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1.
Association mapping of yield and its components in rice cultivars 总被引:10,自引:0,他引:10
H. A. Agrama G. C. Eizenga W. Yan 《Molecular breeding : new strategies in plant improvement》2007,19(4):341-356
To make advances in rice breeding it is important to understand the relatedness and ancestry of introduced rice accessions,
and identify SSR markers associated with agronomically important phenotypic traits, for example yield. Ninety-two rice germplasm
accessions recently introduced from seven geographic regions of Africa, Asia, and Latin America, and eleven US cultivars,
included as checks, were evaluated for yield and kernel characteristics, and genotyped with 123 SSR markers. The SSR markers
were highly polymorphic across all accessions. Population structure analysis identified eight main clusters for the accessions
which corresponded to the major geographic regions, indicating agreement between genetic and predefined populations. Linkage
disequilibrium (LD) patterns and distributions are of fundamental importance for genome-wide mapping association. LD between
linked markers decreased with distance and with a substantial drop in LD decay values between 20 and 30 cM, suggesting it
should be possible to achieve resolution down to the 25 cM level. For the 103 cultivars, the complex traits yield, kernel
width, kernel length, kernel width/length ratio, and 1000-kernel weight, were estimated by analysis of variety trial data.
The mixed linear model method was used to disclose marker-trait associations. Many of the associated markers were located
in regions where QTL had previously been identified. In conclusion, association mapping in rice is a viable alternative to
QTL mapping based on crosses between different lines. 相似文献
2.
QTL mapping by association analysis has recently gained interest in plant breeding research as an alternative to QTL mapping in segregating populations from biparental crosses. In a first experiment on whole-genome association analysis in rapeseed, 684 mapped AFLP markers were tested for association with 14 traits in a set of 84 canola quality winter rapeseed cultivars. For association analysis a general linear model was used. By testing significance of marker-trait associations against a false discovery rate of 0.2, between 1 and 34 associated markers were found for 10 of the 14 traits. Taking into account linkage disequilibrium between the significant markers, these markers represent between 1 and 22 putative QTL for the respective traits. The minimum phenotypic variance explained by the QTL for the different traits ranged from 15% to 53%. A subset of 27 markers were significantly associated with two or more traits. These markers were predominantly shared between traits that were significantly correlated at the phenotypic level. The results show clearly that in rapeseed, QTL mapping by association analysis is a viable alternative to QTL mapping in segregating populations. 相似文献
3.
Comadran J Russell JR Booth A Pswarayi A Ceccarelli S Grando S Stanca AM Pecchioni N Akar T Al-Yassin A Benbelkacem A Ouabbou H Bort J van Eeuwijk FA Thomas WT Romagosa I 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(7):1363-1373
An association panel consisting of 185 accessions representative of the barley germplasm cultivated in the Mediterranean basin was used to localise quantitative trait loci (QTL) controlling grain yield and yield related traits. The germplasm set was genotyped with 1,536 SNP markers and tested for associations with phenotypic data gathered over 2?years for a total of 24?year?×?location combinations under a broad range of environmental conditions. Analysis of multi-environmental trial (MET) data by fitting a mixed model with kinship estimates detected from two to seven QTL for the major components of yield including 1000 kernel weight, grains per spike and spikes per m2, as well as heading date, harvest index and plant height. Several of the associations involved SNPs tightly linked to known major genes determining spike morphology in barley (vrs1 and int-c). Similarly, the largest QTL for heading date co-locates with SNPs linked with eam6, a major locus for heading date in barley for autumn sown conditions. Co-localization of several QTL related to yield components traits suggest that major developmental loci may be linked to most of the associations. This study highlights the potential of association genetics to identify genetic variants controlling complex traits. 相似文献
4.
