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宏基因组技术在开发未培养环境微生物基因资源中的应用 总被引:3,自引:0,他引:3
环境微生物宏基因组是一个巨大的基因资源库,但是仅有0.1%~1%的微生物在现有技术条件下是可培养的,因此致使未培养微生物基因资源的开发利用受到限制.宏基因组技术直接提取环境样品总DNA,避开了微生物分离培养的问题,极大扩展了微生物资源的利用空间,增加了获得新生物活性物质的机会.简要介绍了宏基因组的概念及宏基因组克隆技术的基本操作流程和技术要点,重点阐述了目的基因富集、核酸提取、载体和宿主系统选择、宏基因组文库筛选等"瓶颈"技术的研究进展.目的基因富集技术主要包括稳定同位素探针(SIP)、抑制消减杂交(SSH)和差异显示(DD)等.基因文库筛选分为序列依赖性筛选和非序列依赖性筛选,其中序列依赖性筛选包括特定基因PCR、反转录PCR (RT-PCR)、DNA微阵、亲和捕获等技术;非序列依赖性筛选主要指基于基因表达活性筛选和基因"陷阱"技术等.此外,介绍了一些近年来通过构建宏基因组文库筛选目的基因的应用实例. 相似文献
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采用传统分离培养筛选微生物新活性物质的方法受到很大制约,自然界99%以上的微生物不能培养,其资源开发受到很大限制。环境微生物宏基因组技术应用避开了微生物分离纯培养问题,极大拓展了微生物资源的利用空间,增加获得新活性物质的机会和途径。本文着重介绍宏基因组的概念、研究策略包括DNA提取、文库构建与筛选等及在微生物活性物质筛选中的应用,并对宏基因组研究中存在的问题进行探讨。 相似文献
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微生物蕴藏着大量具有工业应用潜力的生物催化剂。然而,传统培养方法只能从环境中获得不到1%的微生物。宏基因组学是通过提取某一特定环境中的所有微生物基因组DNA、构建基因组文库并对文库进行筛选,寻找和发现新的功能基因的一种方法。它绕过了微生物分离培养过程,成为研究环境样品中不可培养微生物的有力手段。因此,从宏基因组中挖掘新型生物催化剂一直倍受生物学家的关注。以下主要对宏基因组文库的样品来源、DNA提取方法、文库的构建和筛选策略的选择这4个方面的研究状况进行了综述,列举了近年来利用宏基因组技术所获得的新型生物催化剂,并对其今后的研究方向提出了展望。 相似文献
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宏基因组学诞生于上世纪90年代,是指不经过微生物培养阶段,采用直接提取环境中总DNA的方法,对微生物基因总和进行研究的一门新学科.宏基因组技术的出现,使得人们对占微生物总体99%以上不可培养微生物的研究成为现实,微生物基因的可探测空间显著增大.总的来说,目前宏基因组技术的应用主要分为两个方面:一方面是筛选功能基因,开发具有所需功能的蛋白;另一方面是通过对宏基因组文库进行分析,探讨在各种环境下微生物间相互作用和微生物与周围环境间相互影响的规律,以便我们能更加客观、全面地认识微生物世界.在宏基因组技术的应用范围被不断扩展的同时,围绕着宏基因组文库的构建和筛选、测序和分析等方面的研究已成为宏基因组学发展的主要推动力,宏基因组技术的进步将不断提升其应用价值. 相似文献
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宏基因组学( metagenome)是直接从土壤、海水、人及动物胃肠道、口腔、呼吸道、皮肤等环境中获取样品DNA,利用载体将其克隆到替代宿主细胞中构建宏基因文库,以高通量检测为主要技术来研究特定环境中全部微生物的基因组及筛选活性物质和基因的新兴学科。利用宏基因组学技术不仅能够有效地检测特定环境的微生物群落结构,扩展了微生物资源的利用空间,发展了新兴的高通量检测技术,丰富了生物信息学内容。基于宏基因组学研究方法在环境微生物研究中的优势,对近年来相关领域、方法及其在人及动物病原微生物研究中的应用进行综述,以期将此方法用于实验动物病原微生物的调查分析及动物疫情、生物安全的监测。 相似文献
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高通量筛选技术及其应用 总被引:7,自引:0,他引:7
主要介绍了高通量筛选技术(HTS,HighThroughputScreening)的原理,包括非细胞相筛选、细胞相筛选和生物表型筛选及其在生命科学和药学领域中的应用以及高通量筛选技术的发展趋势。 相似文献
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Metagenomes from uncultured microorganisms are rich resources for novel enzyme genes. The methods used to screen the metagenomic libraries fall into two categories, which are based on sequence or function of the enzymes. The sequence-based approaches rely on the known sequences of the target gene families. In contrast, the function-based approaches do not involve the incorporation of metagenomic sequencing data and, therefore, may lead to the discovery of novel gene sequences with desired functions. In this review, we discuss the function-based screening strategies that have been used in the identification of enzymes from metagenomes. Because of its simplicity, agar plate screening is most commonly used in the identification of novel enzymes with diverse functions. Other screening methods with higher sensitivity are also employed, such as microtiter plate screening. Furthermore, several ultra-high-throughput methods were developed to deal with large metagenomic libraries. Among these are the FACS-based screening, droplet-based screening, and the in vivo reporter-based screening methods. The application of these novel screening strategies has increased the chance for the discovery of novel enzyme genes. 相似文献
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人类基因组全序列的精细图已完成,当前生命科学面临的重要任务就是如何将基因组序列信息转化为基因的功能信息,了解生命活动的分子机理,改善人类健康,为生物技术发展提供动力 . 在一系列功能基因组研究新技术中,高通量 (high-throughput) 和高内涵 (high-content) 的细胞筛选技术平台已经显示出巨大潜力,发挥着越来越重要的作用 . 通过在体外培养的哺乳动物细胞中基因过表达或抑制基因表达,分析所产生信号传导通路和 / 或细胞表型改变,可以直接发现基因功能 . 近年来一些技术上的进展,使细胞筛选平台具有微量、自动、高效、高通量,以及可以系统研究的特点,已经成为功能基因组研究的核心方法之一 . 近 2~3 年来已经出现一批成功应用细胞筛选平台进行大规模功能基因组研究的报道 . 我国在这一领域的研究也开始起步,将对我国生物技术的源头创新研究产生深远的影响 . 相似文献
