共查询到20条相似文献,搜索用时 15 毫秒
1.
K. C. Gupta Pradeep Kumar Dimpan Bhatia A. K. Sharma 《Nucleosides, nucleotides & nucleic acids》2013,32(3-5):829-832
Abstract A rapid method is described for the covalent anchoring of appropriately protected 2′-deoxyribonucleoside-3′-O-succinates to LCAA-CPG, widely used support for solid phase oligonucleotide synthesis. The method involves the reaction of nucleoside-3′-O-succinates with aminoalkyl functions of the support in the presence of improved and commercially available condensing reagent, TBTU or TPP-DTNP to generate fully functionalised polymer supports with excellent nucleoside loadings. 相似文献
2.
Gagan Dhawan Ramesh Chandra Kailash C. Gupta 《Nucleosides, nucleotides & nucleic acids》2015,34(3):149-162
In our previous report [Kumar, P.; Dhawan, G.; Chandra, R.; Gupta, K.C. Polyamine-assisted rapid and clean cleavage of oligonucleotides from cis-diol bearing universal support. Nucl. Acids Res. 2002, 30, e130 (1-8)], we demonstrated polyamine-mediated deprotection of oligonucleotides from cis-diol group bearing universal polymer support (I). However, vulnerability of the conventional dCbz to modifications under these conditions compelled us to employ dCac during synthesis of oligonucleotide using conventional synthons. Here, a new set of simple and rapid deprotection conditions has been developed for the complete cleavage of oligonucleotides from the 1,4-anhydroerythritol-based universal polymer support employing conventional dCbz synthon. Using manganese-imidazole complex in aqueous ammonium hydroxide (~30%), fully deprotected oligonucleotide sequences were obtained in 40 min, which were analyzed on reverse phase-HPLC and compared with the standard oligomers in terms of their retention time. Finally, their biological compatibility was established by analyzing PCR amplified products of npsA gene of N. meningitidis. 相似文献
3.
G. M. Bonora A. M. De Franco R. Rossin P. M. Veronese P. Ferrutic O. Plyasunova 《Nucleosides, nucleotides & nucleic acids》2013,32(8):1281-1288
Abstract A new amphiphilic, high-molecular weight poly (N-acryloylmorpholine) (PAcM) polymer has been used to be linked to oligonucleotide chains through a liquid-phase stepwise synthesis. This new conjugate has been investigated for its melting property, nuclease stability and capacity to elicit RNase H activity. Its antisense activity against an HIV-1 target has been also evaluated. 相似文献
4.
Abstract New variant of the manual method of oligonucleotide synthesis by “in situ” P(III) methodology will be presented. 相似文献
5.
Andres Jäschke Rolf Bald Eckhart Nordhoff Franz Hillenkamp Dieter Cech Volker A. Erdmann 《Nucleosides, nucleotides & nucleic acids》2013,32(9):1519-1529
Abstract Polyethylene glycols with degrees of polymerization from 5 to more than 100 were incorporated into synthetic oligoribonucleotides by automated solid phase synthesis at 3′-terminal, 5′-terminal and internal positions. The conjugates were characterized by chromatographic, electrophoretic and mass-spectrometric methods. The influence of coupling site, polymer size and number of coupled polymers per oligonucleotide on the molecular properties of the conjugates is investigated. 相似文献
6.
Kenneth D. James Guenter von Kiedrowski Andrew D. Ellington 《Nucleosides, nucleotides & nucleic acids》2013,32(10-11):1821-1836
Abstract An efficient method for synthesizing 5′-Boc-5′-amino-2′, 5′- dideoxynucleoside phosphoramidites and conditions for their incorporation in solid-phase oligonucleotide synthesis are presented. 相似文献
7.
Boleslaw Karwowski Piotr Guga Anna Kobylariska Wojciech J. Stec 《Nucleosides, nucleotides & nucleic acids》2013,32(9-11):1747-1759
Abstract Attempts at synthesis of “chimeric” oligonucleotide constructs (PO/PS-Oligos) possessing phosphate and P-stereodefined phosphorothioate internucleotide linkages via combined phosphoramidite/oxathiaphospholane methods were unsuccessful. Therefore, novel monomers for oxathiaphospholane method, namely 5′-O-DMT-deoxyribonucleoside 3′-O(2-oxo-.spiro-4.4-pentamethylene-1.3.2-oxathiaphospholane)s, were prepared and used together with their diastereomerically pure 2-thio analogues for the stereocontrolled synthesis of “chimeric” oligonucleotide constructs (PO/PS-Oligos). 相似文献
8.
J.-L. Barascut J. Cuartero J.-L. Imbach 《Nucleosides, nucleotides & nucleic acids》2013,32(3):193-202
Abstract A method of oligonucleotide synthesis was developped on a new type of support by the phosphotriester approach. Using this method the heptanucleotide dT(pT)6 was synthetized in 68 % yield. 相似文献
9.
Synthese d'Oligodesoxyribonucleotides sur Support de Gel de Silice par la Methode au Phosphotriester
Abstract A method of oligonucleotide synthesis was developped on a silica gel support by the phosphotriester approach. Using this method, the nonanucleoside octaphosphate dT(pT)8 was synthetized in 28% yield. 相似文献
10.