de Oliveira Borba TC Brondani RP Breseghello F Coelho AS Mendonça JA Rangel PH Brondani C 《Genetics and molecular biology》2010,33(3):515-524
Association analysis was applied to a panel of accessions of Embrapa Rice Core Collection (ERiCC) with 86 SSR and field data from two experiments. A clear subdivision between lowland and upland accessions was apparent, thereby indicating the presence of population structure. Thirty-two accessions with admixed ancestry were identified through structure analysis, these being discarded from association analysis, thus leaving 210 accessions subdivided into two panels. The association of yield and grain-quality traits with SSR was undertaken with a mixed linear model, with markers and subpopulation as fixed factors, and kinship matrix as a random factor. Eight markers from the two appraised panels showed significant association with four different traits, although only one (RM190) maintained the marker-trait association across years and cultivation. The significant association detected between amylose content and RM190 was in agreement with previous QTL analyses in the literature. Herein, the feasibility of undertaking association analysis in conjunction with germplasm characterization was demonstrated, even when considering low marker density. The high linkage disequilibrium expected in rice lines and cultivars facilitates the detection of marker-trait associations for implementing marker assisted selection, and the mining of alleles related to important traits in germplasm. 相似文献
5.
Genome-wide association mapping of yield and yield components of spring wheat under contrasting moisture regimes 总被引:3,自引:0,他引:3
Erena A. Edae Patrick F. Byrne Scott D. Haley Marta S. Lopes Matthew P. Reynolds 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2014,127(4):791-807
Key message
A stable QTL that may be used in marker-assisted selection in wheat breeding programs was detected for yield, yield components and drought tolerance-related traits in spring wheat association mapping panel.Abstract
Genome-wide association mapping has become a widespread method of quantitative trait locus (QTL) identification for many crop plants including wheat (Triticum aestivum L.). Its benefit over traditional bi-parental mapping approaches depends on the extent of linkage disequilibrium in the mapping population. The objectives of this study were to determine linkage disequilibrium decay rate and population structure in a spring wheat association mapping panel (n = 285–294) and to identify markers associated with yield and yield components, morphological, phenological, and drought tolerance-related traits. The study was conducted under fully irrigated and rain-fed conditions at Greeley, CO, USA and Melkassa, Ethiopia in 2010 and 2011 (five total environments). Genotypic data were generated using diversity array technology markers. Linkage disequilibrium decay rate extended over a longer genetic distance for the D genome (6.8 cM) than for the A and B genomes (1.7 and 2.0 cM, respectively). Seven subpopulations were identified with population structure analysis. A stable QTL was detected for grain yield on chromosome 2DS both under irrigated and rain-fed conditions. A multi-trait region significant for yield and yield components was found on chromosome 5B. Grain yield QTL on chromosome 1BS co-localized with harvest index QTL. Vegetation indices shared QTL with harvest index on chromosome 1AL and 5A. After validation in relevant genetic backgrounds and environments, QTL detected in this study for yield, yield components and drought tolerance-related traits may be used in marker-assisted selection in wheat breeding programs. 相似文献6.
Improved Catharanthus roseus cultivars are required for high yields of vinblastine, vindoline and catharanthine and/or serpentine and ajmalicine, the pharmaceutical terpenoid indole alkaloids. An approach to derive them is to map QTL for terpenoid indole alkaloids yields, identify DNA markers tightly linked to the QTL and apply marker assisted selection. Towards the end, 197 recombinant inbred lines from a cross were grown over two seasons to characterize variability for seven biomass and 23 terpenoid indole alkaloids content-traits and yield-traits. The recombinant inbred lines were genotyped for 178 DNA markers which formed a framework genetic map of eight linkage groups (LG), spanning 1786.5 cM, with 10.0 cM average intermarker distance. Estimates of correlations between traits allowed selection of seven relatively more important traits for terpenoid indole alkaloids yields. QTL analysis was performed on them using single marker (regression) analysis, simple interval mapping and composite interval mapping procedures. A total of 20 QTL were detected on five of eight LG, 10 for five traits on LG1, five for four traits on LG2, three for one trait on LG3 and one each for different traits on LG three and four. QTL for the same or different traits were found clustered on three LG. Co-location of two QTL for biomass traits was in accord of correlation between them. The QTL were validated for use in marker assisted selection by the recombinant inbred line which transgressively expressed 16 traits contributory to the yield vinblastine, vindoline and catharanthine from leaves and roots that possessed favourable alleles of 13 relevant QTL. 相似文献
7.
