共查询到20条相似文献,搜索用时 31 毫秒
1.
V F Castellucci W N Frost P Goelet P G Montarolo S Schacher J A Morgan H Blumenfeld E R Kandel 《Journal de physiologie》1986,81(4):349-357
We have found that one cellular locus for the storage of the memory underlying short-term sensitization of the gill and siphon withdrawal reflex in Aplysia is the set of monosynaptic connections between the siphon sensory cells and the gill and siphon motor neurons. These connections also participate in the storage of memory underlying long-term sensitization. In animals that have undergone long-term sensitization, the amplitudes of the monosynaptic connections are significantly larger (2.2x) than the ones in control animals. To study the mechanisms of onset and retention of long-term synaptic facilitation that underly long-term sensitization and the role of protein synthesis in long-term memory, we have developed two types of reduced preparations: the intact reflex isolated from the remainder of the animal, and a dissociated cell culture system in which the monosynaptic component (sensory neurons and motor neurons) of the neuronal circuit mediating the withdrawal reflex is reconstituted. We found that protein synthesis inhibitors, such as anisomycin or emetine, and RNA synthesis inhibitors, such as actinomycin D or alpha-amanitin, blocked long-term facilitation without interfering with short-term facilitation. These results suggest that the acquisition of long-term memory may require the expression of genes and the synthesis of proteins not needed for short-term memory. 相似文献
2.
Thomson AM Bannister AP Mercer A Morris OT 《Philosophical transactions of the Royal Society of London. Series B, Biological sciences》2002,357(1428):1781-1791
We attempt to summarize the properties of cortical synaptic connections and the precision with which they select their targets in the context of information processing in cortical circuits. High-frequency presynaptic bursts result in rapidly depressing responses at most inputs onto spiny cells and onto some interneurons. These 'phasic' connections detect novelty and changes in the firing rate, but report frequency of maintained activity poorly. By contrast, facilitating inputs to interneurons that target dendrites produce little or no response at low frequencies, but a facilitating-augmenting response to maintained firing. The neurons activated, the cells they in turn target and the properties of those synapses determine which parts of the circuit are recruited and in what temporal pattern. Inhibitory interneurons provide both temporal and spatial tuning. The 'forward' flow from layer-4 excitatory neurons to layer 3 and from 3 to 5 activates predominantly pyramids. 'Back' projections, from 3 to 4 and 5 to 3, do not activate excitatory cells, but target interneurons. Despite, therefore, an increasing complexity in the information integrated as it is processed through these layers, there is little 'contamination' by 'back' projections. That layer 6 acts both as a primary input layer feeding excitation 'forward' to excitatory cells in other layers and as a higher-order layer with more integrated response properties feeding inhibition to layer 4 is discussed. 相似文献
3.
Mechanosensory neurons which innervate the siphon and have their cell bodies in the LE cluster of the abdominal ganglion ofAplysia have revealed many cellular and molecular processes that may play general roles in learning and memory. It was initially
suggested that these cells are largely responsible for triggering the gill-withdrawal reflex evoked by weak siphon stimulation,
and that most of this effect is mediated by their monosynaptic connections to gill motor neurons. This implied a simple link
between plasticity at these synapses and modifications of the reflex during learning. We review more recent studies from several
laboratories showing that the LE cells are not activated by very weak tactile stimuli that elicit the gill-withdrawal reflex,
and that an unidentified population of siphon sensory neurons has lower mechanosensory thresholds and produces shorter latency
responses. Furthermore, the direct connections between LE cells and gill motor neurons make a minor contribution when the
reflex is elicited in pinned siphon preparations by light stimuli that weakly activate the LE cells. Because weak mechanical
stimulation of the unrestrained siphon causes little or no LE cell activation, it is unlikely that, under natural conditions,
sensitization or conditioning of reflex responses elicited by light siphon touch depends upon plasticity of LE cell synapses
onto either motor or interneurons.