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ObjectiveIn France, the national breast cancer screening programme (NBCSP), targeting women aged 50–74 years was rolled out nationwide in 2004. It aims at reducing breast cancer mortality. In addition to the NBCSP, the use of opportunistic screening is permitted in France. The objective of this study is to estimate both opportunistic use and overall coverage rates of breast cancer screening, among women 40–84 years of age, in France.MethodsThe French medico-administrative health data system (SNDS) was used to identify women performing an opportunistic or organised mammography screening in France in 2016–2017.ResultsThe two-yearly opportunistic mammography screening is 18 % among women aged 40–84; it is 11 % among women aged 50–74, i.e., the target age range for organised screening, 36 % among women aged 40–49 and 13 % among women aged 75–84. The overall two-yearly screening coverage is 48 % for all women aged 40–84; it is 60 % among women aged 50–74, 36 % among women 40–49 and 16 % for those aged 75–84. Geographical variations in screening are lessened when the two screening strategies are considered, as they balance each other.ConclusionAlthough coverage in the NBCSP is around 50 % in France, more than one third of the women make use of opportunistic screening within and outside the target age range. Organized screening appears to improve equity of access to mammography screening service. The lack of data on opportunistic screening practices hinders the evaluation of French screening practices as a whole. 相似文献
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Costs and benefits of cervical screening. II. Is it worthwhile reducing the screening interval from 5 to 3 years? 总被引:1,自引:0,他引:1
A reduction in screening interval from 5 years to 3 years would greatly increase the cost of the programme, but would save few extra lives. The cost per life saved would be around £250 000 at 1995 prices, or around £8000 per life per year saved. There would in addition be human costs for the women screened. The opportunity cost of reducing the interval may be too great, since it is likely that the Health Service would achieve greater health benefits by investing the funds in other health care activities. 相似文献
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BackgroundIt is known that socioeconomic status (SES) influences the outcome of cancer treatment and this could partly be explained by decreased use of cancer screening services by people of lower SES. Many studies have indicated that low SES, including low educational attainment or unstable employment, was related to nonparticipation in cancer screening. However, studies investigating trends in SES inequalities within cancer screening participation are limited. Our objective was to examine trends in SES inequalities in cervical, breast, and colorectal cancer screening participation among women in Japan between 2010 and 2019.MethodsWe analyzed 189,442, 168,571, 163,341, and 150,828 women in 2010, 2013, 2016, and 2019 respectively, using nationally representative cross-sectional surveys. The main outcome variables are participation in each cancer screening. We used educational attainment and employment status as measures for SES. Multivariable logistic regression analysis, adjusted for age, marital status, educational attainment, and employment status was performed to evaluate the associations between SES and nonparticipation in each cancer screening.ResultsOverall participation rates in each cancer screening increased between 2010 and 2019. Low educational attainment and non-permanent employment status were related to nonparticipation in each cancer screening and inequality according to employment status increased within each screening participation during the study period. For example, dispatched workers were more likely to not participate in cervical cancer screening than permanent workers: in 2010, [aOR 1.11 95 %CI: 1.01 –1.21], and in 2019, [aOR 1.46 95 %CI: 1.34–1.60]. The inequality was greatest in colorectal cancer screening nonparticipation, followed by breast and cervical screening.ConclusionsAlthough the participation rates in each cancer screening have increased, inequality in participation in terms of employment status widened among women in Japan between 2010 and 2019. Reducing inequalities in cancer screening participation is essential for cancer screening intervention policies. 相似文献