Incorporating pleiotropic quantitative trait loci in dissection of complex traits: seed yield in rapeseed as an example 总被引:1,自引:0,他引:1
Ziliang Luo Meng Wang Yan Long Yongju Huang Lei Shi Chunyu Zhang Xiang Liu Bruce D. L. Fitt Jinxia Xiang Annaliese S. Mason Rod J. Snowdon Peifa Liu Jinling Meng Jun Zou 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2017,130(8):1569-1585
Key message
A comprehensive linkage atlas for seed yield in rapeseed.Abstract
Most agronomic traits of interest for crop improvement (including seed yield) are highly complex quantitative traits controlled by numerous genetic loci, which brings challenges for comprehensively capturing associated markers/genes. We propose that multiple trait interactions underlie complex traits such as seed yield, and that considering these component traits and their interactions can dissect individual quantitative trait loci (QTL) effects more effectively and improve yield predictions. Using a segregating rapeseed (Brassica napus) population, we analyzed a large set of trait data generated in 19 independent experiments to investigate correlations between seed yield and other complex traits, and further identified QTL in this population with a SNP-based genetic bin map. A total of 1904 consensus QTL accounting for 22 traits, including 80 QTL directly affecting seed yield, were anchored to the B. napus reference sequence. Through trait association analysis and QTL meta-analysis, we identified a total of 525 indivisible QTL that either directly or indirectly contributed to seed yield, of which 295 QTL were detected across multiple environments. A majority (81.5%) of the 525 QTL were pleiotropic. By considering associations between traits, we identified 25 yield-related QTL previously ignored due to contrasting genetic effects, as well as 31 QTL with minor complementary effects. Implementation of the 525 QTL in genomic prediction models improved seed yield prediction accuracy. Dissecting the genetic and phenotypic interrelationships underlying complex quantitative traits using this method will provide valuable insights for genomics-based crop improvement.8.
Quantitative trait locus-by-environment interaction for milk yield traits on Bos taurus autosome 6
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Lillehammer M Goddard ME Nilsen H Sehested E Olsen HG Lien S Meuwissen TH 《Genetics》2008,179(3):1539-1546
Genotype-by-environment interactions for production traits in dairy cattle have often been observed, while QTL analyses have focused on detecting genes with general effects on production traits. In this study, a QTL search for genes with environmental interaction for the traits milk yield, protein yield, and fat yield were performed on Bos taurus autosome 6 (BTA6), also including information about the previously investigated candidate genes ABCG2 and OPN. The animals in the study were Norwegian Red. Eighteen grandsires and 716 sires were genotyped for 362 markers on BTA6. Every marker bracket was regarded as a putative QTL position. The effects of the candidate genes and the putative QTL were modeled as a regression on an environmental parameter (herd year), which is based on the predicted herd-year effect for the trait. Two QTL were found to have environmentally dependent effects on milk yield. These QTL were located 3.6 cM upstream and 9.1 cM downstream from ABCG2. No environmentally dependent QTL was found to significantly affect protein or fat yield. 相似文献
9.
Validation of mega-environment universal and specific QTL associated with seed yield and agronomic traits in soybeans 总被引:1,自引:0,他引:1
Laura Palomeque Li-Jun Liu Wenbin Li Bradley R. Hedges Elroy R. Cober Mathew P. Smid Lewis Lukens Istvan Rajcan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2010,120(5):997-1003
The value of quantitative trait loci (QTL) is dependant on the strength of association with the traits of interest, allelic
diversity at the QTL and the effect of the genetic background on the expression of the QTL. A number of recent studies have
identified QTL associated with traits of interest that appear to be independent of the environment but dependant on the genetic
background in which they are found. Therefore, the objective of this study was to validate universal and/or mega-environment-specific
seed yield QTL that have been previously reported in an independent recombinant inbred line (RIL) population derived from
the cross between an elite Chinese and Canadian parent. The population was evaluated at two field environments in China and
in five environments in Canada in 2005 and 2006. Of the seven markers linked to seed yield QTL reported by our group in a
previous study, four were polymorphic between the two parents. No association between seed yield and QTL was observed. The
result could imply that seed yield QTL were either not stable in this particular genetic background or harboured different
alleles than the ones in the original mapping population. QTLU Satt162 was associated with several agronomic traits of which lodging was validated. Both the non-adapted and adapted parent
contributed favourable alleles to the progeny. Therefore, plant introductions have been validated as a source of favourable
alleles that could increase the genetic variability of the soybean germplasm pool and lead to further improvements in seed
yield and other agronomic traits. 相似文献
10.