The LE cells appear to function as nociceptors because they are tuned to noxious stimuli and, like mammalian nociceptors,
show peripheral sensitization following nociceptive activation. This sensitization and the profound activity-dependent potentiation
of LE synapses indicate that LE cell contributions to defensive reflexes should be largest during and after intense activation
of the LE cells by noxious stimulation (with the LE cell plasticity contributing to long-lasting memory of peripheral injury).
The LE sensory neurons offer special opportunities for direct tests of this and other hypotheses about specific mnemonic functions
of fundamental mechanisms of neural plasticity. 相似文献
4.
In the sea slug Aplysia, buccal synapses of cerebral-buccal interneurons (CBIs) CBI-2 and CBI-12 exhibit short-term synaptic enhancement (STE), including
frequency-dependant facilitation and augmentation/post-tetanic potentiation (AUG/PTP). The STE that results from driving CBI-2
or CBI-12 is associated with significantly decreased latency to burst onset in buccal premotor neurons and motor neurons,
increased cycle frequency of ingestion buccal motor programs (iBMPs) and increased intraburst firing frequency of buccal neurons
during iBMPs. Tests of paired-pulse facilitation during AUG/PTP suggest that the locus for this plasticity is presynaptic.
The AUG/PTP is not elicited by heterosynaptic pathways, indicating that its origin is homosynaptic. At low CBI-2 and CBI-12
firing frequencies, STE is likely to contribute to iBMP initiation, while at higher firing frequencies, STE is correlated
with increased cycle frequency of iBMPs. Thus, STE is an important component of the mechanisms whereby cerebral neurons regulate
cyclic feeding programs and likely contributes to observed variations in behavioral responses, including feeding arousal.
Electronic Publication 相似文献
5.
Short-term synaptic plasticity influences how presynaptic spike patterns control the firing of postsynaptic targets. Here we investigated whether specific mechanisms of short-term plasticity are regulated in a target-dependent manner by comparing synapses made by cerebellar granule cell parallel fibers onto Golgi cells (PF-->GC synapse) and Purkinje cells (PF-->PC synapse). Both synapses exhibited similar facilitation, suggesting that any differential short-term plasticity does not reflect differences in the initial release probability. PF-->PC synapses were highly sensitive to stimulus bursts, which could result in either depression of subsequent responses, mediated by endocannabinoid-dependent retrograde signaling, or enhancement of responses through posttetanic potentiation (PTP). In contrast, stimulus bursts had remarkably little effect on the strength of PF-->GC synapses. Unlike PCs, GCs were unable to regulate their PF synapses by releasing endocannabinoids. Moreover, PTP was reduced at the PF-->GC synapse compared to the PF-->PC synapse. Thus, the target-dependence of PF synapses arises from the differential expression of both retrograde signaling and PTP. 相似文献
6.
G. S. Boyan 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1999,184(3):279-294
Neuron 714 is morphologically one of the most prominent neurons in the central auditory pathway of the grasshopper with arborizations
extending from the abdominal neuromeres of the metathoracic ganglion to the brain. The aim of this study is to explore auditory
information flow involving neuron 714 at the level of the ventral nerve cord. Paired intracellular recordings were made from
neuron 714 in the mesothorax on the one hand, and from candidate presynaptic auditory neurons of the metathorax on the other.
Electrical stimulation of the tympanal nerves provides an estimate of the synaptic distance between these interneurons and
auditory afferents. Four, including neuron 714, are monosynaptically connected to afferents, the remainder disynaptically.
Current-injection and spike-triggered averaging reveal that of nine neurons examined, seven make either monosynaptic, disynaptic
or polysynaptic connections onto neuron 714. All connections are excitatory. Paired recordings show that response duration
and response amplitude in synaptically linked cells vary according to the frequency of the stimulus. Measurements of the latency
of the first excitatory post-synaptic potential evoked in neuron 714 by afferents and by metathoracic interneurons show how
the synaptic drive from these sources shapes the auditory response of neuron 714.
Accepted: 14 October 1998 相似文献
7.