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Rouslan V. Olkhov 《Analytical biochemistry》2010,396(1):30-2541
A biophotonic array based on gold nanoparticles functionalized with antigen proteins has been used to determine the concentrations of the respective antibodies in solution. Four proteins—fibrinogen, bovine serum albumin, transferrin, and C-reactive protein—were used to construct a test array with the assay repeated a number of times. The antibody-antigen association and dissociation rate constants were determined for the antibody assays from a series of calibration experiments. The label-free determination of the unknown antibody concentrations was performed using two related kinetic analyses. From these results, the current array assay sensitivity is 250 ng ml-1 with an accuracy of 15% using an 8-min kinetic measurement and a 16-spot averaged assay. 相似文献
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快速简便筛选cDNA文库的SSS法 总被引:5,自引:0,他引:5
建立了一种快速简便筛选cDNA文库的方法—SSS法(subsection screening)。该方法用cDNA噬菌体平板划块分组和根据目的基因设计的一对特异性引物,用PCR技术逐级筛选cDNA文库获得目的基因。与其它筛选cDNA文库的方法相比,该方法范围可控,目标明确,易于获得目的基因,而且快速、简捷、省时,一般可在一周内筛选出目的基因。本实验室利用该方法在半个月内筛选出了一个查耳酮异构酶(chalcone isomerase)(CHI)基因,一个黄酮类化合物3′-羟化酶(flavonoid 3′hydroxylase)(F3′H)基因,一个热激蛋白(heat shock protein)(HSP)基因和一个1 484 bp 热激蛋白基因片段。此方法用于同时筛选多个基因,可收到事半功倍的效果,对其它文库筛选也有借鉴意义。
Abstract:A quick and simple method subsection screening (SSS) method for screening cDNA library by PCR was established.With this method,cDNA phage plate was cut into several blocks and a couple of primers was designed according to target gene.And then the target genes were obtained by screening cDNA library.Comparing with other methods,this method has many advantages such as controlled range and clear target,and also quick and simple for obtaining the target genes.It is possible to get a target gene in one week in general by this method.Thus,the CHI gene,F3′H gene,HSP gene and one HSP partial fragment,were obtained respectively in half month in our lab.We can get twice the result with half the effort when we screen several genes in the same time.It is also suitable to screen other libraries. 相似文献
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Haselhorst T Münster-Kühnel AK Oschlies M Tiralongo J Gerardy-Schahn R von Itzstein M 《Biochemical and biophysical research communications》2007,359(4):866-870
We report an easy and direct application of 'Saturation Transfer Double Difference' (STDD) NMR spectroscopy to identify ligands that bind to a Sepharose-immobilised target protein. The model protein, cytidine 5'-monophosphate sialic acid (CMP-Sia) synthetase, was expressed as a Strep-Tag II fusion protein and immobilised on Strep-Tactin Sepharose. STD NMR experiments of the protein-enriched Sepharose matrix in the presence of a binding ligand (cytidine 5'-triphosphate, CTP) and a non-binding ligand (alpha/beta-glucose) clearly show that CTP binds to the immobilised enzyme, whereas glucose has no affinity. This approach has three major advantages: (a) only low quantities of protein are required, (b) no specialised NMR technology or the application of additional data analysis by non-routine methods is required, and (c) easy multiple use of the immobilised protein is available. 相似文献