Molecular-marker analysis of quantitative traits for growth and development in juvenile apple trees 总被引:1,自引:0,他引:1
P. J. Conner S. K. Brown N. F. Weeden 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(8):1027-1035
Random amplified polymorphic DNAs (RAPDs) were used in combination with a double pseudo-testcross mapping strategy to estimate
the position and effects of quantitative trait loci (QTLs) for traits influencing juvenile tree growth and development in
two apple cultivars. The mapping population consisted of 172 F1 trees from a cross between the columnar mutant ‘Wijcik McIntosh’ and a standard form disease-resistant selection NY 75441-58.
Significant associations were found between markers and height increment, internode number, internode length, base diameter
increment, base diameter after 9 years of growth, branch number, and leaf break. The number of genomic regions associated
with each trait varied from one to eight. The amount of variation explained by linear regression on individual marker loci
(R2) ranged from 3.9 to 24.3%, with an average of 7%. Multiple regression using markers for each putative QTL explained from
6.6 to 41.6% of the phenotypic variation, with an average value of 24.3%. A large number of traits had significant variation
associated with the map position of the dominant columnar gene, Co. QTL stability over years was estimated by comparing the locations of putative QTLs for traits measured in multiple years.
The majority of genomic regions were associated with a trait in only a single year, although regions associated with a trait
in more than 1 year were also detected. The limitations of dominant markers and an outbred mapping pedigree for QTL analysis
are discussed.
Received: 27 August 1997 / Accepted: 10 February 1998 相似文献
11.
Luis Inostroza Alejandro del Pozo Ivan Matus Dalma Castillo Patrick Hayes Stephen Machado Ann Corey 《Molecular breeding : new strategies in plant improvement》2009,23(3):365-376
Grain yield and plant height of 80 recombinant chromosome substitution lines (RCSLs) of barley were measured in six environments
with contrasting available moisture profiles. Two environments were in OR, USA (Moro and Pendleton) during one growing season
(2004), and four in Chile (Cauquenes and Santa Rosa) during two growing seasons (2004/2005 and 2007/2008). From the yield
data obtained in the different environments, yield adaptability (Finlay–Wilkinson slope) and stability (deviations from regression)
were calculated. Two commercial cultivars (Harrington and Baronesse) were used as checks in all environments. Marker-quantitative
trait associations were identified using 47 simple sequence repeats (SSRs) and the general linear model (GLM) implemented
in TASSEL. The mean plant height and grain yield of the 80 RCSLs differed greatly across environments, reflecting differences
in water availability. In all environments, there were significant differences (P < 0.05) in grain yield among RCSLs. There was also abundant variation in yield adaptability, indicating a differential response
of the RCSLs to environmental conditions across environments. Using principal component analysis, it was possible to identify
genotypes with better agronomic performance than the recurrent parent cv. Harrington. The association analysis revealed 21
chromosomal regions that were highly correlated with differences in grain yield, plant height and/or yield adaptability (Finlay–Wilkinson
slope). In approximately one-fourth of the cases, the H. spontaneum donor contributed favorable alleles. The associations were referenced to the quantitative trait loci (QTL) for the same traits
reported in the literature. 相似文献
12.