8.
Barry A. Trimmer Janis C. Weeks 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1991,168(1):27-43
Summary In Manduca sexta larvae, sensory neurons innervating planta hairs on the tips of the prolegs make monosynaptic excitatory connections with motoneurons innervating proleg retractor muscles. Tactile stimulation of the hairs evokes reflex retraction of the proleg. In this study we examined activity-dependent changes in the amplitude of the excitatory postsynaptic potentials (EPSPs) evoked in a proleg motoneuron by stimulation of individual planta hair sensory neurons. Deflection of a planta hair caused a phasic-tonic response in the sensory neuron, with a mean peak instantaneous firing frequency of >300 Hz, and a tonic firing rate of 10–20 Hz. Direct electrical stimulation was used to activate individual sensory neurons to fire at a range of frequencies including those observed during natural stimulation of the hair. At relatively low firing rates (e.g., 1 Hz), EPSP amplitude was stable indefinitely. At higher instantaneous firing frequencies (>10 Hz), EPSPs were initially facilitated, but continuous stimulation led rapidly to synaptic depression. High-frequency activation of a sensory neuron could also produce post-tetanic potentiation, in which EPSP amplitude remained elevated for several min following a stimulus train. Facilitation, depression, and post-tetanic potentiation all appeared to be presynaptic phenomena. These activity-dependent changes in sensory transmission may contribute to the behavioral plasticity of the proleg withdrawal reflex observed in intact insects.Abbreviations
ACh
acetylcholine
-
AChE
acetylcholine esterase
-
CNS
central nervous system
-
EPSP
excitatory postsynaptic potential
-
I
h
injected hyperpolarizing current
-
LTP
long-term potentiation
-
PPR
principal planta retractor motoneuron
-
PTP
post-tetanic potentiation
-
R
in
input resistance
-
V
h
hyperpolarized potential
-
V
m
membrane potential
-
VN
ventral nerve
-
VNA
anterior branch of the ventral nerve
-
V
r
resting potential. 相似文献
9.
The modifiable reciprocal transcallosal monosynaptic excitatory connections were for the first time detected in vivo experiments in rat motor cortex using multiunit recording and crosscorrelation analysis, It was shown that high-frequency microstimulation (MCS) of a small group of cortical cells of one hemisphere produces long-term changes in the efficacy of transcallosal excitatory connections, and also ipsilateral connections in both hemispheres. The posttetanic changes appear as long-term potentiation (LTP) and long-term depression (LTD). The bursting neurons were found to have more favorable conditions for the induction of LTP of most converging inputs (in contrast to cells with other discharge patterns). Both LTP and LTD could be simultaneously induced in synapses formed by axon collaterals of a callosal cell on several neurons. LTP and LTD could be simultaneously obtained at diverse synapses of the same cell. The number of spontaneously active callosal neurons as well as the number and efficacy of transcallosal connections increased after the MCS, whereas the number and efficacy of ipsilateral connections decreased. Basing on these data we assume that the ipsilateral inhibition is more effective than the transcallosal inhibition. MCS results in the modification of the pattern of initially existing connections between numerous neurons of an ensemble including cells of both hemispheres. 相似文献
10.
Two second messenger pathways, one that uses the cAMP-dependent protein kinase A (PKA), the other that uses protein kinase C (PKC), have been found to contribute to the short-term presynaptic facilitation of the connections between the sensory neurons in Aplysia and their target cells, the interneurons and motor neurons of the gill-withdrawal reflex. To study their relative contributions as a function of the previous history of the neuron's activity, we have examined the effects of inhibiting PKA (using Rp-cAMPS) and PKC (using H7) on the short-term facilitation of spontaneous release as well as of the evoked release induced by serotonin at nondepressed, partially depressed, and highly depressed synapses. Our results suggest that whereas activation of PKA is sufficient to trigger the facilitation of nondepressed synapses, activation of both PKA and PKC is required to facilitate depressed synapses, with the contribution of PKC becoming progressively more important as synaptic transmission becomes more depressed. 相似文献
11.
