植物的功能基因组学研究进展

基因组研究计划包括以全基因组测序为目标的结构基因组学和以基因功能鉴定为目标的功能基因组学两方面的内容。目前基因功能鉴定的方法主要有：基因表达的系统分析(SAGE)、cDNA微阵列、DNA(基因)芯片、蛋白组技术以及基于转座子标签和T_DNA标签的反求遗传学技术等。本文对上述各种技术的优缺点以及它们在植物基因功能鉴定中的应用进行了综述。 Abstract: The genome projects comprise the structural genomics focusing on determining the complete sequences of the genome and the functional genomics focusing on elucidating the biological function of genes.The rapidly evolving tools for functional genomics research include Serial Analysis of Gene Expression (SAGE),cDNA microarray,DNA (or gene) chips,proteome project and the reverse genetics technique based on the well-established transposon tagging and T?DNA tagging systems.In this paper,the advantages and disadvantages of such techniques and application of these techniques in plant functional genomics research are reviewed and future prospective are also presented.     

Special Issue on “Plant Genomics”

正We are very pleased to announce a special issue,to be published in the spring of 2019,on"Plant Genomics"in the journal Genomics,ProteomicsBioinformatics(GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions.The multiple released genomes further promote the research on genome evolution and its functional     

Special Issue on “Plant Genomics”

正We are very pleased to announce a special issue,to be published in Summer 2018,on "Plant Genomics" in the journal Genomics,ProteomicsBioinformatics(GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions.The multiple released genomes further promote the research on genome evolution and its functional     

Special Issue on “Plant Genomics”

正We are very pleased to announce a special issue,to be published in the spring of 2019,on‘‘Plant Genomics’’in the journal Genomics,ProteomicsBioinformatics(GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions.The multiple released genomes further promote the research on genome evolution and its functional     

Special Issue on “Plant Genomics”

正We are very pleased to announce a special issue,to be published in the fall of 2018,on‘‘Plant Genomics’’in the journal Genomics,ProteomicsBioinformatics(GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions.The multiple released genomes further promote the research on genome evolution and its functional     

Special Issue on ‘‘Plant Genomics”

正We are very pleased to announce a special issue, to be published in the summer of 2019,on ‘‘Plant Genomics’’ in the journal Genomics, ProteomicsBioinformatics (GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions. The multiple released genomes further promote the research on genome evolution and its functional     

Special Issue on “Plant Genomics”

<正>We are very pleased to announce a special issue,to be published in Summer 2018,on"Plant Genomics"in the journal Genomics,ProteomicsBioinformatics(GPB).The development of genomics has greatly accelerated and even renovated the plant research.The sequencing of plant genomes has greatly facilitated the research on gene functions.The multiple released genomes further promote the research on genome evolution and its functional relevance.Meanwhile,the state-of-art epigenetics studies go on a genome-wide level.All these     

Targeted Mutagenesis of Duplicated Genes in Caenorhabditis elegans Using CRISPR-Cas9

正CRISPR(clustered regularly interspaced short palindromic repeats)-Cas9-based genome editing has revolutionized functional genomics in many biological research fields.The specificity and potency of CRISPR-Cas9 genome editing make it ideal for investigating the function of genes in vivo(Hsu     

Microarray and proteomic analysis of brassinosteroid- and gibberellin-regulated gene and protein expression in rice

Brassinosteroid (BR) and gibberellin (GA) are two groups of plant growth regulators essential for normal plant growth and development. To gain insight into the molecular mechanism by which BR and GA regulate the growth and development of plants, especially the monocot plant rice, it is necessary to identify and analyze more genes and proteins that are regulated by them. With the availability of draft sequences of two major types, japonica and indica rice, it has become possible to analyze expression changes of genes and proteins at genome scale. In this review, we summarize rice functional genomic research by using microarray and proteomic approaches and our recent research results focusing on the comparison of cDNA microarray and proteomic analyses of BR- and GA-regulated gene and protein expression in rice. We believe our findings have important implications for understanding the mechanism by which BR and GA regulate the growth and development of rice.     

Microarray,SAGE and their applications to cardiovascular diseases

The wealth of DNA data generated by the human genome project coupling with recently invented high-throughput gene expression profiling techniques has dramatically sped up the process for biomedical researchers on elucidating the role of genes in human diseases. One powerful method to reveal insight into gene functions is the systematic analysis of gene expression. Two popular high-throughput gene expression technologies, microarray and Serial Analysis of Gene Expression (SAGE) are capable of producing large amounts of gene expression data with the potential of providing novel insights into fundamental disease processes, especially complex syndromes such as cardiovascular disease, whose etiologies are due to multiple genetic factors and their interplay with the environment. Microarray and SAGE have already been used to examine gene expression patterns of cell-culture, animal and human tissues models of cardiovascular diseases. In this review, we will first give a brief introduction of microarray and SAGE     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics (TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and     

Triticeae Genomics and Genetics(TGG)

正Triticeae Genomics and Genetics(ISSN 1925-203X)is committed to publishing and disseminating significant original achievements in the related research fields of Triticeae genomics and Triticeae genetics.All papers chosen for publication should be innovative research work in the field of Triticeae genomics and Triticeae genetics,including classical genetics analysis,structural and functional analysis of Triticeae genome,isolation and