Pastina MM Malosetti M Gazaffi R Mollinari M Margarido GR Oliveira KM Pinto LR Souza AP van Eeuwijk FA Garcia AA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(5):835-849
Sugarcane-breeding programs take at least 12 years to develop new commercial cultivars. Molecular markers offer a possibility
to study the genetic architecture of quantitative traits in sugarcane, and they may be used in marker-assisted selection to
speed up artificial selection. Although the performance of sugarcane progenies in breeding programs are commonly evaluated
across a range of locations and harvest years, many of the QTL detection methods ignore two- and three-way interactions between
QTL, harvest, and location. In this work, a strategy for QTL detection in multi-harvest-location trial data, based on interval
mapping and mixed models, is proposed and applied to map QTL effects on a segregating progeny from a biparental cross of pre-commercial
Brazilian cultivars, evaluated at two locations and three consecutive harvest years for cane yield (tonnes per hectare), sugar
yield (tonnes per hectare), fiber percent, and sucrose content. In the mixed model, we have included appropriate (co)variance
structures for modeling heterogeneity and correlation of genetic effects and non-genetic residual effects. Forty-six QTLs
were found: 13 QTLs for cane yield, 14 for sugar yield, 11 for fiber percent, and 8 for sucrose content. In addition, QTL
by harvest, QTL by location, and QTL by harvest by location interaction effects were significant for all evaluated traits
(30 QTLs showed some interaction, and 16 none). Our results contribute to a better understanding of the genetic architecture
of complex traits related to biomass production and sucrose content in sugarcane. 相似文献
13.
《Small Ruminant Research》2009,82(2-3):146-151
Four genomic regions located on goat chromosomes 1, 2, 5 and 13 previously reported as significant DNA regions harboring quantitative trait loci (QTL) for growth and fleece traits in goat were considered as candidate genomic regions. The objective of conducting this study was to determine whether there is evidence for association of growth traits and cashmere yield in native Iranian Rayini goats with markers flanking these candidate regions. One hundred and twenty kids from 6 Rayini bucks (20 half-sib offspring per buck) were used in the present study. The six bucks and their offspring were genotyped for eight microsatellite markers flanking the candidate intervals. Body weights recorded on the kids were birth weight (BW0), weaning weight (BW3) and weight at 6 months of age (BW6). Average daily gains from birth to weaning and weaning to 6 months of age were computed based on the values obtained for BW0, BW3 and BW6. Cashmere yield was also recorded for the kids at the first, second and third year of age. QTL analysis in the candidate intervals was conducted using the least squared regression interval mapping approach. Linkage analysis indicated significant QTL for growth traits on goat chromosomes 1, 2 and 5 (chromosome-wide significance of P < 0.01). Three of the candidate regions located on chromosomes 2, 5 and 13 were linked to cashmere yield. QTL effect ranged from 0.8 to 4.3 in units of residual standard deviation for different traits and families. 相似文献
14.
Genetic control of yield related stalk traits in sugarcane 总被引:1,自引:0,他引:1
Aitken KS Hermann S Karno K Bonnett GD McIntyre LC Jackson PA 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2008,117(7):1191-1203
A major focus of sugarcane variety improvement programs is to increase sugar yield, which can be accomplished by either increasing
the sugar content of the cane or by increasing cane yield, as the correlation between these traits is low. We used a cross
between an Australian sugarcane variety Q165, and a Saccharum officinarum accession, IJ76-514, to dissect the inheritance of yield-related traits in the complex polyploid sugarcane. A population
of 227 individuals was grown in a replicated field trial and evaluated over 3 years for stalk weight, stalk diameter, stalk
number, stalk length and total biomass. Over 1,000 AFLP and SSR markers were scored across the population and used to identify
quantitative trait loci (QTL). In total, 27 regions were found that were significant at the 5% threshold using permutation
tests with at least one trait; individually, they explained from 4 to 10% of the phenotypic variation and up to 46% were consistent
across years. With the inclusion of digeneic interactions, from 28 to 60% of the variation was explained for these traits.
The 27 genomic regions were located on 22 linkage groups (LGs) in six of the eight homology groups (HGs) indicating that a
number of alleles or quantitative trait alleles (QTA) at each QTL contribute to the trait; from one to three alleles had an
effect on the traits for each QTL identified. Alleles of a candidate gene, TEOSINTE BRANCHED 1 (TB1), the major gene controlling branching in maize, were mapped in this population using either an SSR or SNP markers. Two alleles
showed some association with stalk number, but unlike maize, TB1 is not a major gene controlling branching in sugarcane but only has a minor and variable effect. 相似文献
15.