12.
Layer 4 (L4) of primary visual cortex (V1) is the main recipient of thalamocortical fibers from the dorsal lateral geniculate nucleus (LGNd). Thus, it is considered the main entry point of visual information into the neocortex and the first anatomical opportunity for intracortical visual processing before information leaves L4 and reaches supra- and infragranular cortical layers. The strength of monosynaptic connections from individual L4 excitatory cells onto adjacent L4 cells (unitary connections) is highly malleable, demonstrating that the initial stage of intracortical synaptic transmission of thalamocortical information can be altered by previous activity. However, the inhibitory network within L4 of V1 may act as an internal gate for induction of excitatory synaptic plasticity, thus providing either high fidelity throughput to supragranular layers or transmittal of a modified signal subject to recent activity-dependent plasticity. To evaluate this possibility, we compared the induction of synaptic plasticity using classical extracellular stimulation protocols that recruit a combination of excitatory and inhibitory synapses with stimulation of a single excitatory neuron onto a L4 cell. In order to induce plasticity, we paired pre- and postsynaptic activity (with the onset of postsynaptic spiking leading the presynaptic activation by 10ms) using extracellular stimulation (ECS) in acute slices of primary visual cortex and comparing the outcomes with our previously published results in which an identical protocol was used to induce synaptic plasticity between individual pre- and postsynaptic L4 excitatory neurons. Our results indicate that pairing of ECS with spiking in a L4 neuron fails to induce plasticity in L4-L4 connections if synaptic inhibition is intact. However, application of a similar pairing protocol under GABAARs inhibition by bath application of 2μM bicuculline does induce robust synaptic plasticity, long term potentiation (LTP) or long term depression (LTD), similar to our results with pairing of pre- and postsynaptic activation between individual excitatory L4 neurons in which inhibitory connections are not activated. These results are consistent with the well-established observation that inhibition limits the capacity for induction of plasticity at excitatory synapses and that pre- and postsynaptic activation at a fixed time interval can result in a variable range of plasticity outcomes. However, in the current study by virtue of having two sets of experimental data, we have provided a new insight into these processes. By randomly mixing the assorting of individual L4 neurons according to the frequency distribution of the experimentally determined plasticity outcome distribution based on the calculated convergence of multiple individual L4 neurons onto a single postsynaptic L4 neuron, we were able to compare then actual ECS plasticity outcomes to those predicted by randomly mixing individual pairs of neurons. Interestingly, the observed plasticity profiles with ECS cannot account for the random assortment of plasticity behaviors of synaptic connections between individual cell pairs. These results suggest that connections impinging onto a single postsynaptic cell may be grouped according to plasticity states. 相似文献
13.
Using a realistic model of activity dependent dynamical synapse, which includes both depressing and facilitating mechanisms, we study the conditions in which a postsynaptic neuron efficiently detects temporal coincidences of spikes which arrive from N different presynaptic neurons at certain frequency f. A numerical and analytical treatment of that system shows that: (1) facilitation enhances the detection of correlated signals arriving from a subset of presynaptic excitatory neurons, and (2) the presence of facilitation yields to a better detection of firing rate changes in the presynaptic activity. We also observed that facilitation determines the existence of an optimal input frequency which allows the best performance for a wide (maximum) range of the neuron firing threshold. This optimal frequency can be controlled by means of facilitation parameters. Finally, we show that these results are robust even for very noisy signals and in the presence of synaptic fluctuations produced by the stochastic release of neurotransmitters. 相似文献
14.
Abramova MS Nistratova VL Moskvitin AA Pivovarov AS 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2005,55(3):385-392
Tetanic electric stimulation of Helix foot evokes sensitization of escape reaction. This behavioral sensitization and posttetanic potentiation (PTP) of acetylcholine-induced inward current (ACh-current) in command Helix neurons of escape behavior were similar. Antagonist of serotonin receptors methiothepin prevents the PTP of the ACh-current and behavioral sensitization. Serotonin disrupts the PTP of the ACh-current. It is suggested that the increase in cholinosensitivity of the command neurons with the involvement of methiothepin-sensitive serotonin receptors may be the cellular postsynaptic mechanism of behavioral sensitization of Helix escape reaction. 相似文献
15.