Abstract The synthesis of 3′-succinyl-CPG bound 3′,5′-di-2′-deoxythymidyl-(α-hydroxy-2-nitrobenzyl)-phosphonate diester 1 and the 3′-phosphoamidite derivative 2 is descibed. The hydroxyl-groups of the backbone modification were protected with trialkylsilyl groups: TES and TBS. Compounds 1, 2 are suitable blocks for oligonucleotide synthesis. 相似文献
11.
Vasulinga T. Ravikumar Tadeusz K. Wyrzykiewicz Douglas L. Cole 《Nucleosides, nucleotides & nucleic acids》2013,32(8):1695-1706
Abstract An indirect method for investigating evidence of the formation of full length deadenylated phosphorothioate oligonucleotide (ISIS 2105) as a degradation product is reported through synthesis of its deadenylated analog. Alternatively, use of Leumann's method for the synthesis of authentic deadenylated ISIS 2105 using the tert-butyldimethylsilyl protecting group at the anomeric hydroxyl function of the furanose ring failed in our hands. 相似文献
12.
Novel Base-Labile Protecting Groups for 5′-Hydroxy Function in Solid-Phase Oligonucleotide Synthesis
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1011-1015
Abstract The 6-(levulinyloxymethyl)-3-methoxy-2-nitrobenzoyl (LMMoNBz) and 2-(levulinyloxymethyl)-5-methoxy-4-nitrobenzoyl (LMMpNBz) groups were developed as novel base-labile protection for the 5′-hydroxy function in solid-phase oligonucleotide synthesis. A comparative study of the LMMoNBz, LMMpNBz and 2-(levulinyloxymethyl)-5-nitrobenzoyl (LMNBz) protecting groups for oligonucleotide synthesis proved strong feasibility for the LMMoNBz group. 相似文献
13.
《Nucleosides, nucleotides & nucleic acids》2013,32(5-8):1403-1406
Abstract A novel photocleavable universal support for the automated solid phase synthesis of oligonucleotides is described. The linker between the growing oligonucleotide chain and CPG support contains a nucleophilic amine protected with a photocleavable group. On exposure to UV light, this group is detached and the free amine affords cleavage of the oligonucleotide from the support. The use of long wavelength UV light avoids damage to the DNA. 相似文献
14.
Zhaoda Zhang Alexandra Nichols Jimmy X. Tang Mazen Alsbeti Jin Yan Tang 《Nucleosides, nucleotides & nucleic acids》2013,32(7-9):1585-1588
Abstract Several sulfur-transfer reagents have been evaluated for large scale synthesis of oligonucleotide phosphorothioate analogues, in which 3-ethoxy-1, 2-dithiazoline-5-one (EDITH, 5) shows potential as an alternative to Beaucage reagent. 相似文献
15.
P. Kumar N. N. Ghosh K. L. Sadana B. S. Garg K. C. Gupta 《Nucleosides, nucleotides & nucleic acids》2013,32(6):565-584
Abstract A more convenient and efficient method is described for the preparation of 3′-O-succinates of 2′-deoxyribo- and ribonucleosides, in a variety of solvents. Also, a new one-pot procedure has been developed for anchoring these succinates to polymer supports, suitable for solid phase synthesis of oligonucleotides. 相似文献
16.
Ajay Kumar 《Nucleosides, nucleotides & nucleic acids》2013,32(5):441-447
Abstract A novel method for the synthesis of oligonucleotides with terminal-3′-phosphate using universal CPG polymer support is described. 相似文献
17.
Synthesis and Properties of O-β-D-Ribofuranosyl-(1″-2′)-Adenosine-5″-O-Phosphate and Its Derivatives
Andrei A. Rodionov Ekaterina V. Efimtseva Sergey N. Mikhailov Jef Rozenski Ingrid Luyten Piet Herdewijn 《Nucleosides, nucleotides & nucleic acids》2013,32(10-12):1847-1859
Abstract The synthesis of O-β-D-ribofuranosyl-(1″-2′)-adenosine-5″-O-phosphate and its suitably protected derivative for oligonucleotide synthesis have been developed. 相似文献
18.
Abstract A novel phosphoramidite building block derived from N-chloroacetyl-6-aminohexanol is attached at the 5′-terminus on the last step of oligonucleotide synthesis. Postsynthetic treatment of support-bound modified oligonucleotides with a variety of amines and mercaptanes affords oligonucleotide conjugates in a high yield. 相似文献
19.
Ajay Kumar Hema Dawar Sudhakar Mathur 《Nucleosides, nucleotides & nucleic acids》2013,32(9):1575-1578
Abstract A simple, rapid and novel method for purification of the oligonucleotide using silica gel matrix is described. 相似文献
20.
Abstract The establishment of a new synthesis procedure for the preparation of oligonucleotide arrays is described. A modified phosphoramidite chemistry allowed the in situ synthesis of oligomer arrays on specially derivatized polypropylene membranes which can be used both for hybridisation experiments and for the isolation of the individual oligonucleotides. 相似文献