Mehrzad Eskandari Elroy R. Cober Istvan Rajcan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(6):1677-1687
Soybean [Glycine max (L.) Merrill] seed oil is the primary global source of edible oil and a major renewable and sustainable feedstock for biodiesel production. Therefore, increasing the relative oil concentration in soybean is desirable; however, that goal is complex due to the quantitative nature of the oil concentration trait and possible effects on major agronomic traits such as seed yield or protein concentration. The objectives of the present study were to study the relationship between seed oil concentration and important agronomic and seed quality traits, including seed yield, 100-seed weight, protein concentration, plant height, and days to maturity, and to identify oil quantitative trait loci (QTL) that are co-localized with the traits evaluated. A population of 203 F4:6 recombinant inbred lines, derived from a cross between moderately high oil soybean genotypes OAC Wallace and OAC Glencoe, was developed and grown across multiple environments in Ontario, Canada, in 2009 and 2010. Among the 11 QTL associated with seed oil concentration in the population, which were detected using either single-factor ANOVA or multiple QTL mapping methods, the number of QTL that were co-localized with other important traits QTL were six for protein concentration, four for seed yield, two for 100-seed weight, one for days to maturity, and one for plant height. The oil-beneficial allele of the QTL tagged by marker Sat_020 was positively associated with seed protein concentration. The oil favorable alleles of markers Satt001 and GmDGAT2B were positively correlated with seed yield. In addition, significant two-way epistatic interactions, where one of the interacting markers was solely associated with seed oil concentration, were identified for the selected traits in this study. The number of significant epistatic interactions was seven for yield, four for days to maturity, two for 100-seed weight, one for protein concentration, and one for plant height. The identified molecular markers associated with oil-related QTL in this study, which also have positive effects on other important traits such as seed yield and protein concentration, could be used in the soybean marker breeding programs aimed at developing either higher seed yield and oil concentration or higher seed protein and oil concentration per hectare. Alternatively, selecting complementary parents with greater breeding values due to positive epistatic interactions could lead to the development of higher oil soybean cultivars. 相似文献
16.
Li X Yan W Agrama H Jia L Shen X Jackson A Moldenhauer K Yeater K McClung A Wu D 《Planta》2011,234(2):347-361
Yield is the most important and complex trait for genetic improvement in crops, and marker-assisted selection enhances the
improvement efficiency. The USDA rice mini-core collection derived from over 18,000 accessions of global origins is an ideal
panel for association mapping. We phenotyped 203 O. sativa accessions for 14 agronomic traits and identified 5 that were highly and significantly correlated with grain yield per plant:
plant height, plant weight, tillers, panicle length, and kernels/branch. Genotyping with 155 genome-wide molecular markers
demonstrated 5 main cluster groups. Linkage disequilibrium (LD) decayed at least 20 cM and marker pairs with significant LD
ranged from 4.64 to 6.06% in four main groups. Model comparisons revealed that different dimensions of principal component
analysis affected yield and its correlated traits for mapping accuracy, and kinship did not improve the mapping in this collection.
Thirty marker–trait associations were highly significant, 4 for yield, 3 for plant height, 6 for plant weight, 9 for tillers,
5 for panicle length and 3 for kernels/branch. Twenty-one markers contributed to the 30 associations, because 8 markers were
co-associated with 2 or more traits. Allelic analysis of OSR13, RM471 and RM7003 for their co-associations with yield traits
demonstrated that allele 126 bp of RM471 and 108 bp of RM7003 should receive greater attention, because they had the greatest
positive effect on yield traits. Tagging the QTLs responsible for multiple yield traits may simultaneously help dissect the
complex yield traits and elevate the efficiency to improve grain yield using marker-assisted selection in rice. 相似文献
17.
R. Nagaraja Reddy R. Madhusudhana S. Murali Mohan D. V. N. Chakravarthi S. P. Mehtre N. Seetharama J. V. Patil 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(8):1921-1939
Sorghum, a cereal of economic importance ensures food and fodder security for millions of rural families in the semi-arid tropics. The objective of the present study was to identify and validate quantitative trait loci (QTL) for grain yield and other agronomic traits using replicated phenotypic data sets from three post-rainy dry sorghum crop seasons involving a mapping population with 245 F9 recombinant inbred lines derived from a cross of M35-1 × B35. A genetic linkage map was constructed with 237 markers consisting of 174 genomic, 60 genic and 3 morphological markers. The QTL analysis for 11 traits following composite interval mapping identified 91 QTL with 5–12 QTL for each trait. QTL detected in the population individually explained phenotypic variation between 2.5 and 30.3 % for a given trait and six major genomic regions with QTL effect on multiple traits were identified. Stable QTL across seasons were identified. Of the 60 genic markers mapped, 21 were found at QTL peak or tightly linked with QTL. A gene-based marker XnhsbSFCILP67 (Sb03g028240) on SBI-03, encoding indole-3-acetic acid-amido synthetase GH3.5, was found to be involved in QTL for seven traits. The QTL-linked markers identified for 11 agronomic traits may assist in fine mapping, map-based gene isolation and also for improving post-rainy sorghum through marker-assisted breeding. 相似文献
18.