Maria Luisa Sotgiu Marco Brambilla Maurizio Valente Gabriele E.M. Biella 《Somatosensory & motor research》2013,30(3-4):211-215
Wide Dynamic Range (WDR) neurons in the spinal cord receive inputs from the contralateral side that, under normal conditions, are ineffective in generating an active response. These inputs are effective when the target WDRs change their excitability conditions. To further reveal the mechanisms supporting this effectiveness shift, we investigated the weight of the excitation of the contralateral neurons on the target WDR responses. In the circuit of presynaptic (sending) and postsynaptic (receiving) neurons in crossed spinal connections the fibres that form the presynaptic neurons impinge on postsynaptic neurons can be considered the final relay of this contralateral pathway. The enhancement of the presynaptic neuron excitability may thus modify the efficacy of the contralateral input. Pairs of neurons each on a side of the spinal cord, at the L5–L6 lumbar level were simultaneously recorded in intact, anaesthetized, paralysed rats. The excitatory aminoacid NMDA and strychnine, the antagonist of the inhibitory aminoacid glycine, were iontophoretically administrated to presynaptic neurons to increase their excitability. Before and during the drug administration, spontaneous and noxious-evoked activities of the neurons were analysed. During the iontophoresis of the two substances we found that noxious stimuli applied to the receptive field of presynaptic neurons activated up to 50% of the previously unresponsive postsynaptic neurons on the opposite side. Furthermore, the neurons on both sides of the spinal cord showed significantly increased spontaneous activity and amplified responses to ipsilateral noxious stimulation. These findings indicate that the contralateral input participates in the circuit dynamics of spinal nociceptive transmission, by modulating the excitability of the postsynaptic neurons. A possible functional role of such a nociceptive transmission circuit in neuronal sensitization following unilateral nerve injury is hypothesized. 相似文献
16.
Blanes cells mediate persistent feedforward inhibition onto granule cells in the olfactory bulb 总被引:4,自引:0,他引:4
Inhibitory local circuits in the olfactory bulb play a critical role in determining the firing patterns of output neurons. However, little is known about the circuitry in the major plexiform layers of the olfactory bulb that regulate this output. Here we report the first electrophysiological recordings from Blanes cells, large stellate-shaped interneurons located in the granule cell layer. We find that Blanes cells are GABAergic and generate large I(CAN)-mediated afterdepolarizations following bursts of action potentials. Using paired two-photon guided intracellular recordings, we show that Blanes cells have a presumptive axon and monosynaptically inhibit granule cells. Sensory axon stimulation evokes barrages of EPSPs in Blanes cells that trigger long epochs of persistent spiking; this firing mode was reset by hyperpolarizing membrane potential steps. Persistent firing in Blanes cells may represent a novel mechanism for encoding short-term olfactory information through modulation of tonic inhibitory synaptic input onto bulbar neurons. 相似文献
17.
