populations. 相似文献