A. Blanco G. Mangini A. Giancaspro S. Giove P. Colasuonno R. Simeone A. Signorile P. De Vita A. M. Mastrangelo L. Cattivelli A. Gadaleta 《Molecular breeding : new strategies in plant improvement》2012,30(1):79-92
Grain protein content (GPC) in durum wheat (Triticum turgidum var. durum) is negatively correlated with grain yield. To evaluate possible genetic interrelationships between GPC and grain yield per spike, thousand-kernel weight and kernel number per spike, quantitative trait loci (QTL) for GPC were mapped using GPC-adjusted data in a covariance analysis on yield components. Phenotypic data were evaluated in a segregating population of 120 recombinant inbred lines derived from crossing the elite cultivars Svevo and Ciccio. The material was tested at five environments in southern Italy. QTL were determined by composite interval mapping based on the Svevo?×?Ciccio linkage map described in Gadaleta et al. (2009) and integrated with DArT markers. The close relationship between GPC and yield components was reflected in the negative correlation between the traits and in the reduction of variance when GPC values were adjusted to yield components. Ten independent genomic regions involved in the expression of GPC were detected, six of which were associated with QTL for one or more grain yield components. QTL alleles with increased GPC effects were associated with QTL alleles with decreased effects on one or more yield component traits, or vice versa (i.e. the allelic effects were in opposite direction). Four QTL for GPC showed always significant effects, and these QTL should represent genes that influence GPC independently from variation in the yield components. Such genes are of special interest in wheat breeding since they would allow an increase in GPC without a concomitant decrease in grain yield. 相似文献
19.
S. Groh S. F. Kianian R. L. Phillips H. W. Rines D. D. Stuthman D. M. Wesenberg R. G. Fulcher 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(1):9-18
Milling yield, or the grain weight from which 100 kg of rolled groats is obtained upon milling, is an important quality characteristic
of cultivated oat (Avena sativa L.). Kernel morphology and the groat (caryopsis) percentage of the whole kernel including hull are factors that influence
milling yield. We mapped QTLs for kernel area, kernel length, kernel width, and groat percentage in two populations of 137
recombinant inbred lines by RFLP and AFLP analysis to evaluate the prospects of marker-assisted selection (MAS). Phenotypic
correlations between kernel morphology traits and groat percentage were not significant. For kernel morphology traits and
groat percentage, one to five QTLs were detected, explaining 7.0–60.7% of the total phenotypic variance depending on the trait.
One QTL for kernel length in each population and one QTL for kernel width in one population were found at the same location
as a QTL for groat percentage, indicating that a change in kernel size or shape could have an influence on groat percentage.
The positions and effects of QTLs for kernel morphology and groat percentage were compared to QTLs detected previously for
chemical grain composition (oil andβ-glucanconcentration) and agronomic traits to evaluate the selection response on these
traits through MAS. Several regions of the oat genome were identified that contained clusters of QTLs influencing two or more
traits. While the allele from one parent at a QTL could simultaneously improve two or more traits in one population, it could
have opposite effects on the same traits at another QTL or in the other population. Associations among traits were complex
and will require careful consideration when employing QTL-marker associations in MAS to avoid negative selection response.
Future research to discover candidate genes for those QTL clusters could provide information about trait associations and
help in designing selection programs.
Received: 17 February 2000 / Accepted: 27 October 2000 相似文献
20.
N. A. Alimi M. C. A. M. Bink J. A. Dieleman J. J. Magán A. M. Wubs A. Palloix F. A. van Eeuwijk 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(10):2597-2625