Thomas Müller Stephanie Braud René Jüttner Birgit C Voigt Katharina Paulick Maria E Sheean Constantin Klisch Dilansu Gueneykaya Fritz G Rathjen Jörg RP Geiger James FA Poulet Carmen Birchmeier 《The EMBO journal》2018,37(17)
Hippocampal GABAergic interneurons are crucial for cortical network function and have been implicated in psychiatric disorders. We show here that Neuregulin 3 (Nrg3), a relatively little investigated low‐affinity ligand, is a functionally dominant interaction partner of ErbB4 in parvalbumin‐positive (PV) interneurons. Nrg3 and ErbB4 are located pre‐ and postsynaptically, respectively, in excitatory synapses on PV interneurons in vivo. Additionally, we show that ablation of Nrg3 results in a similar phenotype as the one described for ErbB4 ablation, including reduced excitatory synapse numbers on PV interneurons, altered short‐term plasticity, and disinhibition of the hippocampal network. In culture, presynaptic Nrg3 increases excitatory synapse numbers on ErbB4+ interneurons and affects short‐term plasticity. Nrg3 mutant neurons are poor donors of presynaptic terminals in the presence of competing neurons that produce recombinant Nrg3, and this bias requires postsynaptic ErbB4 but not ErbB4 kinase activity. Furthermore, when presented by non‐neuronal cells, Nrg3 induces postsynaptic membrane specialization. Our data indicate that Nrg3 provides adhesive cues that facilitate excitatory neurons to synapse onto ErbB4+ interneurons. 相似文献
18.
19.
Wide Dynamic Range (WDR) neurons in the spinal cord receive inputs from the contralateral side that, under normal conditions, are ineffective in generating an active response. These inputs are effective when the target WDRs change their excitability conditions. To further reveal the mechanisms supporting this effectiveness shift, we investigated the weight of the excitation of the contralateral neurons on the target WDR responses. In the circuit of presynaptic (sending) and postsynaptic (receiving) neurons in crossed spinal connections the fibres that form the presynaptic neurons impinge on postsynaptic neurons can be considered the final relay of this contralateral pathway. The enhancement of the presynaptic neuron excitability may thus modify the efficacy of the contralateral input. Pairs of neurons each on a side of the spinal cord, at the L5-L6 lumbar level were simultaneously recorded in intact, anaesthetized, paralysed rats. The excitatory aminoacid NMDA and strychnine, the antagonist of the inhibitory aminoacid glycine, were iontophoretically administrated to presynaptic neurons to increase their excitability. Before and during the drug administration, spontaneous and noxious-evoked activities of the neurons were analysed. During the iontophoresis of the two substances we found that noxious stimuli applied to the receptive field of presynaptic neurons activated up to 50% of the previously unresponsive postsynaptic neurons on the opposite side. Furthermore, the neurons on both sides of the spinal cord showed significantly increased spontaneous activity and amplified responses to ipsilateral noxious stimulation. These findings indicate that the contralateral input participates in the circuit dynamics of spinal nociceptive transmission, by modulating the excitability of the postsynaptic neurons. A possible functional role of such a nociceptive transmission circuit in neuronal sensitization following unilateral nerve injury is hypothesized. 相似文献
20.
Kenneth A. Jones Charles H. Page 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1986,158(2):273-280
The nature of the synaptic relationship between 7 identified postural interneurons and 5 pairs of superficial motoneurons was examined by obtaining dual intracellular recordings from interneuron-motoneuron pairs in the lobster 2nd abdominal ganglion. For six different interneuron-motoneuron pairs EPSPs recorded from motoneurons occurred with a short (1 to 3 ms) fixed latency following each presynaptic spike recorded from the interneuron. This suggests that there is a monosynaptic relationship between these interneurons and motoneurons. Monosynaptic pathways accounted for 27% of all excitatory connections. Preliminary evidence indicates that the monosynaptic potentials are mediated by an excitatory chemical synapse since: all IPSPs occurred with latencies greater than 5 ms, there was no evidence for electrical coupling, and one of the interneurons produced facilitating PSPs. A majority of all monosynaptic connections were made by two of the flexion producing interneurons (FPIs), 201 and 301. The synaptic outputs of these FPIs were similar in that both made monosynaptic connections with a different bilaterally homologous pair of motoneurons. Both also produced larger EPSPs and more vigorous spiking in contralateral members of the bilateral motoneuron pairs. A previous study demonstrated that interneurons 201 and 301 are the only postural interneurons yet identified that express motor programs indistinguishable from command neurons. Taken together, these results suggest that certain intersegmental interneurons share properties with command neurons and driver neurons, and that there may not be a sharp morphological or functional distinction between these two cell types. 相似文献